p535A-1-4/p535A-1-4 completely suppresses the rough eye phenotype of DNApol-α180Emus304/+ ; mei-4129D/Y flies. The increased level of apoptosis seen in DNApol-α180Emus304/+ ; mei-4129D/Y wing discs is restored by p535A-1-4/p535A-1-4 to the level seen in mei-4129D/Y single mutants.
Heterozygosity for DNApol-α180Emus304 further increases the increase in loss of heterozygosity seen in mei-4129D/Y flies. There is a significant increase in the frequency of mitotic crossovers in the e to st region of the third chromosome in the double mutants.
The rough eye phenotype seen in mei-4129D ; DNApol-α180Emus304/+ adults is partially rescued by CycB2/+ and completely rescued by CycAC8LR1/+. The increased apoptosis seen in the wing discs of mei-4129D ; DNApol-α180Emus304/+ mutants is rescued by CycAC8LR1/+ to levels indistinguishable from those of mei-4129D single mutants or mei-4129D ; CycAC8LR1/+ mutants.
grp209/grpZ5170 ; DNApol-α180Emus304/+ males have a rough eye phenotype, while grp209/grpZ5170 ; DNApol-α180Emus304/+ females have wild-type eyes. The wing discs of these mutant animals show an increase in apoptosis compared to wild type.
mus304D1/mus304D2 flies have wild-type eyes. If the flies are also heterozygous for E(mus304)1 they have rough eyes and abnormal or missing bristles. Homozygous mei-4129D flies have wild-type eyes. If the flies are also heterozygous for E(mus304)1 they have rough eyes and missing bristles. mus304D2/Df(3L)W4 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). The frequency of defects are increased by one copy of E(mus304)1.