Allele Dmel\Pten³

General Information
Symbol	Dmel\Pten³	Species	D. melanogaster
Name		FlyBase ID	FBal0104063
Feature type	allele	Associated gene	Dmel\Pten

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Goberdhan et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia \| somatic clone (Goberdhan et al., 1999, Hietakangas and Cohen, 2007) lethal \| late third instar larval stage (with Df(2L)flp170B) (Vereshchagina et al., 2008) lethal \| recessive (Goberdhan et al., 1999)
Phenotype Manifest In
	crossvein \| somatic clone (Goberdhan et al., 1999) eye \| somatic clone (Goberdhan et al., 1999, Hietakangas and Cohen, 2007) interommatidial bristle \| somatic clone (Goberdhan et al., 1999) ommatidium \| somatic clone (Goberdhan et al., 1999) photoreceptor cell \| cell autonomous \| somatic clone (Goberdhan et al., 1999) rhabdomere \| cell autonomous \| somatic clone (Goberdhan et al., 1999) wing & cell \| somatic clone (Goberdhan et al., 1999) wing \| somatic clone (Goberdhan et al., 1999) wing hair \| ectopic \| somatic clone (Goberdhan et al., 1999)
Detailed Description
	Statement Reference Homozygous clones in the eye disc result in strong overgrowth of the eye. (Hietakangas and Cohen, 2007) Hemizygotes die as late embryos or early first instar larvae. Homozygous mutant clones in the eye bulge out from the eye surface. Ommatidia sometimes fuse interommatidial bristles are abnormal, particularly (but not exclusively) in the dorsal half of the eye. Mutant clones typically contain at least twice as many ommatidia as their wild type twin spots. The largest clones can form a hyperplastic tumor-like overgrowth, though this phenotype is less severe than for tumor suppressor mutations. Within each ommatidium cell type specification seems normal. Cell bodies of mutant photoreceptors are enlarged compared to wild type neighbors, and the rhabdomeres are misshapen. The cell size and rhabdomeric phenotypes are cell autonomous. Homozygous mutant clones in the wing reveal an increase in cell size. Wing hairs are occasionally duplicated and crossvein formation is abnormal. Occasional extra wing vein material develops. Longitudinal veins are rarely affected. (Goberdhan et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Df(2L)flp170B/Pten³ has lethal \| late third instar larval stage phenotype, suppressible \| partially by Akt1[+]/Akt1¹ (Vereshchagina et al., 2008) Df(2L)flp170B/Pten³ has lethal \| late third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^arm.PS/gig^Scer\UAS.cGa/Tsc1^Scer\UAS.cGa (Vereshchagina et al., 2008) Df(2L)flp170B/Pten³ has lethal \| late third instar larval stage phenotype, suppressible \| partially by wdb^GS9548/Scer\GAL4^arm.PS (Vereshchagina et al., 2008) Pten³ has hyperplasia \| somatic clone phenotype, suppressible by rictor^Δ2 (Hietakangas and Cohen, 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Pten³ has eye \| somatic clone phenotype, suppressible by rictor^Δ2 (Hietakangas and Cohen, 2007)
Additional Comments
Genetic Interactions
Statement Reference 77% of Pten[3]/Df(2L)flp170B animals survive to adulthood in the presence of Akt1[1]/+. Expression of Tsc1[Scer\UAS.cGa] and gig[Scer\UAS.cGa] under the control of Scer\GAL4[arm.PS] rescues lethality in 40% of Pten[3]/Df(2L)flp170B animals. Expression of wdb[GS9548] under the control of Scer\GAL4[arm.PS] rescues lethality in 52% of Pten[3]/Df(2L)flp170B animals. (Vereshchagina et al., 2008) rictor^Δ2 suppresses the overgrowth of the eye caused by homozygous Pten³ clones. (Hietakangas and Cohen, 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Pten¹ (Goberdhan et al., 1999)
Rescued by	Pten³ is rescued by Pten^+t6.3 (Goberdhan et al., 1999) Pten³ is rescued by Pten^hs.PG (Goberdhan et al., 1999)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	DPTEN³ (Goberdhan et al., 1999) Pten³ (Lee et al., 2010) PTEN³ (Hietakangas and Cohen, 2007, Pinal et al., 2006, Vereshchagina et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Lee et al., 2010, Science 327(5970): 1223--1228 Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. [FBrf0210161] Vereshchagina et al., 2008, J. Cell Sci. 121(20): 3383--3392 The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila. [FBrf0206002] Hietakangas and Cohen, 2007, Genes Dev. 21(6): 632--637 Re-evaluating AKT regulation: role of TOR complex 2 in tissue growth. [FBrf0201456] Pinal et al., 2006, Curr. Biol. 16(2): 140--149 Regulated and polarized PtdIns(3,4,5)P3 accumulation is essential for apical membrane morphogenesis in photoreceptor epithelial cells. [FBrf0190038] Coelho et al., 2005, Genetics 171(2): 597--614 A genetic screen for dominant modifiers of a small-wing phenotype in Drosophila melanogaster identifies proteins involved in splicing and translation. [FBrf0190712] Goberdhan et al., 1999, Genes Dev. 13(24): 3244--3258 Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway. [FBrf0123020]

Allele Dmel\Pten3

Allele Dmel\Pten³