|Name||Saccharomyces cerevisiae UAS construct a of Fossett||FlyBase ID||FBal0117270|
|Feature type||allele||Associated gene||Dmel\ush|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Singh et al., 2005, Muratoglu et al., 2006, Singh and Choi, 2003, Fossett et al., 2003, Fossett et al., 2000, Fossett et al., 2001, Sellin et al., 2006, Tokusumi et al., 2010, Kennell et al., 2012, Muratoglu et al., 2007, Tokusumi et al., 2007, Gajewski et al., 2007, Gao et al., 2009, Sorrentino et al., 2007)
|Phenotype Manifest In|
Expression of ush[Scer\UAS.cFa] at 27[o]C in the fat body using Scer\GAL4[Cg.PA] results in flies that are proportionally smaller than control flies. No difference is seen in the pigmentation of abdominal segment A6.
Expression of ush[Scer\UAS.cFa] in the posterior signaling center under the control of Scer\GAL4[kn-col85-GAL4] leads to defects in niche cell differentiation based on a lack of filopodia formation.
Expression of ush[Scer\UAS.cFa] under the control of Scer\GAL4[Cg.PA] results in an almost complete lost of plasmatocytes in third instar larval lymph glands. Lymph gland size is normal in these animals.
Expression of ushScer\UAS.cFa, under the control of either Scer\GAL4twi.PB or Scer\GAL4Mef2.PR, results in impaired lymph gland development.
Expression of ushScer\UAS.cFa under the control of Scer\GAL4ey.PH at 29oC throughout development results in a very small eye field in the eye-antennal disc (in 80% of discs no eye field is seen), whereas the antennal field is relatively normal.
When ushScer\UAS.cFa is driven by Scer\GAL4twi.PB a reduced number of crystal cells is seen in the mutant embryo.
Expression of ushScer\UAS.cFa under the control of Scer\GAL4ey.PH at 29oC results in adults that either lack eyes or have a small eye phenotype. Antennal development is also affected. Expression of ushScer\UAS.cFa under the control of Scer\GAL4ey.PH in animals that are maintained at 18oC throughout embryogenesis and are then shifted to 29oC results in elimination of the eye field without affecting antennal development. Expression of ushScer\UAS.cFa under the control of Scer\GAL4ey.PH in animals that are maintained at 29oC throughout embryogenesis and are then shifted to 18oC has a very subtle effect or no effect on eye development. Expression of ushScer\UAS.cFa under the control of Scer\GAL4ey.PH in animals that are shifted 29oC during the early first larval instar and are then shifted back to 18oC results in normal eye development.
When ushScer\UAS.cFa is driven by Scer\GAL4lz-gal4 a significant decrease in the average number of crystal cells is seen, 30% at stage 13 embryos and 85% at stage 16 embryos. When ushScer\UAS.cFa is driven by Scer\GAL4twi.PB a reduction in the umber of cardiac cells is seen in embryos. An average of 40% of embryos have gaps in the heart tube ranging from 10 cells missing to a complete absence of cardiac cells. When ushScer\UAS.cFa is driven by Scer\GAL4ey.PH severe defects are seen in the eye. These include ectopic production of antennae, reduction or loss of the eye, disrupted ommatidial polarity. This combination is also lethal for about 80% of the population before eclosion.
|Phenotype Manifest In|
|NOT Enhanced by|
Scer\GAL4twi.PB, ushScer\UAS.cFa has crystal cell phenotype, non-enhanceable by srpC.Scer\UAS.cHa/Scer\GAL4twi.PB
|NOT suppressed by|
Scer\GAL4twi.PB, ushScer\UAS.cFa has crystal cell phenotype, non-suppressible by srpC.Scer\UAS.cHa/Scer\GAL4twi.PB
Scer\GAL4twi.PB/ushScer\UAS.cFa is a suppressor of crystal cell | ectopic phenotype of Scer\GAL4twi.PB, srpNC.F.Scer\UAS
Lymph glands in hop[Tum]/Y animals expressing ush[Scer\UAS.cFa] under the control of Scer\GAL4[Cg.PA] are hypertrophic and produce lamellocytes, although the number of circulating lamellocytes is reduced by 90%.
L2/+ increases the frequency of the no-eye phenotype seen in discs of animals expressing ushScer\UAS.cFa under the control of Scer\GAL4ey.PH at 29oC throughout development from 80% to nearly 98% and the animals fail to eclose. The L2/+ phenotype of ventral eye loss is unaffected if expression of ushScer\UAS.cFa is driven by Scer\GAL4ey.PH at 29oC from embryogenesis until the end of the first larval instar, or by Scer\GAL4ey.PH at 29oC from during the third larval instar. However, if ushScer\UAS.cFa expression is driven by Scer\GAL4ey.PH at 29oC during the second larval instar, the L2/+ ventral eye phenotype is significantly rescued.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Fossett
|Secondary FlyBase IDs|
|References ( 14 )|