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General Information
Symbol
Dmel\Nintra.GS.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0104072
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nintra, UAS-NICD, UAS-NotchIntra, UAS-Notchintra, Nintra, UAS-NICD, UAS-Nintra
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the intracellular domain of N.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU does not induce ectopic polar/stalk cells on the main body region in stage 4 egg chambers.

The expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood, leads to an increase in the number of basal stalk cells, associated with basal stalk defects, as compared to controls.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4ato.3.6 strongly reduces the number of medulla axons in the adult brain compared to wild type, while the initial outgrowth of these axons towards the medulla (at 32 hours after puparium formation) is not inhibited.

Type II neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4wor.PA contain mostly supernumerary neuroblasts and a few immature intermediate neural progenitor cells when analysed 72 hours after clone induction.

Type I neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of six neuroblasts per clone, although only 60% of the clones contain more than one neuroblast per clone.

Tracheal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4btl.PS prevents tracheal fusion, but does not arrest outgrowth or stall cells near the base of the ganglionic branch.

Expression of Nintra.GS.Scer\UAS in the eye disc under the control of Scer\GAL4Act5C.PP results in clones that overproliferate compared to wild type cells.

Expression of Nintra.GS.Scer\UAS using Scer\GAL4CG31670.R9D11 transforms Intermediate Neural Progenitors into ectopic type II neuroblasts in larval brains.

Follicle stem cell clones expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4tub, Scer\GAL4Act and Scer\GAL4e22c simultaneously are lost more rapidly from the ovarioles than control clones over time.

Follicle stem cell clones expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4tub are lost more rapidly from the ovarioles than control clones over time.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4C587 results in an increased number of cap cells in the female germarium. Extra cap cells are seen both at the normal location close to the terminal filament cells and also in ectopic locations away from the germarial tip. In germaria with increased numbers of cap cells in the normal location, there is also an increase in the number of spectrosome-containing single germ cells, which behave like germline stem cells (GSCs), at the germarial tip. The ectopically located cap cells appear to be functional as spectrosome-containing single germ cells, which behave as GSCs, are also closely associated with them. These ectopic GSCs anchor their spectrosome on the side that anchors the ectopic cap cell, as occurs in a normal GSC context. Some of the ectopic cap cells are surrounded by inner germarial sheath (IGS) cells or somatic follicle cells. Germ cells lying one cell away from the ectopic GSCs are often germline cysts (as they contain branched fusomes), indicating that the progeny of the ectopic GSCs can probably undergo normal differentiation. However, too many cap cells (more than seven) at either the normal or ectopic location often cause the accumulation of spectrosome-containing single germ cells (which resemble GSCs) located two or more cell diameters away.

When Nintra.GS.Scer\UAS is expressed under the control of Scer\GAL4Act5C.PI in inner germarial sheath cells of the adult ovary does not affect the differentiation status of the underlying germ cells.

Nintra.GS.Scer\UAS; Scer\GAL4fkh.14-3 causes ectopic cell movements in the developing proventriculus, but no obvious changes in endodermal or ectodermal cell fate.

Nintra.GS.Scer\UAS; Scer\GAL4dpp.blk1 results in overgrowth of the wing pouch and notum and an increase in BrdU incorporation in both of these regions. Similar results are seen in Nintra.GS.Scer\UAS; Scer\GAL4αTub84B.PL somatic clones. These clones also induce overgrowth non-autonomously. Flow cytometry analysis of cell cycle progression in the mutant cells shows no difference in profile compared to wild-type cells.

Nintra.GS.Scer\UAS; Scer\GAL4pros.PMG 30% of RP2 neurons are transformed into RP2 sib neurons, 99% of dMP2 neurons are converted to vMP2, 92% of hemi-segments exhibit an increase in U neurons and there is am overall decrease in EL neurons.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PI results in the formation of ectopic polar cells near the two poles of the egg chambers.

Embryos expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4arm.PS show loss of peripheral nervous system neurons and the central nervous system is reduced to irregular patches of tissue.

When Nintra.GS.Scer\UAS is driven by Scer\GAL4hs.PB and heatshocked between 8-12 hours APF no effect is seen on bristle pattern, but a high number of bristles lack bracts. Heat shock at earlier stages strongly reduces the number of bristles. Heatshock at later stages, causes many bristles to lack both shaft and bract.

Nintra.GS.Scer\UAS when driven by Scer\GAL4F442A.αTub84B.T:Hsim\VP16 suppresses neuroblast segregation in the embryo.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4ato.3.6 results in a severe inhibition of axonal branching over the lobula and a complete failure of innervation of the medulla.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The additional clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU enhances the supernumerary polar/stalk cell phenotype induced by AxnS044230 homozygous follicle cell clones.

The co-expression of Six4HM05254, does not suppress the increased number of basal stalk cells and the basal stalk defects induced by the expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood.

Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts and restores intermediate neural progenitor cells and ganglion mother cells in type II neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4wor.PA (analysed 72 hours after clone induction).

Type I neuroblast clones in the larval brain co-expressing kluScer\UAS.T:Ivir\HA1 and Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of 18 neuroblasts per clone and 100% of the clones show the supernumerary neuroblast phenotype.

Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts per clone as well as the frequency of the clones containing more than one neuroblast in type I neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4btl.PS in seqZ1241 mutant trachea is not sufficient to suppress the ganglionic branch bifurcation phenotype. Activation of N in tracheal cells does not inhibit migration of ganglionic branch cells and is not able to overcome the effects of higher FGF in the ganglionic branches surrounding tissues of seqZ1241 mutants.

A Vha55j2E9 mutant clone background does not suppress the overproliferation phenotype seen when Nintra.GS.Scer\UAS is expressed in eye disc clones under the control of Scer\GAL4Act5C.PP.

Coexpression of ermScer\UAS.T:Ivir\HA under the control of Scer\GAL4CG31670.R9D11 suppresses the ectopic neuroblasts induced by expression of Nintra.GS.Scer\UAS.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub has no effect on the maintenance of mam8 follicle stem cell clones.

The ability of ptcS2 to induce follicle stem cell duplications in clones and for the clones to take over the whole ovariole is suppressed by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub. The loss of follicle stem cell clones caused by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub is not substantially suppressed by ptcS2.

Nintra.GS.Scer\UAS; Scer\GAL4αTub84B.PL; wgl-17/wgl-17 somatic clones in the wing disc overproliferate, and non-autonomously induce overproliferation. However, unlike when such clones are wg+, this phenotype is mainly restricted to clones in the hinge and pleura, and is rarely seen in clones located in proximal regions of the wing pouch. Flow cytometry analysis of cell cycle progression in the mutant cells shows no difference in profile compared to wild-type cells.

The transformation of neurons (RP2 to RP2sib; dMP2 to vMP2; Usib to U) seen in Nintra.GS.Scer\UAS; Scer\GAL4pros.PMG embryos is unaffected by spdoG104/spdoG104.

In the absence of AP-2 activity, activated N is not able to rescue joint formation.

PsnC2 clones that also express Nintra.GS.Scer\UAS lead to double dorsal wing outgrowth.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Nintra.GS.Scer\UAS
Nintra.GS.UAS
Name Synonyms
Secondary FlyBase IDs
    References (36)