FlyBase Allele Report: Dmel\HDAC1[303]

General Information

Symbol

Dmel\HDAC1³⁰³

Species

D. melanogaster

Name

FlyBase ID

FBal0104003

Feature type

allele

Associated gene

Dmel\HDAC1

Associated Insertion(s)

Carried in Construct

Also Known As

rpd3³⁰³

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Mottus et al., 2000)

Mutagen

Progenitor genotype

Cytology

Description

The mutated residue C98 is perfectly conserved in homologs from S. cerevisiae to H. sapiens, in a region of the protein that is almost perfectly conserved, and that is thought to be required for deacetylase activity. Lesions in HDAC1³⁰³ and HDAC1³²⁸, which were independently isolated, are identical.

(Mottus et al., 2000)

Amino acid replacement: C98Y.

(Mottus et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,627,747..4,627,747 [+]

Nucleotide change:

G4627747A

Amino acid change:

C98Y | HDAC1-PA

Reported amino acid change:

C98Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Mottus et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Tea et al., 2010)

alar lobe

(Mottus et al., 2000)

macrochaeta

(Mottus et al., 2000)

sex comb

(Mottus et al., 2000)

wing

(Mottus et al., 2000)

Detailed Description

Statement

Reference

Homozygous single-cell DL1 projection neuron clones mistarget their dendrites towards dorsomedial (82% of clones) or ventromedial regions (14% of clones) of the antennal lobe (normally the dendrites of this projection neuron project to the dorsolateral glomerulus DL1).

(Tea et al., 2010)

Rpd3³⁰³/+ flies have the normal number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

One copy of Rpd3³⁰³ strongly suppresses position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of Rpd3³⁰³ weakly suppresses the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

Strong suppressor of PEV in In(1)w^m4. Raises the pigment levels of In(1)w^m4 eyes from 5-15% of wild type levels to 60-90% of wild type levels. When raised under carefully maintained culture conditions transheterozygotes of the genotype HDAC1⁰⁴⁵⁵⁶/HDAC1³⁰³ adult males eclose at 40% the expected rate and females at 30% the expected rate. Adults are short lived and females produce few eggs, apparently unfertilised. Phenotypes include strong suppression of In(1)w^m4 variegation, severely notched wings, small, malformed, sometimes curved or duplicated bristles, allulae that are larger than normal, and a reduction in the number of sex combs. Recessive lethality acts equally in the third larval instar or during pupation. Shows a dominant semi-lethal effect on males: viability 71% of expected.

(Mottus et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

HDAC1³⁰³/HDAC1[+] is an enhancer of abnormal circadian rhythm | adult stage | dominant phenotype of CoRest^MI08173

(Lv et al., 2024)

Suppressor of

Statement

Reference

HDAC1³⁰³ is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Other

Statement

Reference

Df(3R)sbd45/+, HDAC1³⁰³ has visible | dominant phenotype

(Canudas et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

HDAC1³⁰³/Rpd3[+] is an enhancer of dorsocentral bristle | increased number phenotype of pnr^D1

(Biryukova and Heitzler, 2008)

HDAC1³⁰³ is an enhancer of sex comb phenotype of Pc⁴

(Chang et al., 2001)

Suppressor of

Statement

Reference

HDAC1³⁰³ is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Other

Statement

Reference

Df(3R)sbd45/+, HDAC1³⁰³ has adult abdominal segment 6 phenotype

(Canudas et al., 2005)

Df(3R)sbd45/+, HDAC1³⁰³ has abdominal sternite bristle | ectopic phenotype

(Canudas et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The disorganised eye phenotype caused by expression of sens^Scer\UAS.cNa under the control of Scer\GAL4^lz-gal4 is suppressed by Rpd3³⁰³.

(Anderson et al., 2011)

Flies heterozygous for Rpd3³⁰³ enhance the phenotype of pnr^D1/+, resulting in extra sensory organs.

(Biryukova and Heitzler, 2008)

96% of Rpd3³⁰³/Df(3R)sbd45 transheterozygous males show transformation of A6 to A5, as judged by the presence of at least one bristle on the sixth sternite.

(Canudas et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

HDAC1^15-1

(Mottus et al., 2000)

Fails to complement

HDAC1³¹³

(Mottus et al., 2000)

HDAC1³²⁸

(Mottus et al., 2000)

HDAC1⁰⁴⁵⁵⁶

(Mottus et al., 2000)

HDAC1³²⁶

(Mottus et al., 2000)

Rescued by

HDAC1³⁰³ is rescued by HDAC1^UAS.Tag:V5/Scer\GAL4^GH146

(Tea et al., 2010)

Comments

Expression of Rpd3^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GH146 in single-cell DL1 projection neuron clones which are mutant for Rpd3³⁰³ significantly rescues the dendrite mistargeting defects seen in these clones.

(Tea et al., 2010)

Transheterozygotes of Rpd3^15-1 with Rpd3³⁰³, Rpd3³¹³, Rpd3³²⁶ or Rpd3³²⁸ are viable and fertile, and show weak to moderate suppression of In(1)w^m4 PEV.

(Mottus et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32240

w^*; HDAC1³⁰³ P{FRT(w^hs)}2A P{neoFRT}82B/TM6B, Tb¹

26791

HDAC1³⁰³/T(2;3)CyO-TM3, P{GAL4-Hsp70.PB}TR1, P{UAS-GFP.Y}TR1: P{GAL4-Hsp70.PB}TR2, P{UAS-GFP.Y}TR2, y⁺ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

HDAC1³⁰³

(Lv et al., 2024, Biryukova and Heitzler, 2008, Mottus et al., 2000)

HDAC³⁰³

(Canudas et al., 2005)

Hdac1³⁰³

(Doheny et al., 2008, Chang et al., 2001)

Rpd3³⁰³

(Muraoka et al., 2021, Morimoto et al., 2016, Anderson et al., 2011, Tea et al., 2010, Chen et al., 2009)

rpd3³⁰³

(Lewis et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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