Allele Dmel\p53^259H.GUS

General Information
Symbol	Dmel\p53^259H.GUS	Species	D. melanogaster
Name		FlyBase ID	FBal0104953
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\p53

Allele class	gain of function
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| amino acid replacement
Nature of the Allele
Allele class	gain of function (Brodsky et al., 2000)
Mutagen	in vitro construct \| regulatory fusion (Brodsky et al., 2000) in vitro construct \| amino acid replacement (Brodsky et al., 2000)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length coding region for p53 is expressed from both GMR and Scer\UAS regulatory sequences. Amino acid replacement: R259H. (Brodsky et al., 2000)
Assay mode	Drosophila cell culture (Brodsky et al., 2000) In transgenic Drosophila (intraspecific) (Brodsky et al., 2000)
Carried in construct	P{GUS-p53.259H} (Sidorov et al., 2002, Sidorov and Belitskii, 2002, Jassim et al., 2003, Brodsky et al., 2000, Shcherbata et al., 2004, Bauer et al., 2005, Bauer et al., 2005, Singh et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	long lived \| conditional RU486, with Scer\GAL4^{elav.Switch.PO} (Bauer et al., 2005) cell death increase \| conditional qualifier (Jassim et al., 2003) cell death decrease, with Scer\GAL4^en-e16E (Brodsky et al., 2000) chemical resistant \| conditional RU486, with Scer\GAL4^{elav.Switch.PO} (Bauer et al., 2005)
Phenotype Manifest In
	eye \| conditional qualifier (Jassim et al., 2003) dorsal mesothoracic disc \| posterior compartment, with Scer\GAL4^en-e16E (Brodsky et al., 2000)
Detailed Description
	Statement Reference When expression is driven by Scer\GAL4^en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size. (Brodsky et al., 2000) Mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death. (Jassim et al., 2003) Expression of p53[259H.GUS] in adult neurons, driven by Scer\GAL4[elav.Switch.PO] after induction with RU486, leads to an increase in maximum lifespan. (Bauer et al., 2005) Flies that express p53[259H.GUS], under the control of Scer\GAL4[elav.Switch.PO], are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation than controls and Scer\GAL4[elav.Switch.PO]>p53[259H.GUS] females produce similar amounts of eggs to controls. (Bauer et al., 2005)
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of cell death increase \| larval stage \| dominant phenotype of L² (Singh et al., 2006)
Phenotype Manifest In
NOT Suppressor of
Statement Reference p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of eye disc \| ventral phenotype of L² (Singh et al., 2006) p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of eye \| ventral phenotype of L² (Singh et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Expression of the dominant negative p53[259H.GUS] transgene, under the control of Scer\GAL4[ey.PH], does not suppress the L[2]/+ eye phenotype. (Singh et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	6582 w¹¹¹⁸; P{GUS-p53.259H}3.1
Notes on Origin
Discoverer

Comments
	Carried in a plasmid, transfected into S2 cells and used in transcription activation assays. Dominant negative mutation. (Brodsky et al., 2000)
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	p53^259H.GUS
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Singh et al., 2006, Development 133(23): 4771--4781 Lobe and Serrate are required for cell survival during early eye development in Drosophila. [FBrf0192707] Bauer et al., 2005, Curr. Biol. 15(22): 2063--2068 Neuronal expression of p53 dominant-negative proteins in adult Drosophila melanogaster extends life span. [FBrf0189997] Shcherbata et al., 2004, Development 131(13): 3169--3181 The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. [FBrf0179442] Jassim et al., 2003, EMBO J. 22(20): 5622--5632 Dmp53 protects the Drosophila retina during a developmentally regulated DNA damage response. [FBrf0173083] Sidorov and Belitskii, 2002, Genetika, Moscow 38(7): 929--936 [The effect of transposon P(GUS.p53.259H)on the frequency of tumor clones in Drosophila melanogaster wtsp2/+ heterozygotes.] [FBrf0152051] Brodsky et al., 2000, Cell 101(1): 103--113 Drosophila p53 binds a damage response element at the reaper locus. [FBrf0127026]
Supplementary material	Bauer et al., 2005, Curr. Biol. 15(22): [FBrf0198673]
Abstract	Sidorov et al., 2002, A. Dros. Res. Conf. 43: 987C P{GUS&?p53.259H} raises carcinogen-induced wts tumor frequencies in wts/+ heterozygotes Drosophila melanogaster. [FBrf0145693]

Allele Dmel\p53259H.GUS

Allele Dmel\p53^259H.GUS