FlyBase Allele Report: Dmel\Adcy1[UAS.cZa]

General Information

Symbol

Dmel\Adcy1^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zars

FlyBase ID

FBal0117384

Feature type

allele

Associated gene

Dmel\Adcy1

Associated Insertion(s)

Carried in Construct

P{UAS-Adcy1.Z}

Also Known As

UAS-rut, UAS-rut⁺, UAS-rutabaga

Key Links

Transgenic product class

cDNA

(Zars et al., 2000)

wild_type

(Zars et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zars et al., 2000)

wild_type

(Zars et al., 2000)

Mutagen

in vitro construct

(McGuire et al., 2003, Zars et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Adcy1.Z}

Cytology

Description

Expression of rut[+] cDNA is governed by UAS regulatory sequences.

(Zars et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Adcy1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^eve.RN2

(Baines, 2004)

abnormal neurophysiology, with Scer\GAL4^eve.RN2O

(Baines, 2003)

Phenotype Manifest In

larval DA1 motor neuron, with Scer\GAL4^eve.RN2O

(Baines, 2003)

larval RP2 motor neuron, with Scer\GAL4^eve.RN2O

(Baines, 2003)

Detailed Description

Statement

Reference

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf does not significantly alter the response of the M pacemaker neurons in the brain to Pdf.

(Duvall and Taghert, 2012)

Postsynaptic expression of rut^UAS.cZa, under the control of Scer\GAL4^eve.RN2, results in the averaged amplitude of synaptic currents recorded from whole-cell voltage clamp recordings of aCC/RP2 motoneurons being significantly increased compared with controls. The overall frequency of individual synaptic currents is not significantly different. In contrast, expression of rut^UAS.cZa in presynaptic cholinergic interneurons, driven by Scer\GAL4^ChAT.7.4, does not affect the averaged synaptic current amplitude.

(Baines, 2004)

The ability of aCC/RP2 to fire action potentials (APs) in response to an injection of constant current is decreased and the amount of current required to fire an equal number of APs is increased in mutants that express rut^Scer\UAS.cZa under the control of Scer\GAL4^eve.RN2O compared to wild type. There is also a decrease in peak current density of voltage-dependent inward Na⁺ current compared to wild type.

(Baines, 2003)

Wild-type flies over-expressing rut^Scer\UAS.cZa under the control of Scer\GAL4²⁴⁷ show a small (~25%) memory decay over 3 hours, as in wild-type.

(Schwaerzel et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Adcy1^UAS.cZa, Scer\GAL4^eve.RN2 has abnormal neurophysiology phenotype, suppressible by gbb¹

(Baines, 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of decreased body size | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of decreased size | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^eve.RN2/Adcy1^UAS.cZa is a non-suppressor of abnormal neurophysiology | recessive phenotype of gbb¹

(Baines, 2004)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of muscle cell of A1-7 ventral longitudinal muscle 3 | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Smn^Δ

(Li et al., 2020)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa is a non-suppressor of NMJ bouton | larval stage | decreased number phenotype of Smn^Δ

(Li et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^eve.RN2 fails to potentiate synaptic current amplitude in a gbb¹ background.

(Baines, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ey-OK107/Adcy1^UAS.cZa rescues Adcy1^rut-1

(Scheunemann et al., 2012)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa rescues Adcy1^rut-1

(Scheunemann et al., 2012)

Scer\GAL4^Tdc2.PC/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Koon et al., 2011)

Adcy1^UAS.cZa/Scer\GAL4^P2.4.Pdf rescues Adcy1^rut-2080

(Donlea et al., 2009)

Scer\GAL4^{elav.Switch.PO}/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Donlea et al., 2009)

Scer\GAL4^Mz717/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Donlea et al., 2009)

Scer\GAL4^121Y/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Liu et al., 2006)

Adcy1^UAS.cZa/Scer\GAL4^elav.PLu rescues Adcy1^rut-2080

(Liu et al., 2006)

Scer\GAL4^104Y/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Liu et al., 2006)

Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Blow et al., 2005)

Scer\GAL4^{Mef2.247.Switch}/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Mao et al., 2004)

Scer\GAL4^{Mef2.247.Switch}/Adcy1^UAS.cZa rescues Adcy1^MB2769

(Mao et al., 2004)

Scer\GAL4^c747/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(McGuire et al., 2003)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(McGuire et al., 2003)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Schwaerzel et al., 2002)

Scer\GAL4^c271/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^c522/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^c747/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Adcy1^UAS.cZa/Scer\GAL4^elav.PLu rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^238Y/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^30Y/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^H24/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^c747/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa rescues Adcy1^rut-2080

(Zars et al., 2000)

Partially rescues

Scer\GAL4^5-HT1B.PY/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Xie et al., 2013)

Scer\GAL4^Tab2-201Y/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^ey-OK107/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^c309/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^NP6510/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Liu et al., 2006)

Adcy1^UAS.cZa/Scer\GAL4^NP6561 partially rescues Adcy1^rut-2080

(Liu et al., 2006)

Scer\GAL4^c205/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Liu et al., 2006)

Scer\GAL4^4G/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Zhong and Wu, 2004)

Scer\GAL4^Mef2.247/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Schwaerzel et al., 2003)

Scer\GAL4^c115/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^Tab2-201Y/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^elav-C155/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Zars et al., 2000)

Fails to rescue

Scer\GAL4^5-HT1B.PY, Scer\GAL80^Mef2.mb247, Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Xie et al., 2013)

Scer\GAL4^c305a/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Xie et al., 2013)

Adcy1^UAS.cZa/Scer\GAL4^17d fails to rescue Adcy1^rut-1

(Scheunemann et al., 2012)

Adcy1^UAS.cZa/Scer\GAL4^NP1131 fails to rescue Adcy1^rut-1

(Scheunemann et al., 2012)

Adcy1^UAS.cZa/Scer\GAL4^c320 fails to rescue Adcy1^rut-1

(Scheunemann et al., 2012)

Scer\GAL4^c305a/Adcy1^UAS.cZa fails to rescue Adcy1^rut-1

(Scheunemann et al., 2012)

Adcy1^UAS.cZa/Scer\GAL4^GH146 fails to rescue Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^NP2320/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Liu et al., 2006)

Adcy1^UAS.cZa/Scer\GAL4^BG487 fails to rescue Adcy1^rut-2080

(Zhong and Wu, 2004)

Adcy1^UAS.cZa/Scer\GAL4^103Y fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^121Y/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^insc-Mz1407/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^238Y/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^30Y/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^dbr-72Y/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^Alp4-c232/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^Tab2-201Y/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^H24/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^c492b/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Adcy1^UAS.cZa/Scer\GAL4^lilli-189Y fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Scer\GAL4^Alp4-c232/Adcy1^UAS.cZa fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Adcy1^UAS.cZa/Scer\GAL4^lilli-189Y fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Adcy1^UAS.cZa/Scer\GAL4^17d fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Comments

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^ey-OK107 or Scer\GAL4^Mef2.247, but not Scer\GAL4^17d, Scer\GAL4^NP1131, Scer\GAL4^c320, or Scer\GAL4^c305a, rescues the defective short term memory performance of rut¹/rut¹ mutants.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Mef2.247, but not Scer\GAL4^17d or Scer\GAL4^NP1131, rescues the defective anaethesia-sensitive memory performance of rut¹/rut¹ mutants.

(Scheunemann et al., 2012)

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Tdc2.PC rescues the decrease in synaptopods seen at third instar larval neuromuscular junctions in rut²⁰⁸⁰ mutants.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Tdc2.PC rescues the response to starvation block seen in rut²⁰⁸⁰ mutants.

(Koon et al., 2011)

Expression of rut^Scer\UAS.cZa under the control of one of Scer\GAL4²⁴⁷, Scer\GAL4^c309 or Scer\GAL4^OK107 significantly improves the 2 minute memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^OK107 significantly improves the 3 hour memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^c309 significantly improves the memory performance of rut²⁰⁸⁰/Y males 24 hours after massed training.

Expression of rut^Scer\UAS.cZa under the control of either Scer\GAL4^c309 or Scer\GAL4^OK107 significantly improves the memory performance of rut²⁰⁸⁰/Y males 24 hours after spaced training.

Expression of rut^Scer\UAS.cZa under the control of either Scer\GAL4^GH146 or Scer\GAL4^c305a does not improve either 2 minute or 3 hour memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^GH146 does not improve the memory performance of rut²⁰⁸⁰/Y males 24 hours after either massed or spaced training.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Tab2-201Y significantly improves the 2 minute memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session, but does not improve the 3 hour memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Tab2-201Y significantly improves the memory performance of rut²⁰⁸⁰/Y males 24 hours after massed training, but does not significantly improve the memory performance of rut²⁰⁸⁰/Y males 24 hours after spaced training.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^c739 does not improve either 2 minute or 3 hour memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^c739 significantly improves the memory performance of rut²⁰⁸⁰/Y males 24 hours after massed or spaced training.

Expression of rut^Scer\UAS.cZa under the control of both Scer\GAL4^Tab2-201Y and Scer\GAL4^c739 simultaneously results in nearly normal 2 minute memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of both Scer\GAL4^Tab2-201Y and Scer\GAL4^c739 simultaneously significantly improves the 3 hour memory performance of rut²⁰⁸⁰/Y males after a single aversive Pavlovian training session.

Expression of rut^Scer\UAS.cZa under the control of both Scer\GAL4^Tab2-201Y and Scer\GAL4^c739 simultaneously significantly improves the memory performance of rut²⁰⁸⁰/Y males 24 hours after massed or spaced training.

Expression of rut^Scer\UAS.cZa under the control of both Scer\GAL4^Tab2-201Y and Scer\GAL4^c305a simultaneously does not significantly improve the memory performance of rut²⁰⁸⁰/Y males 24 hours after spaced training.

(Blum et al., 2009)

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf restores the increase in daytime sleep and daytime sleep-bout duration in rut²⁰⁸⁰ mutants.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^{elav.Switch.PO} restores the increase in daytime sleep and daytime sleep-bout duration in rut²⁰⁸⁰ mutants.

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Mz717 restores the increase in daytime sleep and daytime sleep-bout duration in rut²⁰⁸⁰ mutants.

(Donlea et al., 2009)

The loss of visual pattern memory (only the "elevation" parameter has been tested) seen in rut²⁰⁸⁰ flies is rescued by expression of rut^Scer\UAS.cZa under the control of one of Scer\GAL4^104Y, Scer\GAL4^121Y or Scer\GAL4^elav.PLu. Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^c205 rescues the visual pattern memory for the "elevation" parameter, but not for "size" or "contour orientation" in rut²⁰⁸⁰ flies. This rescue of the "elevation" parameter in rut²⁰⁸⁰ flies by rut^Scer\UAS.cZa is also seen in flies in which Scer\GAL80^ts.αTub84B expression is used to block Scer\GAL4^c205 function throughout development, but it is then inactivated by raising adult flies at 30^oC for 14 hours (allowing Scer\GAL4^c205 to drive expression of rut^Scer\UAS.cZa in the adults). Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^NP6510 or Scer\GAL4^NP6561 rescues the visual pattern memory for the "contour orientation" parameter, but not for "size" or "elevation" in rut²⁰⁸⁰ flies. This rescue of the "contour orientation" parameter in rut²⁰⁸⁰ flies by rut^Scer\UAS.cZa is also seen in flies in which Scer\GAL80^ts.αTub84B expression is used to block Scer\GAL4^NP6510 function throughout development, but it is then inactivated by raising adult flies at 30^oC for 14 hours (allowing Scer\GAL4^NP6510 to drive expression of rut^Scer\UAS.cZa in the adults). Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^NP2320 fails to rescue the visual pattern memory for the "elevation", "size" or "contour orientation" parameters in rut²⁰⁸⁰ flies.

(Liu et al., 2006)

Expression of rut^Scer\UAS.cZa is able to suppress the increased resistance to V. cholerae infection seen in rut²⁰⁸⁰ mutant flies in the absence of a Scer\GAL4 driver.

(Blow et al., 2005)

The short term memory deficit of rut²⁰⁸⁰ mutants is completely rescued by rut^Scer\UAS.cZa; Scer\GAL4^{Mef2.247.Switch} after feeding with 500μM RU486 for 2 days. Shorter RU486 treatments produced incomplete rescue. The short term memory deficit of rut^MB2769 mutants is completely rescued by rut^Scer\UAS.cZa; Scer\GAL4^{Mef2.247.Switch} after feeding with 500μM RU486 for 2 days.

(Mao et al., 2004)

Expression of rut^Scer\UAS.cZa in a sunset of nerves, under the control of Scer\GAL4^4G, increases levels of motor axon termini arborization in rut²⁰⁸⁰ larvae raied at 30^oC, but does not increase levels to those seen in wild-type larvae.

(Zhong and Wu, 2004)

Rescue of rut²⁰⁸⁰ by rut^Scer\UAS.cZa, Scer\GAL4^c747 can be repressed by Scer\GAL80^ts.αTub84B at the permissive temperture (19^oC) but not at the restrictive temperature (30^oC).

(McGuire et al., 2003)

Expression of rut^Scer\UAS.cZa under the control of Scer\GAL4^Mef2.247 rescues the limited olfactory learning phenotype of rut²⁰⁸⁰ mutants.

(Schwaerzel et al., 2003)

MB-rescued rut²⁰⁸⁰ mutant flies (through expression of rut^Scer\UAS.cZa by Scer\GAL4²⁴⁷) show extinction and extinction control performance indistinguishable from wild-type flies.

(Schwaerzel et al., 2002)

Rescue statements based on rescue of the learning and 3 minute memory defect of rut²⁰⁸⁰ flies. Rescue of rut²⁰⁸⁰ by Scer\GAL4^c522 depends on specific insertion of P{UAS-rut.Z}. Rescue is evident with P{UAS-rut.Z}1 but not P{UAS-rut.Z}2.

(Zars et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9406

P{lArB}Adcy1^MB2769; P{UAS-Adcy1.Z}1

9405

P{lArB}Adcy1^rut-2080; P{UAS-Adcy1.Z}2

Notes on Origin

Discoverer

Comments

Temperature shift experiments indicate that rut expression in the mushroom bodies of adult flies is necessary and sufficient to correct the olfactory memory defects in rut mutants - ruling out a need for rut expression during development of the mushroom bodies.

(McGuire et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Adcy1^UAS.cZa

rut^Scer\UAS.cMa

rut^Scer\UAS.cZa

rut^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zars

(Zars et al., 2000)

Secondary FlyBase IDs

FBal0154665

References (23)

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