Allele Dmel\nkd^Scer\UAS.cZa

General Information
Symbol	Dmel\nkd^Scer\UAS.cZa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Zeng	FlyBase ID	FBal0104471
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\nkd

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Zeng et al., 2000)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive nkd expression. (Zeng et al., 2000)
Assay mode	In transgenic Drosophila (intraspecific) (Zeng et al., 2000)
Carried in construct	P{UAS-nkd.Z} (Rousset et al., 2002, Deshpande et al., 2001, Jones, 2005, Zeng et al., 2000, Axelrod, 2001, Rousset et al., 2001, Brennecke et al., 2003, Waldrop et al., 2006, Zhang et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type, with Scer\GAL4^GMR.PF (Rousset et al., 2001) visible, with Scer\GAL4^B119 (Rousset et al., 2002, Zeng et al., 2000) visible, with Scer\GAL4^{l(2)E105-E105} (Zeng et al., 2000) tissue polarity defective, with Scer\GAL4^hs.PB (Rousset et al., 2001)
Phenotype Manifest In
	abdominal sternite bristle, with Scer\GAL4^B119 (Rousset et al., 2002) leg, with Scer\GAL4^B119 (Zeng et al., 2000) leg \| ectopic, with Scer\GAL4^B119 (Zeng et al., 2000) neuroblast NB7-3 \| ectopic, with Scer\GAL4^en-e16E (Deshpande et al., 2001) wing, with Scer\GAL4^hs.PB (Rousset et al., 2001) wing hair, with Scer\GAL4^hs.PB (Rousset et al., 2001) wing, with Scer\GAL4^unspecified (Zeng et al., 2000) scutum \| ectopic, with Scer\GAL4^unspecified (Zeng et al., 2000) leg, with Scer\GAL4^unspecified (Zeng et al., 2000) leg \| ectopic, with Scer\GAL4^unspecified (Zeng et al., 2000) abdominal sternite bristle, with Scer\GAL4^unspecified (Zeng et al., 2000) haltere, with Scer\GAL4^unspecified (Zeng et al., 2000) eye \| ventral, with Scer\GAL4^unspecified (Zeng et al., 2000) wing margin bristle, with Scer\GAL4^unspecified (Zeng et al., 2000) anterior crossvein \| ectopic, with Scer\GAL4^unspecified (Zeng et al., 2000) antenna, with Scer\GAL4^unspecified (Zeng et al., 2000) leg, with Scer\GAL4^{l(2)E105-E105} (Zeng et al., 2000)
Detailed Description
	Statement Reference Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^{l(2)E105-E105} results in variable truncation of the legs. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^B119 results in variable duplication of the legs. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^unspecified results in a number of phenotypes including wing to notum transformations, leg truncations and duplications, loss, lateral displacement and disorientation of sternite bristles, haltere loss, ventral eye reduction, loss of wing margin, extra wing anterior crossveins and loss of antennae. (Zeng et al., 2000) nkd^Scer\UAS.cZa when driven by Scer\GAL4^GMR.PF has no visible effect on eye development. Expression of nkd^Scer\UAS.cZa when driven by Scer\GAL4^hs.PB 24 h after puparium formation (APF) produces adult flies with wing hair polarity defects that are indistinguishable from those seen in dsh¹ mutant adults. (Rousset et al., 2001) A duplicated NB 7-3 is formed in 40% of hemisegments in embryos expressing nkd^Scer\UAS.cZa under the control of Scer\GAL4^en-e16E. (Deshpande et al., 2001) Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^B119 results in near total loss of sternite bristles with occasional residual laterally displaced bristles. (Rousset et al., 2002)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by porⁱ⁸ (Zeng et al., 2000) Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by wg^l-12 (Zeng et al., 2000) Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by wg^l-17 (Zeng et al., 2000) Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by dsh⁴⁷⁷ (Zeng et al., 2000) Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by dsh³ (Zeng et al., 2000) Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, enhanceable by arm⁴ (Zeng et al., 2000)
Suppressed by
Statement Reference Scer\GAL4^unspecified, nkd^Scer\UAS.cZa has visible phenotype, suppressible by sgg^M11 (Zeng et al., 2000)
Suppressor of
Statement Reference Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, dsh^Scer\UAS.cAa (Rousset et al., 2001, Waldrop et al., 2006) nkd^Scer\UAS.cZa/Scer\GAL4^hs.PB is a suppressor of tissue polarity defective phenotype of fz^hs.PP (Rousset et al., 2001)
NOT Suppressor of
Statement Reference Scer\GAL4^e22c/nkd^Scer\UAS.cZa is a non-suppressor of lethal \| embryonic stage \| recessive phenotype of tum^AR2 (Jones, 2005) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, dsh^{ΔbPDZ.Scer\UAS} (Waldrop et al., 2006)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^e22c/nkd^Scer\UAS.cZa is a suppressor \| partially of denticle phenotype of tum^AR2 (Jones, 2005) Scer\GAL4^e22c/nkd^Scer\UAS.cZa is a suppressor \| partially of embryonic/first instar larval cuticle phenotype of tum^AR2 (Jones, 2005) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, dsh^Scer\UAS.cAa (Rousset et al., 2001, Waldrop et al., 2006) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, dsh^Scer\UAS.cAa (Rousset et al., 2001) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a suppressor of interommatidial bristle phenotype of wg^sev.PC (Rousset et al., 2001) nkd^Scer\UAS.cZa/Scer\GAL4^hs.PB is a suppressor of wing phenotype of fz^hs.PP (Rousset et al., 2001) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, Xlae\csnk1e^KD.Scer\UAS (Zhang et al., 2006)
NOT Suppressor of
Statement Reference Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a non-suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, arm^{S10.Scer\UAS.T:Hsap\MYC} (Rousset et al., 2001) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a non-suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, arm^{S10.Scer\UAS.T:Hsap\MYC} (Rousset et al., 2001) Scer\GAL4^e22c/nkd^Scer\UAS.cZa is a non-suppressor of embryonic epidermis & nucleus phenotype of tum^AR2 (Jones, 2005) Scer\GAL4^GMR.PF/nkd^Scer\UAS.cZa is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, dsh^{ΔbPDZ.Scer\UAS} (Waldrop et al., 2006)
Other
Statement Reference Scer\GAL4^e22c, nkd^Scer\UAS.cZa, wg[+]/wg^l-17 has embryonic/first instar larval cuticle phenotype (Jones, 2005) Scer\GAL4^e22c, nkd^Scer\UAS.cZa, wg^l-17 has embryonic/first instar larval cuticle phenotype (Jones, 2005)
Additional Comments
Genetic Interactions
Statement Reference Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c in a wg^l-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50C^AR2; the triple mutant embryos have a wild-type cuticle pattern. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c significantly rescues the cuticle pattern defects seen in RacGAP50C^AR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50C^AR2 embryos is unaffected if the embryos are also expressing nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c. (Jones, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^da.G32/nkd^Scer\UAS.cZa rescues nkd³/nkd² (Rousset et al., 2002, Waldrop et al., 2006)
Comments	Expression of nkd[Scer\UAS.cZa] under the control of Scer\GAL4[da.G32] rescues the cuticle defects of nkd[2]/nkd[3] embryos. (Waldrop et al., 2006) Rescues the cuticle defects of nkd²/nkd³ embryos when expressed under the control of Scer\GAL4^da.G32. (Rousset et al., 2002)
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	nkd^Scer\UAS.cZa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Zeng (Zeng et al., 2000)
Secondary FlyBase IDs

References ( 9 )
Research paper	Waldrop et al., 2006, Genetics 174(1): 331--348 An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist naked cuticle. [FBrf0192436] Zhang et al., 2006, Dev. Biol. 299(1): 221--237 Regulation of wingless signaling by the CKI family in Drosophila limb development. [FBrf0193614] Jones, 2005, Genetics 169(4): 2075--2086 RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. [FBrf0188508] Brennecke et al., 2003, Cell 113(1): 25--36 bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. [FBrf0158764] Rousset et al., 2002, J. Biol. Chem. 277(50): 49019--49026 Zinc-dependent interaction between dishevelled and the Drosophila Wnt antagonist naked cuticle. [FBrf0155851] Axelrod, 2001, Genes Dev. 15(10): 1182--1187 Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. [FBrf0135774] Deshpande et al., 2001, Development 128(17): 3253--3261 Successive specification of Drosophila neuroblasts NB 6-4 and NB 7-3 depends on interaction of the segment polarity genes wingless, gooseberry and naked cuticle. [FBrf0138370] Rousset et al., 2001, Genes Dev. 15(6): 658--671 naked cuticle targets dishevelled to antagonize Wnt signal transduction. [FBrf0135776] Zeng et al., 2000, Nature 403(6771): 789--795 naked cuticle encodes an inducible antagonist of Wnt signalling. [FBrf0125452]

Allele Dmel\nkdScer\UAS.cZa

Allele Dmel\nkd^Scer\UAS.cZa