FlyBase Allele Report: Dmel\Cdk2[3]

General Information

Symbol

Dmel\Cdk2³

Species

D. melanogaster

Name

FlyBase ID

FBal0117571

Feature type

allele

Associated gene

Dmel\Cdk2

Associated Insertion(s)

Carried in Construct

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lane et al., 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3R:20,736,416..20,736,416 [+]

Nucleotide change:

C20736416T

Amino acid change:

Q134term | Cdk2-PA; Q134term | Cdk2-PB; Q134term | Cdk2-PC

Reported amino acid change:

Q134term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lane et al., 2000)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: Q134term.

(Lane et al., 2000)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Park et al., 2017)

decreased body size | larval stage (with Cdk2¹)

(Lane et al., 2000)

decreased body size | larval stage (with Cdk2²)

(Lane et al., 2000)

lethal | recessive

(Lane et al., 2000)

Phenotype Manifest In

axon | third instar larval stage

(Park et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage

(Park et al., 2017)

Detailed Description

Statement

Reference

Cdk2³ homozygous (and with a lower penetrance also heterozygous) mutant third instar larvae display high frequency of ectopic targetting of type III axons to the abdominal body-wall muscle 13.

(Park et al., 2017)

Hemizygous embryos appear to have wild-type morphology. They incorporate BrdU with normal efficiency and in normal patterns throughout embryogenesis. Hatching of larvae occurs at the same rate as that of control embryos.

(Lane et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cdk2³ has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by rl¹/rl⁺

(Park et al., 2017)

Enhancer of

Statement

Reference

Cdk2³/Cdk2[+] is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of rl¹

(Park et al., 2017)

NOT Enhancer of

Statement

Reference

Cdk2³/cdc2c[+] is a non-enhancer of visible phenotype of mi¹/mi^ΔEY22

(Szuplewski et al., 2009)

Suppressor of

Statement

Reference

Cdk2³/cdc2c[+] is a suppressor of visible | dominant phenotype of Snr1^E1

(Zraly et al., 2004)

Cdk2³/cdc2c[+] is a suppressor of visible phenotype of CycE^I.hs.2sev

(Lane et al., 2000)

NOT Suppressor of

Statement

Reference

Cdk2³/cdc2c[+] is a non-suppressor of visible phenotype of mi¹/mi^ΔEY22

(Szuplewski et al., 2009)

Other

Statement

Reference

CycD¹, Cdk2³ has lethal | dominant phenotype

(Emmerich et al., 2004)

CycD¹, Cdk2³/cdc2c[+] has lethal phenotype

(Emmerich et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdk2³ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by rl¹/rl⁺

(Park et al., 2017)

Cdk2³ has axon | third instar larval stage phenotype, enhanceable by rl¹/rl⁺

(Park et al., 2017)

Enhancer of

Statement

Reference

Cdk2³/Cdk2[+] is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of rl¹

(Park et al., 2017)

Cdk2³/Cdk2[+] is an enhancer of axon | third instar larval stage phenotype of rl¹

(Park et al., 2017)

NOT Enhancer of

Statement

Reference

Cdk2³/cdc2c[+] is a non-enhancer of macrochaeta & thorax phenotype of mi¹/mi^ΔEY22

(Szuplewski et al., 2009)

Suppressor of

Statement

Reference

Cdk2³/cdc2c[+] is a suppressor of wing vein | ectopic phenotype of Snr1^E1

(Zraly et al., 2004)

Cdk2³/cdc2c[+] is a suppressor of wing phenotype of Snr1^E1

(Zraly et al., 2004)

Cdk2³/cdc2c[+] is a suppressor of eye phenotype of CycE^I.hs.2sev

(Lane et al., 2000)

Cdk2³/cdc2c[+] is a suppressor of ommatidium phenotype of CycE^I.hs.2sev

(Lane et al., 2000)

NOT Suppressor of

Statement

Reference

Cdk2³/cdc2c[+] is a non-suppressor of macrochaeta & thorax phenotype of mi¹/mi^ΔEY22

(Szuplewski et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of aberrant type III axon innervation patterns in body-wall muscle 13 of third instar larvae single heterozygous for either Cdk2³ or rl¹ is mutually enhanced in the double heterozygotes.

(Park et al., 2017)

Heterozygosity for either cdc2c² or cdc2c³ has no effect on the small bristle phenotype of mi¹/mi^ΔEY22 flies.

(Szuplewski et al., 2009)

Adult viability of CycD¹/Y ; cdc2c³/+ males is 0%.

(Emmerich et al., 2004)

At 29^oC, cdc2c³/+ suppresses the ectopic wing vein phenotype of Snr1^E1/+; 100% of the flies have wild-type wings.

(Zraly et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cdk2³/Cdk2² is rescued by Cdk2^hs.PS

(Lane et al., 2000)

Cdk2³/Df(3R)H⁸¹ is rescued by Cdk2^+t10

(Lane et al., 2000)

Cdk2³/Cdk2² is rescued by Cdk2^AF.hs

(Lane et al., 2000)

Comments

cdc2c¹/cdc2c³ and cdc2c²/cdc2c³ larvae survive for at least 4 days after egg deposition, but their size fails to increase significantly after 1.5 days (in contrast to wild-type larvae). cdc2c²/cdc2c³ females that have been rescued by periodic expression of cdc2c^hs.PS readily lay eggs as long as they are subjected to periodic heat shocks (to induce expression of cdc2c^hs.PS). However, after termination of periodic heat shocks, egg deposition decreases rapidly and stops completely within 2-3 days. This arrest of egg deposition can be readily reversed within 7 days after resumption of periodic heat shocks. Eggs collected from females 1 day after termination of periodic heat shocks often show abnormal spatial distribution of nuclei and the chromatin often appears abnormal. A significant fraction of embryos contain very few nuclei. About two-thirds of eggs with less than 5 nuclei are not fertilised. Periodic expression of cdc2c^hs.PS (using heat shock) in cdc2c²/cdc2c³ animals allows them to develop into morphologically normal and fertile adults. Some animals are still observed to develop into adults even if the onset of periodic expression of cdc2c^hs.PS is delayed until 116 hours after egg deposition.

(Lane et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6636

w^*; Cdk2³/TM3, Sb¹ P{35UZ}2

Notes on Origin

Discoverer

Selected as: a dominant modifier of the CycE^I.hs.2sev rough eye phenotype.

(Lane et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdk2³

(Hur et al., 2020, Park et al., 2017, Emmerich et al., 2004, Lane et al., 2000)

cdc2c³

(Szuplewski et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (8)

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