Allele Dmel\Hem^C3-20

General Information
Symbol	Dmel\Hem^C3-20	Species	D. melanogaster
Name		FlyBase ID	FBal0105158
Feature type	allele	Associated gene	Dmel\Hem
Also Known As	kette^C3-020

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Hummel et al., 2000, Zhu and Bhat, 2011)
Mutagen	ethyl methanesulfonate (Hummel et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:22,280,321..22,280,321 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=W256@\|Hem-PA reported_pr_change=W256@ na_change=G22280321A (Hummel et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W256@. (Hummel et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	cell migration defective (with Df(3L)ED230) (Zhu and Bhat, 2011) cell migration defective (with Hem^J4-48) (Zhu and Bhat, 2011) cell migration defective \| embryonic stage (Zhu and Bhat, 2011) neuroanatomy defective (with Df(3L)ED230) (Zhu and Bhat, 2011) neuroanatomy defective (with Hem^J4-48) (Zhu and Bhat, 2011) neuroanatomy defective \| embryonic stage (Zhu and Bhat, 2011)
Phenotype Manifest In
	cytoskeleton (Hummel et al., 2000) cytoskeleton & embryonic epidermis \| dorsal (Hummel et al., 2000) longitudinal connective (Hummel et al., 2000) midline glial cell (Hummel et al., 2000) RP2 motor neuron \| embryonic stage (Zhu and Bhat, 2011) RP2 motor neuron \| embryonic stage (with Df(3L)ED230) (Zhu and Bhat, 2011) RP2 motor neuron \| embryonic stage (with Hem^J4-48) (Zhu and Bhat, 2011) ventral nerve cord commissure (Hummel et al., 2000, Bogdan and Klambt, 2003)
Detailed Description
	Statement Reference Approximately 8% of Hem[C3-20] mutant embryos display a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment. Hem[J4-48]/Hem[C3-20] mutant embryos have a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment. Approximately 9% of Hem[C3-20]/Df(3L)ED230 mutant embryos display a duplication of the RP2 neuron in one hemisegment and have one missing in the contralateral segment. (Zhu and Bhat, 2011) In Hem^C3-20 homozygous embryos the segmental commissures of the ventral nerve cord are not separated into distinct axon bundles and instead appear fused. (Bogdan and Klambt, 2003) Mutant embryos show fused commissures and disrupted longitudinal connectives. The midline glial cells migrate in an abnormal path; they migrate laterally away from the midline along the forming commissural bundles (in wild-type embryos the midline glial cells show a posterior-ward migration). In stage 16 embryos, the midline glial cells fail to intercalate between the segmental commissure and a fused commissure phenotype develops. In the dorsal epidermis, pronounced F-actin bundles are seen in homozygous embryos, which often have a wavy appearance. These pronounced bundles are not seen in wild-type embryos. Hem^Δ2-6/Hem^C3-20 animals almost never eclose. The axonal phenotype is more pronounced in Hem^Δ2-6/Hem^C3-20 larvae than in homozygous Hem^Δ2-6 larvae. (Hummel et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference Hem[+]/Hem^C3-20 is a non-suppressor of neuroanatomy defective \| larval stage phenotype of Abl⁴/Abl¹ (Lin et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Hem^C3-20 has ventral nerve cord commissure phenotype, suppressible \| partially by SCAR[+]/SCAR^unspecified (Bogdan and Klambt, 2003)
NOT suppressed by
Statement Reference Hem^C3-20 has ventral nerve cord commissure phenotype, non-suppressible by WASp³/WASp³ (Bogdan and Klambt, 2003)
NOT Suppressor of
Statement Reference Hem[+]/Hem^C3-20 is a non-suppressor of NMJ bouton \| supernumerary phenotype of Abl⁴/Abl¹ (Lin et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference The increased bouton number per muscle area that is seen at the neuromuscular junction of Abl[1]/Abl[4] larvae is not suppressed by Hem[C3-20]/+. (Lin et al., 2009) The fusion of commissures in the ventral nerve cords of Hem^C3-20 embryos is significantly repressed by SCAR^unspecified/+, but is unaffected by homozygosity for WASp³. (Bogdan and Klambt, 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Hem⁰³³³⁵ (Hummel et al., 2000)
Rescued by	Hem^C3-20 is rescued by Scer\GAL4^rho.PL/Hem^{Scer\UAS.T:Myr-Src64B} (Bogdan and Klambt, 2003)
Comments
Stocks ( 1 )
Bloomington	8752 st¹ e¹ Hem^C3-20/TM6B, Tb¹
Notes on Origin
Discoverer

Comments
	Allelic series based on mutant phenotype; Hem^C3-20 = Hem^J4-48 > Hem^P168 > Hem^G1-37 > Hem^J1-70. (Hummel et al., 2000)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Hem^C3-20 (Zhu and Bhat, 2011) kette^C3-020 (Schroter et al., 2004, Hummel et al., 2000) kette^C3-20 (Lin et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Zhu and Bhat, 2011, Dev. Biol. 357(2): 283--294 The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase. [FBrf0214765] Lin et al., 2009, Development 136(18): 3099--3107 Abi plays an opposing role to Abl in Drosophila axonogenesis and synaptogenesis. [FBrf0208583] Schroter et al., 2004, Development 131(18): 4501--4509 kette and blown fuse interact genetically during the second fusion step of myogenesis in Drosophila. [FBrf0180145] Bogdan and Klambt, 2003, Development 130(18): 4427--4437 Kette regulates actin dynamics and genetically interacts with Wave and Wasp. [FBrf0162072] Hummel et al., 2000, Genes Dev. 14(7): 863--873 The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization. [FBrf0127130]

Allele Dmel\HemC3-20

Allele Dmel\Hem^C3-20