Allele Dmel\ush^Scer\UAS.cFa

General Information
Symbol	Dmel\ush^Scer\UAS.cFa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Fossett	FlyBase ID	FBal0117270
Feature type	allele	Associated gene	Dmel\ush

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Fossett et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A full length ush cDNA is expressed under the control of Scer\UAS sequences. (Fossett et al., 2000)
Carried in construct	P{UAS-ush.F} (Singh et al., 2005, Muratoglu et al., 2006, Singh and Choi, 2003, Fossett et al., 2003, Fossett et al., 2000, Fossett et al., 2001, Sellin et al., 2006, Tokusumi et al., 2010, Kennell et al., 2012, Muratoglu et al., 2007, Tokusumi et al., 2007, Gajewski et al., 2007, Gao et al., 2009, Sorrentino et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number \| third instar larval stage, with Scer\GAL4^Cg.PA (Gao et al., 2009) partially lethal - majority die \| pupal stage, with Scer\GAL4^ey.PH (Fossett et al., 2001) planar polarity defective, with Scer\GAL4^ey.PH (Fossett et al., 2001) size defective \| adult stage (Kennell et al., 2012) viable, with Scer\GAL4^twi.PB (Muratoglu et al., 2006) visible, with Scer\GAL4^ey.PH (Fossett et al., 2001) visible \| adult stage (Kennell et al., 2012) visible \| heat sensitive, with Scer\GAL4^ey.PH (Singh and Choi, 2003)
Phenotype Manifest In
	antenna, with Scer\GAL4^ey.PH (Singh and Choi, 2003) antenna \| ectopic, with Scer\GAL4^ey.PH (Fossett et al., 2001) cardioblast, with Scer\GAL4^twi.PB (Fossett et al., 2000) crystal cell, with Scer\GAL4^lz-gal4 (Fossett et al., 2001) crystal cell, with Scer\GAL4^twi.PB (Fossett et al., 2003) embryonic/larval lymph gland, with Scer\GAL4^Mef2.PR (Muratoglu et al., 2006) embryonic/larval lymph gland, with Scer\GAL4^twi.PB (Muratoglu et al., 2006) eye, with Scer\GAL4^ey.PH (Singh and Choi, 2003, Fossett et al., 2001) eye disc \| heat sensitive, with Scer\GAL4^ey.PH (Singh et al., 2005) heart primordium, with Scer\GAL4^twi.PB (Fossett et al., 2001) ommatidium, with Scer\GAL4^ey.PH (Fossett et al., 2001) plasmatocyte \| third instar larval stage, with Scer\GAL4^Cg.PA (Gao et al., 2009) posterior signalling centre of anterior lymph gland pair, with Scer\GAL4^{kn-col85-GAL4} (Tokusumi et al., 2010)
Detailed Description
	Statement Reference Expression of ush[Scer\UAS.cFa] at 27[o]C in the fat body using Scer\GAL4[Cg.PA] results in flies that are proportionally smaller than control flies. No difference is seen in the pigmentation of abdominal segment A6. (Kennell et al., 2012) Expression of ush[Scer\UAS.cFa] in the posterior signaling center under the control of Scer\GAL4[kn-col85-GAL4] leads to defects in niche cell differentiation based on a lack of filopodia formation. (Tokusumi et al., 2010) Expression of ush[Scer\UAS.cFa] under the control of Scer\GAL4[Cg.PA] results in an almost complete lost of plasmatocytes in third instar larval lymph glands. Lymph gland size is normal in these animals. (Gao et al., 2009) Expression of ush^Scer\UAS.cFa, under the control of either Scer\GAL4^twi.PB or Scer\GAL4^Mef2.PR, results in impaired lymph gland development. (Muratoglu et al., 2006) Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC throughout development results in a very small eye field in the eye-antennal disc (in 80% of discs no eye field is seen), whereas the antennal field is relatively normal. (Singh et al., 2005) When ush^Scer\UAS.cFa is driven by Scer\GAL4^twi.PB a reduced number of crystal cells is seen in the mutant embryo. (Fossett et al., 2003) Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC results in adults that either lack eyes or have a small eye phenotype. Antennal development is also affected. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are maintained at 18^oC throughout embryogenesis and are then shifted to 29^oC results in elimination of the eye field without affecting antennal development. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are maintained at 29^oC throughout embryogenesis and are then shifted to 18^oC has a very subtle effect or no effect on eye development. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are shifted 29^oC during the early first larval instar and are then shifted back to 18^oC results in normal eye development. (Singh and Choi, 2003) When ush^Scer\UAS.cFa is driven by Scer\GAL4^lz-gal4 a significant decrease in the average number of crystal cells is seen, 30% at stage 13 embryos and 85% at stage 16 embryos. When ush^Scer\UAS.cFa is driven by Scer\GAL4^twi.PB a reduction in the umber of cardiac cells is seen in embryos. An average of 40% of embryos have gaps in the heart tube ranging from 10 cells missing to a complete absence of cardiac cells. When ush^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH severe defects are seen in the eye. These include ectopic production of antennae, reduction or loss of the eye, disrupted ommatidial polarity. This combination is also lethal for about 80% of the population before eclosion. (Fossett et al., 2001) Expression of ush^Scer\UAS.cFa driven by Scer\GAL4^twi.PB leads to a significant reduction in the number of cardial cells. Up to about 50% of cells are missing, with small gaps or large deletions present in the heart tubes. (Fossett et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference ush^Scer\UAS.cFa/Scer\GAL4^ey.PH is a suppressor \| heat sensitive of visible \| dominant phenotype of L² (Singh et al., 2005)
Other
Statement Reference L², Scer\GAL4^ey.PH, ush^Scer\UAS.cFa has lethal phenotype (Singh et al., 2005) L²/L[+], Scer\GAL4^ey.PH, ush^Scer\UAS.cFa has lethal phenotype (Singh et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^twi.PB, ush^Scer\UAS.cFa has crystal cell phenotype, enhanceable by srp^{NC.F.Scer\UAS}/Scer\GAL4^twi.PB (Fossett et al., 2003)
NOT Enhanced by
Statement Reference Scer\GAL4^twi.PB, ush^Scer\UAS.cFa has crystal cell phenotype, non-enhanceable by srp^{C.Scer\UAS.cHa}/Scer\GAL4^twi.PB (Fossett et al., 2003) Scer\GAL4^twi.PB, ush^Scer\UAS.cFa has crystal cell phenotype, non-enhanceable by srp^{NC.V421G.Scer\UAS}/Scer\GAL4^twi.PB (Fossett et al., 2003)
NOT suppressed by
Statement Reference Scer\GAL4^twi.PB, ush^Scer\UAS.cFa has crystal cell phenotype, non-suppressible by srp^{C.Scer\UAS.cHa}/Scer\GAL4^twi.PB (Fossett et al., 2003) Scer\GAL4^twi.PB, ush^Scer\UAS.cFa has crystal cell phenotype, non-suppressible by srp^{NC.V421G.Scer\UAS}/Scer\GAL4^twi.PB (Fossett et al., 2003)
Suppressor of
Statement Reference Scer\GAL4^Cg.PA/ush^Scer\UAS.cFa is a suppressor \| partially of lamellocyte phenotype of hop^Tum (Sorrentino et al., 2007) Scer\GAL4^twi.PB/ush^Scer\UAS.cFa is a suppressor of crystal cell \| ectopic phenotype of Scer\GAL4^twi.PB, srp^{NC.F.Scer\UAS} (Fossett et al., 2003) ush^Scer\UAS.cFa/Scer\GAL4^ey.PH is a suppressor \| heat sensitive of eye \| ventral phenotype of L² (Singh et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Lymph glands in hop[Tum]/Y animals expressing ush[Scer\UAS.cFa] under the control of Scer\GAL4[Cg.PA] are hypertrophic and produce lamellocytes, although the number of circulating lamellocytes is reduced by 90%. (Sorrentino et al., 2007) L²/+ increases the frequency of the no-eye phenotype seen in discs of animals expressing ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC throughout development from 80% to nearly 98% and the animals fail to eclose. The L²/+ phenotype of ventral eye loss is unaffected if expression of ush^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH at 29^oC from embryogenesis until the end of the first larval instar, or by Scer\GAL4^ey.PH at 29^oC from during the third larval instar. However, if ush^Scer\UAS.cFa expression is driven by Scer\GAL4^ey.PH at 29^oC during the second larval instar, the L²/+ ventral eye phenotype is significantly rescued. (Singh et al., 2005) The addition of srp^{NC.F.Scer\UAS} to ush^Scer\UAS.cFa, Scer\GAL4^twi.PB embryos enhances the loss of crystal phenotype seen in these animals. The addition of srp^{C.Scer\UAS.cHa} or srp^{NC.V421G.Scer\UAS} has no effect on the crystal cell phenotype. (Fossett et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ush^Scer\UAS.cFa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Fossett (Fossett et al., 2000)
Secondary FlyBase IDs

References ( 14 )
Research paper	Kennell et al., 2012, Dev. Dyn. 241(1): 161--168 The MicroRNA miR-8 is a positive regulator of pigmentation and eclosion in Drosophila. [FBrf0216982] Tokusumi et al., 2010, Development 137(21): 3561--3568 Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis. [FBrf0212074] Gao et al., 2009, Mol. Cell. Biol. 29(22): 6086--6096 Upregulation of the Drosophila friend of GATA Gene u-shaped by JAK/STAT signaling maintains lymph gland prohemocyte potency. [FBrf0208909] Gajewski et al., 2007, genesis 45(4): 200--207 Identification of a crystal cell-specific enhancer of the black cells prophenoloxidase gene in Drosophila. [FBrf0201838] Muratoglu et al., 2007, Dev. Biol. 311(2): 636--649 The GATA factor Serpent cross-regulates lozenge and u-shaped expression during Drosophila blood cell development. [FBrf0200604] Sorrentino et al., 2007, Dev. Biol. 311(2): 311--323 The Friend of GATA protein U-shaped functions as a hematopoietic tumor suppressor in Drosophila. [FBrf0201267] Tokusumi et al., 2007, Differentiation 75(2): 166--174 U-shaped protein domains required for repression of cardiac gene expression in Drosophila. [FBrf0191835] Muratoglu et al., 2006, Dev. Biol. 296(2): 561--579 Regulation of Drosophila friend of GATA gene, u-shaped, during hematopoiesis: A direct role for Serpent and Lozenge. [FBrf0195108] Sellin et al., 2006, Gene Expr. Patterns 6(4): 360--375 Dynamics of heart differentiation, visualized utilizing heart enhancer elements of the Drosophila melanogaster bHLH transcription factor Hand. [FBrf0190511] Singh et al., 2005, Genetics 171(1): 169--183 Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye. [FBrf0187660] Fossett et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(20): 11451--11456 Combinatorial interactions of serpent, lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis. [FBrf0167995] Singh and Choi, 2003, Development 130(25): 6351--6360 Initial state of the Drosophila eye before dorsoventral specification is equivalent to ventral. [FBrf0167519] Fossett et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(13): 7342--7347 The Friend of GATA proteins U-shaped, FOG-1, and FOG-2 function as negative regulators of blood, heart, and eye development in Drosophila. [FBrf0137102] Fossett et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97(13): 7348--7353 The multitype zinc-finger protein U-shaped functions in heart cell specification in the Drosophila embryo. [FBrf0128466]

Allele Dmel\ushScer\UAS.cFa

Allele Dmel\ush^Scer\UAS.cFa