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Allele Dmel\btd^Scer\UAS.cSa

General Information
Symbol	Dmel\btdScer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Schock	FlyBase ID	FBal0103887
Feature type	allele	Associated gene	Dmel\btd
Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Schock et al., 1999)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference
Carried in construct	P{UAS-btd.S} (Estella et al., 2003, Schock et al., 1999, Franch-Marro et al., 2006, Estella and Mann, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	homeotic | somatic clone, with Scer\GAL4tub.PU (Estella and Mann, 2010) visible, with Scer\GAL4sca-537.4 (Schock et al., 1999)
Phenotype Manifest In
	antenna, with Scer\GAL4ey.PH (Estella et al., 2003) antenna | ectopic, with Scer\GAL4ey.PH (Estella et al., 2003) eye, with Scer\GAL4ey.PH (Estella et al., 2003) haltere, with Scer\GAL4bi-omb-Gal4 (Estella et al., 2003) haltere, with Scer\GAL4Dll-MD743 (Estella et al., 2003) haltere, with Scer\GAL4nub-AC-62 (Estella et al., 2003) haltere | somatic clone, with Scer\GAL4Act5C.PP (Estella et al., 2003) Keilin's organ, with Scer\GAL4btd-MD808 (Franch-Marro et al., 2006) leg | distal | ectopic | somatic clone, with Scer\GAL4tub.PU (Estella and Mann, 2010) leg | ectopic, with Scer\GAL4bi-omb-Gal4 (Estella et al., 2003) leg | ectopic, with Scer\GAL4Dll-MD743 (Estella et al., 2003) leg | ectopic, with Scer\GAL4nub-AC-62 (Estella et al., 2003) leg | ectopic | proximal | somatic clone, with Scer\GAL4tub.PU (Estella and Mann, 2010) leg | ectopic | somatic clone, with Scer\GAL4Act5C.PP (Estella et al., 2003) wing, with Scer\GAL4bi-omb-Gal4 (Estella et al., 2003) wing, with Scer\GAL4Dll-MD743 (Estella et al., 2003) wing, with Scer\GAL4nub-AC-62 (Estella et al., 2003) wing, with Scer\GAL4sca-537.4 (Schock et al., 1999) wing | somatic clone, with Scer\GAL4Act5C.PP (Estella et al., 2003) wing | somatic clone, with Scer\GAL4tub.PU (Estella and Mann, 2010) wing margin bristle, with Scer\GAL4sca-537.4 (Schock et al., 1999) wing margin bristle | ectopic, with Scer\GAL4sca-537.4 (Schock et al., 1999)
Detailed Description
	Statement Reference Clones expressing btd[Scer\UAS.cSa], generated under the control of Scer\GAL4[tub.PU] using the MARCM system, show ectopic leg tissues in the wing. Both proximal and distal leg structures can be seen. (Estella and Mann, 2010) Expression of btdScer\UAS.cSa under the control of Scer\GAL4btd-MD808 causes the Keilin's organs to develop more than the the wild-type number of three sensory hairs. (Franch-Marro et al., 2006) When btdScer\UAS.cSa is driven by Scer\GAL4ey.PH, the original eye disappears and is replaced by an ectopic antenna, which often fuses with the normal one. When btdScer\UAS.cSa is driven by Scer\GAL4nub-AC-62, wings and halteres differentiate leg tissue. The leg tissue in these flies is not arranged in segments but it is possible to recognise pattern elements distal markers such as claws are found as well as tarsal and tibia-like bristles. The amount of leg tissue produced in each of these appendages is greater than the corresponding individual legs. When btdScer\UAS.cSa is driven by Scer\GAL4MD743 or Scer\GAL4bi-omb-Gal4 sets of leg structures are seen in the wing disc derivatives. btdScer\UAS.cSa clones (driven by Scer\GAL4Act5C.PP) in the haltere and wing differentiate leg tissue. These clones normally appear as either vesicles of tissue segregated from the wing and the haltere, or as protruding tissue. They tend to form leg patterns resembling normal ones, which include the formation of various leg segments. Moreover, these clones develop nearly complete leg patterns independently of the wing region where they localise. (Estella et al., 2003) Ectopic expression of btdScer\UAS.cSa driven by Scer\GAL4sca-537.4 causes development of mechanosensory stout bristles in ectopic places in the wing. (Schock et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4tub.PU, btdScer\UAS.cSa has homeotic | somatic clone phenotype, suppressible | partially by Dll17 (Estella and Mann, 2010)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4tub.PU, btdScer\UAS.cSa has leg | distal | ectopic | somatic clone phenotype, suppressible by Dll17 (Estella and Mann, 2010) Scer\GAL4tub.PU, btdScer\UAS.cSa has wing | somatic clone phenotype, suppressible | partially by Dll17 (Estella and Mann, 2010)
NOT suppressed by
Statement Reference Scer\GAL4tub.PU, btdScer\UAS.cSa has leg | proximal | ectopic | somatic clone phenotype, non-suppressible by Dll17 (Estella and Mann, 2010)
Other
Statement Reference DllScer\UAS.cGa, Scer\GAL4btd-MD808, btdScer\UAS.cSa has Keilin's organ | ectopic phenotype (Franch-Marro et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference A Dll[17] mutant background partially suppresses the ectopic leg tissue phenotype seen when btd[Scer\UAS.cSa] clones are generated under the control of Scer\GAL4[tub.PU] using the MARCM system. These clones cannot generate distal leg fates, but are able to produce what appears to be proximal leg tissue. (Estella and Mann, 2010) Coexpression of btdScer\UAS.cSa and DllScer\UAS.cGa, under the control of Scer\GAL4btd-MD808, cause ectopic formation of Keilin's organs in otherwise normal abdominal segments. These ectopic organs have more than the wild-type number of three sensory hairs. (Franch-Marro et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	Scer\GAL4Dll-em212/btdScer\UAS.cSa fails to rescue Df(1)btd-Sp1 (Estella and Mann, 2010) Scer\GAL4tub.PU/btdScer\UAS.cSa fails to rescue Df(1)btd-Sp1 (Estella and Mann, 2010)
Comments	Expression of btd[Scer\UAS.cSa] (using the MARCM system under the control of Scer\GAL4[tub.PU]) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae. Expression of btd[Scer\UAS.cSa] fails to rescue the growth defects seen in Df(1)btd-Sp1 mutant leg clones generated in a minute background during embryogenesis using Scer\FLP1[Scer\UAS.cUa] under the control of Scer\GAL4[Dll-em212]. (Estella and Mann, 2010)
Stocks ( 1 )
Bloomington	26881 w*; wgSp-1/CyO; P{UAS-btd.S}M1
Notes on Origin
Discoverer
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	btdScer\UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Schock (Schock et al., 1999)
Secondary FlyBase IDs
References ( 4 )
Research paper	Estella and Mann, 2010, PLoS Genet. 6(6): e1001001 Non-Redundant Selector and Growth-Promoting Functions of Two Sister Genes, buttonhead and Sp1, in Drosophila Leg Development. [FBrf0211142] Franch-Marro et al., 2006, Development 133(5): 785--790 Association of tracheal placodes with leg primordia in Drosophila and implications for the origin of insect tracheal systems. [FBrf0190289] Estella et al., 2003, Development 130(24): 5929--5941 The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila. [FBrf0167505] Schock et al., 1999, Mech. Dev. 89(1-2): 125--132 Common and diverged functions of the Drosophila gene pair D-Sp1 and buttonhead. [FBrf0112032]