Allele Dmel\Sec61β¹⁴⁴⁴⁸

General Information
Symbol	Dmel\Sec61β¹⁴⁴⁴⁸	Species	D. melanogaster
Name		FlyBase ID	FBal0103965
Feature type	allele	Created / Updated	2006-05-15/2006-05-15
Associated gene	Dmel\Sec61β

Allele class
Mutagen	P-element activity
Nature of the Allele
Allele class
Mutagen	P-element activity (Valcarcel et al., 1999)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacW} insertion 29bp downstream of the putative transcription start site. (Valcarcel et al., 1999)
Assay mode
Caused by insertion	P{lacW}Sec61β¹⁴⁴⁴⁸ (Valcarcel et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| germ-line clone \| non-rescuable maternal effect \| recessive (Valcarcel et al., 1999)
Phenotype Manifest In
	embryonic/first instar larval cuticle (Valcarcel et al., 1999) denticle belt (Valcarcel et al., 1999) epicuticle & embryo (Valcarcel et al., 1999) endocuticle & embryo (Valcarcel et al., 1999) embryonic/larval trachea (Valcarcel et al., 1999) dorsal appendage \| germ-line clone (Valcarcel et al., 1999) eye \| somatic clone (Valcarcel et al., 1999) ommatidium \| somatic clone (Valcarcel et al., 1999) rhabdomere \| somatic clone (Valcarcel et al., 1999) tarsal segment \| somatic clone (Valcarcel et al., 1999) leg \| somatic clone (Valcarcel et al., 1999)
Detailed Description
	Statement Reference Homozygotes die at the end of embryonic development (stage 17) with a very thin cuticle in which the denticle belts are hardly visible. After removal of the vitelline membrane, the embryos can hardly move and die after a few minutes. There are no obvious patterning defects in the cuticle. The protein epicuticle layer is absent in mutant embryos and the thickness of the endocuticle is reduced. Microvilli can be seen at the surface of the epidermis in the mutant embryos. The distribution of the trachea is abnormal. Eggs derived from homozygous female germ-line clones have egg shells that show defects in dorsal-ventral patterning; the dorsal appendages are either separated by a smaller distance than normal, partially fused or completely fused. The distribution of egg shell phenotypes is similar in if the females are mated to either wild-type males or males carrying Sec61β¹⁴⁴⁴⁸. The embryos derived from homozygous female germ-line clones do not hatch, and die at a variety of developmental stages, with only a few reaching stages 16 or 17 at 25^oC. The stage of lethality varies with temperature; at 18^oC, 34.4% reach stages 16 and 17. The embryos have a very thin cuticle. The phenotype cannot be paternally rescued. Approximately 5% of ommatidia either lack photoreceptors or have morphological defects in homozygous clones in the eye. The number of rhabdomeres seen is often lower in apical sections than in more basal sections, suggesting defects in differentiation that cause the rhabdomeres not to form over the entire apical-basal extent of the retina. Photoreceptors are only rarely completely missing. Homozygous clones in the wing do not show any visible defects. Homozygous clones in the leg are associated with a severe reduction in size of the tarsal segments. This reduction in size often occurs distal to the clone. (Valcarcel et al., 1999)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Sec61β⁰⁷²¹⁴ (Valcarcel et al., 1999) Sec61β^k07836 (Valcarcel et al., 1999)
Rescued by	Sec61β¹⁴⁴⁴⁸ is rescued by Sec61β^Tub.PV (Valcarcel et al., 1999)
Comments	Lethality is rescued by Sec61β^Tub.PV. (Valcarcel et al., 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	The lethality and cuticle phenotype of Sec61β¹⁴⁴⁴⁸ is revertible by P-element excision. (Valcarcel et al., 1999)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DSec61β^P1 (Valcarcel et al., 1999) Sec61β¹⁴⁴⁴⁸
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Valcarcel et al., 1999, J. Cell Sci. 112(23): 4389--4396 Sec61(), a subunit of the protein translocation channel, is required during Drosophila development. [FBrf0112063]

Allele Dmel\Sec61β14448

Allele Dmel\Sec61β¹⁴⁴⁴⁸