FlyBase Allele Report: Dmel\eIF4E1[UAS.cRa]

General Information

Symbol

Dmel\eIF4E1^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Rivera-Pomar

FlyBase ID

FBal0117540

Feature type

allele

Associated gene

Dmel\eIF4E1

Associated Insertion(s)

Carried in Construct

P{UAS-eIF4E1.R}

Also Known As

UAS-eIF4E, UAS-eIF-4E

Key Links

Transgenic product class

wild_type

(Sigrist et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sigrist et al., 2000)

Mutagen

in vitro construct

(Sigrist et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-eIF4E1.R}

Cytology

Description

UAS regulatory sequences drive expression of eIF4E1.

(Sigrist et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

eIF4E1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 6

modeled by Pink1^RNAi.UAS.cYa

(Liu and Lu, 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

abnormal neuroanatomy, with Scer\GAL4^{Mhc.Switch.PO}

(Menon et al., 2004)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Imai et al., 2008)

abnormal neurophysiology, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

chemical sensitive, with Scer\GAL4^da.G32

(Imai et al., 2008)

Phenotype Manifest In

bouton, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

dopaminergic neuron, with Scer\GAL4^ple.PF

(Imai et al., 2008)

embryonic/larval neuromuscular junction, with Scer\GAL4^{Mhc.Switch.PO}

(Menon et al., 2004)

neuromuscular junction, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

type I bouton, with Scer\GAL4^{Mhc.Switch.PO}

(Menon et al., 2004)

Detailed Description

Statement

Reference

Flies expressing Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 are more sensitive to hydrogen peroxide compared to control flies.

60 day old flies expressing eIF-4E^Scer\UAS.cRa under the control of Scer\GAL4^ple.PF show a significant reduction in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster compared to control flies

(Imai et al., 2008)

Overexpression of eIF-4E^Scer\UAS.cRa in muscle 6/7 under the control of Scer\GAL4^{Mhc.Switch.PO} produces an increase in the number of type 1b boutons compared to controls.

(Menon et al., 2004)

The mean amplitude of miniature postsynaptic currents (mEJCs) at the neuromuscular junction is indistinguishable from wild type in flies expressing eIF-4E^Scer\UAS.cRa under the control of Scer\GAL4^Mhc.PW. Evoked postsynaptic currents (eEJCs) are significantly larger than in wild-type. The number of junctional boutons per neuromuscular junction is increased compared to wild type.

(Sigrist et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ple.PF, eIF4E1^UAS.cRa has abnormal neuroanatomy | adult stage phenotype, suppressible by Lrrk^unspecified/Lrrk^unspecified

(Imai et al., 2008)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

eIF4E1^UAS.cRa, Scer\GAL4^ptc.PU is a non-suppressor of abnormal size | adult stage phenotype of CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

eIF4E1^UAS.cRa, Scer\GAL4^ptc.PU is a non-suppressor of abnormal size | adult stage phenotype of CCT4^NIG.5525R, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ple.PF, eIF4E1^UAS.cRa has dopaminergic neuron phenotype, suppressible by Lrrk^unspecified/Lrrk^unspecified

(Imai et al., 2008)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has NMJ bouton phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has type I bouton phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has embryonic/larval neuromuscular junction phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

eIF4E1^UAS.cRa, Scer\GAL4^ptc.PU is a non-suppressor of wing blade phenotype of CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

eIF4E1^UAS.cRa, Scer\GAL4^ptc.PU is a non-suppressor of wing blade phenotype of CCT4^NIG.5525R, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing eIF-4E^Scer\UAS.cRa under the control of Scer\GAL4^ple.PF is suppressed if the flies are also homozygous for Lrrk^unspecified.

(Imai et al., 2008)

Simultaneous expression of pum^Scer\UAS.fl and eIF-4E^Scer\UAS.cRa in muscles (using Scer\GAL4^{Mhc.Switch.PO}) results in the increase in 1b bouton number conferred by overexpressing eIF-4E^Scer\UAS.cRa being completely suppressed by the overexpression of pum^Scer\UAS.fl. 1b bouton numbers in larvae overexpressing both eIF-4E^Scer\UAS.cRa and pum^Scer\UAS.fl are not significantly different from those in larvae overexpressing only pum^Scer\UAS.fl.

(Menon et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8650

w^*; P{UAS-eIF4E1.R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

eIF-4E^Scer\UAS.cRa

eIF4E1^Scer\UAS.cRa

eIF4E1^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Rivera-Pomar

(Sigrist et al., 2000)

Secondary FlyBase IDs

References (6)

Report Sections

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