|Feature type||allele||Associated gene||Dmel\ey|
|Map ( GBrowse )|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
comment=A region of 37bp is deleted and replaced by 5bp (GTCGG). This results in a frameshift and premature translation termination. A protein of 682 amino acids is produced, the last 29 of which are unrelated to wild type ey sequence.
comment=@complex_change_in_nucleotide_sequence ; SO:1000005
comment=@plus_1_frameshift_mutation ; SO:1000066
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
mushroom body & neuron
ey[D1Da]/ey[JD] mutant flies are smaller in size than wild-type. Wing size is reduced by approximately 10%, and the average weight of mutant flies is between 66-68% of wild-type body weight. In ey[D1Da]/ey[JD] mutant brains, the morphology of the insulin producing cells (IPCs) is abnormal. Clustering of the IPC somata is disrupted in mutants, with disorganisation of the IPC cluster and reduced cell size. Overgrowth of IPC neurites along the dorsal-most periphery of the brain is frequently observed. The IPC axonal projections exhibit morphological defects. Proximal to the somata, the median bundle is thinner than in controls. Further distal, the median bundle displays a prominent defasciculation phenotype in which axons defasciculate from the median bundle as they project ventrally.
Homozygotes have reduced eyes. The ellipsoid body of the adult brain is disrupted.
Homozygotes show 13% lethality. eyD1Da/Df(4)J2 flies show 29% lethality. Eyes are reduced in size in homozygotes; 14% have eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50% of wild-type size, 72% have eyes that are 50-75% of wild-type size and 0% have eyes that are 75-100% of wild-type size. Homozygous adults have smaller brains than normal, with defects in the optic lobes, the central complex and the mushroom bodies. In the optic lobe, the lamina is often smaller and flatter than normal, the medulla is reduced in size and mispositioned, the medulla cortex is severely underdeveloped and the serpentine layer (which separates the distal and proximal part of the medulla) is disorganised. The lobula and lobula plate are reduced in size, the internal chiasm is abnormal and ectopic fibre bundles which appear to originate directly from the lamina are seen in the lobula/lobula plate complex. Fusion of the optic lobe with the central brain is occasionally seen. The mushroom body lobes are generally not recognisable in homozygous flies, while peduncles are seen in most brains, but they are greatly reduced in diameter. The calyces of the mushroom bodies are reduced in size. In the central complex, the ellipsoid body appears to be fused to the fan-shaped body and both these neuropils are not well separated from the surrounding protocerebral neuropil. The protocerebral bridge is disintegrated into several chunks of neuropil. The total volume of the central complex is almost unchanged compared to wild type. Abnormal neuronal projections are seen in the ellipsoid and fan-shaped bodies. Homozygous flies are extremely reluctant to walk, both in "Buridan's paradigm" and a stepping analyser experiment. Eyes are reduced in size in eyD1Da/Df(4)J2 flies; 31% have eyes that are 0-25% of wild-type size, 25% have eyes that are 25-50% of wild-type size, 43% have eyes that are 50-75% of wild-type size and 1% have eyes that are 75-100% of wild-type size. eyD1Da/Df(4)J2 flies show severe mushroom body and central complex defects. 100% of ey2/eyD1Da flies have eyes which are 75-100% of wild-type size and they have no overt defects in the mushroom body or central complex. 89% of eyR/eyD1Da flies have eyes which are 75-100% of wild-type size, 9% have eyes that are 50-75% of wild-type size, 1% have eyes that are 25-50% of wild-type size and 1% have eyes that are 0-25% of wild-type size. The flies have no overt defects in the mushroom body or central complex. Eyes are reduced in size in eyD1Da/eyJ5.71 flies; 43% have eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50% of wild-type size, 38% have eyes that are 50-75% of wild-type size and 5% have eyes that are 75-100% of wild-type size. eyD1Da/eyJ5.71 flies show severe mushroom body and central complex defects.
The number of mushroom body neurons and the size of the mushroom body neuropil is reduced in homozygous adults.
|Phenotype Manifest In|
|NOT Enhancer of|
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 6 )|