The additional clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU enhances the supernumerary polar/stalk cell phenotype induced by AxnS044230 homozygous follicle cell clones.
The co-expression of Six4HM05254, does not suppress the increased number of basal stalk cells and the basal stalk defects induced by the expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood.
Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts and restores intermediate neural progenitor cells and ganglion mother cells in type II neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4wor.PA (analysed 72 hours after clone induction).
Type I neuroblast clones in the larval brain co-expressing kluScer\UAS.T:Ivir\HA1 and Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of 18 neuroblasts per clone and 100% of the clones show the supernumerary neuroblast phenotype.
Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts per clone as well as the frequency of the clones containing more than one neuroblast in type I neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4btl.PS in seqZ1241 mutant trachea is not sufficient to suppress the ganglionic branch bifurcation phenotype. Activation of N in tracheal cells does not inhibit migration of ganglionic branch cells and is not able to overcome the effects of higher FGF in the ganglionic branches surrounding tissues of seqZ1241 mutants.
The ability of ptcS2 to induce follicle stem cell duplications in clones and for the clones to take over the whole ovariole is suppressed by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub. The loss of follicle stem cell clones caused by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub is not substantially suppressed by ptcS2.
Nintra.GS.Scer\UAS; Scer\GAL4αTub84B.PL; wgl-17/wgl-17 somatic clones in the wing disc overproliferate, and non-autonomously induce overproliferation. However, unlike when such clones are wg+, this phenotype is mainly restricted to clones in the hinge and pleura, and is rarely seen in clones located in proximal regions of the wing pouch. Flow cytometry analysis of cell cycle progression in the mutant cells shows no difference in profile compared to wild-type cells.