The dorsal hole is elongated compared to wild type during dorsal closure in embryos expressing
PtenScer\UAS.cGa under the control of
Scer\GAL4αTub84B.PL.
Cells expressing
PtenScer\UAS.cGa under the control of
Scer\GAL4en.PU show a significant decrease in zippering speed during dorsal closure compared to neighbouring control cells. This results in the mutant cells often zippering later than more central wild-type cells, leaving transient gaps in the zippering seam. The number of filopodia at the leading edge is significantly reduced in the mutant cells.
Expression of
ThorT37A.T46A.Scer\UAS under the control of
Scer\GAL4ci.PU results in a reduction in total wing area compared to control flies. A reduction in area of both the wing compartment expressing the
ThorT37A.T46A.Scer\UAS transgene and the neighbouring, non-expressing wing compartment is seen.
Expression of
PtenScer\UAS.cGa under the control of
Scer\GAL4salm.PU results in wings with smaller wings than normal, without a large impact of the overall size of the animal (adult weight and pupal length are unchanged compared to controls).
A clear increase in the number of TUNEL-positive cells is seen in the territory of
PtenScer\UAS.cGa expression in wing discs expressing
PtenScer\UAS.cGa under the control of
Scer\GAL4en-e16E, as well as in adjacent cells that are not expressing
PtenScer\UAS.cGa.
When expression is driven by
Scer\GAL4dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size. When expression is driven by
Scer\GAL4en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.
When expression is driven by
Scer\GAL4dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size.
When expression is driven by
Scer\GAL4en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.
