FlyBase Allele Report: Dmel\DnaJ-1[UAS.cKa]

General Information

Symbol

Dmel\DnaJ-1^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kazemi-Esfarjani

FlyBase ID

FBal0104861

Feature type

allele

Associated gene

Dmel\DnaJ-1

Associated Insertion(s)

Carried in Construct

P{UAS-DnaJ-1.K}

Also Known As

UAS-dhdJ1, UAS-DnaJ-1

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Kazemi-Esfarjani and Benzer, 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-DnaJ-1.K}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of DnaJ-1 coding sequences.

(Kazemi-Esfarjani and Benzer, 2000)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

DnaJ-1

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates spinocerebellar ataxia type 6

modeled by Hsap\CACNA1A^{UAS.αACT.Q70}

(Tsou et al., 2015)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Ghosh and Feany, 2004)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004, Kazemi-Esfarjani and Benzer, 2000)

modeled by Hsap\HTT^Q93.ex1.UAS

(Liévens et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^lz-gal4 does not significantly affect the number or size of third instar larval circulating crystal cells.

(Miller et al., 2017)

Expression of DnaJ-1^Scer\UAS.cKa in larval body wall sensory neurons under the control of Scer\GAL4^RluA1 does not affect larval locomotion.

(Nishimura et al., 2010)

Flies expressing DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW show normal wing positions.

(Chartier et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of neuroanatomy defective | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q76.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of neuroanatomy defective | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of neuroanatomy defective | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^sqh.PW is a suppressor of lethal - all die during pharate adult stage phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^sqh.PW

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of lethal phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Prosβ2^1.UAS, Prosβ6^1.B.UAS, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012, Kazemi-Esfarjani and Benzer, 2000)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye color defective phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

DnaJ-1^UAS.cKa, Scer\GAL4^RluA1 is a suppressor of locomotor rhythm defective phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^RluA1

(Nishimura et al., 2010)

DnaJ-1^UAS.cKa, Scer\GAL4^RluA1 is a suppressor of uncoordinated phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^RluA1

(Nishimura et al., 2010)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of melanotic necrosis phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

DnaJ-1^UAS.cKa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

DnaJ-1^UAS.cKa, Scer\GAL4^Mhc.PW is a suppressor | partially of visible phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^Appl.G1a is a suppressor of short lived | female phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Fayazi et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PY is a suppressor | drug conditional | partially of visible phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PY

(Takeyama et al., 2002)

Other

Statement

Reference

DnaJ-1^UAS.cKa, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsf^JF02415, Scer\GAL4^GMR.long has visible phenotype

(Tsou et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of class IV dendritic arborizing neuron | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q76.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of class IV dendritic arborizing neuron | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of retina | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012, Kazemi-Esfarjani and Benzer, 2000)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of retina | temperature conditional phenotype of Gug^75QN.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of eye phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of pigment cell phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

DnaJ-1^UAS.cKa, Scer\GAL4^Mhc.PW is a suppressor | partially of wing phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^30Y is a suppressor of adult brain phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^30Y

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PY is a suppressor | drug conditional | partially of eye phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PY

(Takeyama et al., 2002)

Other

Statement

Reference

DnaJ-1^UAS.cKa, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsf^JF02415, Scer\GAL4^GMR.long has eye phenotype

(Tsou et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is suppressed by co-expression of DnaJ-1^Scer\UAS.cKa.

(Nisoli et al., 2010)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses of the eye degeneration phenotype caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

(Kazemi-Esfarjani and Benzer, 2000)

Xenogenetic Interactions

Statement

Reference

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses lethality (adults survive 2-3 weeks) in flies with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^sqh.PW. Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the progressive retinal degeneration seen in fly eyes with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^GMR.PU.

(Tsou et al., 2015)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses eye degeneration (external and internal) in flies (both 1 day and 2 weeks old) with expression of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long. Co-expression of Rad23^KK107826, Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6}, Hsap\ATXN3^{C14A.Scer\UAS.T:Zzzz\His6} or Hsap\ATXN3^{W87A.Scer\UAS.T:Zzzz\His6}, Rpn8^KK101319, Rpn11^GD8888, simultaneous co-expression of Prosβ6^1.B.Scer\UAS and Prosβ2^1.Scer\UAS, or presence of Prosβ6¹ does not suppress the protective role of expression of DnaJ-1^Scer\UAS.cKa in suppressing eye degeneration in flies with Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long. Co-expression of Hsf^JF02415 suppresses the protective role of expression of DnaJ-1^Scer\UAS.cKa in suppressing eye degeneration in flies with Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long. Simultaneous co-expression of Prosβ6^1.B.Scer\UAS and Prosβ2^1.Scer\UAS leads to lethality in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies; extra co-expression of DnaJ-1^Scer\UAS.cKa suppresses this lethality.

(Tsou et al., 2015)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the rough eye and loss of eye pigmentation phenotypes seen in adults expressing Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

(Wang et al., 2012)

Co-expression of DnaJ-1^Scer\UAS.cKa with Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1} in larval body wall sensory neurons (under the control of Scer\GAL4^RluA1) restores the speed of locomotion to wild-type levels.

(Nishimura et al., 2010)

Expression of DnaJ-1^Scer\UAS.cKa in the developing eye under the control of Scer\GAL4^GMR.PF suppresses the eye phenotype found in flies mutant for both Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP} and Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}.

(Doumanis et al., 2009)

Expression of DnaJ-1^Scer\UAS.cKa partially suppresses the reduction in life expectancy seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^Eaat1.PR.

(Liévens et al., 2008)

Coexpression of DnaJ-1^Scer\UAS.cKa transgene results in a weak suppression of the wing posture phenotype of Scer\GAL4^Mhc.PW>Hsap\PABPN1^{17ala.Scer\UAS} flies.

(Chartier et al., 2006)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the short-lived phenotype of females expressing Zzzz\CAG^{63Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a.

(Fayazi et al., 2006)

The rough eye phenotype seen Hsap\ATX1^82.Scer\UAS, Scer\GAL4^GMR.PF animals is suppressed by DnaJ-1^Scer\UAS.cKa.

(Tsai et al., 2004)

DnaJ-1^Scer\UAS.cKa partially suppresses the rough eye phenotype of Hsap\AR^Q52.Scer\UAS; Scer\GAL4^GMR.PY animals treated with the androgen receptor ligand dihydroxytestosterone (DHT).

(Takeyama et al., 2002)

Complementation and Rescue Data

Rescues

DnaJ-1^UAS.cKa/Scer\GAL4^lz-gal4 rescues DnaJ-1^C/DnaJ-1^A

(Miller et al., 2017)

Comments

The expression of DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^lz-gal4 rescues the increased number and size of circulating crystal cells present in DnaJ-1^A/DnaJ-1^C transheterozygous third instar larvae.

(Miller et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30553

w^*; P{UAS-DnaJ-1.K}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DnaJ-1^Scer\UAS.cKa

DnaJ-1^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kazemi-Esfarjani

(Kazemi-Esfarjani and Benzer, 2000)

Secondary FlyBase IDs

References (17)

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