FlyBase Allele Report: Scer\GAL4[s.gcm]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^s.gcm

General Information

Symbol

Scer\GAL4^s.gcm

Species

S. cerevisiae

Name

FlyBase ID

FBal0103970

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

sgcmGAL4, gcm-gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Booth et al., 2000)

Progenitor genotype

Carried in construct

Cytology

Description

11 repeats of the consensus gcm protein binding sequence are fused upstream of an Hsp70 promoter which drives GAL4 expression.

(Booth et al., 2000)

Allele components

Component

Use(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

glial cell | variable

(Hidalgo and Booth, 2000)

(Hidalgo et al., 2001)

neuroblast MP2 | variable

(Booth et al., 2000)

embryonic/larval plasmatocyte | variable

(Hidalgo and Booth, 2000)

glioblast | variable

(Hidalgo and Booth, 2000)

embryonic stage 12 -- 17

cell body glial cell | lateral | variable

(Booth et al., 2000)

glioblast | lateral | variable

(Booth et al., 2000)

glial cell | lateral | variable

(Booth et al., 2000)

CNS surface glial cell | lateral | variable

(Booth et al., 2000)

embryonic stage 13

embryonic/larval plasmatocyte | variable

(Booth et al., 2000)

embryonic stage 14 -- 16

glial cell | variable

(Kinrade et al., 2001)

embryonic stage 16

neuron | variable

(Booth et al., 2000)

embryonic stage 16 -- 17

neuron | variable

(Hidalgo and Booth, 2000)

Additional Information

Statement

Reference

Embryonic expression is observed in a mosaic fashion in glia.

(Kinrade et al., 2001)

Expression is mosaic, affecting only a few glioblasts and glial cells per embryo.

(Hidalgo et al., 2001)

Expression is mosaic, seen in only a few surface and cortex glioblasts per embryo, and is prior to axon extension. Sporadic expression is also observed in neurons at embryonic stage 16 and in some, but not all, macrophages.

(Booth et al., 2000)

Expression is mosaic, seen in only a few surface and cortex glioblasts per embryo, and is prior to axon extension. Sporadic expression is also observed in neurons, most often MP2s, and in some, but not all, macrophages.

(Booth et al., 2000)

Expression is observed in the glioblast progenitor of the longitudinal glia, and its progeny, and in other glial classes in a mosaic fashion. Sporadic expression is observed in some macrophages and in neurons after embryonic stage 16.

(Hidalgo and Booth, 2000)

Marker for

CNS surface glial cell

(Booth et al., 2000)

cell body glial cell

(Booth et al., 2000)

(Kinrade et al., 2001)

(Hidalgo et al., 2001, Booth et al., 2000, Hidalgo and Booth, 2000)

Reflects expression of

(Hidalgo et al., 2001, Kinrade et al., 2001, Booth et al., 2000, Hidalgo and Booth, 2000)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Rcom\RT^A.UAS

(Booth et al., 2000)

abnormal neuroanatomy, with zfh1^UAS.cLa

(Layden et al., 2006)

Phenotype Manifest In

dMP2 neuron, with Rcom\RT^A.UAS

(Hidalgo and Booth, 2000)

embryonic/larval hemocyte, with Pvr^DN.UASp

(Olofsson and Page, 2005)

embryonic glioblast, with Rcom\RT^A.UAS

(Hidalgo and Booth, 2000)

embryonic longitudinal glial cell, with Rcom\RT^A.UAS

(Booth et al., 2000, Hidalgo and Booth, 2000)

fascicle, with Rcom\RT^A.UAS

(Booth et al., 2000)

fascicle | embryonic stage, with Rcom\RT^A.UAS

(Hidalgo and Booth, 2000)

fascicle | embryonic stage, with Rcom\RT^A.UAS, Scer\GAL4^15J2

(Hidalgo and Booth, 2000)

larval DA1 motor neuron, with Rcom\RT^A.UAS

(Booth et al., 2000, Hidalgo and Booth, 2000)

larval glial cell, with Rcom\RT^A.UAS

(Booth et al., 2000)

larval longitudinal connective, with Rcom\RT^A.UAS

(Booth et al., 2000, Hidalgo and Booth, 2000)

larval longitudinal connective, with Rcom\RT^A.UAS, Scer\GAL4^15J2

(Hidalgo and Booth, 2000)

larval longitudinal connective, with robo1³, robo1^UAS.cKa

(Kinrade et al., 2001)

larval MP1 neuron, with Rcom\RT^A.UAS

(Hidalgo and Booth, 2000)

larval neuron, with Rcom\RT^A.UAS

(Booth et al., 2000)

larval RP2 motor neuron | ectopic, with Rcom\RT^A.UAS

(Booth et al., 2000)

larval ventral nerve cord | embryonic stage, with Pvr^DN.UASp

(Olofsson and Page, 2005)

motor nerve & axon, with zfh1^UAS.cLa

(Layden et al., 2006)

pCC neuron, with Rcom\RT^A.UAS

(Booth et al., 2000)

pCC neuron, with Rcom\RT^A.UAS, Scer\GAL4^15J2

(Hidalgo and Booth, 2000)

vMP2 neuron, with Rcom\RT^A.UAS

(Hidalgo and Booth, 2000)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rcom\RT^A.UAS, Scer\GAL4^s.gcm has abnormal cell death phenotype, suppressible by BacA\p35^elav.PB

(Booth et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rcom\RT^A.UAS, Scer\GAL4^s.gcm has larval neuron phenotype, suppressible by BacA\p35^elav.PB

(Booth et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^s.gcm/robo1^UAS.cKa partially rescues robo1³

(Kinrade et al., 2001)

Fails to rescue

Scer\GAL4^s.gcm/zfh1^UAS.cLa fails to rescue zfh1⁵

(Layden et al., 2006)

Scer\GAL4^s.gcm/zfh1^UAS.cLa fails to rescue zfh1⁵/zfh1^75.26

(Layden et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^s.gcm

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections