FlyBase Allele Report: Dmel\DnaJ-1[UAS.cKa]

General Information

Symbol

Dmel\DnaJ-1^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kazemi-Esfarjani

FlyBase ID

FBal0104861

Feature type

allele

Associated gene

Dmel\DnaJ-1

Associated Insertion(s)

Carried in Construct

P{UAS-DnaJ-1.K}

Also Known As

UAS-dhdJ1, UAS-DnaJ-1

Key Links

Transgenic product class

wild_type

(Kazemi-Esfarjani and Benzer, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kazemi-Esfarjani and Benzer, 2000)

Mutagen

in vitro construct

(Kazemi-Esfarjani and Benzer, 2000)

Progenitor genotype

Carried in construct

P{UAS-DnaJ-1.K}

Cytology

Description

UAS regulatory sequences drive expression of DnaJ-1 coding sequences.

(Kazemi-Esfarjani and Benzer, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

DnaJ-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates spinocerebellar ataxia type 6

modeled by Hsap\CACNA1A^{UAS.αACT.Q70}

(Tsou et al., 2015)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

modeled by Hsap\ATXN3^{80Q.UbS1mut.UAS}

(Prifti et al., 2022)

modeled by Hsap\ATXN3^{UbS2-mild.fl.SCA3.UAS}

(Sutton et al., 2017)

modeled by Hsap\ATXN3^{tr.Q76.UAS.Tag:HA,Tag:MYC}

(Kwon et al., 2018)

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Yeh et al., 2018)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Ghosh and Feany, 2004)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004, Kazemi-Esfarjani and Benzer, 2000)

modeled by Hsap\HTT^Q93.ex1.UAS

(Liévens et al., 2008)

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Prakash et al., 2022)

ameliorates Creutzfeldt-Jakob disease

modeled by Oari\PrP^VRQ.UAS

(Thackray et al., 2022)

modeled by Hsap\PRNP^M129.UAS.cTa

(Thackray et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^lz-gal4 does not significantly affect the number or size of third instar larval circulating crystal cells.

(Miller et al., 2017)

Expression of DnaJ-1^Scer\UAS.cKa in larval body wall sensory neurons under the control of Scer\GAL4^RluA1 does not affect larval locomotion.

(Nishimura et al., 2010)

Flies expressing DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW show normal wing positions.

(Chartier et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKa, Scer\GAL4^{elav.Switch.PO} is a suppressor | partially of short lived phenotype of Hsap\ATXN3^{80Q.1.UAS.Tag:HA}, Scer\GAL4^{elav.Switch.PO}

(Prifti et al., 2022)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of visible | adult stage phenotype of Hsap\ATXN3^{80Q.UbS1mut.UAS}, Scer\GAL4^GMR.long

(Prifti et al., 2022)

DnaJ-1^UAS.cKa, Scer\GAL4^{elav.Switch.PO} is a suppressor | partially of short lived phenotype of Hsap\ATXN3^{80Q.UbS1mut.UAS}, Scer\GAL4^{elav.Switch.PO}

(Prifti et al., 2022)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q76.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^sqh.PW is a suppressor of lethal - all die during pharate adult stage phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^sqh.PW

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of lethal phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Prosβ2^1.UAS, Prosβ6^1.B.UAS, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012, Kazemi-Esfarjani and Benzer, 2000)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of melanotic necrosis phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

DnaJ-1^UAS.cKa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

DnaJ-1^UAS.cKa, Scer\GAL4^Mhc.PW is a suppressor | partially of visible phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^Appl.G1a is a suppressor of short lived | female phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Fayazi et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PY is a suppressor | drug conditional | partially of visible phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PY

(Takeyama et al., 2002)

Other

Statement

Reference

DnaJ-1^UAS.cKa, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsf^JF02415, Scer\GAL4^GMR.long has visible phenotype

(Tsou et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{80Q.UbS1mut.UAS}, Scer\GAL4^GMR.long

(Prifti et al., 2022)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of larval multidendritic class IV neuron | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q76.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^ppk.PU is a suppressor | partially of larval multidendritic class IV neuron | third instar larval stage phenotype of Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}, Scer\GAL4^ppk.PU

(Kwon et al., 2018)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of retina | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012, Kazemi-Esfarjani and Benzer, 2000)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor of retina | temperature conditional phenotype of Gug^75QN.UAS, Scer\GAL4^GMR.PU

(Nisoli et al., 2010)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of eye phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

Scer\GAL4^GMR.PF, DnaJ-1^UAS.cKa is a suppressor of pigment cell phenotype of Hsap\HTT^{152Q.Ex1.UAS.EGFP}, Hsap\HTT^{48Q.Ex1.UAS.EGFP.Tag:NLS(Unk)}, Scer\GAL4^GMR.PF

(Doumanis et al., 2009)

DnaJ-1^UAS.cKa, Scer\GAL4^Mhc.PW is a suppressor | partially of wing phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

DnaJ-1^UAS.cKa, Scer\GAL4^30Y is a suppressor of adult brain phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^30Y

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^ChAT.7.4 is a suppressor of adult brain phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Tsai et al., 2004)

DnaJ-1^UAS.cKa, Scer\GAL4^GMR.PY is a suppressor | drug conditional | partially of eye phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PY

(Takeyama et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Other

Statement

Reference

DnaJ-1^UAS.cKa, Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsf^JF02415, Scer\GAL4^GMR.long has eye phenotype

(Tsou et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Gug^{75QN.Scer\UAS} in the adult eye under the control of Scer\GAL4^GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is suppressed by co-expression of DnaJ-1^Scer\UAS.cKa.

(Nisoli et al., 2010)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses of the eye degeneration phenotype caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

(Kazemi-Esfarjani and Benzer, 2000)

Xenogenetic Interactions

Statement

Reference

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses lethality (adults survive 2-3 weeks) in flies with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^sqh.PW. Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the progressive retinal degeneration seen in fly eyes with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^GMR.PU.

(Tsou et al., 2015)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses eye degeneration (external and internal) in flies (both 1 day and 2 weeks old) with expression of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long.

Co-expression of Rad23^KK107826, Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6}, Hsap\ATXN3^{C14A.Scer\UAS.T:Zzzz\His6} or Hsap\ATXN3^{W87A.Scer\UAS.T:Zzzz\His6}, Rpn8^KK101319, Rpn11^GD8888, simultaneous co-expression of Prosβ6^1.B.Scer\UAS and Prosβ2^1.Scer\UAS, or presence of Prosβ6¹ does not suppress the protective role of expression of DnaJ-1^Scer\UAS.cKa in suppressing eye degeneration in flies with Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long.

Co-expression of Hsf^JF02415 suppresses the protective role of expression of DnaJ-1^Scer\UAS.cKa in suppressing eye degeneration in flies with Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.long.

Simultaneous co-expression of Prosβ6^1.B.Scer\UAS and Prosβ2^1.Scer\UAS leads to lethality in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies; extra co-expression of DnaJ-1^Scer\UAS.cKa suppresses this lethality.

(Tsou et al., 2015)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the rough eye and loss of eye pigmentation phenotypes seen in adults expressing Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

(Wang et al., 2012)

Co-expression of DnaJ-1^Scer\UAS.cKa with Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1} in larval body wall sensory neurons (under the control of Scer\GAL4^RluA1) restores the speed of locomotion to wild-type levels.

(Nishimura et al., 2010)

Expression of DnaJ-1^Scer\UAS.cKa in the developing eye under the control of Scer\GAL4^GMR.PF suppresses the eye phenotype found in flies mutant for both Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP} and Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}.

(Doumanis et al., 2009)

Expression of DnaJ-1^Scer\UAS.cKa partially suppresses the reduction in life expectancy seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^Eaat1.PR.

(Liévens et al., 2008)

Coexpression of DnaJ-1^Scer\UAS.cKa transgene results in a weak suppression of the wing posture phenotype of Scer\GAL4^Mhc.PW>Hsap\PABPN1^{17ala.Scer\UAS} flies.

(Chartier et al., 2006)

Co-expression of DnaJ-1^Scer\UAS.cKa suppresses the short-lived phenotype of females expressing Zzzz\CAG^{63Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a.

(Fayazi et al., 2006)

The rough eye phenotype seen Hsap\ATX1^82.Scer\UAS, Scer\GAL4^GMR.PF animals is suppressed by DnaJ-1^Scer\UAS.cKa.

(Tsai et al., 2004)

DnaJ-1^Scer\UAS.cKa partially suppresses the rough eye phenotype of Hsap\AR^Q52.Scer\UAS; Scer\GAL4^GMR.PY animals treated with the androgen receptor ligand dihydroxytestosterone (DHT).

(Takeyama et al., 2002)

Complementation and Rescue Data

Rescues

DnaJ-1^UAS.cKa/Scer\GAL4^lz-gal4 rescues DnaJ-1^C/DnaJ-1^A

(Miller et al., 2017)

Comments

The expression of DnaJ-1^Scer\UAS.cKa under the control of Scer\GAL4^lz-gal4 rescues the increased number and size of circulating crystal cells present in DnaJ-1^A/DnaJ-1^C transheterozygous third instar larvae.

(Miller et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30553

w^*; P{UAS-DnaJ-1.K}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DnaJ-1^Scer\UAS.cKa

DnaJ-1^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kazemi-Esfarjani

(Kazemi-Esfarjani and Benzer, 2000)

Secondary FlyBase IDs

References (22)

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