FB2025_01 , released February 20, 2025
Allele: Dmel\His2AvGFP(S65T)
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General Information
Symbol
Dmel\His2AvGFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0104781
Feature type
allele
Associated gene
Dmel\His2Av
Associated Insertion(s)
Carried in Construct
P{His2Av.GFP(S65T)}
Also Known As
histone-GFP, His2AvDGFP, H2Av-GFP, GFP-histone, His2AvD-GFP, His-GFP, H2AvD-GFP, H2A-GFP, His2Av-GFP, Hist-GFP
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Parry and O'Farrell, 2001, Clarkson and Saint, 1999)
Progenitor genotype
Carried in construct
P{His2Av.GFP(S65T)}
Cytology
Description

A 4.1kb BglII genomic fragment containing the His2Av gene tagged at the 3' end of the open reading frame (ORF) with GFP(S65T). The last two codons of the His2Av ORF have been removed and replaced with an additional 5 codons before the 5' end of the GFP(S65T) ORF.

(Clarkson and Saint, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
His2Av
Encoded product / tool
His2Av
Tagged with
GFP(S65T)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      His2AvGFP(S65T) rescues His2Av810

      (Clarkson and Saint, 1999)
      Fails to rescue

      His2AvGFP(S65T) fails to rescue Df(3R)nm136

      (Shahab et al., 2015)
      Comments

      His2AvT:Avic\GFP-S65T does not rescue the larval lethality of Df(3R)nm136 animals.

      (Shahab et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid and transfected into S2 cells to study the subcellular localization of the expressed protein.

      (Cermelli et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      His2AvGFP(S65T)
      His2AvT:Avic\GFP-S65T
      (Bischoff and Cseresnyés, 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (93)