FlyBase Allele Report: Dmel\trio[S137203]

General Information

Symbol

Dmel\trio^S137203

Species

D. melanogaster

Name

FlyBase ID

FBal0117281

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

P{lacW}trio^S137203

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ball et al., 2010)

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}trio^S137203

Cytology

Description

P{lacW} in the first exon of the trio transcript, in the untranslated region. P{lacW}trio^S137203 and P{lacW}trio^S138606 map within 5bp of each other.

(Bateman et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:1,034,856..1,034,856 [-]

(Bateman et al., 2000, Deak et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Ball et al., 2010)

abnormal neuroanatomy (with Df(3L)Ar12-1)

(Ball et al., 2010)

abnormal neuroanatomy (with trio^6A)

(Ball et al., 2010)

partially lethal - majority die | recessive

(Bateman et al., 2000, Deak et al., 1997)

visible | recessive

(Bateman et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous trio^S137203 third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls.

trio^S137203/Df(3L)Ar12-1 third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls.

trio^S137203/trio^6A third instar larvae show a reduction in the number of boutons per muscle surface area (MSA). The number of boutons per MSA at muscle 4 is approximately 35% less than in controls. The sizes of evoked excitatory junctional potentials (EJPs), miniature excitatory junctional potentials and the quantal content do not differ significantly from controls.

(Ball et al., 2010)

In heterozygous mutants, an occasional loss of the RP3 neuron is seen.

(Hu et al., 2001)

Adult escapers have a wing posture phenotype. Mutants show a short stop phenotype, with defects in the ability of nerve branches to reach target muscles. ISNb (where the effect is most pronounced) and SNa are most sensitive to the loss of trio activity. In addition, there are occasional defects in epidermal muscle attachment sites. The CNS also shows pathfinding errors resulting in breaks in the longitudinal connectives. The axons often fail to traverse the segment boundary.

(Bateman et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

trio^S137203 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Mad^k00237

(Ball et al., 2010)

Suppressor of

Statement

Reference

trio^S137203 is a suppressor of abnormal size | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^S137203 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^6A/trio^S137203 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^6A/trio^S137203 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^BG380, sax^A.UAS.cBa, tkv^{Q199D.UAS.Tag:HA}

(Ball et al., 2010)

trio^S137203/trio[+] is a suppressor | partially of abnormal size | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^S137203/trio[+] is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

trio^6A/trio^S137203 is a non-suppressor of abnormal size | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

trio^6A/trio^S137203 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

trio^S137203 is an enhancer of larval VL3/4 motor neuron phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

trio^S137203 is an enhancer of larval intersegmental nerve phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

trio^S137203/trio[+] is an enhancer of phenotype of Lar^5.5

(Bateman et al., 2000)

trio^S137203/trio[+] is an enhancer of larval intersegmental nerve phenotype of Lar^5.5

(Bateman et al., 2000)

NOT Enhancer of

Statement

Reference

trio^S137203 is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Mad^k00237

(Ball et al., 2010)

trio^S137203 is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Mad^k00237

(Ball et al., 2010)

Suppressor of

Statement

Reference

trio^S137203/trio[+] is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^S137203/trio[+] is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^S137203 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^S137203 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

NOT Suppressor of

Statement

Reference

trio^6A/trio^S137203 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

trio^6A/trio^S137203 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of trio^S137203 partially suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4^OK6. Bouton number is reduced by more than 50%.

Homozygous trio^S137203 suppresses the larval neuromuscular junction overgrowth and increase in bouton number seen when Rac1^Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4^OK6.

trio^S137203/trio^6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1^Scer\UAS.cLa is expressed in neurons under the control of Scer\GAL4^OK6.

trio^S137203 does not enhance the reduction in bouton number phenotype seen in Mad^k00237 larval neuromuscular junctions.

trio^S137203/trio^6A does not suppress the larval neuromuscular junction overgrowth and increase in bouton number seen when Rac1^V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4^BG380.

trio^S137203/trio^6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when sax^{A.Scer\UAS.cBa} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1} are expressed in motor neurons under the control of Scer\GAL4^BG380.

(Ball et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

trio^6A/trio^S137203 is rescued by trio^UAS.cBa/Scer\GAL4^RapGAP1-OK6

(Ball et al., 2010)

trio^6A/trio^S137203 is rescued by Scer\GAL4^{elav.Switch.PO}/trio^UAS.cBa

(Ball et al., 2010)

Partially rescued by

trio^S137203/trio^BX1B is partially rescued by Scer\GAL4^elav-C155/trio^UAS.cBa

(Bateman et al., 2000)

Not rescued by

trio^6A/trio^S137203 is not rescued by Scer\GAL4^G14/trio^UAS.cBa

(Ball et al., 2010)

Comments

Expression of trio^Scer\UAS.cBa in neurons under the control of Scer\GAL4^OK6 rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae. Expression of trio^Scer\UAS.cBa in muscles using Scer\GAL4^G14 is unable to rescue the phenotype.

Expression of trio^Scer\UAS.cBa only in the larval stages using the neuronal driver Scer\GAL4^{elav.Switch.PO} rescues the reduction in synaptic bouton phenotype seen in trio^S137203/trio^6A third instar larvae.

(Ball et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8594

w^*; P{lacW}trio^S137203/TM6B, Tb⁺

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} restores viability and reverts the wing phenotype.

(Bateman et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

1372/03

(Deak et al., 1997)

Trio^1372/3

(Hu et al., 2001)

l(3)S137203

(Saito et al., 2002)

trio^S137203

(Spinner et al., 2018, Long et al., 2016, Choi et al., 2014, Ball et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

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