Allele Dmel\trio^Scer\UAS.cBa

General Information
Symbol	Dmel\trio^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Bateman	FlyBase ID	FBal0117278
Feature type	allele	Associated gene	Dmel\trio

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bateman et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length wild type trio cDNA is expressed under the influence of Scer\UAS regulatory sequences. (Bateman et al., 2000)
Carried in construct	P{UAS-trio.B} (Geisbrecht and Montell, 2004, Bateman et al., 2000, Astigarraga et al., 2010, Lee and Thomas, 2011, Song and Giniger, 2011, Malartre et al., 2010, Ball et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PFa (Lee and Thomas, 2011) ommatidium, with Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
Detailed Description
	Statement Reference Overexpression of a single copy of trio[Scer\UAS.cBa] during eye development driven by Scer\GAL4[GMR.PFa] causes a mild rough-eye phenotype, and expression of two copies results in a reduced number of ommatidia and a smaller bar shaped eye. (Lee and Thomas, 2011) Pan-neuronal expression of trio[Scer\UAS.cBa] under the control of Scer\GAL4[elav-C155] does not produce any dominant axon patterning defects in a wild-type background. (Song and Giniger, 2011) Third instar larvae expressing trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] show a similar number of boutons per muscle surface area as in wild type. (Ball et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has visible phenotype, enhanceable by kst[+]/kst¹ (Lee and Thomas, 2011) Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has visible phenotype, enhanceable by kst[+]/kst² (Lee and Thomas, 2011) Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has visible phenotype, enhanceable by kst[+]/kst^B1-14.1 (Lee and Thomas, 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has visible phenotype, non-enhanceable by kst^Δ.Scer\UAS/Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has visible phenotype, non-suppressible by kst^Δ.Scer\UAS/Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
Enhancer of
Statement Reference Scer\GAL4^elav-C155/trio^Scer\UAS.cBa is an enhancer of neuroanatomy defective \| heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+] (Song and Giniger, 2011) trio^Scer\UAS.cBa/Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Scer\GAL4^GMR.PFa, kst^GD1472 (Lee and Thomas, 2011)
Suppressor of
Statement Reference Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of neuroanatomy defective \| third instar larval stage phenotype of Mad^k00237/Mad¹ (Ball et al., 2010) Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of neuroanatomy defective \| third instar larval stage phenotype of wit^HA3/wit^A12 (Ball et al., 2010) trio^Scer\UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of visible phenotype of Scer\GAL4^ey.200.Exel, kst^{βH33.Scer\UAS} (Lee and Thomas, 2011) trio^Scer\UAS.cBa/Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Scer\GAL4^GMR.PFa, kst^{βH33.Scer\UAS} (Lee and Thomas, 2011)
NOT Suppressor of
Statement Reference trio^Scer\UAS.cBa/Scer\GAL4^arm.PS is a non-suppressor of partially lethal - majority die \| male limited \| pupal stage phenotype of Vav² (Malartre et al., 2010) trio^Scer\UAS.cBa/Scer\GAL4^arm.PS is a non-suppressor of partially lethal - majority die phenotype of Vav³ (Malartre et al., 2010) trio^Scer\UAS.cBa/Scer\GAL4^αTub84B.PL is a non-suppressor of partially lethal - majority die \| male limited \| pupal stage phenotype of Vav² (Malartre et al., 2010) trio^Scer\UAS.cBa/Scer\GAL4^αTub84B.PL is a non-suppressor of partially lethal - majority die phenotype of Vav³ (Malartre et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has eye phenotype, enhanceable by kst[+]/kst¹ (Lee and Thomas, 2011) Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has eye phenotype, enhanceable by kst[+]/kst² (Lee and Thomas, 2011) Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has eye phenotype, enhanceable by kst[+]/kst^B1-14.1 (Lee and Thomas, 2011)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has eye phenotype, non-enhanceable by kst^Δ.Scer\UAS/Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PFa, trio^Scer\UAS.cBa has eye phenotype, non-suppressible by kst^Δ.Scer\UAS/Scer\GAL4^GMR.PFa (Lee and Thomas, 2011)
Enhancer of
Statement Reference Scer\GAL4^elav-C155/trio^Scer\UAS.cBa is an enhancer of intersegmental nerve \| heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+] (Song and Giniger, 2011) Scer\GAL4^elav-C155/trio^Scer\UAS.cBa is an enhancer of intersegmental nerve branch ISNb of A1-7 \| heat sensitive phenotype of N^l1N-ts1, trio^123.4/trio[+] (Song and Giniger, 2011) trio^Scer\UAS.cBa/Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, kst^GD1472 (Lee and Thomas, 2011)
Suppressor of
Statement Reference Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Mad^k00237/Mad¹ (Ball et al., 2010) Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of wit^HA3/wit^A12 (Ball et al., 2010) Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of NMJ bouton \| third instar larval stage phenotype of Mad^k00237/Mad¹ (Ball et al., 2010) Scer\GAL4^BG380/trio^Scer\UAS.cBa is a suppressor \| partially of NMJ bouton \| third instar larval stage phenotype of wit^HA3/wit^A12 (Ball et al., 2010) Scer\GAL4^elav-C155/trio^Scer\UAS.cBa is a suppressor of NMJ bouton \| larval stage phenotype of Liprin-α^oos (Astigarraga et al., 2010) Scer\GAL4^elav-C155/trio^Scer\UAS.cBa is a suppressor of NMJ bouton \| larval stage phenotype of Liprin-β^Δ83 (Astigarraga et al., 2010) Scer\GAL4^how-24B, trio^Scer\UAS.cBa, Scer\GAL4^how-24B is a suppressor of NMJ bouton \| larval stage phenotype of Liprin-β^Δ83 (Astigarraga et al., 2010) trio^Scer\UAS.cBa, Scer\GAL4^elav-C155 is a suppressor of intersegmental nerve phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^elav-C155 (Bateman et al., 2000) trio^Scer\UAS.cBa, Scer\GAL4^slbo.2.6 is a suppressor \| partially of border follicle cell phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2004) trio^Scer\UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, kst^{βH33.Scer\UAS} (Lee and Thomas, 2011) trio^Scer\UAS.cBa/Scer\GAL4^ey.200.Exel is a suppressor of macrochaeta \| ectopic phenotype of Scer\GAL4^ey.200.Exel, kst^{βH33.Scer\UAS} (Lee and Thomas, 2011) trio^Scer\UAS.cBa/Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PFa, kst^{βH33.Scer\UAS} (Lee and Thomas, 2011)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for kst[1] significantly enhances the rough-eye phenotype induced by overexpression of trio[Scer\UAS.cBa] via Scer\GAL4[GMR.PFa]. Heterozygosity for kst[2] significantly enhances the rough-eye phenotype induced by overexpression of trio[Scer\UAS.cBa] via Scer\GAL4[GMR.PFa]. Heterozygosity for kst[B1-14.1] significantly enhances the rough-eye phenotype induced by overexpression of trio[Scer\UAS.cBa] via Scer\GAL4[GMR.PFa]. Co-expression of kst[Δ.Scer\UAS] with trio[Scer\UAS.cBa] under the control of Scer\GAL4[GMR.PFa] does not modify the eye phenotype of trio[Scer\UAS.cBa]-overexpression alone. Co-expression of kst[βH33.Scer\UAS] and trio[Scer\UAS.cBa] under the control of Scer\GAL4[GMR.PFa] suppresses the roughness of both the kst[βH33.Scer\UAS] and the trio[Scer\UAS.cBa] eye phenotype. Co-expression of trio[Scer\UAS.cBa] with kst[βH33.Scer\UAS] under the control of Scer\GAL4[ey.200.Exel] suppresses the eye phenotype resulting from the overexpression of kst[βH33.Scer\UAS]. (Lee and Thomas, 2011) A trio[123.4] heterozygous background significantly restores the ability of ISNb axons to enter the ventrolateral muscle field in N[l1N-ts1] mutants. This suppression is reverted upon pan-neural expression of trio[Scer\UAS.cBa] under the control of Scer\GAL4[elav-C155]. (Song and Giniger, 2011) Scer\GAL4[how-24B]- or Scer\GAL4[elav-C155]-mediated expression of trio[Scer\UAS.cBa] restores the number of boutons in Liprin-β[Δ83] animals to control levels. Scer\GAL4[elav-C155]-mediated expression of trio[Scer\UAS.cBa] restores the number of boutons in Liprin-α[oos] animals to control levels. (Astigarraga et al., 2010) Expression of trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] partially suppresses the reduced bouton number phenotype seen in Mad[1]/Mad[k00237] larval neuromuscular junctions. Expression of trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] partially suppresses the reduced bouton number phenotype seen in wit[A12]/wit[HA3] larval neuromuscular junctions. (Ball et al., 2010) Expression of trio[Scer\UAS.cBa] under the control of either the Scer\GAL4[arm.PS] or the Scer\GAL4[αTub84B.PL] driver cannot suppress the semi-lethality seen in vav[2] male homozygotes. Expression of trio[Scer\UAS.cBa] under the control of either the Scer\GAL4[arm.PS] or the Scer\GAL4[αTub84B.PL] driver cannot suppress the semi-lethality seen in vav[3] male homozygotes. (Malartre et al., 2010) The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is partially suppressed by coexpression of trio^Scer\UAS.cBa. (Geisbrecht and Montell, 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^{elav.Switch.PO}/trio^Scer\UAS.cBa rescues trio^6A/trio^S137203 (Ball et al., 2010) trio^Scer\UAS.cBa/Scer\GAL4^Rapgap1-OK6 rescues trio^6A/trio^S137203 (Ball et al., 2010)
Partially rescues	Scer\GAL4^elav-C155/trio^Scer\UAS.cBa partially rescues trio^6A/Df(3L)Ar12-1 (Bateman et al., 2000) Scer\GAL4^elav-C155/trio^Scer\UAS.cBa partially rescues trio^S137203/trio^BX1B (Bateman et al., 2000) Scer\GAL4^T155/trio^Scer\UAS.cBa partially rescues trio^S138606/Df(3L)Ar12-1 (Bateman et al., 2000)
Fails to rescue	Scer\GAL4^G14/trio^Scer\UAS.cBa fails to rescue trio^6A/trio^S137203 (Ball et al., 2010)
Comments	Expression of trio[Scer\UAS.cBa] in neurons under the control of Scer\GAL4[OK6] rescues the reduction in synaptic bouton phenotype seen in trio[S137203]/trio[6A] third instar larvae. Expression of trio[Scer\UAS.cBa] in muscles using Scer\GAL4[G14] is unable to rescue the phenotype. Expression of trio[Scer\UAS.cBa] only in the larval stages using the neuronal driver Scer\GAL4[elav.Switch.PO] rescues the reduction in synaptic bouton phenotype seen in trio[S137203]/trio[6A] third instar larvae. (Ball et al., 2010) When expression is driven by Scer\GAL4^T155, wing posture of trio^S138606/Df(3L)Ar12-1 is substantially rescued. When expression is driven by Scer\GAL4^elav-C155, larval lethality is rescued to pupal lethality. When expression is driven by Scer\GAL4^elav-C155, short stop phenotype of ISNb is reduced and short stop phenotype of SNa is eliminated. (Bateman et al., 2000)
Stocks ( 2 )
Bloomington	9513 w^; P{UAS-trio.B}2B 9512 P{UAS-trio.B}1B, y¹ w^
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	trio^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Bateman (Bateman et al., 2000)
Secondary FlyBase IDs

References ( 7 )
Research paper	Lee and Thomas, 2011, Mech. Dev. 128(1-2): 116--128 Rac1 modulation of the apical domain is negatively regulated by β(Heavy)-spectrin. [FBrf0213021] Song and Giniger, 2011, Dev. Dyn. 240(2): 324--332 Noncanonical notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of trio. [FBrf0212819] Astigarraga et al., 2010, J. Neurosci. 30(46): 15358--15368 Three Drosophila liprins interact to control synapse formation. [FBrf0212351] Ball et al., 2010, Neuron 66(4): 536--549 Retrograde BMP Signaling Controls Synaptic Growth at the NMJ by Regulating Trio Expression in Motor Neurons. [FBrf0210920] Malartre et al., 2010, J. Neurosci. 30(6): 2257--2267 The Guanine Exchange Factor vav Controls Axon Growth and Guidance during Drosophila Development. [FBrf0209937] Geisbrecht and Montell, 2004, Cell 118(1): 111--125 A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migration. [FBrf0179220] Bateman et al., 2000, Neuron 26(1): 93--106 The guanine nucleotide exchange factor trio mediates axonal development in the Drosophila embryo. [FBrf0127003]

Allele Dmel\trioScer\UAS.cBa

Allele Dmel\trio^Scer\UAS.cBa