FlyBase Allele Report: Dmel\Hem[C3-20]

General Information

Symbol

Dmel\Hem^C3-20

Species

D. melanogaster

Name

FlyBase ID

FBal0105158

Feature type

allele

Associated gene

Dmel\Hem

Associated Insertion(s)

Carried in Construct

Also Known As

kette^C3-020

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Zhu and Bhat, 2011, Hummel et al., 2000)

Mutagen

ethyl methanesulfonate

(Hummel et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: W256term.

(Hummel et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:22,287,221..22,287,221 [-]

Nucleotide change:

G22287221A

Amino acid change:

W256term | Hem-PA

Reported amino acid change:

W256term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Hummel et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration (with Df(3L)ED230)

(Zhu and Bhat, 2011)

abnormal cell migration (with Hem^J4-48)

(Zhu and Bhat, 2011)

abnormal cell migration | embryonic stage

(Zhu and Bhat, 2011)

abnormal neuroanatomy (with Df(3L)ED230)

(Zhu and Bhat, 2011)

abnormal neuroanatomy (with Hem^J4-48)

(Zhu and Bhat, 2011)

abnormal neuroanatomy | embryonic stage

(Zhu and Bhat, 2011)

Phenotype Manifest In

cytoskeleton

(Hummel et al., 2000)

cytoskeleton & embryonic epidermis | dorsal

(Hummel et al., 2000)

larval longitudinal connective

(Hummel et al., 2000)

larval RP2 motor neuron | embryonic stage

(Zhu and Bhat, 2011)

larval RP2 motor neuron | embryonic stage (with Df(3L)ED230)

(Zhu and Bhat, 2011)

larval RP2 motor neuron | embryonic stage (with Hem^J4-48)

(Zhu and Bhat, 2011)

larval ventral nerve cord commissure

(Bogdan and Klambt, 2003, Hummel et al., 2000)

midline glial cell

(Hummel et al., 2000)

Detailed Description

Statement

Reference

Approximately 8% of Hem^C3-20 mutant embryos display a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment.

Hem^J4-48/Hem^C3-20 mutant embryos have a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment.

Approximately 9% of Hem^C3-20/Df(3L)ED230 mutant embryos display a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment.

(Zhu and Bhat, 2011)

In Hem^C3-20 homozygous embryos the segmental commissures of the ventral nerve cord are not separated into distinct axon bundles and instead appear fused.

(Bogdan and Klambt, 2003)

Mutant embryos show fused commissures and disrupted longitudinal connectives. The midline glial cells migrate in an abnormal path; they migrate laterally away from the midline along the forming commissural bundles (in wild-type embryos the midline glial cells show a posterior-ward migration). In stage 16 embryos, the midline glial cells fail to intercalate between the segmental commissure and a fused commissure phenotype develops. In the dorsal epidermis, pronounced F-actin bundles are seen in homozygous embryos, which often have a wavy appearance. These pronounced bundles are not seen in wild-type embryos. Hem^Δ2-6/Hem^C3-20 animals almost never eclose. The axonal phenotype is more pronounced in Hem^Δ2-6/Hem^C3-20 larvae than in homozygous Hem^Δ2-6 larvae.

(Hummel et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Hem[+]/Hem^C3-20 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Abl⁴/Abl¹

(Lin et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hem^C3-20 has larval ventral nerve cord commissure phenotype, suppressible by SCAR[+]/SCAR^unspecified

(Bogdan and Klambt, 2003)

NOT suppressed by

Statement

Reference

Hem^C3-20 has larval ventral nerve cord commissure phenotype, non-suppressible by WASp³/WASp³

(Bogdan and Klambt, 2003)

NOT Suppressor of

Statement

Reference

Hem[+]/Hem^C3-20 is a non-suppressor of NMJ bouton | increased number phenotype of Abl⁴/Abl¹

(Lin et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton number per muscle area that is seen at the neuromuscular junction of Abl¹/Abl⁴ larvae is not suppressed by Hem^C3-20/+.

(Lin et al., 2009)

The fusion of commissures in the ventral nerve cords of Hem^C3-20 embryos is significantly repressed by SCAR^unspecified/+, but is unaffected by homozygosity for WASp³.

(Bogdan and Klambt, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hem⁰³³³⁵

(Hummel et al., 2000)

Rescued by

Hem^C3-20 is rescued by Scer\GAL4^rho.PL/Hem^{UAS.Tag:Myr(Src64B)}

(Bogdan and Klambt, 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8752

st¹ e¹ Hem^C3-20/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Allelic series based on mutant phenotype; Hem^C3-20 = Hem^J4-48 > Hem^P168 > Hem^G1-37 > Hem^J1-70.

(Hummel et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hem^C3-20

(Zhu and Bhat, 2011)

kette^C3-020

(Schroter et al., 2004, Hummel et al., 2000)

kette^C3-20

(Lin et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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