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General Information
Symbol
Dmel\DNApol-α180Emus304
Species
D. melanogaster
Name
FlyBase ID
FBal0105216
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
E(mus304)
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Loss of an "A" base in codon Q301 causes a frameshift and early translation termination 29 codons downstream.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Single base pair deletion in the third exon at codon 301, which results in a frameshift and a premature stop codon 29 codons downstream.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygotes show no increase in loss of heterozygosity compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has visible phenotype, suppressible by p535A-1-4/p535A-1-4

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has visible phenotype, suppressible | partially by CycB[+]/CycB2

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has visible phenotype, suppressible by CycAC8LR1/CycA[+]

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has increased cell death phenotype, suppressible by CycAC8LR1/CycA[+]

Enhancer of
Statement
Reference

E(mus304)[+]/DNApol-α180Emus304 is an enhancer of increased cell death phenotype of mei-4129D

E(mus304)[+]/DNApol-α180Emus304 is an enhancer of visible phenotype of Df(3L)W4/mus304D2

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has eye phenotype, suppressible by p535A-1-4/p535A-1-4

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has eye phenotype, suppressible | partially by CycB[+]/CycB2

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has eye phenotype, suppressible by CycAC8LR1/CycA[+]

E(mus304)[+]/DNApol-α180Emus304, mei-4129D has wing disc phenotype, suppressible by CycAC8LR1/CycA[+]

Enhancer of
Statement
Reference

E(mus304)[+]/DNApol-α180Emus304 is an enhancer of wing disc phenotype of mei-4129D

E(mus304)[+]/DNApol-α180Emus304 is an enhancer of macrochaeta phenotype of Df(3L)W4/mus304D2

Other
Additional Comments
Genetic Interactions
Statement
Reference

mei-4129D mutants that are also heterozygous for DNApol-α180Emus304 have a rough eye phenotype that includes fused ommatidia and tissue loss.

Heterozygosity for DNApol-α180Emus304 further increases the average number of apoptotic cells per larval wing disc is increased fourfold in mei-4129D/Y flies.

p535A-1-4/p535A-1-4 completely suppresses the rough eye phenotype of DNApol-α180Emus304/+ ; mei-4129D/Y flies. The increased level of apoptosis seen in DNApol-α180Emus304/+ ; mei-4129D/Y wing discs is restored by p535A-1-4/p535A-1-4 to the level seen in mei-4129D/Y single mutants.

Heterozygosity for DNApol-α180Emus304 further increases the increase in loss of heterozygosity seen in mei-4129D/Y flies. There is a significant increase in the frequency of mitotic crossovers in the e to st region of the third chromosome in the double mutants.

The rough eye phenotype seen in mei-4129D ; DNApol-α180Emus304/+ adults is partially rescued by CycB2/+ and completely rescued by CycAC8LR1/+. The increased apoptosis seen in the wing discs of mei-4129D ; DNApol-α180Emus304/+ mutants is rescued by CycAC8LR1/+ to levels indistinguishable from those of mei-4129D single mutants or mei-4129D ; CycAC8LR1/+ mutants.

The eyes of lok30 ; DNApol-α180Emus304/+ flies are indistinguishable from wild type. The wing discs of these animals show a slight increase in apoptosis compared to wild type.

grp209/grpZ5170 ; DNApol-α180Emus304/+ males have a rough eye phenotype, while grp209/grpZ5170 ; DNApol-α180Emus304/+ females have wild-type eyes. The wing discs of these mutant animals show an increase in apoptosis compared to wild type.

mus304D1/mus304D2 flies have wild-type eyes. If the flies are also heterozygous for E(mus304)1 they have rough eyes and abnormal or missing bristles. Homozygous mei-4129D flies have wild-type eyes. If the flies are also heterozygous for E(mus304)1 they have rough eyes and missing bristles. mus304D2/Df(3L)W4 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). The frequency of defects are increased by one copy of E(mus304)1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Separable from: mus304D2.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DNApol-α180Emus304
E(mus304)1
Name Synonyms
Secondary FlyBase IDs
    References (3)