FlyBase Allele Report: Dmel\rin[2]

General Information

Symbol

Dmel\rin²

Species

D. melanogaster

Name

FlyBase ID

FBal0117390

Feature type

allele

Associated gene

Dmel\rin

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Pazman et al., 2000)

Mutagen

P-element activity

(Pazman et al., 2000)

Progenitor genotype

P{lacW}rin¹

(Pazman et al., 2000)

rin¹

(Pazman et al., 2000)

Caused by aberration

Df(3R)rin²

Cytology

Description

Imprecise excision removing most of the P-element resulting in an approximately 14kb deletion which removes the entire rin open reading frame, the Rbp4 gene and part of the Hrb87F gene.

(Pazman et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:13,647,498..13,660,584 [+]

Comment:

Deficiency maps to 3R:9473220..9486306 , R5 coordinates.

(Baumgartner et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | recessive

(Pazman et al., 2000)

abnormal size | adult stage | somatic clone

(Baumgartner et al., 2013)

increased cell number | somatic clone

(Baumgartner et al., 2013)

viable

(Baumgartner et al., 2013, Pazman et al., 2000)

viable (with rin¹)

(Pazman et al., 2000)

viable (with rin^NP3248)

(Baumgartner et al., 2013)

viable (with rin^NP5420)

(Baumgartner et al., 2013)

visible (with rin¹)

(Pazman et al., 2000)

visible | recessive

(Pazman et al., 2000)

Phenotype Manifest In

egg | maternal effect (with rin³)

(Costa et al., 2013)

embryonic/larval cuticle | maternal effect (with rin³)

(Costa et al., 2013)

eye

(Pazman et al., 2000)

eye (with rin¹)

(Pazman et al., 2000)

eye disc

(Pazman et al., 2000)

eye photoreceptor cell

(Pazman et al., 2000)

ommatidium

(Pazman et al., 2000)

ommatidium (with rin¹)

(Pazman et al., 2000)

ommatidium | somatic clone | increased number

(Baumgartner et al., 2013)

Detailed Description

Statement

Reference

Homozygotes are viable and do not display obvious growth phenotypes.

rin²/rin^NP3248 and rin²/rin^NP5420 animals are viable without phenotypic alterations.

rin² mutant eyes, generated by eyFLP/FRT-mediated mitotic recombination, show an increase in ommatidial number, without variability, under normal food conditions.

(Baumgartner et al., 2013)

About 10% of eggs produced by rin³/rin² mothers are ventralised. While the remainder of the eggs show seemingly normal eggshells, cuticle preparations of the embryos produced by rin³/rin² mothers reveal severe defects in embryonic development. For example, many of the embryos are underdeveloped or have only scraps of cuticle.

(Costa et al., 2013)

Homozygotes and rin¹/rin² flies are viable and show a mild rough eye phenotype. Homozygous eyes show defects in both photoreceptor recruitment and ommatidial polarity. Many ommatidia have fewer than the wild-type complement of 8 photoreceptors and some have extra photoreceptors. Both inner and outer photoreceptors can be affected. Many mutant ommatidia with the normal complement of photoreceptors show polarity defects; both the degree and direction of rotation and the chirality of the ommatidia can be affected. The recruitment and polarity defects are evident in third larval instar imaginal discs.

(Pazman et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fmr1^Δ50M/Fmr1^Δ113M, rin^NP5420/rin² has short lived phenotype, suppressible by rin^mCherry

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has abnormal size | somatic clone | adult stage phenotype, suppressible by Fmr1^+t14

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has abnormal size | somatic clone | adult stage phenotype, suppressible by rin^+t11.8

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has abnormal size | somatic clone | adult stage phenotype, suppressible | partially by rin^mCherry

(Baumgartner et al., 2013)

Fmr1^Δ50M/Fmr1^Δ113M, rin^NP3248/rin² has lethal - all die during pupal stage phenotype, suppressible by rin^mCherry

(Baumgartner et al., 2013)

Enhancer of

Statement

Reference

rin²/rin[+] is an enhancer of abnormal planar polarity phenotype of Rho1^UAS.cMa, Scer\GAL4^hs.2sev

(Pazman et al., 2000)

Suppressor of

Statement

Reference

rin²/rin[+] is a suppressor of visible phenotype of Ras85D^V12.sev

(Pazman et al., 2000)

Other

Statement

Reference

Fmr1^Δ113M, rin² has partially lethal - majority die phenotype

(Baumgartner et al., 2013)

Capr^KK100145, Scer\GAL4^Act5C.PI, rin² has abnormal size | somatic clone phenotype

(Baumgartner et al., 2013)

Capr^KK100145, Fmr1^Δ113M, Scer\GAL4^Act5C.PI, rin² has lethal - all die during pupal stage phenotype

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has abnormal size | somatic clone | adult stage | nutrition conditional phenotype

(Baumgartner et al., 2013)

BacA\p35^UAS.cHa, Fmr1^Δ113M, Scer\GAL4^Act5C.PI, rin² has majority die during pharate adult stage phenotype

(Baumgartner et al., 2013)

Fmr1^Δ50M/Fmr1^Δ113M, rin^NP5420/rin² has abnormal size | pupal stage phenotype

(Baumgartner et al., 2013)

Scer\GAL4^Act5C.PI, lig^RNAi.UAS.II, rin² has abnormal size | somatic clone phenotype

(Baumgartner et al., 2013)

Fmr1^Δ50M/Fmr1^Δ113M, rin^NP3248/rin² has lethal - all die during pupal stage phenotype

(Baumgartner et al., 2013)

Fmr1^Δ50M/Fmr1^Δ113M, rin^NP5420/rin² has short lived phenotype

(Baumgartner et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fmr1^Δ113M, rin² has ommatidium | increased number | somatic clone phenotype, suppressible by Fmr1^+t14

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has ommatidium | increased number | somatic clone phenotype, suppressible by rin^+t11.8

(Baumgartner et al., 2013)

Fmr1^Δ113M, rin² has ommatidium | increased number | somatic clone phenotype, suppressible | partially by rin^mCherry

(Baumgartner et al., 2013)

Enhancer of

Statement

Reference

rin²/rin[+] is an enhancer of ommatidium phenotype of Rho1^UAS.cMa, Scer\GAL4^hs.2sev

(Pazman et al., 2000)

rin²/rin[+] is an enhancer of photoreceptor neuron phenotype of Rho1^UAS.cMa, Scer\GAL4^hs.2sev

(Pazman et al., 2000)

rin²/rin[+] is an enhancer of photoreceptor cell R7 phenotype of Sos^JC2, sev⁶

(Pazman et al., 2000)

NOT Enhancer of

Statement

Reference

rin² is a non-enhancer of phenotype of RasGAP1^UAS.cGa, Scer\GAL4^hs.2sev

(Pazman et al., 2000)

Suppressor of

Statement

Reference

rin²/rin[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Pazman et al., 2000)

rin²/rin[+] is a suppressor of phenotype of sev^S11.Tag:MYC

(Pazman et al., 2000)

rin²/rin[+] is a suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Pazman et al., 2000)

rin²/rin[+] is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Pazman et al., 2000)

NOT Suppressor of

Statement

Reference

rin² is a non-suppressor of phenotype of RasGAP1^UAS.cGa, Scer\GAL4^hs.2sev

(Pazman et al., 2000)

rin² is a non-suppressor of phenotype of Raf::tor^12D.sev

(Pazman et al., 2000)

rin² is a non-suppressor of phenotype of rl^Sem

(Pazman et al., 2000)

Other

Statement

Reference

Fmr1^Δ113M, rin² has ommatidium | increased number | somatic clone | nutrition conditional phenotype

(Baumgartner et al., 2013)

Capr^KK100145, Scer\GAL4^Act5C.PI, rin² has ommatidium | somatic clone phenotype

(Baumgartner et al., 2013)

Capr^KK100145, Fmr1^Δ113M, Scer\GAL4^Act5C.PI, rin² has eye | somatic clone | pupal stage phenotype

(Baumgartner et al., 2013)

BacA\p35^UAS.cHa, Fmr1^Δ113M, Scer\GAL4^Act5C.PI, rin² has eye | somatic clone phenotype

(Baumgartner et al., 2013)

Scer\GAL4^Act5C.PI, lig^RNAi.UAS.II, rin² has ommatidium | somatic clone phenotype

(Baumgartner et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Most rin² Fmr1^Δ113M double homozygous larvae die at an early stage, but a few escapers that reach the early pupal stage form long, slender pupae.

rin^NP3248/rin², Fmr1^Δ113M/Fmr1^Δ50M animals reach a late pupal stage - pupae are long and slender.

rin^NP5420/rin², Fmr1^Δ113M/Fmr1^Δ50M animals develop into adult flies that die soon after eclosion - pupae are long and slender.

rin^{T:Disc\RFP-mCherry} rescues the slender pupae phenotype and lethality associated with rin^NP3248/rin², Fmr1^Δ113M/Fmr1^Δ50M animals.

rin^{T:Disc\RFP-mCherry} rescues the slender pupae phenotype and lethality associated with rin^NP5420/rin², Fmr1^Δ113M/Fmr1^Δ50M animals.

rin² Fmr1^Δ113M double mutant eyes, generated by eyFLP/FRT-mediated mitotic recombination, show an increase in ommatidial number under normal food conditions. (Double mutants raised on food with reduced yeast content display eyes with a constant increase in ommatidial number, while mutants raised on increased yeast content show more pronounced variability in ommatidial number.) This phenotype is rescued to a rin² like phenotype by inclusion of Fmr1^+t14, and to a Fmr1^Δ113M-like phenotype by inclusion of rin^+t11.8, while rin^{T:Disc\RFP-mCherry} does not completely rescue to a Fmr1^Δ113M-like phenotype.

Eyes overexpressing Capr^KK100145 in a rin² background, generated using the eyFLP Actin-Flp out-Gal4 system, are overgrown owing to more ommatidia compared to controls.

Animals with eyes overexpressing Capr^KK100145 in a Fmr1^Δ113M rin² background, generated using the eyFLP Actin-Flp out-Gal4 system, results in late pupal lethality, with strongly overgrown eye/head structures visible in the pharates.

Eyes expressing lig^{dsRNA.Scer\UAS.II} in a rin² background, generated using the eyFLP Actin-Flp out-Gal4 system, are overgrown owing to more ommatidia compared to controls.

(Baumgartner et al., 2013)

The extra R7 photoreceptor cells seen in flies carrying sev^{S11.T:Hsap\MYC} are reduced to an average of 2.32 +/- 0.11 R7 photoreceptor cells per ommatidium if the flies are also carrying rin². 18.7% of ommatidia have an R7 cell in sev⁶; Sos^JC2 flies. This partial rescue of sev⁶ by Sos^JC2 is relieved by rin²; 18.2% of ommatidia have an R7 cell in sev⁶; Sos^JC2/+ ; rin²/+ flies.

28.7% of ommatidia have an R7 cell in sev⁶ ; Sos^JC2 flies. This partial rescue of sev⁶ by Sos^JC2 is relieved by rin¹ or rin²; 13.9% and 18.2% of ommatidia have an R7 cell in sev⁶ ; Sos^JC2/+ ; rin¹/+ and sev⁶ ; Sos^JC2/+ ; rin²/+ flies respectively.

(Pazman et al., 2000)

Xenogenetic Interactions

Statement

Reference

Animals with rin² Fmr1^Δ113M double mutant eyes over-expressing BacA\p35^Scer\UAS.cHa via eyFLP, Actin-Flp out-Gal4/FRT-mediated mitotic recombination, die as pharate adults except a few escapers that display massively overgrown eye structures.

(Baumgartner et al., 2013)

Complementation and Rescue Data

Rescued by

rin¹/rin² is rescued by rin^Tub.PP

(Pazman et al., 2000)

rin² is rescued by rin^Tub.PP

(Pazman et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)