FlyBase Allele Report: Scer\GAL4[futsch-C380]

General Information

Symbol

Scer\GAL4^futsch-C380

Species

S. cerevisiae

Name

FlyBase ID

FBal0117656

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GAL4}C380

Carried in Construct

Also Known As

C380-GAL4, C380

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

X:1,411,763..1,411,763

(González-Gutiérrez et al., 2020, Patel et al., 2020, Kadas et al., 2019, Ashley et al., 2018, Azpurua et al., 2018, Koon et al., 2018, Kuklin et al., 2017, Colomb and Brembs, 2016, Nesler et al., 2016, Weiss and Littleton, 2016, Williams et al., 2016, Williams et al., 2016, Babic et al., 2015, Kairamkonda and Nongthomba, 2014, Kerr et al., 2014, Mishra-Gorur et al., 2014, Korkut et al., 2013, Nesler et al., 2013, Rohrbough et al., 2013, Timmerman et al., 2013, Vonhoff et al., 2013, Fuentes-Medel et al., 2012, Koles et al., 2012, Koon and Budnik, 2012, Pradhan et al., 2012, Vonhoff et al., 2012, Freeman et al., 2011, Inada et al., 2011, Koon et al., 2011, Boerner and Duch, 2010, Freeman et al., 2010, Repnikova et al., 2010, Vonhoff and Duch, 2010, Lee et al., 2009, Pulver et al., 2009, Sanyal, 2009, Ataman et al., 2008, Duch et al., 2008, Hartwig et al., 2008, Parisky et al., 2008, Gorczyca et al., 2007, Li et al., 2007, Ataman et al., 2006, Ashley et al., 2005, Hodge et al., 2005, Mathew et al., 2005, Mathew et al., 2005, Sanyal et al., 2005, Ruiz-Canada et al., 2004, Wolfgang et al., 2004, Mathew et al., 2002, Packard et al., 2002, Roche et al., 2002, Koh et al., 1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Insertion components

P{GAL4}C380

Product class / Tool use(s)

Encoded product / tool

enhancer trap

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P5 -- pharate adult stage P15

indirect flight muscle motor neuron MN5

(Vonhoff et al., 2013)

larval stage

motor neuron

(Ruiz-Canada et al., 2004, Wolfgang et al., 2004, Mathew et al., 2002, Packard et al., 2002, Koh et al., 1999)

adult stage

motor neuron | subset

(Boerner and Duch, 2010)

indirect flight muscle motor neuron MN1

(Boerner and Duch, 2010)

indirect flight muscle motor neuron MN2

(Boerner and Duch, 2010)

indirect flight muscle motor neuron MN3

(Boerner and Duch, 2010)

indirect flight muscle motor neuron MN4

(Boerner and Duch, 2010)

indirect flight muscle motor neuron MN5

(Boerner and Duch, 2010)

third instar larval stage

ventral nerve cord

(Sanyal, 2009)

embryonic/larval brain

(Sanyal, 2009)

motor neuron

(Sanyal, 2009)

embryonic/larval optic lobe

(Sanyal, 2009)

RP2 motor neuron

(Sanyal, 2009)

aCC neuron

(Sanyal, 2009)

sensory neuron

(Sanyal, 2009)

larval multidendritic class IV neuron

(Sanyal, 2009)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

motor neuron

(Sanyal et al., 2003)

aCC neuron

(Sanyal et al., 2003)

RP2 motor neuron

(Sanyal et al., 2003)

RP3 motor neuron

(Sanyal et al., 2003)

RP5 motor neuron

(Sanyal et al., 2003)

Additional Information

Statement

Reference

Drives expression in motor neurons.

(FlyBase Curators, 2017)

Expressed in all motor neurons.

(Sanyal, 2009)

Scer\GAL4^futsch-C380 specifically labels five of six medial motor neurons, including aCC, RP2, RP3, RP5, and another contralateral neuron. Expression begins near the end of embryonic stage 17.

(Sanyal et al., 2003)

Expression is observed at the synaptic boutons of motor neurons.

(Packard et al., 2002)

Marker for

motor neuron

(Ruiz-Canada et al., 2004, Wolfgang et al., 2004, Mathew et al., 2002, Packard et al., 2002, Koh et al., 1999)

Reflects expression of

futsch

(Vonhoff et al., 2013, Sanyal, 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased fecundity | female, with Bx^GD1385

(Kairamkonda and Nongthomba, 2014)

female semi-sterile, with Bx^GD1385

(Kairamkonda and Nongthomba, 2014)

flight defective, with Hsap\MECP2^UAS.FL, Scer\GAL80^ChAT.3.3P

(Vonhoff et al., 2012)

flight defective, with Scer\GAL80^ChAT.3.3P, Sh^{EKO.UAS.GFP(GL)}

(Duch et al., 2008)

flightless, with Hsap\MECP2^R294X.UAS, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

increased cell death | adult stage, with Hsap\MECP2^{R294X.S80A.UAS}

(Williams et al., 2016)

increased cell death | pupal stage, with Hsap\MECP2^K431X.UAS

(Williams et al., 2016)

increased cell death | pupal stage, with Hsap\MECP2^R294X.UAS

(Williams et al., 2016)

increased cell death | pupal stage, with Hsap\MECP2^V312X.UAS, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

locomotor behavior defective | adult stage, with Adf1^{S64A.S184A.UAS.Tag:FLAG}

(Timmerman et al., 2013)

locomotor behavior defective | larval stage, with Adf1^{S64A.S184A.UAS.Tag:FLAG}

(Timmerman et al., 2013)

neuroanatomy defective, with aPKC^M.UAS

(Ruiz-Canada et al., 2004)

neuroanatomy defective, with Hsap\MECP2^UAS.FL, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016, Vonhoff et al., 2012)

neuroanatomy defective, with Mmmm\ralA^72L.UAS

(Koh et al., 2002)

neuroanatomy defective, with Nrx-1^UAS.cLa

(Li et al., 2007)

neuroanatomy defective, with Raf^UAS.F179

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^N17.UAS

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^UAS.cKa

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^V12.C40.UAS

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^V12.G37.UAS

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^V12.S35.UAS

(Koh et al., 2002)

neuroanatomy defective, with Ras85D^V12.UAS

(Koh et al., 2002)

neuroanatomy defective, with Scer\GAL80^ChAT.3.3P, kay^Fbz.UAS

(Hartwig et al., 2008)

neuroanatomy defective | heat sensitive, with TrpA1^UAS.(B).cKa

(Vonhoff et al., 2013)

neuroanatomy defective | third instar larval stage, with Bper\ptxA^act.UAS

(Koon and Budnik, 2012)

neuroanatomy defective | third instar larval stage, with Gαo^GD8640

(Koon and Budnik, 2012)

neuroanatomy defective | third instar larval stage, with Gαo^KK109018

(Koon and Budnik, 2012)

neuroanatomy defective | third instar larval stage, with Octβ1R^KK102153

(Koon and Budnik, 2012)

neuroanatomy defective | third instar larval stage, with Patr-1^UAS.cPa

(Pradhan et al., 2012)

neurophysiology defective, with Adf1^{S64A.S184A.UAS.Tag:FLAG}

(Timmerman et al., 2013)

neurophysiology defective, with Adf1^{S64A.S184A.UAS.Tag:FLAG}, Scer\GAL80^ChAT.3.3P

(Timmerman et al., 2013)

neurophysiology defective, with Crei\ChR2^UAS.cSa

(Ataman et al., 2008)

neurophysiology defective, with Fas2^UAS.cAa

(Ashley et al., 2005)

neurophysiology defective | adult stage, with Scer\GAL80^ChAT.3.3P, eag^DN.EKI.UAS

(Duch et al., 2008)

neurophysiology defective | adult stage, with Scer\GAL80^ChAT.3.3P, Sh^{EKO.UAS.GFP(GL)}

(Duch et al., 2008)

neurophysiology defective | larval stage

(Parisky et al., 2008)

neurophysiology defective | larval stage, with Atpα^D369N.UAS

(Parisky et al., 2008)

neurophysiology defective | larval stage, with sgg^A81T.UAS

(Ataman et al., 2008)

neurophysiology defective | larval stage, with sgg^UAS.cBa

(Ataman et al., 2008)

neurophysiology defective | larval stage, with Shaw^{tr.UAS.Tag:FLAG}

(Hodge et al., 2005)

neurophysiology defective | third instar larval stage, with jeb^UAS.cWa

(Rohrbough et al., 2013)

neurophysiology defective | third instar larval stage, with mir-289^UAS.mCherry

(Kuklin et al., 2017)

starvation stress response defective, with Octβ2R^GD2954

(Koon et al., 2011)

starvation stress response defective, with Octβ2R^KK109808

(Koon et al., 2011)

viable, with LamC^UAS.cSa

(Dialynas et al., 2010)

viable, with LamC^ΔN.UAS.cSa

(Dialynas et al., 2010)

Phenotype Manifest In

bouton, with aPKC^M.UAS

(Ruiz-Canada et al., 2004)

bouton, with Nrx-1^UAS.cLa

(Li et al., 2007)

bouton | supernumerary, with Mmmm\ralA^72L.UAS

(Koh et al., 2002)

bouton | supernumerary, with Raf^UAS.F179

(Koh et al., 2002)

bouton | supernumerary, with Ras85D^N17.UAS

(Koh et al., 2002)

bouton | supernumerary, with Ras85D^UAS.cKa

(Koh et al., 2002)

bouton | supernumerary, with Ras85D^V12.C40.UAS

(Koh et al., 2002)

bouton | supernumerary, with Ras85D^V12.UAS

(Koh et al., 2002)

bouton | supernumerary, with wg^{UAS.cHa.Tag:HA}

(Packard et al., 2002)

bouton | third instar larval stage, with Mad^GD4121

(Fuentes-Medel et al., 2012)

dendrite, with Hsap\MECP2^UAS.FL, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016, Vonhoff et al., 2012)

dendrite | heat sensitive, with TrpA1^UAS.(B).cKa

(Vonhoff et al., 2013)

embryonic/larval neuromuscular junction, with sgg^A81T.UAS

(Ataman et al., 2008)

embryonic/larval neuromuscular junction, with sgg^UAS.cBa

(Ataman et al., 2008)

embryonic/larval neuromuscular junction | third instar larval stage, with Mad^GD4121

(Fuentes-Medel et al., 2012)

embryonic/larval neuromuscular junction | third instar larval stage, with Octβ2R^GD2954

(Koon et al., 2011)

embryonic/larval neuromuscular junction | third instar larval stage, with Octβ2R^KK109808

(Koon et al., 2011)

embryonic/larval neuromuscular junction | third instar larval stage, with Patr-1^UAS.cPa

(Pradhan et al., 2012)

indirect flight muscle motor neuron MN5, with Hsap\MECP2^UAS.FL, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016, Vonhoff et al., 2012)

indirect flight muscle motor neuron MN5 | adult stage, with Hsap\MECP2^{R294X.S80A.UAS}

(Williams et al., 2016)

indirect flight muscle motor neuron MN5 | heat sensitive, with TrpA1^UAS.(B).cKa

(Vonhoff et al., 2013)

indirect flight muscle motor neuron MN5 | pupal stage, with Hsap\MECP2^K431X.UAS

(Williams et al., 2016)

indirect flight muscle motor neuron MN5 | pupal stage, with Hsap\MECP2^R294X.UAS

(Williams et al., 2016)

indirect flight muscle motor neuron MN5 | pupal stage, with Hsap\MECP2^V312X.UAS, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

microtubule & synapse, with aPKC^M.UAS

(Ruiz-Canada et al., 2004)

motor neuron, with Crei\ChR2^UAS.cSa

(Ataman et al., 2008)

motor neuron, with Scer\GAL80^ChAT.3.3P, kay^Fbz.UAS

(Hartwig et al., 2008)

motor neuron | larval stage, with Shaw^{tr.UAS.Tag:FLAG}

(Hodge et al., 2005)

neuromuscular junction, with Fas2^UAS.cAa

(Ashley et al., 2005)

neuromuscular junction, with mir-8^{sponge.UAS.EGFP}

(Nesler et al., 2013)

NMJ bouton, with Fas2^UAS.cAa

(Ashley et al., 2005)

NMJ bouton, with mir-8^{sponge.UAS.EGFP}

(Nesler et al., 2013)

NMJ bouton | larval stage | supernumerary

(Nesler et al., 2013)

NMJ bouton | larval stage | supernumerary, with mir-8^UAS.mCherry

(Nesler et al., 2013)

type I bouton | supernumerary, with Ras85D^V12.G37.UAS

(Koh et al., 2002)

type I bouton | supernumerary, with Ras85D^V12.S35.UAS

(Koh et al., 2002)

type I bouton | supernumerary | third instar larval stage, with Gαo^GD8640

(Koon and Budnik, 2012)

type I bouton | supernumerary | third instar larval stage, with Gαo^KK109018

(Koon and Budnik, 2012)

type I bouton | supernumerary | third instar larval stage, with Octβ1R^KK102153

(Koon and Budnik, 2012)

type I bouton | third instar larval stage, with Bper\ptxA^act.UAS

(Koon and Budnik, 2012)

type I bouton | third instar larval stage, with Octβ2R^GD2954

(Koon et al., 2011)

type I bouton | third instar larval stage, with Octβ2R^KK109808

(Koon et al., 2011)

type Ib terminal bouton | third instar larval stage, with Patr-1^UAS.cPa

(Pradhan et al., 2012)

type II bouton | supernumerary | third instar larval stage, with Gαo^KK109018

(Koon and Budnik, 2012)

type II bouton | third instar larval stage, with Bper\ptxA^act.UAS

(Koon and Budnik, 2012)

type II bouton | third instar larval stage, with Octβ2R^GD2954

(Koon et al., 2011)

type II bouton | third instar larval stage, with Octβ2R^KK109808

(Koon et al., 2011)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has neuroanatomy defective phenotype, suppressible | partially by kibra^GD7772, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Hsap\MECP2^R294X.UAS, Scer\GAL4^futsch-C380 has increased cell death | pupal stage phenotype, suppressible | partially by slp1^UAS.cUa

(Williams et al., 2016)

Hsap\MECP2^R294X.UAS, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has flightless phenotype, suppressible | partially by slp1^UAS.cUa

(Williams et al., 2016)

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has neuroanatomy defective phenotype, suppressible | partially by osa⁰⁰⁰⁹⁰/osa[+]

(Vonhoff et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL80^ChAT.3.3P, kibra^GD7772, Scer\GAL4^futsch-C380 is a suppressor | partially of neuroanatomy defective phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)} is a suppressor of neuroanatomy defective | larval stage phenotype of SERCA^Kum170, comt⁶

(Freeman et al., 2010)

Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)} is a suppressor of neurophysiology defective | larval stage phenotype of SERCA^Kum170, comt⁶

(Freeman et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Appl^sd.UAS, Scer\GAL4^futsch-C380 has NMJ bouton phenotype, enhanceable by Fas2^UAS.cAa/Scer\GAL4^BG487

(Ashley et al., 2005)

Suppressed by

Statement

Reference

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has indirect flight muscle motor neuron MN5 phenotype, suppressible | partially by kibra^GD7772, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has dendrite phenotype, suppressible | partially by kibra^GD7772, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Hsap\MECP2^R294X.UAS, Scer\GAL4^futsch-C380 has indirect flight muscle motor neuron MN5 | pupal stage phenotype, suppressible | partially by slp1^UAS.cUa

(Williams et al., 2016)

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has indirect flight muscle motor neuron MN5 phenotype, suppressible | partially by osa⁰⁰⁰⁹⁰/osa[+]

(Vonhoff et al., 2012)

Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P has dendrite phenotype, suppressible | partially by osa⁰⁰⁰⁹⁰/osa[+]

(Vonhoff et al., 2012)

Enhancer of

Statement

Reference

Scer\GAL4^futsch-C380/Appl^sd.UAS is an enhancer of NMJ bouton phenotype of Fas2^UAS.cAa, Scer\GAL4^BG487

(Ashley et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL80^ChAT.3.3P, kibra^GD7772, Scer\GAL4^futsch-C380 is a suppressor | partially of dendrite phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Scer\GAL80^ChAT.3.3P, kibra^GD7772, Scer\GAL4^futsch-C380 is a suppressor | partially of indirect flight muscle motor neuron MN5 phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

(Williams et al., 2016)

Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)} is a suppressor of neuromuscular junction | larval stage phenotype of SERCA^Kum170, comt⁶

(Freeman et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^futsch-C380/aPKC^M.UAS is a non-suppressor of bouton phenotype of futsch^K68

(Ruiz-Canada et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

wg^l-12/Scer\GAL4^futsch-C380 rescues wg^UAS.cHa

(Ataman et al., 2008)

wg^UAS.cHa/Scer\GAL4^futsch-C380 rescues wg^l-12

(Ataman et al., 2008)

Scer\GAL4^futsch-C380/Nrx-1^UAS.cLa rescues Nrx-1²⁷³/Df(3R)5C1

(Li et al., 2007)

wg^{UAS.cHa.Tag:HA}/Scer\GAL4^futsch-C380 rescues wg^l-12

(Packard et al., 2002)

Scer\GAL4^futsch-C380/scrib^UAS.cBa rescues scrib¹

(Roche et al., 2002)

Scer\GAL4^futsch-C380/scrib^UAS.cBa rescues scrib⁶⁷³

(Roche et al., 2002)

Partially rescues

Scer\GAL4^futsch-C380/Gαs^UAS.cCa partially rescues Gαs^B19

(Wolfgang et al., 2004)

Fails to rescue

Scer\GAL4^futsch-C380/Hsap\MECP2^UAS.FL fails to rescue Hsap\MECP2^R294X.UAS

(Williams et al., 2016)

Scer\GAL4^futsch-C380/Syx18^UAS.cGa fails to rescue Syx18^ex6

(Gorczyca et al., 2007)

Scer\GAL4^futsch-C380/scrib^UAS.cBa fails to rescue Df(3R)Tl-X/scrib⁶⁷³

(Roche et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80580

P{GAL4}C380, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^C380

Scer\GAL4^futsch-C380

Name Synonyms

Secondary FlyBase IDs

References (58)

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