FlyBase Allele Report: Dmel\p53[GMR.Ex]

General Information

Symbol

Dmel\p53^GMR.Ex

Species

D. melanogaster

Name

FlyBase ID

FBal0117418

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{GMR-p53.Ex}

Also Known As

GMR-p53, GMR-Dmp53, GMR-Dp53

Key Links

Transgenic product class

wild_type

(Ollmann et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ollmann et al., 2000)

Mutagen

in vitro construct

(Ollmann et al., 2000)

Progenitor genotype

Carried in construct

P{GMR-p53.Ex}

Cytology

Description

Construct: Expression of p53 open reading frame is driven by the GMR regulatory sequences. An alpha tubulin 3' UTR provides stability to the message.

(Ollmann et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates viral infectious disease

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

p53^GMR.Ex induces a small eye.

(Nagel et al., 2021)

Flies expressing p53^GMR.Ex have a reduced eye, due to increased cell death.

(Chakraborty et al., 2015)

Expression of p53^GMR.Ex produces flies with small and rough eyes with a glossy appearance. Massive cell death is seen in the posterior half of the third instar larval eye disc and the numbers of R7 photoreceptor neurons and cone cells are strongly reduced compared with wild type. All other photoreceptor neurons appear largely normal. In pupal p53^GMR.Ex eye disc ommatidia are severely misorganized, as indicated by enlarged interommatidial space, ommatidial fusions and reduced number of cone cells in each ommatidium.

(Fan et al., 2010)

Expression of p53^GMR.Ex in the posterior portion of late larval eye discs is sufficient to induce apoptosis.

(Colombani et al., 2006)

Expression of p53^GMR.Ex induces apoptosis in the developing eye disc, which results in a dramatic reduction in adult eye size compared to wild type.

(Kondo et al., 2006)

Flies expressing p53^GMR.Ex have eye tissue with no evidence of intact ommatidia or bristles.

(Price et al., 2002)

Adults are viable with small, rough eyes with fused ommatidia. This is due to widespread apoptosis in cells expressing p53^GMR.Ex. Cells initiate apoptosis within 3-6 hours after p53^GMR.Ex is expressed. p53^GMR.Ex neither blocks nor delays the transition from G1 to S phase in the second mitotic wave in the eye disc. However the pattern of mitosis is affected with entry into M phase or duration of M phase affected.

(Ollmann et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

p53^GMR.Ex has visible phenotype, enhanceable by Corp^95B

(Chakraborty et al., 2015)

p53^GMR.Ex has increased cell death phenotype, enhanceable by Corp^95B

(Chakraborty et al., 2015)

Suppressed by

Statement

Reference

p53^GMR.Ex has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Mnr^EP555

(Nagel et al., 2021)

p53^GMR.Ex has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Mnr^EP555

(Nagel et al., 2021)

p53^GMR.Ex has visible phenotype, suppressible by Scer\GAL4^GMR.PF/Corp^EY03495

(Chakraborty et al., 2015)

p53^GMR.Ex has increased cell death phenotype, suppressible by Scer\GAL4^GMR.PF/Corp^EY03495

(Chakraborty et al., 2015)

p53^GMR.Ex has increased cell death | third instar larval stage phenotype, suppressible | somatic clone by Dark^G8

(Fan et al., 2010)

p53^GMR.Ex has increased cell death | third instar larval stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has decreased cell number | third instar larval stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has abnormal size | adult stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has visible | adult stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has increased cell death phenotype, suppressible | partially by hpo^42-47

(Colombani et al., 2006)

p53^GMR.Ex has increased cell death phenotype, suppressible | partially by wts^x1

(Colombani et al., 2006)

p53^GMR.Ex has increased cell death phenotype, suppressible | partially by sav³

(Colombani et al., 2006)

p53^GMR.Ex has increased cell death | third instar larval stage phenotype, suppressible | somatic clone by Dronc^ΔA8/Dronc^ΔA8

(Kondo et al., 2006)

p53^GMR.Ex has visible phenotype, suppressible | partially by Hr4^EP1232/Scer\GAL4^GMR.PF

(Müller et al., 2005)

p53^GMR.Ex has visible phenotype, suppressible | partially by mir-278^EP2316/Scer\GAL4^GMR.PF

(Müller et al., 2005)

p53^GMR.Ex has visible phenotype, suppressible | partially by Scer\GAL4^GMR.PF/Imp^EP1433

(Müller et al., 2005)

p53^GMR.Ex has visible phenotype, suppressible by Myt1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has visible phenotype, suppressible by Wee1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has abnormal cell death phenotype, suppressible by Hsap\CDKN1A^GMR.Ex

(Ollmann et al., 2000)

p53^GMR.Ex has visible phenotype, suppressible by Hsap\CDKN1A^GMR.Ex

(Ollmann et al., 2000)

NOT suppressed by

Statement

Reference

p53^GMR.Ex has visible phenotype, non-suppressible | somatic clone by Dronc^ΔA8/Dronc^ΔA8

(Kondo et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

p53^GMR.Ex has eye phenotype, enhanceable by Corp^95B

(Chakraborty et al., 2015)

Suppressed by

Statement

Reference

p53^GMR.Ex has eye phenotype, suppressible | partially by Mnr^EP555/Scer\GAL4^GMR.PU

(Nagel et al., 2021)

p53^GMR.Ex has eye phenotype, suppressible by Scer\GAL4^GMR.PF/Corp^EY03495

(Chakraborty et al., 2015)

p53^GMR.Ex has eye disc | posterior | third instar larval stage phenotype, suppressible | somatic clone by Dark^G8

(Fan et al., 2010)

p53^GMR.Ex has eye disc | posterior | third instar larval stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has photoreceptor cell R7 | third instar larval stage phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has eye phenotype, suppressible by Hsap\CDKN1A^GMR.PH

(Fan et al., 2010)

p53^GMR.Ex has eye disc phenotype, suppressible | somatic clone by Dronc^ΔA8/Dronc^ΔA8

(Kondo et al., 2006)

p53^GMR.Ex has eye phenotype, suppressible | partially by Hr4^EP1232/Scer\GAL4^GMR.PF

(Müller et al., 2005)

p53^GMR.Ex has eye phenotype, suppressible | partially by mir-278^EP2316/Scer\GAL4^GMR.PF

(Müller et al., 2005)

p53^GMR.Ex has eye phenotype, suppressible | partially by Scer\GAL4^GMR.PF/Imp^EP1433

(Müller et al., 2005)

p53^GMR.Ex has eye phenotype, suppressible by Myt1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has eye phenotype, suppressible by Wee1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has interommatidial bristle phenotype, suppressible by Myt1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has interommatidial bristle phenotype, suppressible by Wee1^GMR.PP

(Price et al., 2002)

p53^GMR.Ex has eye disc phenotype, suppressible by Hsap\CDKN1A^GMR.Ex

(Ollmann et al., 2000)

p53^GMR.Ex has eye phenotype, suppressible by Hsap\CDKN1A^GMR.Ex

(Ollmann et al., 2000)

NOT suppressed by

Statement

Reference

p53^GMR.Ex has eye phenotype, non-suppressible | somatic clone by Dronc^ΔA8/Dronc^ΔA8

(Kondo et al., 2006)

Enhancer of

Statement

Reference

p53^GMR.Ex is an enhancer of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Suppressor of

Statement

Reference

p53^GMR.Ex is a suppressor of eye phenotype of EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Corp^EY03495 under the control of Scer\GAL4^GMR.PF suppresses the increased cell death and reduced eye phenotypes caused by expression of p53^GMR.Ex.

Corp^95B enhances the increased cell death and reduced eye phenotypes caused by expression of p53^GMR.Ex.

(Chakraborty et al., 2015)

Induction of Ark^G8 third instar larval eyes disc clones blocks the cell death seen in p53^GMR.Ex mutants.

(Fan et al., 2010)

The p53^GMR.Ex-mediated induction of apoptosis in the posterior portion of late larval eye discs is reduced in hpo^42-47, wts^x1 and sav³ clones.

(Colombani et al., 2006)

The increased apoptosis in the eye disc caused by expression of p53^GMR.Ex is suppressed in a Nc^ΔA8/Nc^ΔA8 background, but the reduction in adult eye size caused by p53^GMR.Ex is not suppressed if the eye is made homozygous for Nc^ΔA8 (using the eyFLP method).

(Kondo et al., 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CDKN1A^GMR.PH rescues the cell death and R7 photoreceptor loss seen in p53^GMR.Ex third instar larval eye discs. The adult eye ablation phenotype is also rescued.

(Fan et al., 2010)

Complementation and Rescue Data

Rescued by

p53^GMR.Ex is rescued by p53^R155H.GMR.Ex

(Ollmann et al., 2000)

p53^GMR.Ex is rescued by p53^H159N.GMR.Ex

(Ollmann et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)