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General Information
Symbol
Dmel\p53259H.GUS
Species
D. melanogaster
Name
FlyBase ID
FBal0104953
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Construct: Full length coding region for p53 is expressed from both GMR and Scer\UAS regulatory sequences. Amino acid replacement: R259H.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of p53259H.GUS under the control of Scer\GAL4wor.PA induces neuroblast loss in larval brains. Many of the remaining neuroblasts exhibit necrosis markers, including elevated levels of calcium ions and the presence of ubiquitin-conjugated protein aggregates. No difference from controls is seen in the aggregation of mitochondria.

Expression of p53259H.GUS in adult flies under the control of Scer\GAL4Act5C.Switch.PR results in an increased median lifespan (by 8%) in females, but not male, flies. No increase in lifespan is observed when p53259H.GUS is expressed in larvae.

Scer\GAL4GMR.PF-driven overexpression of p53GUS.PB induces apoptosis in the developing eye disc, giving a rough eye phenotype that can be suppressed by co-expression of p53259H.GUS.

Flies that express p53259H.GUS, under the control of Scer\GAL4elav.Switch.PO, are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation than controls and Scer\GAL4elav.Switch.PO>p53259H.GUS females produce similar amounts of eggs to controls.

Expression of p53259H.GUS in adult neurons, driven by Scer\GAL4elav.Switch.PO after induction with RU486, leads to an increase in maximum lifespan.

Mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death.

When expression is driven by Scer\GAL4en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT Enhancer of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of fzyScer\UAS.cKa partially suppresses the neuroblast loss seen in larval brains expressing p53259H.GUS under the control of Scer\GAL4wor.PA.

Co-expression of p53259H.GUS does not suppress the defective eye phenotype caused by expression of Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU.

The wing phenotype of Scer\GAL4Act88F.PD, E2fScer\UAS.cNa is unaffected by co-expression of p53259H.GUS.

The rough eye phenotype of Scer\GAL4GMR.PF, E2fScer\UAS.cNa, DpScer\UAS.cDa flies is unaffected by co-expression of p53259H.GUS.

Expression of the dominant negative p53259H.GUS transgene, under the control of Scer\GAL4ey.PH, does not suppress the L2/+ eye phenotype.

Xenogenetic Interactions
Statement
Reference

The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C is largely suppressed by co-expression of p53259H.GUS.

Co-expression of p53259H.GUS does not rescue the autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E, but it does rescue the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid, transfected into S2 cells and used in transcription activation assays. Dominant negative mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
p53259H.GUS
Name Synonyms
Secondary FlyBase IDs
    References (18)