FlyBase Allele Report: Dmel\p53[H159N.UAS.Ex]

General Information

Symbol

Dmel\p53^H159N.UAS.Ex

Species

D. melanogaster

Name

FlyBase ID

FBal0117416

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{UAS-p53.H159N.Ex}

Also Known As

p53^DN, UAS-Dp53^H159N, UAS-p53^H159N

Key Links

Transgenic product class

missense_variant

(Ollmann et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ollmann et al., 2000)

Mutagen

in vitro construct

(Ollmann et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-p53.H159N.Ex}

Cytology

Description

Expression of p53 open reading frame is driven by the UAS regulatory sequences. The alpha tubulin 3' UTR provides stability to the message. Amino acid replacement: H159N.

(Ollmann et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

DOES NOT model cancer

CEA with pnut^GD1512

(Gerlach et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response, with Scer\GAL4^Cg.PU

(Barrio et al., 2014)

abnormal starvation stress response, with Scer\GAL4^ppl.PP

(Barrio et al., 2014)

abnormal starvation stress response, with Scer\GAL4^yolk

(Barrio et al., 2014)

Phenotype Manifest In

wing disc posterior compartment, with Scer\GAL4^en-e16E

(Ollmann et al., 2000)

Detailed Description

Statement

Reference

Expression of p53^{H159N.Scer\UAS.Ex} under the control of any of three fat body drivers (Scer\GAL4^ppl.PP, Scer\GAL4^cg.PU or Scer\GAL4^yolk) results in reduced survival rates upon adult nutrient deprivation when compared to controls. Expressing p53^{H159N.Scer\UAS.Ex} in muscles under the control of Scer\GAL4^Mef2.PR has no effect on survival rates upon fasting.

Fat-body specific expression of p53^{H159N.Scer\UAS.Ex} under the control of Scer\GAL4^ppl.PP causes an accelerated consumption of energy resources following fasting; triacylglycerides (TAGs) and glycogen are consumed at an accelerated rate compared to controls and the levels of total sugar are also reduced. Levels of circulating sugars are unaffected. The levels of TAGS, glycogen and sugars are largely similar in p53-depleted and control animals prior to starvation.

(Barrio et al., 2014)

Expression of p53^{H159N.Scer\UAS.Ex} under the control of Scer\GAL4^insc-Mz1407 has no effect on neuroblast number.

(Ouyang et al., 2011)

The expression of p53^{H159N.Scer\UAS.Ex} under the control of Scer\GAL4^en-e16E has little effect on viability.

(Schauer et al., 2009)

When expression is driven in the posterior compartment of the wing disc by Scer\GAL4^en-e16E, X irradiation-induced apoptosis is blocked. Wild type discs show cell cycle block upon X-irradiation - this is unaffected by p53^{H159N.Scer\UAS.Ex}.

(Ollmann et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

p53^H159N.UAS.Ex, Scer\GAL4^nub.PU is an enhancer of visible | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^nub.PU is an enhancer of increased cell death | third instar larval stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^vg.PU is an enhancer of visible phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU

(Schauer et al., 2009)

p53^H159N.UAS.Ex, Scer\GAL4^en-e16E is an enhancer of lethal phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E

(Schauer et al., 2009)

NOT Enhancer of

Statement

Reference

p53^H159N.UAS.Ex, Scer\GAL4^ey.PH is a non-enhancer of abnormal size | adult stage phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

p53^H159N.UAS.Ex, Scer\GAL4^ey.PH is a non-enhancer of visible phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Suppressor of

Statement

Reference

p53^H159N.UAS.Ex/Scer\GAL4^hh.PU is a suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

p53^H159N.UAS.Ex, Scer\GAL4^ppl.PP is a suppressor of abnormal starvation stress response phenotype of Dcr-1^GD11429, Scer\GAL4^ppl.PP

(Barrio et al., 2014)

NOT Suppressor of

Statement

Reference

p53^H159N.UAS.Ex/Scer\GAL4^ap-md544 is a non-suppressor of increased cell death | third instar larval stage phenotype of Scer\GAL4^ap-md544, pnut^GD1512

(Gerlach et al., 2018)

Scer\GAL4^109(2)80/p53^H159N.UAS.Ex is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Other

Statement

Reference

Fcp1^c.UAS, Scer\GAL4^vg.PU, p53^H159N.UAS.Ex has visible phenotype

(Schauer et al., 2009)

Fcp1^c.UAS, Scer\GAL4^en-e16E, p53^H159N.UAS.Ex has lethal phenotype

(Schauer et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

p53^H159N.UAS.Ex, Scer\GAL4^nub.PU is an enhancer of wing disc | third instar larval stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^vg.PU is an enhancer of wing phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU

(Schauer et al., 2009)

NOT Enhancer of

Statement

Reference

p53^H159N.UAS.Ex, Scer\GAL4^ey.PH is a non-enhancer of eye phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

Suppressor of

Statement

Reference

p53^H159N.UAS.Ex/Scer\GAL4^hh.PU is a suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

NOT Suppressor of

Statement

Reference

p53^H159N.UAS.Ex/Scer\GAL4^ap-md544 is a non-suppressor of wing disc dorsal compartment | third instar larval stage phenotype of Scer\GAL4^ap-md544, pnut^GD1512

(Gerlach et al., 2018)

p53^H159N.UAS.Ex, Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Scer\GAL4^109(2)80/p53^H159N.UAS.Ex is a non-suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80/p53^H159N.UAS.Ex is a non-suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

p53^H159N.UAS.Ex, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Scer\GAL4^insc-Mz1407/p53^H159N.UAS.Ex is a non-suppressor of neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Other

Statement

Reference

Fcp1^c.UAS, Scer\GAL4^vg.PU, p53^H159N.UAS.Ex has wing phenotype

(Schauer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of p53^H159N.UAS.Ex does not suppress the apoptosis in the third instar larval wing disc dorsal compartment induced by the expression of pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts]; this co-expression does not lead to wing disc tumors.

(Gerlach et al., 2018)

The extensive cell death in the pouch region of wing discs in third instar larvae as well as the small and deformed wings in adults expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU are further worsened by co-expression of p53^{H159N.Scer\UAS.Ex}.

(Bayona-Feliu et al., 2017)

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 is not significantly affected by co-expression of p53^{H159N.Scer\UAS.Ex}.

(Tsuyama et al., 2017)

Expression of p53^{H159N.Scer\UAS.Ex} suppresses the increase in survival rate in response to starvation in adult flies expressing Dcr-1^GD11429 in the fat body under the control of Scer\GAL4^ppl.PP.

(Barrio et al., 2014)

Co-expression of p53^{H159N.Scer\UAS.Ex} (that lacks transactivation activity) does not suppress supernumerary neuroblast formation induced by numb^{TS4D.Scer\UAS} expression under the control of Scer\GAL4^insc-Mz1407.

(Ouyang et al., 2011)

The lethality of animals expressing Fcp1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^en-e16E is shifted to an earlier phase if the animals are also co-expressing p53^{H159N.Scer\UAS.Ex}.

Co-expression of p53^{H159N.Scer\UAS.Ex} and Fcp1^Scer\UAS.cTa under the control of Scer\GAL4^vg.PU results in a nicked wings phenotype (this phenotype is not seen when either transgene is expressed alone).

(Schauer et al., 2009)

Xenogenetic Interactions

Statement

Reference

The additional co-expression of p53^{H159N.Scer\UAS.Ex} does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6^{Scer\UAS.T:Hsap\MYC} and Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PU.

(Padash Barmchi et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

8420

y¹ w¹¹¹⁸; P{UAS-p53.H159N.Ex}2

8421

y¹ w¹¹¹⁸; P{UAS-p53.H159N.Ex}3

8422

P{UAS-p53.H159N.Ex}1, y¹ w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dmp53H159N

(Merino et al., 2002)

p53^DN

(Ewen-Campen and Perrimon, 2024, Li et al., 2020)

p53^{H159N.Scer\UAS.Ex}

p53^H159N.UAS.Ex

p53[DN]

(Tsuyama et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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