FlyBase Allele Report: Scer\GAL4[UAS.cHa]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^UAS.cHa

General Information

Symbol

Scer\GAL4^UAS.cHa

Species

S. cerevisiae

Name

Saccharomyces cerevisiae UAS construct a of Hassan

FlyBase ID

FBal0105107

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

UAS-GAL4, GMH5, GMH5-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Hassan et al., 2000)

Progenitor genotype

Carried in construct

Cytology

Description

UAS regulatory sequences are fused upstream of a Scer\GAL4 cDNA.

(Hassan et al., 2000)

Allele components

Component

Use(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

The Bloomington Stock Center has discarded their stocks containing the P{UAS-Gal4.H} construct based on information received from Siqian Feng in Richard Mann's lab. Siqian sequenced the construct in the stocks held at Bloomington and found that the GAL4 coding region had not been cloned into the UAS vector in the proper orientation to produce GAL4 protein.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^slbo.2.6, sima^UAS.cBa

(Doronkin et al., 2010)

abnormal immune response, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.1-2

(Croker et al., 2007)

abnormal neuroanatomy, with Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa

(Bornstein et al., 2015)

abnormal oxidative stress response | adult stage | male limited, with HDAC1^JF01401, Scer\GAL4^tin.CΔ4

(Kopp et al., 2015)

abnormal stress response, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.1-2

(Akasaka et al., 2006)

abnormal stress response, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.3

(Akasaka et al., 2006)

decreased speed of aging, with Pten^UAS.cGb, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

decreased speed of aging, with Scer\GAL4^tin.cBa, foxo^TM.UAS

(Wessells et al., 2004)

decreased speed of aging, with Scer\GAL4^tin.cBa, foxo^UAS.cFa

(Wessells et al., 2004)

decreased speed of aging, with Scer\GAL4^tin.cBa, rpr^UAS.C

(Wessells et al., 2004)

increased speed of aging, with InR^UAS.cHa, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

long lived, with HDAC1^JF01401, Scer\GAL4^tin.CΔ4

(Kopp et al., 2015)

short lived, with Scer\GAL4^tin.cBa, Zzzz\CTG^250.UAS.cCa

(Chakraborty et al., 2015)

viable, with InR^UAS.cHa, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

viable, with Pten^UAS.cGb, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

viable, with Scer\GAL4^tin.cBa, foxo^TM.UAS

(Wessells et al., 2004)

viable, with Scer\GAL4^tin.cBa, foxo^UAS.cFa

(Wessells et al., 2004)

Phenotype Manifest In

adult heart, with Fatp1^GD1069, Scer\GAL4^tin.cBa

(Sujkowski et al., 2012)

adult heart, with HDAC1^JF01401, Scer\GAL4^tin.CΔ4

(Kopp et al., 2015)

adult heart, with InR^UAS.cHa, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

adult heart, with KCNQ^UAS.cOa, Scer\GAL4^tin.cBa

(Ocorr et al., 2007)

adult heart, with Pect^GD11689, Scer\GAL4^tin.cBa

(Lim et al., 2011)

adult heart, with Pten^UAS.cGb, Scer\GAL4^tin.cBa

(Wessells et al., 2004)

adult heart, with Scer\GAL4^tin.cBa, bbc^GD1980

(Lim et al., 2011)

adult heart, with Scer\GAL4^tin.cBa, foxo^TM.UAS

(Wessells et al., 2004)

adult heart, with Scer\GAL4^tin.cBa, foxo^UAS.cFa

(Wessells et al., 2004)

adult heart, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.1-2

(Akasaka et al., 2006)

adult heart, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.3

(Akasaka et al., 2006)

adult heart, with Scer\GAL4^tin.cBa, Sur^RNAi.UAS.4

(Akasaka et al., 2006)

adult heart, with Scer\GAL4^tin.cBa, Zzzz\CTG^250.UAS.cCa

(Chakraborty et al., 2015)

axon, with Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa

(Bornstein et al., 2015)

border follicle cell, with Scer\GAL4^slbo.2.6, sima^UAS.cBa

(Doronkin et al., 2010)

myofibril, with Scer\GAL4^tin.cBa, Zzzz\CTG^250.UAS.cCa

(Chakraborty et al., 2015)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

sli^UAS.cKa, Scer\GAL4^sim.PS, Scer\GAL4^UAS.cHa is a non-suppressor of abnormal neuroanatomy phenotype of ptc^unspecified

(Merianda et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

108491

y¹ w¹¹¹⁸; P{UAS-Gal4.H}3A

108492

y¹ w¹¹¹⁸; P{UAS-Gal4.H}12B

108493

P{UAS-Gal4.H}24, y¹ w¹¹¹⁸; P{UAS-Gal4.H}12B; P{UAS-Gal4.H}3A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^Scer\UAS.cHa

Scer\GAL4^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hassan

(Hassan et al., 2000)

Secondary FlyBase IDs

References (48)

Report Sections