Allele Dmel\Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0105264
Feature type	allele	Associated gene	Dmel\Cdk7

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Leclerc et al., 2000) in vitro construct - coding region fusion (Leclerc et al., 2000) in vitro construct - regulatory fusion (Leclerc et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: D137A. Scer\UAS sequences drive a mutated Cdk7 cDNA tagged at the C terminus with T:Ivir\HA1. (Leclerc et al., 2000)
Carried in construct	P{UAS-Cdk7.T170A} (Leclerc et al., 2000)
Tagged with	T:Ivir\HA1 (Leclerc et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^Bx-MS1096 (Leclerc et al., 2000)
Phenotype Manifest In
	embryo, with Scer\GAL4^Kr.PM (Leclerc et al., 2000) mitotic cell cycle \| embryonic stage 1, with Scer\GAL4^Kr.PM (Leclerc et al., 2000) wing, with Scer\GAL4^Bx-MS1096 (Leclerc et al., 2000) wing vein, with Scer\GAL4^Bx-MS1096 (Leclerc et al., 2000)
Detailed Description
	Statement Reference When Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} is driven by Scer\GAL4^Bx-MS1096 malformations are induced in the wing. They range from alterations of the vein pattern to severe wing blade reduction. This phenotype is enhanced by the addition of Cdk7^null3. When Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} is expressed in early embryos under the control of Scer\GAL4^Kr.PM, at the end of the maternal division program, a corresponding zone with twice the normal density of interphase 14 nuclei are observed, resulting from the appearance of an extra synchronous division in the domain of expression of the Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} proteins. (Leclerc et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Kr.PM has mitotic cell cycle phenotype, enhanceable by twe^+t3.7 (Leclerc et al., 2000) Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Kr.PM has mitotic cell cycle phenotype, enhanceable by twe^+t10 (Leclerc et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference Extra copies of twe^+t3.7 or twe^+t10 or combinations thereof, enhance the mitotic cycle phenotype seen in Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} flies, when driven by Scer\GAL4^Kr.PM, in a dose dependant manner. (Leclerc et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} is rescued by Scer\GAL4^Bx-MS1096/Cdk7^{Scer\UAS.T:Ivir\HA1} (Leclerc et al., 2000)
Fails to rescue	Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Bx-MS1096 fails to rescue Cdk7^null3 (Leclerc et al., 2000)
Comments	Expression of Cdk7^{T170A.Scer\UAS.T:Ivir\HA1} by Scer\GAL4^Bx-MS1096 or under basal expression under the control of the Hsp70 promoter fails to rescue the Cdk7^null3 wing phenotypes. (Leclerc et al., 2000)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Cdk7^DN (Leclerc et al., 2000) Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Leclerc et al., 2000, EMBO J. 19(7): 1567--1575 Dominant-negative mutants reveal a role for the cdk7 kinase at the mid-blastula transition in Drosophila embryos. [FBrf0127187]

Allele Dmel\Cdk7T170A.Scer\UAS.T:Ivir\HA1

Allele Dmel\Cdk7^{T170A.Scer\UAS.T:Ivir\HA1}