Mutant lin^G2 clones induced in the leg disc during the second instar result in shortening and fusion of all the proximal segments and the formation of deep invaginations in the leg cuticle of the adult. lin^G2 clones induced at early third instar result in shortening of the tarsus and fusion of tarsal segments. In both cases, adult leg size is much reduced. Mutant cells fail to contribute to inter-joint tissue or to differentiate inter-segmental bristles. Occasional out-pocketing of epithelial tissue decorated with smooth cuticle at inter-joint territories is observed.

Poorly differentiated adult antennae arise from lin^G2 mutant clone tissue induced during second instar.

In lin^G2/lin² embryos, the first three rows of the ventral denticle belts are replaced with smooth cuticle and denticle row 4 appears large like row 5. Excess denticles are produced posterior to the denticle belt. The anterior- and posterior-most en-expressing cells in the parasegment differentiate the 1^o dorsal cuticle type (dorsal denticles) in lin^G2/lin² embryos, while 2 to 3 cell rows internal to the en expression domain differentiate smooth cuticle. The anterior- and posterior-most en-expressing cells in the parasegment differentiate the 1^o dorsal cuticle type (dorsal denticles) in lin^G2/lin² embryos, while 2 to 3 cell rows internal to the en expression domain differentiate smooth cuticle. Expression of lin^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E in these embryos transforms all en-expressing cells to the 4^o fate (fine hairs). Expression of lin^Scer\UAS.cHa under the control of Scer\GAL4^wg.PM restores the 4^o fate, but only in the portion of the 4^o field that expresses wg.