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General Information
Symbol
Dmel\nkdUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zeng
FlyBase ID
FBal0104471
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nkd
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive nkd expression.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies overexpressing nkdScer\UAS.cZa driven by Scer\GAL4ap-md544 show wings that are reduced in size and blistered.

Expression of nkdScer\UAS.cZa under the control of Scer\GAL4B119 results in near total loss of sternite bristles with occasional residual laterally displaced bristles.

A duplicated NB 7-3 is formed in 40% of hemisegments in embryos expressing nkdScer\UAS.cZa under the control of Scer\GAL4en-e16E.

Expression of nkdScer\UAS.cZa under the control of Scer\GAL4l(2)E105-E105 results in variable truncation of the legs. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4B119 results in variable duplication of the legs. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4unspecified results in a number of phenotypes including wing to notum transformations, leg truncations and duplications, loss, lateral displacement and disorientation of sternite bristles, haltere loss, ventral eye reduction, loss of wing margin, extra wing anterior crossveins and loss of antennae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of nkdScer\UAS.cZa driven by Scer\GAL4arm.PS is unable to block the cell-fate transformation induced by armS10.Scer\UAS.T:Hsap\MYC expression.

Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c in a wgl-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50CAR2; the triple mutant embryos have a wild-type cuticle pattern. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c significantly rescues the cuticle pattern defects seen in RacGAP50CAR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50CAR2 embryos is unaffected if the embryos are also expressing nkdScer\UAS.cZa under the control of Scer\GAL4e22c.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The naked cuticle phenotype of nkd3 embryos is partially rescued by expression of nkdScer\UAS.cZa under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4en-e16E, with more complete rescue being seen when Scer\GAL4ptc-559.1 is used as the driver.

Expression of nkdScer\UAS.cZa under the control of Scer\GAL4da.G32 rescues the cuticle defects of nkd2/nkd3 embryos.

Rescues the cuticle defects of nkd2/nkd3 embryos when expressed under the control of Scer\GAL4da.G32.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
nkdScer\UAS.cZa
nkdUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zeng
Secondary FlyBase IDs
    References (10)