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General Information
Symbol
Dmel\mei-P26mfs1
Species
D. melanogaster
Name
FlyBase ID
FBal0104481
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of the ends of the P{lacW} element and 0.7-1.6kb of DNA flanking both sides of the original insertion site. There may also be a more complex rearrangement, possibly involving the insertion of sequence. Some P{lacW} sequence may remain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Testes of homozygous males have cysts full of many small cells, which appear similar to early germ cells seen at the apical tip of the wild-type testis. EdU incorporation assays show that early germ cells in the mutant testis undergo several extra rounds of mitotic division and behave as transit amplifying cells rather than as stem cells, with all cells within a cyst undergoing S phase synchronously. The overproliferating cysts in the mutant testes eventually degenerate. The nucleoli of mutant early germ cells are larger than normal. 32% of germline cysts do switch to the spermatocyte state, but only a third of these contain the normal number of 16 spermatocytes per cyst and the differentiating cells show a number of gross morphological defects.

Germaria are filled with individual spectrosome-containing cells and cysts containing varying numbers of cells connected by a fusome in mutant ovaries. Nurse cells and oocytes are not formed and fusomes or spectrosomes are maintained at later stages of oogenesis.

Cell diameters are increased compared to wild type and no longer decrease as cells are displaced from the stem cell niche in mei-P26fs1/mei-P26mfs1 ovaries.

Homozygous ovaries are completely tumourous and no normal looking egg chambers are seen. Homozygous females do not lay eggs. Testes of hemizygous males do not contain any mature motile spermatozoa, although somewhat disorganised immature elongated spermatid bundles are produced. In addition, the testes contain highly refractile cysts that most likely represent degenerating cysts of spermatogonial cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

mei-P26mfs1 has nucleolus phenotype, suppressible by bam+t2.9

mei-P26mfs1 has nucleolus phenotype, suppressible by bamΔPEST

Additional Comments
Genetic Interactions
Statement
Reference

Expression of bam+t2.9 completely rescues the overproliferation defects seen in the testes of mei-P26mfs1 males and 72% of the cysts in the spermatocyte region have 16 spermatocytes per cyst. The enlarged nucleolus phenotype seen in mei-P26mfs1 testes is also suppressed. The spermatocyte and spermatid differentiation defects are not rescued: 27% of cysts from the spermatocyte region have differentiation defects in the rescued males.

Expression of bamΔPEST completely rescues the overproliferation defects seen in the testes of mei-P26mfs1 males and 43% of the cysts in the spermatocyte region have 16 spermatocytes per cyst. The enlarged nucleolus phenotype seen in mei-P26mfs1 testes is also suppressed. The spermatocyte and spermatid differentiation defects are not rescued: 30% of cysts from the spermatocyte region have differentiation defects in the rescued males.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments

The overproliferating spermatogonial cysts and spermatocyte differentiation defects seen in the testes of mei-P26mfs1 males are rescued by Dp(1;4)A17 and by mei-P26+t13.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)