Allele Dmel\ey^D1Da

General Information
Symbol	Dmel\ey^D1Da	Species	D. melanogaster
Name		FlyBase ID	FBal0117527
Feature type	allele	Associated gene	Dmel\ey

Map ( GBrowse )
Allele class
Mutagen	diepoxybutane
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	diepoxybutane (Callaerts et al., 2001, Benassayag et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References sequence variant 4:740,995..741,031 evidence=experimental comment=A region of 37bp is deleted and replaced by 5bp (GTCGG). This results in a frameshift and premature translation termination. A protein of 682 amino acids is produced, the last 29 of which are unrelated to wild type ey sequence. comment=@complex_change_in_nucleotide_sequence ; SO:1000005 comment=@plus_1_frameshift_mutation ; SO:1000066 (Callaerts et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference ey^D1Da encodes a peptide truncated at amino acid 682. (Benassayag et al., 2003) 37bp deletion (from position 2051 to 2080) is replaced by 5bp (GTCGG) in exon 9. This results in a frameshift and premature translation termination at position 2170. The predicted protein produced is 682 amino acids with the last 29 being unrelated to wild-type ey sequence. (Callaerts et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size (with ey^JD) (Clements et al., 2008) lethal \| partially (with Df(4)J2) (Callaerts et al., 2001) lethal \| partially \| recessive (Callaerts et al., 2001) locomotor behavior defective (Callaerts et al., 2001) neuroanatomy defective (Callaerts et al., 2001) neuroanatomy defective (with Df(4)J2) (Callaerts et al., 2001) neuroanatomy defective (with ey^J5.71) (Callaerts et al., 2001) small body (with ey^JD) (Clements et al., 2008) viable (with ey²) (Callaerts et al., 2001) viable (with ey^J5.71) (Callaerts et al., 2001) viable (with ey^R) (Callaerts et al., 2001) visible (Niimi et al., 2002, Callaerts et al., 2001) visible (with Df(4)J2) (Callaerts et al., 2001) visible (with ey^J5.71) (Callaerts et al., 2001) visible (with ey^OK107-4) (Clements et al., 2009) visible (with ey^OK107-6) (Clements et al., 2009) visible (with ey^OK107-10) (Clements et al., 2009) visible (with ey^OK107-16) (Clements et al., 2009) visible (with ey^R) (Callaerts et al., 2001)
Phenotype Manifest In
	adult brain (Callaerts et al., 2001) adult central complex (Callaerts et al., 2001) adult central complex (with Df(4)J2) (Callaerts et al., 2001) adult central complex (with ey^J5.71) (Callaerts et al., 2001) adult central complex (with ey^OK107-4) (Clements et al., 2009) adult central complex (with ey^OK107-6) (Clements et al., 2009) adult central complex (with ey^OK107-10) (Clements et al., 2009) adult central complex (with ey^OK107-16) (Clements et al., 2009) adult mushroom body (Callaerts et al., 2001) adult mushroom body (with Df(4)J2) (Callaerts et al., 2001) adult mushroom body (with ey^J5.71) (Callaerts et al., 2001) adult mushroom body (with ey^OK107-4) (Clements et al., 2009) adult mushroom body (with ey^OK107-6) (Clements et al., 2009) adult mushroom body (with ey^OK107-10) (Clements et al., 2009) adult mushroom body (with ey^OK107-16) (Clements et al., 2009) adult pars intercerebralis (with ey^JD) (Clements et al., 2008) cortex of medulla (Callaerts et al., 2001) ellipsoid body (Niimi et al., 2002, Callaerts et al., 2001) eye (Niimi et al., 2002, Callaerts et al., 2001) eye (with Df(4)J2) (Callaerts et al., 2001) eye (with ey^J5.71) (Callaerts et al., 2001) eye (with ey^OK107-4) (Clements et al., 2009) eye (with ey^OK107-6) (Clements et al., 2009) eye (with ey^OK107-10) (Clements et al., 2009) eye (with ey^OK107-16) (Clements et al., 2009) eye (with ey^R) (Callaerts et al., 2001) fan-shaped body (Callaerts et al., 2001) lamina (Callaerts et al., 2001) lobe system of mushroom body (Callaerts et al., 2001) lobula (Callaerts et al., 2001) lobula plate (Callaerts et al., 2001) median bundle (with ey^JD) (Clements et al., 2008) medulla (Callaerts et al., 2001) mushroom body (Martini et al., 2000) mushroom body & neuron (Martini et al., 2000) mushroom body calyx (Callaerts et al., 2001) neurite (with ey^JD) (Clements et al., 2008) optic lobe (Callaerts et al., 2001) protocerebral bridge (Callaerts et al., 2001) serpentine layer (Callaerts et al., 2001) wing (with ey^JD) (Clements et al., 2008)
Detailed Description
	Statement Reference ey[D1Da]/ey[JD] mutant flies are smaller in size than wild-type. Wing size is reduced by approximately 10%, and the average weight of mutant flies is between 66-68% of wild-type body weight. In ey[D1Da]/ey[JD] mutant brains, the morphology of the insulin producing cells (IPCs) is abnormal. Clustering of the IPC somata is disrupted in mutants, with disorganisation of the IPC cluster and reduced cell size. Overgrowth of IPC neurites along the dorsal-most periphery of the brain is frequently observed. The IPC axonal projections exhibit morphological defects. Proximal to the somata, the median bundle is thinner than in controls. Further distal, the median bundle displays a prominent defasciculation phenotype in which axons defasciculate from the median bundle as they project ventrally. (Clements et al., 2008) Homozygotes have reduced eyes. The ellipsoid body of the adult brain is disrupted. (Niimi et al., 2002) Homozygotes show 13% lethality. ey^D1Da/Df(4)J2 flies show 29% lethality. Eyes are reduced in size in homozygotes; 14% have eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50% of wild-type size, 72% have eyes that are 50-75% of wild-type size and 0% have eyes that are 75-100% of wild-type size. Homozygous adults have smaller brains than normal, with defects in the optic lobes, the central complex and the mushroom bodies. In the optic lobe, the lamina is often smaller and flatter than normal, the medulla is reduced in size and mispositioned, the medulla cortex is severely underdeveloped and the serpentine layer (which separates the distal and proximal part of the medulla) is disorganised. The lobula and lobula plate are reduced in size, the internal chiasm is abnormal and ectopic fibre bundles which appear to originate directly from the lamina are seen in the lobula/lobula plate complex. Fusion of the optic lobe with the central brain is occasionally seen. The mushroom body lobes are generally not recognisable in homozygous flies, while peduncles are seen in most brains, but they are greatly reduced in diameter. The calyces of the mushroom bodies are reduced in size. In the central complex, the ellipsoid body appears to be fused to the fan-shaped body and both these neuropils are not well separated from the surrounding protocerebral neuropil. The protocerebral bridge is disintegrated into several chunks of neuropil. The total volume of the central complex is almost unchanged compared to wild type. Abnormal neuronal projections are seen in the ellipsoid and fan-shaped bodies. Homozygous flies are extremely reluctant to walk, both in "Buridan's paradigm" and a stepping analyser experiment. Eyes are reduced in size in ey^D1Da/Df(4)J2 flies; 31% have eyes that are 0-25% of wild-type size, 25% have eyes that are 25-50% of wild-type size, 43% have eyes that are 50-75% of wild-type size and 1% have eyes that are 75-100% of wild-type size. ey^D1Da/Df(4)J2 flies show severe mushroom body and central complex defects. 100% of ey²/ey^D1Da flies have eyes which are 75-100% of wild-type size and they have no overt defects in the mushroom body or central complex. 89% of ey^R/ey^D1Da flies have eyes which are 75-100% of wild-type size, 9% have eyes that are 50-75% of wild-type size, 1% have eyes that are 25-50% of wild-type size and 1% have eyes that are 0-25% of wild-type size. The flies have no overt defects in the mushroom body or central complex. Eyes are reduced in size in ey^D1Da/ey^J5.71 flies; 43% have eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50% of wild-type size, 38% have eyes that are 50-75% of wild-type size and 5% have eyes that are 75-100% of wild-type size. ey^D1Da/ey^J5.71 flies show severe mushroom body and central complex defects. (Callaerts et al., 2001) The number of mushroom body neurons and the size of the mushroom body neuropil is reduced in homozygous adults. (Martini et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference ey[+]/ey^D1Da is an enhancer of visible phenotype of pb^Rev3.HSPB (Callaerts et al., 2001)
Phenotype Manifest In
Enhancer of
Statement Reference ey[+]/ey^D1Da is an enhancer of eye phenotype of pb^Rev3.HSPB (Callaerts et al., 2001) ey^D1Da is an enhancer of eye phenotype of pb^Rev3.HSPB (Benassayag et al., 2003)
NOT Enhancer of
Statement Reference ey[+]/ey^D1Da is a non-enhancer of mushroom body phenotype of dac^P (Martini et al., 2000) ey[+]/ey^D1Da is a non-enhancer of phenotype of dac^P (Martini et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference ey^D1Da dominantly enhances the eye loss phenotype of pb^Rev3.HSPB flies. (Callaerts et al., 2001) dac¹; ey^D1Da, dac³; ey^D1Da and Df(2L)dac-4; ey^D1Da double heterozygotes have normal mushroom bodies and eyes. (Martini et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ey^OK107-4 (Clements et al., 2009) ey^OK107-6 (Clements et al., 2009) ey^OK107-10 (Clements et al., 2009) ey^OK107-16 (Clements et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Allelic series based on mutant phenotype; ey¹¹ > ey^D1Da > ey^EH > ey^JD. (Benassayag et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ey^D1 (Benassayag et al., 2003) ey^D1Da (Clements et al., 2009, Clements et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Clements et al., 2009, Dev. Biol. 334(2): 503--512 Mutational analysis of the eyeless gene and phenotypic rescue reveal that an intact Eyeless protein is necessary for normal eye and brain development in Drosophila. [FBrf0209021] Clements et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(42): 16183--16188 Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons. [FBrf0206198] Benassayag et al., 2003, Development 130(3): 575--586 Evidence for a direct functional antagonism of the selector genes proboscipedia and eyeless in Drosophila head development. [FBrf0155717] Niimi et al., 2002, genesis 34(1-2): 74--75 Dominant-negative form of the Pax6 homolog eyeless for tissue-specific loss-of-function studies in the developing eye and brain in Drosophila. [FBrf0152381] Callaerts et al., 2001, J. Neurobiol. 46(2): 73--88 Drosophila Pax-6/eyeless is essential for normal adult brain structure and function. [FBrf0132376] Martini et al., 2000, Development 127(12): 2663--2672 The retinal determination gene, dachshund, is required for mushroom body cell differentiation. [FBrf0128567]

Allele Dmel\eyD1Da

Allele Dmel\ey^D1Da