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General Information
Symbol
Hsap\SNCAA53T.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0104767
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-A53T, UAS-A53T α-synuclein
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the mutated A53T form of Hsap\SNCA (which is linked to a familial Parkinson's disease in humans).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

The locomotor defects seen in larvae expressing Hsap\SNCA[A53T.Scer\UAS] in dopaminergic neurons are exacerbated by rotenone.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Hsap\SNCAA53T.UAS under the control of Scer\GAL4elav.PLu leads to a progressive decrease in adult climbing ability.

Expressing Hsap\SNCAA53T.UAS under the control of Scer\GAL4ple.PF induces a progressive decrease in adult climbing capacity and results in 21-days old adults raised at 29[o]C exhibiting significantly fewer PPL1 neurons than age-matched controls; there is no obvious effect on lifespan.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4GMR.PU induces a rough eye phenotype in the adult eye and expression under the Scer\GAL4Ddc.HL9 driver results in an age-progressive decrease in climbing abilities of adult flies.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav.PLu exhibit a progressive reduction of locomotion in climbing assays.

Adult flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF fed with 1% glucose show a significantly lower survival rate and loss of dopaminergic neurons in the PPM1/2 cluster (other dopaminergic neuronal clusters - VUM, PAL, PPM3, PPL1 and PPL2 - are unaffected) but their locomotion (climbing ability) at 0-5 days post-eclosion is not impaired relative to wild-type.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav-C155 results in a significant reduction in lifespan as compared to controls. These flies exhibit a significantly lower sedation time in response to acute ethanol exposure, and also show a significant longer recovery time, as compared to controls.

Flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav-C155 show defects in climbing ability. This phenotype is partially suppressed when the flies are fed the natural antioxidant decalepis hamiltonii (Dh). Climbing ability and early death are also seen when the flies are fed paraquat, and these phenotypes are both partly suppressed by Dh.

Locomotor rhythm and level of physical activity are disrupted in flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav-C155. This is partially suppressed when the flies are fed the natural antioxidant decalepis hamiltonii (Dh). Climbing ability and early death are also seen when the flies are fed paraquat, and these phenotypes are both partly suppressed by Dh.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF does not result in a loss of dopaminergic neurons in 55-day old flies.

Third instar larvae expressing Hsap\SNCAA53T.Scer\UAS in dopaminergic neurons under the control of Scer\GAL4ple.PF have significantly slower locomotion compared to wild type. This phenotype is exacerbated upon exposure to rotenone.

The brains of third instar larvae expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF have a decreased number of dopaminergic neurons in each of the three clusters (dorsomedial, dorsolateral 1 and dorsolateral 2). The phenotype is age-dependent; the number of DA neurons in first instar larvae is similar to wild type. A similar phenotype is seen when larvae expressing Hsap\SNCAA53T.Scer\UAS are exposed to rotenone, but brain and body size are similar to wild type.

Third instar larvae expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF do not show any difference in olfaction compared to wild type.

Flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav-C155 show reduced climbing ability compared to age-matched controls from 9 days of age onwards. The severity of the defect increases with age. The severity of the defect is significantly reduced if the flies are raised on medium containing 0.75 mg/mL cinnamon extract precipitation.

Flies expressing Hsap\SNCAA53T.Scer\UAS in dopaminergic neurons under the control of Scer\GAL4ple.PF exhibit age-dependent neurodegeneration. No neuron loss is seen at days 15 or 25, but increased neurodegeneration compared to controls is seen at 40 days. No loss of dopaminergic neurons is seen in age-matched wild type flies.

No neurodegeneration is observed when Hsap\SNCAA53T.Scer\UAS is expressed either in the photoreceptors under the control of Scer\GAL4ninaE.PD, or in the small ventrolateral neurons (sLNvs) under the control of Scer\GAL4P2.4.Pdf.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF enhances the hypoxia-induced dopaminergic neuron degeneration seen in control flies.

Expression of Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF results in loss of dopaminergic neurons in aged brains.

20 day old flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4Ddc.PL have a 50% reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain.

20 day old flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4Ddc.PL show marked loss of neurons in the dorsomedial dopaminergic clusters. Neuron loss is more variable in the dorsolateral-1 dopaminergic clusters, ranging from no loss of neurons to as much as 50% of neurons being lost.

Flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav.PLu show a marked, age-dependent loss of dorsomedial dopaminergic neurons. Hsap\SNCA inclusions in the brain (detected by immunostaining) are seen in aged flies (the inclusions are first seen at 20-30 days of age). Most neuronal cytoplasmic inclusions are single, round and regular, although a few are more irregular. Neuritic Hsap\SNCA inclusions are also present in the brain and consist of both thread- and grain-like inclusions. Locomotor behaviour is grossly preserved in young flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav.PLu. These flies initially climb as well as wild-type flies, but over time they show a premature loss of climbing ability compared to control flies. Flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4GMR.PF show retinal degeneration that is detectable by 10 days, and marked at 30 days after eclosion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The rough eye phenotype characteristic for adult flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4GMR.PU is restored by co-expression of Usp8HMS01898 RNAi but not by expression of any of the following: CG2224KK101867, Usp14GLC01823 or Usp47HMC02352.

The age-progressive decrease in climbing abilities of adult flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4Ddc.HL9 as well as the loss of neurons in the PPM1/2 cluster is partially or fully (respectively) rescued by co-expression of Usp8HMS01898 RNAi. The climbing assay performance is not improved by co-expression of either CG3781KK108083 or CG2224KK101867.

Expression of Rab7Scer\UAS.T:Avic\GFP does not alter lifespan or lead to a significant difference in the the locomotion activity of flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav.PLu at 5 days old, however, at 15 and 25 days old results in significantly improved locomotion, delaying the onset of locomotion defects seen in flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4elav.PLu.

The loss of dopaminergic neurons that is seen in the brains of flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4ple.PF is suppressed by co-expression of Mmus\Rab1Scer\UAS.cCa.

The reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 20 day old flies expressing Hsap\SNCAA53T.Scer\UAS under the control of Scer\GAL4Ddc.PL is suppressed by coexpression of Hsap\PARK2Scer\UAS.cYa.

Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Hsap\SNCAA53T.Scer\UAS
Hsap\SNCAA53T.UAS
αSynA53T
Name Synonyms
Secondary FlyBase IDs
    References (20)