FlyBase Allele Report: Dmel\Dronc[ΔN.UAS]

General Information

Symbol

Dmel\Dronc^ΔN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0104749

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-ΔNdronc}

Also Known As

UAS-ΔN-dronc

Key Links

Transgenic product class

inframe_deletion

(Meier, 2000.4.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Meier, 2000.4.5)

Mutagen

in vitro construct

(Meier, 2000.4.5)

Progenitor genotype

Carried in construct

P{UAS-ΔNdronc}

Cytology

Description

A truncated coding sequence of Dronc from nucleotides 706 to 1721, missing its prodomain is expressed under the control of UASt sequences.

(Meier, 2000.4.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dronc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head | temperature conditional, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

eye, with Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008, Meier et al., 2000)

eye | temperature conditional, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

eye disc, with Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008, Meier et al., 2000)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PU

(Xu et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Meier et al., 2000)

pigment cell, with Scer\GAL4^GMR.PF

(Meier et al., 2000)

Detailed Description

Statement

Reference

Expression of Nc^ΔN.Scer\UAS in larval wing disc cells, under the control of Scer\GAL4^ptc-559.1, does not affect cell migration.

(Rudrapatna et al., 2013)

Overexpression of Nc^ΔN.Scer\UAS in the whole brain, under the control of Scer\GAL4^Dll-md23 does not affect neuroblast formation.

(Ouyang et al., 2011)

Expression of Nc^ΔN.Scer\UAS under the control Scer\GAL4^c42 results in first instar larval lethality.

(Shukla and Tapadia, 2011)

Scer\GAL4^GMR.PF-mediated expression of Nc^{DeltaN.Scer\UAS} results in severely malformed and depigmented eyes.

At 25[o]C, Scer\GAL4^GMR.PF,Nc^{DeltaN.Scer\UAS} flies fail to eclose out of the pupal case. At 18[o]C, a few flies emerge with completely ablated eyes and reduced head size.

(Mallik and Lakhotia, 2009)

Flies expressing Nc^ΔN.Scer\UAS under the control of Scer\GAL4^GMR.PF show degeneration of the eye. Large numbers of apoptotic cells are present in the eye discs of these animals.

(Arya and Lakhotia, 2008)

Expression of Nc^ΔN.Scer\UAS under the control of Scer\GAL4^GMR.PU induces apoptosis in the third larval instar eye disc.

(Xu et al., 2006)

When expression of Nc^ΔN.Scer\UAS is driven by Scer\GAL4^GMR.PF flies exhibit roughened eyes that are reduced in size. Their surface morphology is distorted, disrupted and rough. The eyes are white. Dramatic and supernumerary apoptosis is seen posterior to the morphogenetic furrow in the eye disc.

(Meier et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has abnormal eye color phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has lethal | pupal stage | temperature conditional phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has lethal | pharate adult stage phenotype, suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008)

NOT suppressed by

Statement

Reference

Dronc^ΔN.UAS, Scer\GAL4^GMR.PU has increased cell death | third instar larval stage phenotype, non-suppressible by Drice¹⁷/Drice¹⁷

(Xu et al., 2006)

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has adult head | temperature conditional phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has eye disc phenotype, suppressible by Hsp60D^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Arya and Lakhotia, 2008)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by BacA\p35^GMR.PH

(Meier et al., 2000)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^GMR.PH

(Meier et al., 2000)

NOT suppressed by

Statement

Reference

Dronc^ΔN.UAS, Scer\GAL4^GMR.PU has eye disc | third instar larval stage phenotype, non-suppressible by Drice¹⁷/Drice¹⁷

(Xu et al., 2006)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has pigment cell phenotype, non-suppressible by BacA\p35^GMR.PH

(Meier et al., 2000)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by BacA\p35^GMR.PH

(Meier et al., 2000)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has pigment cell phenotype, non-suppressible by Diap1^GMR.PH

(Meier et al., 2000)

Dronc^ΔN.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Diap1^GMR.PH

(Meier et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS is an enhancer of neuron | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS is a suppressor of type II neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nc^ΔN.Scer\UAS attenuates numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation. No change in apoptosis is reported in this background, although the number of post-mitotic neurons is increased.

(Ouyang et al., 2011)

Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in Scer\GAL4^GMR.PF, Nc^{DeltaN.Scer\UAS} flies.

Co-expression of P{Sym-UAS-Hsrω} rescues the pupal lethality and small head phenotypes seen in flies expressing Nc^{DeltaN.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Mallik and Lakhotia, 2009)

Co-expression of Hsp60D^{dsRNA.Sym.Scer\UAS} suppresses the eye phenotypes caused by expression of Nc^ΔN.Scer\UAS under the control of Scer\GAL4^GMR.PF, and all these animals eclose with normal head size.

(Arya and Lakhotia, 2008)

Homozygosity for Ice¹⁷ does not suppress the apoptosis induced in the third larval instar eye disc by expression of Nc^ΔN.Scer\UAS under the control of Scer\GAL4^GMR.PU.

(Xu et al., 2006)

The addition of th^GMR.PH to Nc^ΔN.Scer\UAS, Scer\GAL4^GMR.PF fails to rescue the pigment cell and ommatidial phenotypes seen in these flies. However the eye size is slightly increased compared to Nc^ΔN.Scer\UAS and Scer\GAL4^GMR.PF alone. The addition of BacA\p35^GMR.PH to Nc^pro.Scer\UAS,Scer\GAL4^GMR.PF weakly suppresses the phenotypes seen in those flies, leading to a slightly increased eye size.

(Meier et al., 2000)

Xenogenetic Interactions

Statement

Reference

Co-expression of Nc^ΔN.Scer\UAS with BacA\p35^Scer\UAS.cHa in larval wing disc cells, under the control of Scer\GAL4^ptc-559.1, leads to an aggressive cell invasion phenotype with extensive lamellipodia-like cellular processes.

(Rudrapatna et al., 2013)

The addition of BacA\p35^GMR.PH to Nc^ΔN.Scer\UAS,Scer\GAL4^GMR.PF weakly suppresses the phenotypes seen in those flies, leading to a slightly increased eye size.

(Meier et al., 2000)

Complementation and Rescue Data

Rescues

Dronc^ΔN.UAS rescues Dronc^I29/Dronc^L32

(Napoletano et al., 2017)

Comments

The expression of Dronc^ΔN.Scer\UAS under the control of Scer\GAL4^nos.PU rescues both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of testes observed in Dronc^I29/Dronc^L32 transheterozygotes.

(Napoletano et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dronc^ΔN.Scer\UAS

Dronc^ΔN.UAS

Nc^ΔN.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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