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General Information
Symbol
Hsap\HSPA1LUAS.cWa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Warrick
FlyBase ID
FBal0104117
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-HspA1L, UAS-hsp70, UAS-hspA1, Hsp70
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of an Hsap\HSPA1L cDNA.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Adults expressing Hsap\HSPA1LUAS.cWa under the control of Scer\GAL4elav.PLu do not exhibit climbing defects compared to controls.
Flies expressing Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PU do not exhibit any obvious eye phenotypes.
Expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PF has no effect on eye pigmentation.
Flies expressing Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4da.PU do not exhibit climbing defects.
Flies expressing Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4Mhc.PW show normal wing positions.
Expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PU does not cause degeneration or pigment loss in the adult eye.
Co-expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the mutant eye phenotype caused by expression of Hsap\HSPA1LK71E.Scer\UAS under the control of Scer\GAL4GMR.PF.
The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is not significantly affected by expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PF.
Expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.
The eye appears grossly normal in flies expressing Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4GMR.PF, although there are abnormalities in nuclear position and photoreceptor rhabdomere morphology.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Coexpression of the Hsap\HSPA1LScer\UAS.cWa transgene, results in efficient suppression of the wing posture phenotype of Scer\GAL4Mhc.PW>Hsap\PABPN117ala.Scer\UAS flies.
Xenogenetic Interactions
Statement
Reference
Co-expression of Hsap\HSPA1LScer\UAS.cWa fails to suppress the locomotor defects and reduced lifespan of flies expressing Hsap\APP2xAPP.SP.Scer\UAS under the control of Scer\GAL4elav.PU. Co-expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the increased cell death observed in adult Kenyon cells of the mushroom body, and enhances the increased size of the calyx at 1 day old; but expression of Hsap\HSPA1LScer\UAS.cWa fails to suppress the thin Kenyon cell axons and the decreased size of the calyx at 20 and 40 days old in flies expressing Hsap\APP2xAPP.SP.Scer\UAS under the control of Scer\GAL4ey-OK107. Co-expression of Hsap\HSPA1LScer\UAS.cWa fails to suppress the eye phenotypes of adult flies or the increased cell death observed in larval posterior eye discs in flies expressing Hsap\APP2xAPP.SP.Scer\UAS under the control of Scer\GAL4GMR.PU. Co-expression of Hsap\HSPA1LScer\UAS.cWa completely suppresses the eye phenotypes of flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU.
Expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4ey-OK107 partially suppresses the axon degeneration seen in bskflp147E mutant mushroom body neuron clones at 28 days. The number of enlarged and supernumerary axonal protrusions seen on bskflp147E mutant axon shafts is also significantly reduced. Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the mushroom body degeneration seen in 7 day old flies when NmnatJF03337 is expressed under the control of Scer\GAL4ey-OK107.
Co-expression of Hsap\HSPA1LScer\UAS.cWa strongly suppresses the eye defects caused by expression of Hsap\FUSScer\UAS.cMa.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF at 29[o]C. Co-expression of Hsap\HSPA1LScer\UAS.cWa strongly suppresses the decrease in lifespan caused by expression of Hsap\FUSScer\UAS.cMa.T:Ivir\HA1 under the control of Scer\GAL4elav.Switch.PO in the presence of RU486.
Co-expression of embNIG.13387R enhances the loss of rhabdomere phenotype caused by expression of Hsap\MJDfl.Q84.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF. This enhancement is suppressed by co-expression of Hsap\HSPA1LScer\UAS.cWa.
Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the eye pigmentation phenotype seen in 15 day old adults when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed under the control of Scer\GAL4D42 at 25[o]C. Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the eye pigmentation phenotype seen in 15 day old adults when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed under the control of Scer\GAL4D42 at 25[o]C.
Expression of Hsap\HSPA1LScer\UAS.cWa suppresses the climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU. Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the wing posture and climbing defects seen when Disc\RFPCAG250.DsRed2.Scer\UAS is expressed under the control of Scer\GAL4da.PU.
30 day old Maur\PrpScer\UAS.cFa Scer\GAL4da.G32 fly brains co-expressing Hsap\HSPA1LScer\UAS.cWa exhibit very few vacuolated cells and normal nuclei. 40 day old Maur\PrpScer\UAS.cFa Scer\GAL4ey-OK107 fly brains co-expressing Hsap\HSPA1LScer\UAS.cWa demonstrate robust protection of mushroom body projections. Co-expression of Hsap\HSPA1LScer\UAS.cWa reduces the decline in climbing ability seen in Maur\PrpScer\UAS.cFa Scer\GAL4Act.PU flies. Co-expression of Hsap\HSPA1LScer\UAS.cWa suppresses the reduced distribution and speed seen in 20 day old Maur\PrpScer\UAS.cFa Scer\GAL4Act.PU flies when tracked in a custom arena.
Expression of Hsap\HSPA1LScer\UAS.cWa suppresses the pigment cell degeneration seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU. Expression of Hsap\HSPA1LScer\UAS.cWa significantly delays the progressive photoreceptor degeneration seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155. Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the reduction in life expectancy seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of any of Scer\GAL4elav-C155, Scer\GAL4repo.PU or Scer\GAL4Eaat1.PR. Expression of Hsap\HSPA1LScer\UAS.cWa suppresses the γ-lobe kenyon cell degeneration seen in the mushroom bodies when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155. Expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the locomotor defects seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of either Scer\GAL4elav-C155 or Scer\GAL4repo.PU.
The lethality of trpml1 homozygotes and the locomotor defects of trpml1 homozygous adult escapers is suppressed by expression of Hsap\HSPA1LScer\UAS.cWa under the control of either Scer\GAL4Act5C.PI or Scer\GAL4elav-C155.
Co-expression of Hsap\HSPA1LScer\UAS.cWa can suppress neurodegeneration induced by Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1.
The progressive degeneration and pigmentation loss eye phenotype characteristic for flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is suppressed by co-expression of Hsap\HSPA1LScer\UAS.cWa although signs of degeneration are eventually observed in aged (32 days post-eclosion) flies.
The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Hsap\HSPA1LScer\UAS.cWa.
The loss of dorsomedial dopaminergic neurons seen in 20 day old flies expressing Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL is suppressed by co-expression of Hsap\HSPA1LScer\UAS.cWa, although there is no change in the number, morphology or distribution of inclusions seen in the dopaminergic neurons.
Co-expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the eye degeneration seen in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF. Co-expression of both Hsap\HSPA1LScer\UAS.cWa and DnaJ-1Scer\UAS.T:Zzzz\FLAG completely suppresses the eye degeneration seen in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.
Co-expression of Hsap\HSPA1LScer\UAS.cWa with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF suppresses the external and internal eye phenotypes associated with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1. Co-expression of Uba2C175S.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (all under the control of Scer\GAL4GMR.PF) fails to suppress the enhancement of the eye degeneration phenotype found in Uba2C175S.Scer\UAS ; Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 flies. Co-expression of Pros261.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) suppress the eye phenotype.
Expression of Hsap\HSPA1LScer\UAS.cWa under the control of Scer\GAL4elav-C155 restores eye structure in 10 day old flies expressing Hsap\HTTGMR.Q120, such that photoreceptor neurons appear normal. Flies that co-express Hsap\HSPA1LScer\UAS.cWa, Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 and DnaJ-1ΔJ.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU have normal eyes. Co-expression of Hsap\HSPA1LScer\UAS.cWa partially suppresses the retinal degeneration seen in flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU. Simultaneous co-expression of both DnaJ-1Scer\UAS.T:Zzzz\FLAG and Hsap\HSPA1LScer\UAS.cWa results in a stronger suppression of the retinal degeneration caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU than expression of either construct alone.
Suppresses the eye phenotype caused by expression of Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF. Does not modify the disrupted eye phenotype caused by expression of eyScer\UAS.cHa or Rnor\MAP2Scer\UAS.cAa under the control of Scer\GAL4unspecified.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\HSPA1LScer\UAS.cWa
Hsap\HSPA1LUAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Warrick
Secondary FlyBase IDs
    References (32)