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General Information
Symbol
Dmel\Su(H)del47
Species
D. melanogaster
Name
FlyBase ID
FBal0103950
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Su(H)Δ47, Su(H)047, Su(H)D47, Su(H)Δ47
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:
Imprecise excision of P{}Su(H)12, resulting in the substitution of a 1881bp fragment (from -1145 to +737bp, relative to the Su(H) transcription start site) with 9 unrelated nucleotides. This deletion removes both Su(H) and CIAPIN1 transcribed sequences. Boundaries approximate; not clear what transcription start was used.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of the P-element, resulting in the substitution of a 1881bp fragment (from -1145 to +737bp, relative to the Su(H) transcription start site) with 9 unrelated nucleotides. This deletion removes both Su(H) and l(2)35Bg transcribed sequences.
Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
dorsal mesothoracic disc & neuron
neuron & eye | somatic clone
neuron & eye | supernumerary | somatic clone
photoreceptor cell R8 & eye disc | ectopic | somatic clone
photoreceptor cell R8 & eye disc | somatic clone
Detailed Description
Statement
Reference
The induction of Su(H)del47 somatic clones leads to a severe increase in the adult posterior midgut mitotic index in females only, as compared to controls.
Su(H)del47 homozygous lateral antennal lobe neuroblast clones, induced at the newly-hatched larval stage, have fewer antennal mechanosensory and motor center neurite projection tracts and 3 times the number of later-born uniglomerular lateral antennal lobe projection neurons in the adult; clones have similar total numbers of cells to controls by adulthood and clone size and cell division (PH3 staining) are unaffected at 30 and 70 hours after larval hatching.
Multilayering of intestinal stem cells and enteroendocrine cells is seen in homozygous clones in the adult intestine.
Su(H)del47 mutant clones generated in type II neuroblasts are smaller than control clones. In contrast, clones that originate in type I clones are similar in size to controls.
Homozygous clones in the outer proliferation center neuroepithelium (induced at late-first or early-second instar and analysed at late-third instar) adopt an irregular cell morphology rather than the normal columnar epithelial cell morphology in 78.6% of cases.
Homozygous intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells.
Su(H)del47 clones in the wing disc are relatively small and scattered over the disc, with very rugged edges. They are associated with a large number of dead cells in the basal side.
Follicle stem cell clones homozygous for Su(H)del47 persist in the ovariole approximately as well as control clones over a 14 day period.
Homozygous intestinal stem cell (ISC) clones in the adult midgut form tumours.
The dorsal/ventral boundary is established correctly in Su(H)del47 mutant wing imaginal discs.
Su(H)del47 mutant germline stem cells are maintained in the niche similarly to wild-type control clones. Su(H)del47 clones of cap cells and escort stem cells result in a reduction of region 1 of the germaria.
Su(H)del47 mutant cells do not enter the second mitotic wave.
Mitotic clones of cells carrying Su(H)del47 rarely contribute to the presumptive anterior dorsal-ventral region of the eye disc.
R8 photoreceptor differentiation is normal or early in Su(H)del47 single mutant clones. In addition, many ectopic R8 photoreceptors differentiate in these clones.
Mutant wing discs exhibit a lack of neural differentiation. The proneural clusters appear to arrest their development during an early phase of sensory organ precursor development. When mutant clones are made in the wing disc, a delay in the development of the pDC sensory organ precursors is seen.
Homozyogous somatic clones in the adult eye are neurogenic. Mutant cells also differentiate prematurely.
Large homozygous mutant clones that presumably include the polar cell precursors lead to fusions between adjacent egg chambers. Mutant epithelial follicle cells continue to divide after stage 6. When mutant clones include the follicle cells at the posterior of the egg chamber, the germinal vesicle often fails to migrate and remains at the posterior pole.
Su(H)12/Su(H)del47 animals have a rough eye phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Su(H)del47 has proneural cluster phenotype, non-enhanceable by PsnC1
Suppressed by
Statement
Reference
Su(H)del47 has photoreceptor cell R8 & eye disc | ectopic | somatic clone phenotype, suppressible | partially | somatic clone by ci94
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Su(H)del47 is an enhancer of ommatidium phenotype of aopA141
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Su(H)del47/+;CycGHR7/CycGHR7 flies display no phenotypic defects in adult wings.
The suppression of the WGMR.PG eye phenotype seen in eyes mosaic for Pka-C1B3 (generated using the ey-FLP/FRT system) is partially reverted if these clones are also mutant for Su(H)del47.
Su(H)del47 suppresses the presence of ectopic NB-like cells (Dpn and Mira positive) phenotype seen when Nint.G.Scer\UAS clones are induced in third instar larval type II NBs under the control of Scer\GAL4αTub84B.PL.
HE31 Su(H)del47 double mutant intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells, as occurs in Su(H)del47 single mutant clones.
Expression of armS10.Scer\UAS.T:Hsap\MYC via Scer\GAL4αTub84B.PL in Su(H)del47 clones (using the MARCM technique) increases the size and reduces the number of apoptotic cells compared to Su(H)del47 clones. armS10.Scer\UAS.T:Hsap\MYC expression also makes the clones more rounded in appearance but cells do not lose their polarity.
Su(H)del47/+ suppresses the wing vein thickening seen in dx152 hemizygotes.
In contrast to PsnC1 single mutants, the dorsal/ventral boundary forms correctly in Su(H)del47; PsnC1 double mutants. apUGO35/aprK568; HE31 and apUGO35/aprK568; Su(H)del47/Su(H)8 wing discs fail to form a dorsal/ventral compartment boundary. Unlike expression in clones with a wild-type background, expression of dxScer\UAS.cMa (driven by Scer\GAL4αTub84B.PP) in Su(H)del47 mutant clones does not result in increased cell proliferation.
Eye disc cells simultaneously mutant for Mad12, ci94 and Su(H)del47 continue proliferating instead of arresting in G1 ahead of the morphogenetic furrow. The same phenotype is seen in cells mutant for Mad12 and ci94 - the presence of Su(H)del47 has no autonomous effect on G1 arrest. Eye disc cells simultaneously mutant for Mad1-2, ci94 and Su(H)del47 arrest in G1 after a severe delay but they never enter the second mitotic wave. Eye disc cells simultaneously mutant for Su(H)del47 and Mad1-2, or Su(H)del47 and ci94, do not enter the second mitotic wave. No second mitotic wave is observed in Su(H)del47, Sosx122 clones.
R8 photoreceptor differentiation is delayed in ci94; Su(H)del47 double mutant clones, and fails in ci94; Mad1-2; Su(H)del47 triple mutant clones. This is despite normal R8 differentiation in any of the single mutants (although it is sometimes early in Su(H)del47 single mutant clones), and in Mad1-2; Su(H)del47 double mutants. Ectopic R8 photoreceptor differentiation in ci94; Su(H)del47 somatic clones in eye discs, is much reduced compared to that seen in Su(H)del47 single mutant clones. This is not the case with Mad1-2; Su(H)del47 double mutant clones.
Xenogenetic Interactions
Statement
Reference
The wing vein L5 thickening characteristic for adult females expressing Hsap\ATXN1LScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hh.PU can be suppressed by combination with Su(H)del47/+, while the loss of posterior crossvein is only partially restored.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (6)
Notes on Origin
Discoverer
Selected as: a strong dominant suppressor of the H haplo-insufficient bristle phenotype.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
References (45)