FlyBase Allele Report: Dmel\rpr[UAS.cIa]

General Information

Symbol

Dmel\rpr^UAS.cIa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Igaki

FlyBase ID

FBal0104433

Feature type

allele

Associated gene

Dmel\rpr

Associated Insertion(s)

Carried in Construct

P{UAS-rpr.I}

Also Known As

UAS-rpr

Key Links

Transgenic product class

cDNA

(Igaki et al., 2000)

wild_type

(Igaki et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Igaki et al., 2000)

wild_type

(Igaki et al., 2000)

Mutagen

in vitro construct

(Igaki et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-rpr.I}

Cytology

Description

UASt sequences drive the rpr cDNA.

(Igaki et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

rpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^Act5C.PHb

(Igaki et al., 2002, Kuranaga et al., 2002)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Oshima, 2006)

eye, with Scer\GAL4^GMR.PF

(Kuranaga et al., 2002)

macrochaeta & mesothoracic tergum, with Scer\GAL4^sca-537.4

(Igaki et al., 2002)

macrochaeta & scutellum, with Scer\GAL4^sca-537.4

(Igaki et al., 2002)

macrochaeta & tergum, with Scer\GAL4^sca-537.4

(Igaki et al., 2002)

wing, with Scer\GAL4^vg.PM

(Igaki et al., 2002)

Detailed Description

Statement

Reference

Expression of rpr^Scer\UAS.cIa under the control of Scer\GAL4^slbo.2.6 causes border cells to stall at the anterior end of the egg chamber. The stalled border cells have a condensed morphology that is typical of apoptotic cells.

(Oshima, 2006)

When rpr^Scer\UAS.cIa is driven by Scer\GAL4^vg.PM, regional wing defects are seen. When rpr^Scer\UAS.cIa is driven by Scer\GAL4^sca-537.4, complete loss of bristles are seen in the tergum, notum and scutellum.

(Igaki et al., 2002)

Expression of rpr^Scer\UAS.cIa under the control of Scer\GAL4^Act5C.PHb in S2 cells results in cell death.

(Igaki et al., 2002)

rpr^Scer\UAS.cIa; Scer\GAL4^GMR.PF flies have extremely reduced eyes, or lack them altogether. Transfection of S2 cells with rpr^Scer\UAS.cIa and Scer\GAL4^Act5C.PHb causes cell death.

(Kuranaga et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PHb, rpr^UAS.cIa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PHb

(Igaki et al., 2002)

Scer\GAL4^Act5C.PHb, rpr^UAS.cIa has increased cell death phenotype, suppressible | partially by Diap1^UAS.Tag:FLAG, Scer\GAL4^Act5C.PHb

(Kuranaga et al., 2002)

Other

Statement

Reference

Scer\GAL4^btl.PS, hid^UAS.cIa, rpr^UAS.cIa has increased cell death phenotype

(Oshima et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Igaki et al., 2002)

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, suppressible by Dronc^C318G.UAS, Scer\GAL4^GMR.PF

(Igaki et al., 2002)

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Igaki et al., 2002)

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, suppressible | partially by Ask1^K618M.UAS, Scer\GAL4^GMR.PF

(Kuranaga et al., 2002)

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^GMR.PF

(Kuranaga et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rpr^UAS.cIa has eye phenotype, non-suppressible by p38b^DN.UAS/Scer\GAL4^GMR.PF

(Kuranaga et al., 2002)

Other

Statement

Reference

Scer\GAL4^btl.PS, hid^UAS.cIa, rpr^UAS.cIa has presumptive embryonic/larval tracheal system phenotype

(Oshima et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of rpr^Scer\UAS.cIa and W^Scer\UAS.cIa, under the control of Scer\GAL4^btl.PS, disrupts the tracheal system and increases the number of apoptotic cells.

(Oshima et al., 2006)

The cell death seen in S2 cells expressing rpr^Scer\UAS.cIa under the control of Scer\GAL4^Act5C.PHb is suppressed by coexpression of BacA\p35^Scer\UAS.cHa.

(Igaki et al., 2002)

The eye phenotype of rpr^Scer\UAS.cIa; Scer\GAL4^GMR.PF flies is partially suppressed by Pk92B^{K618M.Scer\UAS} or bsk^DN.Scer\UAS, but not by p38b^DN.Scer\UAS. Increased cell death due to transfection of S2 cells with rpr^Scer\UAS.cIa and Scer\GAL4^Act5C.PHb is partially suppressed by th^{Scer\UAS.T:Zzzz\FLAG}.

(Kuranaga et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pUAS-rpr" and transfected into S2 cells to study its effect on debcl induced apoptosis.

(Igaki et al., 2000)

Carried in plasmid "pUAS-rpr" and transfected into S2 cells.

(Srinivasula et al., 2002)

Carried in plasmid "pUAS-reaper" and transfected into S2 cells.

(Igaki et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

rpr^Scer\UAS.cIa

rpr^UAS.cIa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Igaki

(Igaki et al., 2000)

Secondary FlyBase IDs

References (8)

Report Sections

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