Expression of p53259H.GUS under the control of Scer\GAL4wor.PA induces neuroblast loss in larval brains. Many of the remaining neuroblasts exhibit necrosis markers, including elevated levels of calcium ions and the presence of ubiquitin-conjugated protein aggregates. No difference from controls is seen in the aggregation of mitochondria.
Expression of p53259H.GUS in adult flies under the control of Scer\GAL4Act5C.Switch.PR results in an increased median lifespan (by 8%) in females, but not male, flies. No increase in lifespan is observed when p53259H.GUS is expressed in larvae.
Scer\GAL4GMR.PF-driven overexpression of p53GUS.PB induces apoptosis in the developing eye disc, giving a rough eye phenotype that can be suppressed by co-expression of p53259H.GUS.
Mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death.
When expression is driven by Scer\GAL4en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.