Allele Dmel\Antp^Scer\UAS.cBa

General Information
Symbol	Dmel\Antp^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Bello	FlyBase ID	FBal0104893
Feature type	allele	Associated gene	Dmel\Antp

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms arista pretarsus
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bello, 2000.3.15)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A 2.2kb NotI fragment which contains the Antp cDNA is driven by Scer\UAS sequences. (Bello, 2000.3.15)
Carried in construct	P{UAS-Antp.B} (Bello et al., 2003, Katsuyama et al., 2005, Alexandre and Vincent, 2003, Plaza et al., 2001, Bello, 2000.3.15, Kurata et al., 2000, Prince et al., 2008, Plaza et al., 2008, Percival-Smith et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^ey-OK107 (Plaza et al., 2008) visible, with Scer\GAL4^dpp.blk1 (Plaza et al., 2008, Percival-Smith et al., 2005) visible, with Scer\GAL4^ey-OK107 (Plaza et al., 2008)
Phenotype Manifest In
	antenna, with Scer\GAL4^dpp.blk1 (Plaza et al., 2001, Plaza et al., 2008) antenna, with Scer\GAL4^ey.PH (Prince et al., 2008) arista, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) eye, with Scer\GAL4^dpp.blk1 (Plaza et al., 2001, Plaza et al., 2008) eye, with Scer\GAL4^ey.PH (Plaza et al., 2001) eye, with Scer\GAL4^ey-OK107 (Plaza et al., 2001, Prince et al., 2008, Plaza et al., 2008) eye, with Scer\GAL4^GMR.PF (Plaza et al., 2001) leg \| ectopic, with Scer\GAL4^dpp.blk1 (Plaza et al., 2001, Plaza et al., 2008) leg \| ectopic, with Scer\GAL4^ey.PH (Prince et al., 2008) pretarsus \| ectopic, with Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) thoracic neuroblast \| larval stage, with Scer\GAL4^αTub84B.PL (Bello et al., 2003) wing \| ectopic, with Scer\GAL4^ey-OK107 (Prince et al., 2008)
Detailed Description
	Statement Reference Expression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[dpp.blk1] results in antenna-to-leg transformation and a reduction in the eye. Expression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107] results in approximately 95% pupal lethality. 75-100% of escapers show a reduction in the eye compared to wild type. (Plaza et al., 2008) Misexpression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey.PH] promotes antenna-to-leg transformation but does not induce wing development. Ectopic expression of Antp[Scer\UAS.cBa] in the eye disc using the eye-specific driver Scer\GAL4[OK107] results in 8% of flies exhibiting an eye-to-wing transformation, in addition to an eye reduction phenotype. (Prince et al., 2008) Expression of Antp^Scer\UAS.cBa under the control of Scer\GAL4^ey.PB induces ectopic wings with antero-posterior and dorsal ventral axes. (Katsuyama et al., 2005) Clones in the arista expressing Antp[Scer\UAS.cBa] under the control of Scer\GAL4[Act5C.PP] are wild type (they are not transformed to tarsus or leg identity). Expression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[dpp.blk1] results in transformation of the arista to tarsus. (Percival-Smith et al., 2005) Postembryonic neuroblast (pNB) clones in the larval thorax expressing Antp^Scer\UAS.cBa under the control of Scer\GAL4^αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases. (Bello et al., 2003) When Antp^Scer\UAS.cBa is driven by Scer\GAL4^ey.PH a reduction in eye size is seen. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^dpp.blk1 a reduction in eye size is seen as well as antenna to leg transformations. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^GMR.PF only a minor defect in eye size is seen. When Antp^Scer\UAS.cBa is driven by Scer\GAL4^OK107 an eyeless phenotype is seen. (Plaza et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^Scer\UAS.cHa, Scer\GAL4^ey-OK107 (Plaza et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^{ΔCT.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^{ΔHD.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by ey^{ΔPD.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008)
NOT suppressed by
Statement Reference Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has lethal phenotype, non-suppressible by ey^{ΔHD.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008)
Other
Statement Reference Antp^Scer\UAS.cBa, N^ICN.Scer\UAS, Scer\GAL4^ey.PH has lethal phenotype (Kurata et al., 2000)
Phenotype Manifest In
Enhanced by
Statement Reference Antp^Scer\UAS.cBa, N^act.sev, Scer\GAL4^ey.PH has eye phenotype, enhanceable by bip2^Scer\UAS.cPa, Scer\GAL4^ey.PH (Prince et al., 2008) Antp^Scer\UAS.cBa, N^act.sev, Scer\GAL4^ey.PH has wing \| ectopic phenotype, enhanceable by bip2^Scer\UAS.cPa, Scer\GAL4^ey.PH (Prince et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, enhanceable by bip2^Scer\UAS.cPa/Scer\GAL4^ey.PH (Prince et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has wing \| ectopic phenotype, enhanceable by bip2^Scer\UAS.cPa/Scer\GAL4^ey.PH (Prince et al., 2008)
Suppressed by
Statement Reference Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has antenna phenotype, suppressible by ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has arista phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has eye phenotype, suppressible by ey^Scer\UAS.cHa/Scer\GAL4^ey.PH (Plaza et al., 2001) Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has leg \| ectopic phenotype, suppressible by ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 has pretarsus \| ectopic phenotype, suppressible by pb^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^dpp.blk1 (Percival-Smith et al., 2005) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^Scer\UAS.cHa, Scer\GAL4^ey-OK107 (Plaza et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^{ΔCT.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008) Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by ey^{ΔPD.Scer\UAS}, Scer\GAL4^ey-OK107 (Plaza et al., 2008)
Suppressor of
Statement Reference Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 is a suppressor of eye \| ectopic phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Plaza et al., 2001)
Other
Statement Reference Antp^Scer\UAS.cBa, N^ICN.Scer\UAS, Scer\GAL4^ey.PH has head phenotype (Kurata et al., 2000, Kurata et al., 2000) Antp^Scer\UAS.cBa, N^ICN.Scer\UAS, Scer\GAL4^ey.PH has leg \| ectopic phenotype (Kurata et al., 2000) Antp^Scer\UAS.cBa, N^ICN.Scer\UAS, Scer\GAL4^ey.PH has leg phenotype (Kurata et al., 2000) Antp^Scer\UAS.cBa, N^ICN.Scer\UAS, Scer\GAL4^ey.PH has wing \| ectopic phenotype (Kurata et al., 2000, Kurata et al., 2000) Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has neuron & dorsal mesothoracic disc phenotype (Plaza et al., 2001, Plaza et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of ey[Scer\UAS.cHa] rescues eye formation in animals expressing Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107]. Co-expression of either ey[ΔPD.Scer\UAS] or ey[ΔCT.Scer\UAS] is able to rescue the eye loss caused by expression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107]. Animals co-expressing ey[ΔHD.Scer\UAS] and Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107] show lethality such that few pupae are obtained. The few escapers show partial rescue of the eye loss caused by expression of Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107]. (Plaza et al., 2008) Expression of Antp[Scer\UAS.cBa] and N[act.sev] under the control of Scer\GAL4[ey.PH] induces the ectopic outgrowth of T2-specific appendages (wings and legs) on the adult head the transformation of the anntenna into leg is not complete, owing to the fact that Scer\GAL4[ey.PH] is not expressed in the entire antennal disc. The penetrance of the eye-to-wing transformation found in Scer\GAL4[ey.PH]; Antp[Scer\UAS.cBa] mutants is increased upon ectopic co-expression of bip2[Scer\UAS.cPa] from 8% to 24%. Ectopic expression of bip2[Scer\UAS.cPa] in combination with Antp[Scer\UAS.cBa] and N[act.sev], under the control of Scer\GAL4[ey.PH] strongly enhances the penetrance of eye-to-wing transformation. About 80% of these flies show eye-to-wing transformation compared with 40% without co-expression of bip2[Scer\UAS.cPa]. (Prince et al., 2008) Co-expression of pb[Scer\UAS.T:Avic\GFP-EGFP] suppresses the arista-to-tarsus transformation seen in flies expressing Antp[Scer\UAS.cBa] under the control of Scer\GAL4[dpp.blk1]. (Percival-Smith et al., 2005) The antenna to leg transformation phenotype in Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 flies is suppressed by ey^Scer\UAS.cHa. Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^dpp.blk1 leads to a inhibition of neuronal differentiation in the wing disc. Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^ey.PH represses the Antp^Scer\UAS.cBa eye phenotypes. (Plaza et al., 2001) Expression of Antp^Scer\UAS.cBa and N^ICN.Scer\UAS driven by Scer\GAL4^ey.PH is lethal. About 1/4 of those few escapers that do survive are found to have ectopic wings on their heads that usually consisted of dorsal and ventral wing blades bordered by bristles of the wing margin, but lacking wing veins. About 17% of these flies showed ectopic leg structures induced by secondary transformation of the ectopic antennal tissue into leg structures, an additional 10% showed a transformation of the original antenna to leg structures. (Kurata et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Antp^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Bello (Bello, 2000.3.15, Kurata et al., 2000)
Secondary FlyBase IDs

References ( 9 )
Research paper	Plaza et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(36): 13439--13444 Cross-regulatory protein-protein interactions between Hox and Pax transcription factors. [FBrf0206999] Prince et al., 2008, Development 135(9): 1669--1679 The YPWM motif links Antennapedia to the basal transcriptional machinery. [FBrf0205311] Katsuyama et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(44): 15918--15923 Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. [FBrf0191445] Percival-Smith et al., 2005, Genome 48(4): 712--721 Tarsus determination in Drosophila melanogaster. [FBrf0187678] Alexandre and Vincent, 2003, Development 130(4): 729--739 Requirements for transcriptional repression and activation by Engrailed in Drosophila embryos. [FBrf0155721] Bello et al., 2003, Neuron 37(2): 209--219 A pulse of the Drosophila Hox protein Abdominal-A schedules the end of neural proliferation via neuroblast apoptosis. [FBrf0156017] Plaza et al., 2001, EMBO J. 20(4): 802--811 Molecular basis for the inhibition of Drosophila eye development by Antennapedia. [FBrf0134592] Kurata et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97(5): 2117--2122 Notch signaling and the determination of appendage identity. [FBrf0126718]
Personal communication to FlyBase	Bello, 2000.3.15, UAS-Antp construct. UAS-Antp construct. [FBrf0126721]

Allele Dmel\AntpScer\UAS.cBa

Allele Dmel\Antp^Scer\UAS.cBa