Inhibition (using
shits1.IVS.p10.20xUAS at 32[o]C) of
Scer\GAL4Ddc.PL cells in adults significantly reduces 3-min associative olfactory water-reward memory, compared to controls; inhibition during acquisition alone also significantly reduces 3-hour and 24-hour memory.
Thermogenetic activation (using
TrpA1UAS.(B).cKa at 31[o]C) of
Scer\GAL4Ddc.PL cells in thirsty or water-sated adults, in combination with a paired odor, results in a significant increase in 3-min, 3-hour and 24-hour associative attractive olfactory memory, compared to controls.
Inhibition (using
Ctet\tetXTNT.UAS) of a subset of
Scer\GAL4Ddc.PL cells (expression restricted using
Scer\GAL80ple.PK) does not affect latency of escape from heat or associative aversive (heat) place memory, compared to controls; the increases in escape latency and aversive memory due to unexpected high (41[o]C) temperature pre-exposure are significantly lower than those of controls.
Inhibition (using
shi1.UAS at 33[o]C) of
Scer\GAL4Ddc.PL cells in adults significantly reduces appetitive, but not aversive, 1-min visual memory, compared to controls; inhibition during training alone, but not testing, significantly reduces appetitive, but not aversive, 30-min visual memory, compared to controls; inhibition does not significantly affect sugar preference, compared to controls.
Thermogenetic activation (using
TrpA1UAS.(B).cKa at 30[o]C) of
Scer\GAL4Ddc.PL cells in starved, but not fed, adults, in combination with a paired odor, results in a significant increase in 2-min associative attractive olfactory memory, compared to controls; this memory increase is still seen when activation is restricted to a smaller number of
Scer\GAL4Ddc.PL cells using
Scer\GAL80ple.PK, but not
Scer\GAL80R58E02; a significant increase in 12-hour memory also occurs when flies are starved or fed prior to training, but not if they are fed immediately before testing.
Inhibition (using
shi1.UAS at 32[o]C) of
Scer\GAL4Ddc.PL cells in adults fed immediately before training does not significantly affect 3-hour associative appetitive olfactory memory, compared to controls; inhibition before and during training, but not after training and during testing, significantly reduces 30-min associative appetitive olfactory memory, compared to controls; inhibition also significantly reduces short-term associative appetitive olfactory memory, compared to controls, but not when restricted to a smaller subset of
Scer\GAL4Ddc.PL cells using
Scer\GAL80R58E02; inhibition has no effect on naive sugar preference, compared to controls.
Animals expressing two copies of
Scer\GAL4Ddc.PL exhibit a greatly reduced lifespan, compared to wild-type. their locomotion remains normal.
