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General Information
Symbol
Dmel\ShcBG
Species
D. melanogaster
Name
FlyBase ID
FBal0105104
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dshcBG
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    insertion site
    Comment:
    A two base (CC) insertion in codon 298 causes a frameshift and a premature stop at codon 337. Two nucleotide substitutions at codon 297 are also reported in Shc[BG}.
    point mutation
    Nucleotide change:
    ACA9428093AAT
    Reported nucleotide change:
    C?A
    Amino acid change:
    T297K | Shc-PA
    Reported amino acid change:
    T297N
    Comment:
    One of two nucleotide substitutions reported in ShcBG at codon 297. There is also an insertion of two bases at codon 298 causing a frameshift.
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Nucleotide substitution: C?A. Nucleotide substitution: A?T. Amino acid replacement: T297N. Above mutation followed by insertion of two C nucleotides, resulting in a frameshift at the N-terminal end of the SH2 domain and a subsequent stop codon at position 337.
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    ShcBG/ShcBG mushroom body clones have normal axon pruning.
    ShcBG homozygous clones in the dorsal air sac primordium are found at the tip of the primordium at a significantly lower frequency than wild-type clones.
    Embryos derived from homozygous female germ-line clones display terminal defects. Most embryos fail to hatch and show a reduction of posterior Filzkorper and the 8th abdominal segment as well as mild defects in the anterior head skeleton. Malpighian tubules are always absent. Embryos lacking both maternal and zygotic Shc function show defects in germband retraction and a variable reduction of ventral denticle belts not seen in paternally rescued embryos. 70% of homozygotes die during the late pupal stage. Females that survive to adulthood do not lay eggs. Homozygous adult survivors are delayed in development and show defects in the eye, wing and ovary. The eyes are reduced in size and slightly roughened. The severity of the phenotype is graded in an anterior to posterior fashion, with the anterior side being more disorganised than the posterior. The ommatidia in the posterior region of the eye appear essentially normal and contain the full complement of photoreceptors, whereas in the anterior part of the eye, several ommatidia lack one or more outer photoreceptors. These mutant ommatidia do contain small central R7-like photoreceptors. In some ommatidia, the photoreceptors show rotated planar polarity. There is a partially penetrant loss of wing vein material in the distal part of the L4 vein. Homozygous females contain mature oocytes surrounded by a weakly ventralised chorion (as indicated by the fusion of the dorsal appendages).
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    Statement
    Reference
    ShcBG/Shc[+] is a suppressor of visible | semidominant phenotype of EgfrE3
    NOT Suppressor of
    Statement
    Reference
    ShcBG is a non-suppressor of visible phenotype of sevS11.Tag:MYC
    Other
    Statement
    Reference
    Phenotype Manifest In
    NOT Enhancer of
    Statement
    Reference
    ShcBG is a non-enhancer of phenotype of Raf::tor12D.hs.sev
    ShcBG is a non-enhancer of phenotype of Ras85DV12.sev
    ShcBG is a non-enhancer of phenotype of rlSem
    Suppressor of
    Statement
    Reference
    ShcBG/Shc[+] is a suppressor of eye phenotype of EgfrE3
    ShcBG is a suppressor of abdominal 11 anal tuft phenotype of tor13D
    ShcBG is a suppressor of filzkorper phenotype of tor13D
    ShcBG/Shc[+] is a suppressor of wing phenotype of EgfrE3
    NOT Suppressor of
    Statement
    Reference
    ShcBG is a non-suppressor of phenotype of rlSem
    ShcBG is a non-suppressor of eye phenotype of sevS11.Tag:MYC
    ShcBG is a non-suppressor of ommatidium phenotype of sevS11.Tag:MYC
    ShcBG is a non-suppressor of phenotype of Raf::tor12D.hs.sev
    ShcBG is a non-suppressor of phenotype of Ras85DV12.sev
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Heterozygous females carrying tor13D produce embryos that produce very little cuticle. This phenotype is weakly suppressed if the females are carrying one copy of ShcBG; the embryos develop more cuticle and show posterior structures such as Filzkorper and Tuft structures. The phenotype is completely suppressed if the tor13D/+ females also carry homozygous ShcBG germline clones; embryos look normal and can hatch. The eye and wing phenotypes caused by EgfrE3 are dominantly suppressed by ShcBG. The EgfrE3 phenotype is completely suppressed if the flies are also homozygous for ShcBG and the viability of these flies is restored. Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 results in the formation of ectopic wing vein material at multiple sites on the wing. This phenotype is completely suppressed if the flies are also homozygous for ShcBG. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 produce strongly dorsalised eggs with no dorsal appendages. This phenotype is partially suppressed if the females are also homozygous for ShcBG; dorsal appendage material is produced around the anterior circumference of the egg. 85% of embryos derived from dosP115 ShcBG double mutant female germline clones lack all structures posterior to segment A7 and have reduced head skeletons. The remaining 15% retain segment A8 but no structures posterior to it. Embryos derived from ShcBG drkΔP24 double mutant female germline clones have a stronger phenotype than embryos derived from either single mutant female germline clone.
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Selected as: a dominant enhancer of the haploinsufficient dl mutant phenotype.
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (4)