FlyBase Allele Report: Dmel\p53[259H.GUS]

General Information

Symbol

Dmel\p53^259H.GUS

Species

D. melanogaster

Name

FlyBase ID

FBal0104953

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{GUS-p53.259H}

Key Links

Transgenic product class

dominant_negative_variant

(Brodsky et al., 2000)

missense_variant

(Brodsky et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Brodsky et al., 2000)

missense_variant

(Brodsky et al., 2000)

Mutagen

in vitro construct

(Brodsky et al., 2000)

Progenitor genotype

Carried in construct

P{GUS-p53.259H}

Cytology

Description

UAS and GMR regulatory sequences are fused upstream of a mutated p53 coding sequence (carries a R259H amino acid replacement).

(Brodsky et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Sidorov and Belitskii, 2002)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Arctic.Aβ42.UAS.Tag:SS(rPENK)}

(Iijima et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | conditional

(Jassim et al., 2003)

neuroblast | larval stage, with Scer\GAL4^wor.PA

(Kuang et al., 2014)

wing disc posterior compartment, with Scer\GAL4^en-e16E

(Brodsky et al., 2000)

Detailed Description

Statement

Reference

Expression of p53^259H.GUS under the control of Scer\GAL4^wor.PA induces neuroblast loss in larval brains. Many of the remaining neuroblasts exhibit necrosis markers, including elevated levels of calcium ions and the presence of ubiquitin-conjugated protein aggregates. No difference from controls is seen in the aggregation of mitochondria.

(Kuang et al., 2014)

Expression of p53^259H.GUS in adult flies under the control of Scer\GAL4^{Act5C.Switch.PR} results in an increased median lifespan (by 8%) in females, but not male, flies. No increase in lifespan is observed when p53^259H.GUS is expressed in larvae.

(Shen et al., 2009)

Scer\GAL4^GMR.PF-driven overexpression of p53^GUS.PB induces apoptosis in the developing eye disc, giving a rough eye phenotype that can be suppressed by co-expression of p53^259H.GUS.

(Moon et al., 2008)

Flies that express p53^259H.GUS, under the control of Scer\GAL4^{elav.Switch.PO}, are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation than controls and Scer\GAL4^{elav.Switch.PO}>p53^259H.GUS females produce similar amounts of eggs to controls.

(Bauer et al., 2005)

Expression of p53^259H.GUS in adult neurons, driven by Scer\GAL4^{elav.Switch.PO} after induction with RU486, leads to an increase in maximum lifespan.

(Bauer et al., 2005)

Mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death.

(Jassim et al., 2003)

When expression is driven by Scer\GAL4^en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.

(Brodsky et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, p53^259H.GUS has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

NOT Enhancer of

Statement

Reference

p53^259H.GUS, Scer\GAL4^Act88F.PD is a non-enhancer of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008)

p53^259H.GUS, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

NOT Suppressor of

Statement

Reference

p53^259H.GUS/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

p53^259H.GUS/Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

p53^259H.GUS, Scer\GAL4^Act88F.PD is a non-suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008)

p53^259H.GUS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of increased cell death | dominant | larval stage phenotype of L²

(Singh et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, p53^259H.GUS has neuroblast | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

NOT Enhancer of

Statement

Reference

p53^259H.GUS, Scer\GAL4^Act88F.PD is a non-enhancer of wing pouch phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008)

p53^259H.GUS, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

NOT Suppressor of

Statement

Reference

p53^259H.GUS/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

p53^259H.GUS, Scer\GAL4^Act88F.PD is a non-suppressor of wing pouch phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008)

p53^259H.GUS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of eye disc | ventral phenotype of L²

(Singh et al., 2006)

p53^259H.GUS/Scer\GAL4^ey.PH is a non-suppressor of eye | ventral phenotype of L²

(Singh et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of fzy^Scer\UAS.cKa partially suppresses the neuroblast loss seen in larval brains expressing p53^259H.GUS under the control of Scer\GAL4^wor.PA.

(Kuang et al., 2014)

Co-expression of p53^259H.GUS does not suppress the defective eye phenotype caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

(Griswold et al., 2008)

The wing phenotype of Scer\GAL4^Act88F.PD, E2f^Scer\UAS.cNa is unaffected by co-expression of p53^259H.GUS.

The rough eye phenotype of Scer\GAL4^GMR.PF, E2f^Scer\UAS.cNa, Dp^Scer\UAS.cDa flies is unaffected by co-expression of p53^259H.GUS.

(Moon et al., 2008)

Expression of the dominant negative p53^259H.GUS transgene, under the control of Scer\GAL4^ey.PH, does not suppress the L²/+ eye phenotype.

(Singh et al., 2006)

Xenogenetic Interactions

Statement

Reference

The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E at 30[o]C is largely suppressed by co-expression of p53^259H.GUS.

Co-expression of p53^259H.GUS does not rescue the autonomous reduction in wing area caused by expression of Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E, but it does rescue the non-autonomous reduction in wing area caused by expression of Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E.

(Mesquita et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6582

w¹¹¹⁸; P{GUS-p53.259H}3.1

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in transcription activation assays. Dominant negative mutation.

(Brodsky et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dp53^259H

(Mesquita et al., 2010)

dp53^DN

(Mesquita et al., 2010)

p53^259H.GUS

p53^DN

(Nandakumar et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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