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General Information
Symbol
Dmel\flw6
Species
D. melanogaster
Name
FlyBase ID
FBal0104623
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

A10406698T

Reported nucleotide change:

A?T

Amino acid change:

Y264F | flw-PA; Y133F | flw-PB; Y133F | flw-PC; Y133F | flw-PD

Reported amino acid change:

Y133F

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Y133F. Nucleotide substitution: TAC?TTC

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

flw6/Y flies have a survival rate of less than 1%.

flw6 homozygous mutant egg chambers generate an irregular wavy egg chamber epithelium. Mutant egg chambers are not round or ellipsoid like in wild-type but are either stretched or develop bulges.

On the rare occasion that a flw6 male fly survives to adulthood, it has severely crumpled wings. Egg chambers that are flw6 mutant within adult flies can be generated by expressing flwScer\UAS.cRa under the control of Scer\GAL4arm.PS in somatic cells, so that somatic cells express wild-type flw but the female germline does not. The flw6 homozygous egg chambers have disrupted actin cytoskeletons and abnormal nuclear structures that indicate a failure of the transfer of nurse cell contents into the oocyte at stages 10B and 11. Additionally, the ring canals, which join nurse cells to each other and to the oocyte, fail to grow properly and are therefore smaller than in wild type.

flw1/flw6 flies are completely flightless. Indirect flight muscles (IFMs) are never present in flw1/flw6 flies, but the jump muscle (tergal depressor of trochanter muscle) appears normal. Less than 1% of flw6/Y males survive to adulthood at 25oC. The period of lethality extends from the second larval instar onwards. Dying larvae are very sluggish. The muscle pattern in these larvae is clearly disrupted, many muscles are missing or weakly attached. In the few flw6/Y males that do eclose exhibit poor ambulatory ability, IFMs are absent and the tergal depressor of trochanter muscle (TDT) disorganised. Wings are crumpled and/or blistered.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

flw6 has lethal phenotype, suppressible | partially by stckwt.Tag:FLAG

flw6 has lethal | recessive phenotype, suppressible | partially by bsk1/bsk[+]

flw6 has lethal | recessive phenotype, suppressible | partially by bsk2

flw6 has lethal | male phenotype, suppressible by Tm1Su(flw)4

flw6 has lethal | male phenotype, suppressible by sqhA20.Tag:FLAG

flw6 has lethal | male phenotype, suppressible by sqhA21.Tag:FLAG

flw6 has lethal | male phenotype, suppressible by zip02957

flw6 has lethal | male phenotype, suppressible by zipIIX62

flw6 has lethal | male phenotype, suppressible by zipSu(flw)1

flw6 has lethal | male phenotype, suppressible by zipSu(flw)2

flw6 has lethal | male phenotype, suppressible by zipSu(flw)3

flw6 has lethal | male phenotype, suppressible by zip1

flw6 has lethal | male phenotype, suppressible by MbsL.hs

flw6 has lethal | male phenotype, suppressible by Rho1E3.10

flw6 has lethal | male phenotype, suppressible by RhoGEF204291

flw6 has lethal | male phenotype, suppressible by Tm102299

NOT suppressed by
Enhancer of
Phenotype Manifest In
Suppressed by
Statement
Reference

flw6 has wing phenotype, suppressible by Tm1Su(flw)4

flw6 has wing phenotype, suppressible by zipSu(flw)1

flw6 has wing phenotype, suppressible by zipSu(flw)2

flw6 has wing phenotype, suppressible by zipSu(flw)3

NOT suppressed by
Statement
Reference

flw6 has phenotype, non-suppressible by Mlc2S66A,S67A

Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

flw6/flw6 or flw6 hemizygosity enhances the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crb11A22 background.

stckwt.T:Zzzz\FLAG increases the frequency of male flw6 escapers from 2.4% to 21.3% at 18[o]C. The rescued males are fertile.

The viability of flw6 mutants is much greater in a sqhA21.T:Zzzz\FLAG background than a sqhA20.T:Zzzz\FLAG background. The crumpled wing phenotype of flw6 flies is partially rescued by zipSu(flw)1, zipSu(flw)2 and zipSu(flw)3 and fully rescued by Tm1Su(flw)4.

The addition of Mlc2S66A,S67A does not suppress flw mutant phenotypes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Over 80% of males survive when flwScer\UAS.cRa is expressed, under the control of Scer\GAL4arm.PS, in a flw6 background. The transgene also rescues the crumpled wing phenotype in surviving flw6 males.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (8)