Overexpression of fj^Scer\UAS.cZa under the control of Scer\GAL4^salm-459.2 does not affect wing hair polarity.

Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in clones in the dorsal adult abdomen results in the reversal of hairs in front of the clone.

Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb-Gal4 results in weak polarity reversals in the eye disc.

Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 results in loss of the anterior crossvein and subtle change sin wing hair polarity (wing hairs point towards the region overexpressing fj^Scer\UAS.cZa mainly in the distal part of the wing).

The wings of ft^Scer\UAS.cMa; Scer\GAL4^en-e16E flies have planar polarity defects in much of the posterior compartment and a a posterior crossvein which has moved into distally, into line with the anterior crossvein. ft^Scer\UAS.cMa; Scer\GAL4^salm-459.2 have a similarly misplace posterior cross-vein, with severe planar cell polarity defects clustered around it. ft^Scer\UAS.cMa; Scer\GAL4^ap-md544 lack a posterior crossvein, have an incomplete anterior crossvein and scattered strong planar polarity defects. ft^Scer\UAS.cMa; Scer\GAL4^ptc-559.1 flies also have a distally displaced posterior crossvein with planar polarity defects immediately proximal and distal to it.

Flies expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a fj^d1/fj^N7 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 92.9% of ommatidia are correctly constructed and rotated in these animals. Expression of fj^Scer\UAS.cZa under the control of either Scer\GAL4^αTub84B.PL or Scer\GAL4^fj-VG1 has little effect on ommatidial polarity, with only occasional mis-specified ommatidia being seen. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb3-GAL4 has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb3-GAL4 in a fj^d1/fj^N7 background has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen.

fj^Scer\UAS.cZa; Scer\GAL4^bi-omb-Gal4 flies have shorter wings with anterior and posterior crossveins closer together than wild-type. These flies have eyes with several rows of inverted ommatidia at their dorsal and ventral edges. fj^Scer\UAS.cZa; Scer\GAL4^Act5C.PI somatic clones in the eye cause non-autonomous inversions of one or two rows of ommatidia on the equatorial side of the clone.

Within the anterior compartments of the adult abdominal cuticle, somatic clones in which fj^Scer\UAS.cZa is expressed under the control of the Scer\GAL4^Ubx.PdC only exhibit planar polarity defects when they are located in the posterior of the compartment. Within the clone there are whorls, and these can extend outside the clone in the anterior, but not the posterior direction. Clones within the posterior compartment appear normal. Polarity defects result when fj^Scer\UAS.cZa is expressed under the control of the Scer\GAL4^αTub84B.PL in somatic clones in the adult abdominal cuticle (generated by loss of Scer\GAL80^αTub84B.PL through mitotic recombination): In the anterior compartment clones rarely have whorls, and the hairs are reversed within the anterior part of the clone. This reversal extends anterior to the clone itself by as much as 6 or 7 cell diameters. However, some clones within the extreme anterior portion of the hair producing territory of the anterior compartment cause little or no changes in polarity anterior to the clone. Clones in the posterior compartment are also associated with polarity reversals: hairs within the posterior half of the clone as well as hairs behind are reversed and now point anteriorly. When a clone in the anterior compartment abuts the posterior compartment boundary then non-autonomous reversal of hair polarity is seen both anterior and posterior to the clone.

Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^69B results in ectopic joint-like structures in the tarsal segments, loss of proximal-distal growth within the tarsal segments and loss of the joint between tarsal segments 2 and 3. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 results in significant truncation and fusion of tarsal segments. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^dpp.blk1 results in leg truncations and fusion of tarsal segments, as well as cuticular abnormalities and occasional outgrowths from the leg. Some of these outgrowths appear to be segmented and most have at least some bristles. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^en-e16E results in leg truncations and outgrowths. Clones in the leg expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^Act5C.PI that span a segment border result in the fusion of most segments. In the tarsus, fusion of the tarsal segments is accompanied by reduced growth. Loss of joint structures is seen both within and adjacent to the clone. Occasional outgrowths from the leg are seen in legs containing larger clones. These outgrowths are composed entirely of wild-type tissue, with clone tissue being close to the outgrowth.

When fj^Scer\UAS.cZa is driven by Scer\GAL4^{Scer\FRT.Act5C} occasional swirls in the wing hair pattern are seen in the proximal regions in the wing. The overall wing length is shortened, specifically the distal wing is shortened but the proximal wing size remains unaffected. These flies also have defects in the leg - including proximal-distal shortening and tarsal fusion. Scer\GAL4^{Scer\FRT.Act5C} driven expression also produces wing vein phenotypes, including loss of the posterior crossvein and displacement of vein 4 at the junction with the wing margin as well as the deletion of the anterior cross vein in the region. When Scer\GAL4^ptc-559.1 drives expression of fj^Scer\UAS.cZa in a stripe along the anterior margin of the anterior/posterior compartment boundary of the developing wing, nearby wing hairs rotate towards the highest levels of fj expression close to the compartment boundary. When Scer\GAL4^ptc-559.1 drives expression of fj^Scer\UAS.cZa in the leg, extreme proximal-distal axis shortening and loss of distal structures, while the coxa, a proximal structure, appears largely unaffected.

When fj^Scer\UAS.cZa is driven at the poles of the eye by Scer\GAL4^bi-omb-Gal4, dorsoventral inversions of ommatidial polarity are seen at poles of the eye, often with 3 or more rows inverted. When driven by Scer\GAL4^{Scer\FRT.Act5C} ommatidial inversions are seen on the equatorial boundary of expression. When driven by Scer\GAL4^ey.PH no phenotype is seen. When driven by Scer\GAL4^hs.2sev or Scer\GAL4^ey.PH occasional ommatidia are seen with inverted polarity relative to their neighbours. Tese phenotypes are similar but stronger than the homozygous mutant fj phenotypes.

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has visible phenotype

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has abnormal planar polarity phenotype

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has visible phenotype

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has abnormal planar polarity phenotype

Scer\GAL4^dpp.blk1, Wnt4^UAS.cGa, fj^UAS.cZa has abnormal planar polarity phenotype

Scer\GAL4^en-e16E, fj^UAS.cZa has wing hair | posterior phenotype, suppressible | partially by ds⁰⁵¹⁴²/ds⁰⁵¹⁴²

Scer\GAL4^en-e16E, fj^UAS.cZa has wing blade posterior compartment phenotype, suppressible | partially by ds⁰⁵¹⁴²/ds⁰⁵¹⁴²

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has wing hair phenotype

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has wing hair phenotype

Scer\GAL4^dpp.blk1, Wnt4^UAS.cGa, fj^UAS.cZa has wing hair phenotype