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General Information
Symbol
Dmel\PtenUAS.cGa
Species
D. melanogaster
Name
FlyBase ID
FBal0104062
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dPTEN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of Pten isoform 3.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The dorsal hole is elongated compared to wild type during dorsal closure in embryos expressing PtenScer\UAS.cGa under the control of Scer\GAL4αTub84B.PL. Cells expressing PtenScer\UAS.cGa under the control of Scer\GAL4en.PU show a significant decrease in zippering speed during dorsal closure compared to neighbouring control cells. This results in the mutant cells often zippering later than more central wild-type cells, leaving transient gaps in the zippering seam. The number of filopodia at the leading edge is significantly reduced in the mutant cells.
Expression of PtenScer\UAS.cGa driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.
Expression of ThorT37A.T46A.Scer\UAS under the control of Scer\GAL4ci.PU results in a reduction in total wing area compared to control flies. A reduction in area of both the wing compartment expressing the ThorT37A.T46A.Scer\UAS transgene and the neighbouring, non-expressing wing compartment is seen. Expression of PtenScer\UAS.cGa under the control of Scer\GAL4salm.PU results in wings with smaller wings than normal, without a large impact of the overall size of the animal (adult weight and pupal length are unchanged compared to controls). A clear increase in the number of TUNEL-positive cells is seen in the territory of PtenScer\UAS.cGa expression in wing discs expressing PtenScer\UAS.cGa under the control of Scer\GAL4en-e16E, as well as in adjacent cells that are not expressing PtenScer\UAS.cGa.
Hemocytes in PtenScer\UAS.cGa; Scer\GAL4srp.Hemo late embryos do not exhibit abnormal aggregation.
When expression is driven by Scer\GAL4dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size. When expression is driven by Scer\GAL4en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.
When expression is driven by Scer\GAL4dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size. When expression is driven by Scer\GAL4en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of Rac1Scer\UAS.cLa suppresses the decrease in zippering speed during dorsal closure seen in cells expressing PtenScer\UAS.cGa under the control of Scer\GAL4en.PU. The reduction in number of filopodia at the leading edge is also suppressed.
Co-expression of PtenScer\UAS.cGa and Dcp-1Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) under the control of Scer\GAL4GMR.PF is lethal.
Co-expression of Sesnd04539 with PtenScer\UAS.cGa driven by Scer\GAL4ap-md544 enhances the wing-bending phenotype of PtenScer\UAS.cGa.
Flies expressing both Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX and PtenScer\UAS.cGa driven by Scer\GAL4en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing PtenScer\UAS.cGa driven by Scer\GAL4en-e16E alone.
Flies expressing both Pi3K92EScer\UAS.T:Hsap\MYC and PtenScer\UAS.cGa driven by Scer\GAL4en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing PtenScer\UAS.cGa driven by Scer\GAL4en-e16E alone.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PtenScer\UAS.cGa
PtenUAS.cGa
Name Synonyms
Secondary FlyBase IDs
    References (8)