Allele Dmel\Pten¹

General Information
Symbol	Dmel\Pten¹	Species	D. melanogaster
Name		FlyBase ID	FBal0104064
Feature type	allele	Associated gene	Dmel\Pten
Also Known As	DPTEN¹

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Goberdhan et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia \| somatic clone (Goberdhan et al., 1999) increased cell size \| recessive \| somatic clone (Bateman and McNeill, 2004) large body \| recessive \| somatic clone (Goberdhan and Wilson, 2002) lethal \| recessive (Goberdhan et al., 1999) planar polarity defective \| recessive \| somatic clone (Bateman and McNeill, 2004)
Phenotype Manifest In
	crossvein \| somatic clone (Goberdhan et al., 1999) eye \| somatic clone (Goberdhan et al., 1999, Goberdhan and Wilson, 2002) eye disc \| somatic clone (Bateman and McNeill, 2004) eye photoreceptor cell \| somatic clone (Pinal et al., 2006) interommatidial bristle \| somatic clone (Goberdhan et al., 1999) lipid particle & nurse cell \| somatic clone (Vereshchagina and Wilson, 2006) ommatidial precursor cluster \| somatic clone (Bateman and McNeill, 2004) ommatidium \| somatic clone (Goberdhan et al., 1999, Bateman and McNeill, 2004, Goberdhan and Wilson, 2002, Pinal et al., 2006) photoreceptor cell \| cell autonomous \| somatic clone (Goberdhan et al., 1999) rhabdomere \| cell autonomous \| somatic clone (Goberdhan et al., 1999) rhabdomere \| somatic clone (Pinal et al., 2006) wing & cell \| somatic clone (Goberdhan et al., 1999) wing \| somatic clone (Goberdhan et al., 1999) wing hair \| ectopic \| somatic clone (Goberdhan et al., 1999)
Detailed Description
	Statement Reference Pten[1] mutant somatic clones generated in the eye display highly disorganised ommatidia with compromised apical membranes. There is a failure of rhabdomere morphogenesis, and most photoreceptors display abnormally elongated, deformed, or split rhabdomeres. These defects are evident from mid-pupal development onwards. There is no evidence of photoreceptor degeneration in retinas from a series of aged adult flies. (Pinal et al., 2006) Nurse cells in 90% of Akt1¹ mutant clones show cell-autonomous formation of large aggregated droplets and a corresponding reduction in small perinuclear droplets compared to wild-type nurse cells. (Vereshchagina and Wilson, 2006) Mutant clones in the adult eye produce large cells and misrotated ommatidia. Cells in mutant clones in the eye disc show precocious differentiation several rows ahead of wild-type cells. In addition, ommatidia within clones in the eye disc initiate rotation before wild-type ommatidia. (Bateman and McNeill, 2004) Mosaic animals in which the eye is largely homozygous for Pten¹ (generated using the "eyFLP" system) have larger eyes than normal. The eyes contain about 12% more ommatidia than wild-type eyes and individual ommatidia are 78% larger in surface area. (Goberdhan and Wilson, 2002) Hemizygotes die as late embryos or early first instar larvae. Homozygous mutant clones in the eye bulge out from the eye surface. Ommatidia sometimes fuse interommatidial bristles are abnormal, particularly (but not exclusively) in the dorsal half of the eye. Mutant clones typically contain at least twice as many ommatidia as their wild type twin spots. The largest clones can form a hyperplastic tumor-like overgrowth, though this phenotype is less severe than for tumor suppressor mutations. Within each ommatidium cell type specification seems normal. Cell bodies of mutant photoreceptors are enlarged compared to wild type neighbors, and the rhabdomeres are misshapen. The cell size and rhabdomeric phenotypes are cell autonomous. Homozygous mutant clones in the wing reveal an increase in cell size. Wing hairs are occasionally duplicated and crossvein formation is abnormal. Occasional extra wing vein material develops. Longitudinal veins are rarely affected. (Goberdhan et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference Pten¹ has large body \| recessive \| somatic clone phenotype, non-suppressible by chico^flp147E/chico^flp147E (Goberdhan and Wilson, 2002)
NOT Suppressor of
Statement Reference Pten¹/Pten[+] is a non-suppressor of large body phenotype of Scer\GAL4^29BD, dally^{sec.Scer\UAS.T:Hsap\MYC} (Takeo et al., 2005)
Phenotype Manifest In
NOT suppressed by
Statement Reference Pten¹ has eye \| somatic clone phenotype, non-suppressible by chico^flp147E/chico^flp147E (Goberdhan and Wilson, 2002)
Suppressor of
Statement Reference Pten¹ is a suppressor \| partially of wing phenotype of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 (Goberdhan et al., 1999)
NOT Suppressor of
Statement Reference Pten¹/Pten[+] is a non-suppressor of pupa phenotype of Scer\GAL4^29BD, dally^{sec.Scer\UAS.T:Hsap\MYC} (Takeo et al., 2005) Pten¹/Pten[+] is a non-suppressor of wing phenotype of Scer\GAL4^29BD, dally^{sec.Scer\UAS.T:Hsap\MYC} (Takeo et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Pten¹/+; dally^{sec.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^29BD pupae show the same large pupae and disordered wing patterning phenotype as wild-type animals expressing dally^{sec.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^29BD. (Takeo et al., 2005) Mosaic animals in which the eye is largely homozygous for both Pten¹ and chico^flp147E (generated using the "eyFLP" system) have greatly enlarged eyes. The eyes contain about the same number of ommatidia as wild type, but the ommatidia are larger in surface area than normal. (Goberdhan and Wilson, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Pten³ (Goberdhan et al., 1999)
Rescued by	Pten¹ is rescued by Pten^{2.Scer\UAS.T:Avic\GFP}/Scer\GAL4^GMR.PF (Pinal et al., 2006) Pten¹ is rescued by Pten^+t6.3 (Goberdhan et al., 1999) Pten¹ is rescued by Pten^hs.PG (Goberdhan et al., 1999)
Partially rescued by	Pten¹ is partially rescued by Pten^{3.Scer\UAS.T:Avic\GFP}/Scer\GAL4^GMR.PF (Pinal et al., 2006)
Comments	Expression of Pten[2.Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[GMR.PF] is able to suppress all rhabdomeric phenotypes in Pten[1] mutant eyes. Expression of Pten[3.Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[GMR.PF] is able to suppress most of the mutant Pten[1] eye phenotype, but some split and misshapen rhabdomeres are still evident. (Pinal et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	DPTEN¹ (Lu et al., 2004, Goberdhan and Wilson, 2002, Goberdhan et al., 1999) Pten¹ (Vereshchagina and Wilson, 2006, ) pten¹ (Bateman and McNeill, 2004) PTEN¹ (Betschinger et al., 2006, Pinal et al., 2006, Vereshchagina et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Vereshchagina et al., 2008, J. Cell Sci. 121(20): 3383--3392 The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila. [FBrf0206002] Betschinger et al., 2006, Cell 124(6): 1241--1253 Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. [FBrf0189901] Pinal et al., 2006, Curr. Biol. 16(2): 140--149 Regulated and polarized PtdIns(3,4,5)P3 accumulation is essential for apical membrane morphogenesis in photoreceptor epithelial cells. [FBrf0190038] Vereshchagina and Wilson, 2006, Development 133(23): 4731--4735 Cytoplasmic activated protein kinase Akt regulates lipid-droplet accumulation in Drosophila nurse cells. [FBrf0193043] Takeo et al., 2005, Dev. Biol. 284(1): 204--218 Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. [FBrf0187400] Bateman and McNeill, 2004, Cell 119(1): 87--96 Temporal control of differentiation by the insulin receptor/tor pathway in Drosophila. [FBrf0180028] Lu et al., 2004, EMBO J. 23(5): 1089--1100 Localization of Tec29 to ring canals is mediated by Src64 and PtdIns(3,4,5)P(3)-dependent mechanisms. [FBrf0174599] Goberdhan and Wilson, 2002, Dev. Genes Evol. 212(4): 196--202 Insulin receptor-mediated organ overgrowth in Drosophila is not restricted by body size. [FBrf0148955] Goberdhan et al., 1999, Genes Dev. 13(24): 3244--3258 Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway. [FBrf0123020]

Allele Dmel\Pten1

Allele Dmel\Pten¹