Allele Dmel\pAbp^EP310

General Information
Symbol	Dmel\pAbp^EP310	Species	D. melanogaster
Name		FlyBase ID	FBal0117409
Feature type	allele	Associated gene	Dmel\pAbp
Also Known As	EP310

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Sigrist et al., 2000, Gene Disruption Project members, 2001-)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:14,033,247..14,033,247 (Sigrist et al., 2000, Reiff et al., 2002, Gene Disruption Project members, 2001-, Hekmat-Scafe et al., 2005, Blagden et al., 2009, Cziko et al., 2009, Pertceva et al., 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion into the first 3' non-coding exon. (Blagden et al., 2009) P{EP} insertion in the 3' untranslated region. (Hekmat-Scafe et al., 2005) P{EP} insertion into the first 3' non-coding exon. (Sigrist et al., 2000)
Caused by insertion	P{EP}pAbp^EP310 (Sigrist et al., 2000, Reiff et al., 2002, Gene Disruption Project members, 2001-, Hekmat-Scafe et al., 2005, Blagden et al., 2009, Cziko et al., 2009, Pertceva et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	male sterile (Blagden et al., 2009) male sterile (with pAbp^k10109) (Blagden et al., 2009) meiotic cell cycle defective (Blagden et al., 2009) meiotic cell cycle defective (with pAbp^k10109) (Blagden et al., 2009) neuroanatomy defective (with Df(2R)Pcl7B), with Scer\GAL4[-]: (Sigrist et al., 2000) neurophysiology defective (with Df(2R)Pcl7B), with Scer\GAL4[-]: (Sigrist et al., 2000) viable (Pertceva et al., 2010)
Phenotype Manifest In
	bouton (with Df(2R)Pcl7B), with Scer\GAL4[-]: (Sigrist et al., 2000) class IV dendritic arborizing neuron \| somatic clone, with Scer\GAL4^Act.PU (Cziko et al., 2009) neuromuscular junction (with Df(2R)Pcl7B), with Scer\GAL4[-]: (Sigrist et al., 2000) spindle & spermatocyte (Blagden et al., 2009) spindle & spermatocyte (with pAbp^k10109) (Blagden et al., 2009)
Detailed Description
	Statement Reference 17% of homozygous and 18% of pAbp[EP310]/pAbp[k10109] spermatocytes show defects in meiosis, with the most common phenotype being detachment of the meiotic spindles from astral microtubules. (Blagden et al., 2009) Over-expression of pAbp[EP310] reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4[Act.PU]). Total dendrite length is significantly reduced in these neurons. The rough eye phenotype observed in Fmr1[sev.PW] flies is suppressed in a pAbp[EP310]/+ background. (Cziko et al., 2009) The density of T-bars in the boutons of pAbp^EP310/Df(2R)Pcl7B mutants is the same as wild type. (Reiff et al., 2002) The mean amplitude of miniature postsynaptic currents (mEJCs) at the neuromuscular junction is indistinguishable from wild type in pAbp^EP310/Df(2R)Pcl7B flies. Evoked postsynaptic currents (eEJCs) are significantly larger than in wild-type. The number of junctional boutons per neuromuscular junction is increased compared to wild type. (Sigrist et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference pAbp^EP310 has meiotic cell cycle defective phenotype, enhanceable by larp^mtr-null/larp[+] (Blagden et al., 2009) pAbp^EP310 has meiotic cell cycle defective phenotype, enhanceable by larp^S003108/larp[+] (Blagden et al., 2009)
Enhancer of
Statement Reference pAbp[+]/pAbp^EP310 is an enhancer of meiotic cell cycle defective phenotype of larp^S003108 (Blagden et al., 2009) pAbp[+]/pAbp^EP310 is an enhancer of visible phenotype of os¹ (Mukherjee et al., 2006)
NOT Enhancer of
Statement Reference pAbp[+]/pAbp^EP310 is a non-enhancer of visible phenotype of os^GMR.PB (Mukherjee et al., 2006)
Suppressor of
Statement Reference Scer\GAL4^elav.PLu/pAbp^EP310 is a suppressor \| partially of bang sensitive phenotype of eas² (Hekmat-Scafe et al., 2005)
NOT Suppressor of
Statement Reference pAbp[+]/pAbp^EP310 is a non-suppressor of visible phenotype of os^GMR.PB (Mukherjee et al., 2006)
Other
Statement Reference larp^mtr-null/larp[+], pAbp^EP310 has meiotic cell cycle defective \| dominant phenotype (Blagden et al., 2009) larp^S003108/larp[+], pAbp^EP310 has meiotic cell cycle defective \| dominant phenotype (Blagden et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference pAbp^EP310 has spermatocyte phenotype, enhanceable by larp^mtr-null/larp[+] (Blagden et al., 2009) pAbp^EP310 has spermatocyte phenotype, enhanceable by larp^S003108/larp[+] (Blagden et al., 2009)
Enhancer of
Statement Reference pAbp[+]/pAbp^EP310 is an enhancer of eye phenotype of os¹ (Mukherjee et al., 2006) pAbp[+]/pAbp^EP310 is an enhancer of spermatocyte phenotype of larp^S003108 (Blagden et al., 2009)
NOT Enhancer of
Statement Reference pAbp[+]/pAbp^EP310 is a non-enhancer of eye phenotype of os^GMR.PB (Mukherjee et al., 2006)
Suppressor of
Statement Reference pAbp[+]/pAbp^EP310 is a suppressor of eye phenotype of Fmr1^sev.PW (Cziko et al., 2009)
NOT Suppressor of
Statement Reference pAbp[+]/pAbp^EP310 is a non-suppressor of eye phenotype of os^GMR.PB (Mukherjee et al., 2006)
Other
Statement Reference larp^mtr-null/larp[+], pAbp^EP310 has spermatocyte phenotype (Blagden et al., 2009) larp^S003108/larp[+], pAbp^EP310 has spermatocyte phenotype (Blagden et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference 23% of pAbp[EP310]/+ ; larp[mtr-null]/+ double heterozygous spermatocytes show defects in meiosis. 16% of pAbp[EP310]/+ ; larp[S003108]/+ double heterozygous spermatocytes show defects in meiosis. The frequency of homozygous pAbp[EP310] spermatocytes that show defects in meiosis (17%) is increased by larp[mtr-null]/+ (to 31.4%) or larp[S003108]/+ (to 29.3%). (Blagden et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	17261 w¹¹¹⁸; P{EP}pAbp^EP310
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	EP310 (Sigrist et al., 2000, Mukherjee et al., 2006) pAbp^EP0310 pAbp^EP310 (Blagden et al., 2009, Pertceva et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Pertceva et al., 2010, Cell Biol. Int. 34(10): 991--996 The role of Drosophila hyperplastic discs gene in spermatogenesis. [FBrf0211767] Blagden et al., 2009, Dev. Biol. 334(1): 186--197 Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. [FBrf0208825] Cziko et al., 2009, Genetics 182(4): 1051--1060 Genetic Modifiers of dFMR1 Encode RNA Granule Components in Drosophila. [FBrf0208636] Mukherjee et al., 2006, Genetics 172(3): 1683--1697 Identification of Drosophila genes modulating janus kinase/signal transducer and activator of transcription signal transduction. [FBrf0190752] Hekmat-Scafe et al., 2005, Genetics 169(3): 1477--1493 Seizure suppression by gain-of-function escargot mutations. [FBrf0183998] Reiff et al., 2002, J. Neurosci. 22(21): 9399--9409 Differential regulation of active zone density during long-term strengthening of Drosophila neuromuscular junctions. [FBrf0152148] Sigrist et al., 2000, Nature 405(6790): 1062--1065 Postsynaptic translation affects the efficacy and morphology of neuromuscular junctions. [FBrf0128816]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\pAbpEP310

Allele Dmel\pAbp^EP310