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General Information
Symbol
Zzzz\CAG127Q.UAS.Tag:HA
Species
a. artificial
Name
FlyBase ID
FBal0104696
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-127Q, UAS-Q127, UAS-poly127Q, 127Q
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a 127 amino acid polyglutamine tract (derived from the pros gene) tagged with Tag:HA. The polyglutamine tract is flanked by 8 amino acids (MGGPPSTP) on the N-terminal side and 13 amino acids on the C-terminal side (TSRTYPYDVPDYA).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by nejP
is ameliorated by nejEP1149
is ameliorated by nejEP1179
is exacerbated by Khc9
is ameliorated by ZwUAS.cLa
is ameliorated by DnaJ-1UAS.cKb
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c42 results in lethality in P-stage. Progeny appear grossly normal in both morphology and behavior until pupariation. Pupae are smaller in size as compared to wild type and display unevenly pigmented pupal cases, defective arrangement and morphology of trachea; begin to show signs of desiccation and shrinkage by 24-72h APF; display defects in epithelial morphogenesis, including dorsal closure and head eversion defects, leading to micro- or cryptocephaly; rudimentary or absent thoraces, and loss of 1-2 abdominal segments; defects in Malpighian tubules (MTs) including ectopic white concretions at 96h APF, loss of distinction between different segments, and variable reduction in lumen size or lumen loss of the main MT segment. MT function is severely impaired in both pupal and 3rd instar larval stages. Principal cells of the MT display enlarged nuclei with loose or fragmented chromatin in both third instar larvae and at 96h APF.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c649 results in lethality in P-stage. A small fraction are able to develop until just before eclosion, but are unable to open the pupal case and subsequently die; these flies display abnormal haltere position, abnormally large legs, rudimentary wings that appear to adhere to the abdomen, and excessive cuticle pigmentation. With assisted eclosion, these flies survive only up to 2-3 hrs.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Uro.PT does not result in lethality.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU results in flies with severely abnormal eyes with an absence of pigmentation. Complete photoreceptor loss is seen in the retina.

Animals expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU show variable eye degeneration with the phenotypes including loss of pigmentation, formation of necrotic patches and collapsed eyes. Loss of ommatidial clusters and ommatidial fusions are seen.

Polyglutamine inclusion bodies and disruption of the rhabdomere arrangement is seen in the eye discs of third instar larvae expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU. The axonal projections of photoreceptor axons into the optic lobe are disrupted. The organisation of actin filaments in the eye disc is disrupted.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in a significant (35%) reduction in hatching rate of embryos compared to controls and most embryos are deformed.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF results in eye degeneration.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF results in rough eyes which show loss of pigmentation.

The eye-degeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is not mitigated by feeding flies with linoleic acid and linolenic acid.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the developing eye under the control of Scer\GAL4GMR.PF induces clear defects in the eye, with defective ommatidia seen in 18% of cases.

Scer\GAL4GMR.PU-driven expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 induces severe eye degeneration and results in glazed, de-pigmented and collapsed eyes. Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 expressing pupal eyes show severe disorganisation and in many cases a complete loss of photoreceptor cells is also seen.

Unlike wild-type controls, flies overexpressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU do not show any significant phototaxis.

Pan-neuronal overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4elav.PU results in 100% lethality at pupal stage.

Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 causes eye degeneration.

Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 produces poly(Q) inclusion bodies in eye disc cells.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 using Scer\GAL4GMR.PF results in collapsed eyes with loss of pigmentation and disruption of the regular array of ommatidia. Retinal structure is disrupted, photoreceptor neurons are lost, and large vacuoles appear in the tissue.

2-day old flies expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 using Scer\GAL4GMR.PF fail to detect light.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF results in a glassy external eye phenotype with severe ommatidial disorganisation. The flies show severe retinal degeneration with abundant vacuolisation.

Retinas appear disorganised in 5 week old flies expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ninaE.PD.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4C5, does not result in wing abnormalities.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the developing eye disc, under the control of Scer\GAL4GMR.PF results in a loss of pigmentation and disruption of the regular ordered arrays of ommatidia in adult eyes without affecting the eye size. Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the developing eye disc, under the control of Scer\GAL4GMR.PF leads to disarrayed axonal projections in the optic stalk.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PB results in a rough eye phenotype which can be partially rescued by AR-A014418.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4GMR.PF results in a severe eye phenotype, however, while Scer\GAL4GMR.PF drives expression in the wing, no wing vein phenotype is observed. Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4C5 in the wing pouch does not cause significant vein abnormalities, although it does induce a mild wing atrophy at 29oC. Overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4C96 in the wing margin does not cause patterning defects.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF results in a retinal degeneration phenotype.

Animals expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4179Y or Scer\GAL4Appl.G1a exhibit a severe sluggish larval movement phenotype. A large increase in neuronal apoptosis is seen. When Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is overexpressed at 29oC larvae do not survive to adulthood but die at second or third instar larval stage.

Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PF causes progressive eye degeneration. Mutants are effectively blind, exhibiting defective phototaxis behaviour.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the developing eye under the control of Scer\GAL4GMR.PF results in severely collapsed eyes that lack pigmentation. An accumulation of poly(Q) clumps is detected in mutant eyes.

Expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the developing nervous system under the control of Scer\GAL4Appl.G1a results in pre-adult lethality.

Transgenic flies carrying Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and Scer\GAL4GMR.PY have a rough eye phenotype.

Adults expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF have severely abnormal eyes and lack pigmentation. The retina is badly deformed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
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Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
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NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Df(3R)Hsp70A and Df(3R)Hsp70B do not enhance the eye degeneration phenotype seen in flies expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU.

Expression of DnaJ-1Scer\UAS.cKb suppresses the eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Young animals have spotted pigment loss in the retina, indicating the degeneration of retinal cells, and this phenotype is more visible in aged animals. The photoreceptor loss is partially suppressed; ommatidia are observed that have small and missing rhabdomeres. The phenotype is still suppressed in a Df(3R)Hsp70A Df(3R)Hsp70B mutant background.

Expression of Hsp60Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsp60Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsp68Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsp68Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-1Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-1Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-2Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-2Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-3Scer\UAS.cEa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-3Scer\UAS.cEa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-4Scer\UAS.cEa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-4Scer\UAS.cEa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-5Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70-5Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsp83Scer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsp83Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70CbScer\UAS.cKa does not suppress the external eye degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. The retinal degeneration is also not suppressed.

Expression of Hsc70CbScer\UAS.cKa suppresses the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU. No pigmentation loss is seen in either young or aged animals and at one day old all seven photoreceptors with normal sized rhabdomeres are detected.

In 10 day old animals, severe neuronal degeneration marked by vesicle accumulation, loss of rhabdomeres, and invasion of pigment granules is detected in flies expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb under the control of Scer\GAL4GMR.PU.

Expression of Hsc70CbK68S.Scer\UAS does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70CbKD.Scer\UAS does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and DnaJ-1Scer\UAS.cKb are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70CbScer\UAS.cKa does not further suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and mrjScer\UAS.T:Zzzz\FLAG are co-expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsc70CbHMS01080 enhances the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Eyes have a complete loss of pigment.

Co-expression of Hsc70CbHMS01080 and DnaJ-1Scer\UAS.cKb does not suppress the external eye degeneration phenotype seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Complete neuronal degeneration is observed in these flies.

Expression of Hsap\DNAJB1Scer\UAS.cKa partially suppresses the loss of eye pigmentation seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsap\DNAJB4Scer\UAS.cKa does not suppress the loss of eye pigmentation seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsap\HSPH1Scer\UAS.cKa does not suppress the loss of eye pigmentation and external degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsap\HSPA4LScer\UAS.cKa does not suppress the loss of eye pigmentation and external degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsap\HSPA4LScer\UAS.cKa further suppresses the loss of eye pigmentation and external degeneration seen when Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 and Hsap\DNAJB1Scer\UAS.cKa are expressed under the control of Scer\GAL4GMR.PU.

Co-expression of either Mdr49HMS00400, Mdr50HMS01448 or Mdr65HMS01449 aggravates the eye degeneration phenotype seen in flies expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU, such that the proportion of flies showing a mild phenotype is decreased and the proportion showing a severe phenotype is increased. The number of inclusion bodies and disrupted rhabdomeres in the eye discs is increased and the disruption of the axonal projections of photoreceptor axons is more severe.

XNPC1/+ improves the hatching rate of embryos expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU and significantly reduces the fraction of deformed embryos.

The eye degeneration caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by co-expression of XNPEP635.

Co-expression of either DnaJ-1Scer\UAS.cKa or RpidsRNA.Sym.Scer\UAS suppresses the rough eye and loss of eye pigmentation phenotypes seen in adults expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

Co-expression of RpiScer\UAS.cWa does not exacerbate the rough eye phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 significantly enhances the pathogenic eye defects seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the eye (both lines under the control of Scer\GAL4GMR.PF), with approximately 92% of ommatidia displaying aberrant structures.

Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU significantly suppresses the eye degeneration phenotype resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

Co-expression of two copies of Hsp60DdsRNA.Sym.Scer\UAS with Scer\GAL4GMR.PU-driven Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 partially restores phototaxis.

Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4elav.PU partially suppresses the lethality resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression. The lethal phase is shifted from early to late pupa, although no flies emerge.

Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

The enhancement of the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU in a th5/+ background is not suppressed when Hsp60DdsRNA.Sym.Scer\UAS is co-expressed.

The pharate stage lethality due to co-expression of thdsRNA.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU is not suppressed by simultaneous expression of Hsp60DdsRNA.Sym.Scer\UAS.

The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye phenotype resulting from the expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PU is less pronounced in the presence of Uba2C175S.Scer\UAS.

The eye degeneration caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is enhanced by co-expression of Pros261.Scer\UAS.

Heterozygosity for th5 enhances the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU.

Co-expression of thdsRNA.Scer\UAS via Scer\GAL4GMR.PU in Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1-expressing flies results in death at pharate stage.

Simultaneous expression of Uba2C175S.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU enhances the eye degeneration phenotype.

Df(3R)Hrb87F dominantly enhances the eye phenotype caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, leading to formation of extensive black lesions on the eye surface. Furthermore, 24% of these flies die as differentiated pupae.

Inclusion of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and substantially reduces the mortality seen in flies expressing Scer\GAL4GMR.PF-driven Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in a Df(3R)Hrb87F/+ background.

Expression of HsrωEP3037 or HsrωEP93D in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Df(3R)Hrb87F/+ background significantly enhances the appearence of black lesions on the eye surface. Expression of HsrωEP3037 in this background also increases pupal mortality, whereas expression of HsrωEP93D has no effect on mortality.

Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death, whereas co-expression of HsrωEP3037 or HsrωEP93D results in a greater reduction in eye size, near complete loss of ommatidial arrays and bristles and the appearence of black lesions on the eye surface. Co-expression of HsrωEP3037 also enhances pupal lethality, whereas co-expression of HsrωEP93D does not enhance pupal death.

Expression of nejdsRNA.Scer\UAS.cKa in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner.

The eye damage caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is suppressed by co-expression of one copy of P{Sym-UAS-Hsrω}, but enhanced by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eyes.

The increase in inclusion bodies in larval eye discs caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is reduced by co-expression of one copy of P{Sym-UAS-Hsrω}, but further increased by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. However, co-expression of P{Sym-UAS-Hsrω} fails to reduce the number of inclusion bodies in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS background.

A Dp(3;3)st+g18 background (where Lmpt is duplicated) fails to suppress the degenerative eye phenotype found upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

Co-expression of two copies of P{Sym-UAS-Hsrω} nearly completely suppresses the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype in 54% of flies; photoreceptor neurons show improved morphology with the majority of ommatidia showing the normal seven rhabdomeres. Co-expression of one copy of P{Sym-UAS-Hsrω} also mitigates the damage caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, but to lesser extent.

Co-expression of either HsrωEP3037 or HsrωEP93D significantly enhances the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype, leading to extensive black lesions on the eye surface.

Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of HsrωEP3037 or HsrωEP93D expression on the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1.

Co-expression of one or two copies of P{Sym-UAS-Hsrω} restores normal phototactic behaviour to Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF flies.

Co-expression of a single copy of P{Sym-UAS-Hsrω} in the Scer\GAL4elav-C155, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background increases the proportion of flies surviving to adulthood from 0% to 98%.

The neurodegeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by Hsrω05241/+.

The presence of hoipGS7164 significantly enhances the neurodegeneration and rough eye phenotype seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

The presence of nop5Scer\UAS.cMa significantly enhances the neurodegeneration and rough eye phenotype seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

The presence of Nop56Scer\UAS.cMa significantly enhances the neurodegeneration and rough eye phenotype seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

Co-expression of Hsap\STUB1Scer\UAS.cAa does not suppress the eye phenotypes caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

The severity of the neurodegeneration phenotypes caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF are enhanced by Hsrω05241. In the adult eye, in a Hsrω05241/+ background, the eyes are significantly reduced in size and in some cases necrotic lesions are also seen. In the developing eye disc, in a Hsrω05241 homozygous background, a more severe disorganisation of the ommatidial units and their projections is seen; individual ommatidial cellular integrity is lost and axonal connections to the optic stalk appear highly irregular.

Eyes of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1/Scer\GAL4GMR.PF ; Df(3R)Hrb87F/+ flies exhibit dark necrotic patches covering almost all of the entire eye. This phenotype is not seen in the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1/Scer\GAL4GMR.PF mutant, indicating that Df(3R)Hrb87F/+ acts as a dominant enhancer of poly-Q toxicity.

The neurodegeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by l(3)pl10R/+; black necrotic patches cover almost the entire eye and eyes are reduced in size.

The neurodegeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by co-expression of either HsrωEP93D or HsrωEP3037 under the control of Scer\GAL4GMR.PF; extensive black necrotic lesions are seen in the eye.

The eye phenotype caused by expressing Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF, is not suppressed by coexpression of Hsap\BoatScer\UAS.T:Zzzz\FLAG.

When Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is driven in neurons either by Scer\GAL4Appl.G1a or Scer\GAL4179Y, and combined with Khc9, mutant larval nerves contain axonal blockages. Mutant nerves also contain enlarged axons, some almost four or five times the diameter of those seen in wild-type. Sometimes "holes" lacking organelles are seen within the nerve.

The addition of nejEP1149 rescues the eye phenotype seen in Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF mutants. The addition of nejEP1179 rescues the eye and the phototaxis phenotypes seen in Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF mutants. The addition of nejP enhances the eye phenotype seen in Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF mutants.

Expression of MlfScer\UAS.cKa under the control of Scer\GAL4GMR.PF suppresses the structural and pigmentation defects observed in Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 mutants.

The presence of MlfEU2490 dramatically reduces the external eye and pigmecntation defects caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

DnaJ-1EU3500 suppresses of the eye degeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF; the eye preserves its globular structure, pigmentation and uniform bristle arrangement and the structure of the retina is vastly improved. Tpr2EU3220 suppresses of the eye degeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF; external structure and pigmentation is improved. There is some improvement of internal retinal structure (some degeneration is seen). Co-expression of DnaJ-1Scer\UAS.cKa or Tpr2Scer\UAS.cKa suppresses of the eye degeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
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Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1
Zzzz\CAG127Q.UAS.Tag:HA
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