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General Information
Symbol
Dmel\par-1W3
Species
D. melanogaster
Name
FlyBase ID
FBal0117403
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Excision of the P{EP} element. There is a 12.5kb deletion removing all of the coding sequence for the par-1 kinase domain as well as most of the linker region. In addition, there is a small deletion adjacent to the remaining P-element sequence in the first intron (this small deletion is within both the mei-W68 and par-1 transcription units as mei-W68 shares a promoter and 5' UTR with the N1 class of par-1 transcripts).

Insertion components
P{?EP}mei-W68W3
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

par-1W3 mutant clones are significantly smaller than the corresponding wild type controls.

Germline stem cells (GSCs) in newly eclosed par-1k06323/par-1W3 males show a high frequency of centrosome misorientation and frequently undergo mitosis with misoriented spindles (mitosis with misoriented spindles is not seen in wild-type male GSCs).

Border follicle cell migration is disrupted in mosaic egg chambers containing homozygous follicle cell clones.

Border follicle cell migration is disrupted in par-1k06323/par-1W3 females.

par-1W3 homozygous mutant eye disc clones display normal cell polarity. However, the eye disc epithelium appears to locally deform basally in regions containing a par-1W3 clone.

Mutant follicle cells show an increase in the density of microtubules compared to their wild-type neighbours in mosaic egg chambers (when the egg chambers are fixed using an optimised procedure for preserving microtubules).

par-1k06323/par-1W3 animals show loss of oocyte polarity. The progeny of par-1k06821b/par-1W3 females show loss of abdominal segments.

par-1W3/par-1k06323 oocytes show misorganisation of the microtubule network.

Egg chambers containing homozygous germline clones do develop, but they remain small and never reach the stage where the oocyte is larger than the nurse cells. The mutant cysts develop 16 nurse cells and no oocyte; none of the cells in the mutant egg chambers forms a karyosome (this is normally formed in the oocyte) and instead all 16 germ cells become polyploid. In contrast to wild type, mutant cysts contain up to 16 cells with synaptonemal complex (SC) in early region 2a, indicating that all of the cells have entered meiosis. The SC becomes progressively restricted to two cells and then to one cell in region 3. This restriction is delayed compared to wild-type cysts, where the SC is always restricted to the oocyte by region 2b. In addition, the SC disappears prematurely from mutant oocytes, being undetectable in stage 2 egg chambers (wild-type oocytes retain a compacted SC until stage 3-4). The centrosomes accumulate at the anterior of the oocyte in late region 2b or early region 3, indicating that the first phase of centrosome migration occurs normally. They remain at the anterior of the oocyte however, and never translocate to the posterior (as occurs in wild type). There is a slight delay in the focusing of the microtubules to one cell in region 2b, however, the overall organisation of microtubules in region 2 appears essentially normal. The formation and asymmetric segregation of the fusome appears normal.

Germline clones of par-1W3 cause oogenesis to arrest at stage 5. The progeny of par-1W3/par-1k06323 females show typical posterior group phenotypes; the embryos lack abdominal segments (only 5% of embryos and hatched larvae have the wild-type abdominal pattern and the average number of denticle belts is 0.7) and pole cells fail to form, giving rise to a grandchildless phenotype in which adult escapers have agametic gonads. Migration of the nucleus to the anterior occurs normally in par-1W3/par-1k06323 oocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

par-1W3/par-1[+] is a suppressor | partially of neuroanatomy defective | adult stage phenotype of TaoEP1455

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

par-1W3/par-1[+] is an enhancer of embryonic/larval cuticle phenotype of baz4

par-1W3/par-1[+] is an enhancer of embryonic/larval cuticle phenotype of bazG0484

par-1W3/par-1[+] is an enhancer of oocyte phenotype of 14-3-3εj2B10

NOT Enhancer of
Suppressor of
Statement
Reference

par-1W3/par-1[+] is a suppressor | partially of mushroom body alpha-lobe phenotype of TaoEP1455

par-1W3/par-1[+] is a suppressor | partially of mushroom body beta-lobe phenotype of TaoEP1455

par-1W3/par-1[+] is a suppressor | partially of mushroom body gamma-lobe phenotype of TaoEP1455

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

par-1W3/+ rescues the abnormal centrosome positioning of embryos from mothers expressing aPKCHMS01320 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

One copy of par-1W3 partially suppresses the adult mushroom body morphology defects seen in TaoEP1455. Approximately half of all brain hemispheres have fully formed mushroom body lobes.

One copy of par-1W3 partially suppresses the adult mushroom body morphology defects seen when TaoGD8262 is weakly expressed in mushroom bodies under the control of Scer\GAL4ey-OK107.

The cuticle phenotype of dead embryos derived from baz4/+ embryos derived from a cross of baz4/+ females to wild-type males is enhanced if the females also carry one copy of par-1W3.

The cuticle phenotype of dead embryos derived from bazG0484/+ embryos derived from a cross of bazG0484/+ females to wild-type males is enhanced if the females also carry one copy of par-1W3.

The maternal effect embryonic phenotype of par-1k06821b/par-1W3 is enhanced by heterozygosity for lkb14B1-11 or lkb14A4-2. The loss of anterior-posterior polarity seen in the oocytes of par-1k06323/par-1W3 animals is partially rescued by lkb1Scer\UAS.P\T.T:Avic\GFP; Scer\GAL4mat.αTub67C.T:Hsim\VP16, but is not rescued by lkb1K147M.Scer\UAS.P\T.T:Avic\GFP; Scer\GAL4mat.αTub67C.T:Hsim\VP16.

In 14-3-3εj2B10 mutant egg chambers that develop an oocyte, the penetrance of oocyte polarization defects in oocytes at stage 10 is dominantly enhanced by par-1W3.

Xenogenetic Interactions
Statement
Reference

The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified by par-1W3.

Complementation and Rescue Data
Partially rescued by
Comments

Expression of par-1N1L.αTub67C.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 fully rescues the posterior localisation of osk mRNA.

Expression of par-1N2S.αTub67C.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 partially rescues (approximately 30% mislocalisation) the posterior localisation of osk mRNA.

Expression of par-1N3L.αTub67C.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 partially rescues (approximately 40% mislocalisation) the posterior localisation of osk mRNA.

Expression of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 fully rescues the posterior localisation of osk mRNA.

par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 rescues the posterior movement of the centrosomes and the maintenance of oocyte identity when expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in par-1W3 germline clones.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (35)