Allele Dmel\Pten^dj189

General Information
Symbol	Dmel\Pten^dj189	Species	D. melanogaster
Name		FlyBase ID	FBal0117670
Feature type	allele	Created / Updated	2006-05-15/2006-05-15
Associated gene	Dmel\Pten

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The F-element is within a coding exon at the 5' end of the Pten gene. (Gao et al., 2000) The insertion site of the F-element is between position 3987 (a T) and 3988 (a C) as numbered for the rescue fragment in Pten^+t6. (Pan and Levis, 2001.9.10)
Assay mode
Caused by insertion	F{}Pten^dj189 (Pan and Levis, 2001.9.10, Gao et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number \| somatic clone (Gao et al., 2000) increased cell size \| somatic clone (Gao and Pan, 2001, Gao et al., 2000)
Phenotype Manifest In
	egg \| germ-line clone \| maternal effect (von Stein et al., 2005) pole cell \| germ-line clone \| maternal effect (von Stein et al., 2005) cleavage stage embryo \| germ-line clone \| maternal effect (von Stein et al., 2005) blastoderm embryo \| germ-line clone \| maternal effect (von Stein et al., 2005) gastrula embryo \| germ-line clone \| maternal effect (von Stein et al., 2005) nurse cell & actin cytoskeleton \| germ-line clone (von Stein et al., 2005) nurse cell \| germ-line clone (von Stein et al., 2005) nurse cell & nucleus \| germ-line clone (von Stein et al., 2005) eye \| somatic clone (Gao et al., 2000) eye photoreceptor cell \| somatic clone (Gao et al., 2000) ommatidium \| somatic clone (Gao et al., 2000) interommatidial bristle \| somatic clone (Gao et al., 2000) wing margin bristle \| somatic clone (Gao et al., 2000) eye disc \| somatic clone (Gao et al., 2000)
Detailed Description
	Statement Reference Homozygous mutant clones of Pten^dj189 in the eye produce ommatidia that are larger than wild-type and often bulge out of the eye. Eye bristles are also larger. Mutant photoreceptors are increased in area by about 140% compared to wild-type. Quantitative examination reveals that homozygous Pten^dj189 clones contain on average 3.2 times as many cells as their twin spot controls. The intervening non neuronal cells in these clones are probably increased in size. In clones that included the whole eye, the number of ommatidia increases from about 750 (in wild-type) to about 1075, the area of the eye increases by at least 55%. Clones in the wing margin leads to a smaller number of larger bristles along the margin. In embryos produced from germ-line clones of homozygous Pten^dj189 no increase in apoptosis is seen. (Gao et al., 2000) Homozygous embryos derived from homozygous female germline clones show severe abnormalities even in freshly laid eggs; the eggs are generally smaller and more roundish than wild type and many do not show any development. In those embryos that do initiate development, formation of pole cells is rarely seen, and where they do form, they are typically only 2 or 3 in number (compared to an average of 35 in wild type). In those homozygous embryos derived from homozygous female germline clones that do show development, axial expansion (the spreading out of nuclei along the long axis of the embryo in cycles 4-7) does not occur, resulting in a spherical rather than ellipsoid arrangement of nuclei prior to cortical migration and an abnormally low nuclear density in the posterior region of the embryo at the syncytial blastoderm stage. The synchrony of the cell cycle during cleavage divisions is lost (the pattern of mitoses is not completely randomised, but occurs in waves that always start at the posterior of the embryo). This results in blastoderm nuclei with different morphology and different condensation states of chromatin in distinct regions of the embryo at the beginning of cellularisation. The cellularisation front advances much more rapidly in the anterior of the mutant embryo compared to the posterior, and the delay in cellularisation at the posterior pole is often accompanied by severe defects in gastrulation, which often results in the rapid degeneration of the mutant embryo shortly after the onset of gastrulation. However, some mutant embryos recover well from the early developmental defects and complete embryogenesis without any gross morphological abnormalities. Survival beyond gastrulation is independent of whether the embryos receive a wild-type paternal copy of Pten. Stage 10 egg chambers in females containing homozygous germline clones have a very disorganised actin cytoskeleton which fills the nurse cell cytoplasm instead of localising to the cell borders. Fusion of nurse cells is often seen, as are mispositioned nurse cell nuclei that appear to have moved into the oocyte. (von Stein et al., 2005) Pten[dj189] mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Pten^dj189 has increased cell size \| somatic clone phenotype, non-enhanceable by Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Gao et al., 2000)
Suppressed by
Statement Reference Pten^dj189 has increased cell number \| somatic clone phenotype, suppressible by eIF-4a¹⁰⁰⁶ (Gao et al., 2000) Pten^dj189 has increased cell number \| somatic clone phenotype, suppressible \| partially by eIF-4a¹⁰⁶⁹ (Gao et al., 2000) Pten^dj189 has increased cell size \| somatic clone phenotype, suppressible by Akt1⁰⁴²²⁶ (Gao et al., 2000) Pten^dj189 has increased cell size \| somatic clone phenotype, suppressible \| partially by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
NOT suppressed by
Statement Reference Pten^dj189 has increased cell size \| somatic clone phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Gao et al., 2000) Pten^dj189 has increased cell size \| somatic clone phenotype, non-suppressible by eIF-4a¹⁰⁶⁹ (Gao et al., 2000) Pten^dj189 has increased cell size \| somatic clone phenotype, non-suppressible by InR³⁵/InR⁰⁵⁵⁴⁵ (Gao et al., 2000) Pten^dj189 has increased cell size phenotype, non-suppressible by Tap42^Δ305b (Cygnar, 2005)
NOT Enhancer of
Statement Reference Pten^dj189 is a non-enhancer of reduced cell size \| somatic clone phenotype of Tor^ΔP (Zhang et al., 2000)
NOT Suppressor of
Statement Reference Pten^dj189 is a non-suppressor of reduced cell size \| somatic clone phenotype of Tor^ΔP (Zhang et al., 2000)
Other
Statement Reference Pk61C⁵/Pk61C⁴, Pten^dj189/Pten^c494 has visible phenotype (Rintelen et al., 2001, Rintelen et al., 2001) Pk61C⁵/Pk61C⁴, Pten^dj189/Pten^c494 has viable phenotype (Rintelen et al., 2001, Rintelen et al., 2001)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Pten^dj189 has wing margin bristle phenotype, non-enhanceable by Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Gao et al., 2000)
Suppressed by
Statement Reference Pten^dj189 has wing margin bristle \| somatic clone phenotype, suppressible by Akt1⁰⁴²²⁶ (Gao et al., 2000)
NOT suppressed by
Statement Reference Pten^dj189 has wing margin bristle \| somatic clone phenotype, non-suppressible by InR³⁵/InR⁰⁵⁵⁴⁵ (Gao et al., 2000)
NOT Enhancer of
Statement Reference Pten^dj189 is a non-enhancer of cell cycle \| somatic clone phenotype of Tor^ΔP (Zhang et al., 2000)
NOT Suppressor of
Statement Reference Pten^dj189 is a non-suppressor of cell cycle \| somatic clone phenotype of Tor^ΔP (Zhang et al., 2000)
Other
Statement Reference Pk61C⁵/Pk61C⁴, Pten^dj189/Pten^c494 has adult abdomen phenotype (Rintelen et al., 2001, Rintelen et al., 2001) Pk61C⁵/Pk61C⁴, Pten^dj189/Pten^c494 has leg phenotype (Rintelen et al., 2001, Rintelen et al., 2001) Pten^dj189, eIF-4a¹⁰⁰⁶ has eye disc \| somatic clone phenotype (Gao et al., 2000, Gao et al., 2000) Pten^dj189, eIF-4a¹⁰⁶⁹ has eye disc \| somatic clone phenotype (Gao et al., 2000, Gao et al., 2000) Akt1¹/Akt1³, Pten^dj189/Pten¹¹⁷ has lipid particle & nurse cell phenotype (Vereshchagina and Wilson, 2006)
Additional Comments
Genetic Interactions
Statement Reference The addition of Pten^dj189 to Tor^ΔP mutant clones shows no effect on the Tor^ΔP phenotype. (Zhang et al., 2000) Pten^dj189, Tsc1²⁹ double mutant somatic clones show an additive cell size increase phenotype. (Gao and Pan, 2001) Pten^dj189/Pten^c494 ; Pk61C⁴/Pk61C⁵ animals can survive to adulthood, although the abdomen is unproportionally reduced in size and leg structures are deformed. (Rintelen et al., 2001) Clones of Pten^dj189 Tap42^Δ305b double mutant cells (homozygous for Pten^dj189 and Df(2L)Tap42^Δ305b and with the lack of Nnp-1 and CG6523 function caused by Df(2L)Tap42^Δ305b rescued using the P{Nnp-1^t5.1} transgene) show the characteristic increase in cell size and S/G₂ content of Pten^dj189 single mutants. (Cygnar, 2005) Pten[dj189] does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; Pten[dj189] clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36[+t4] (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, Pten[dj189] does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing. (Tyler et al., 2007) The nurse cells of Pten[dj189]/Pten[117]; Akt1[3]/Akt1[1] viable females show normal lipid storage, while clones of Pten alleles show formation of large lipid droplets. (Vereshchagina and Wilson, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Pten^MGH1 (Neufeld, 2001.1.11)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Identified during a FRT/FLP recombination screen for mutations affecting the development of adult cuticular structures. Mutagen not stated. (Gao et al., 2000)
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	dPTEN^dj189 (Stocker et al., 2002, Rintelen et al., 2001, Gao et al., 2000) dPTEN^DJ189 (Zhang et al., 2000) PTEN^DJ1 (Cygnar, 2005) Pten^dj189 (Vereshchagina and Wilson, 2006, ) Pten^DJ189 (von Stein et al., 2005) PTEN^dj189 (Reiling and Hafen, 2004, Gao and Pan, 2001, Tyler et al., 2007) PTEN^DJ189 (Mikeladze-Dvali et al., 2005) PTEN-DJ1 (Neufeld, 2001.1.11)
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Tyler et al., 2007, Genetics 175(2): 643--657 Genes affecting cell competition in Drosophila. [FBrf0192304] Vereshchagina and Wilson, 2006, Development 133(23): 4731--4735 Cytoplasmic activated protein kinase Akt regulates lipid-droplet accumulation in Drosophila nurse cells. [FBrf0193043] Coelho et al., 2005, Genetics 171(2): 597--614 A genetic screen for dominant modifiers of a small-wing phenotype in Drosophila melanogaster identifies proteins involved in splicing and translation. [FBrf0190712] Cygnar, 2005, Genetics 170(2): 733--740 The phosphatase subunit tap42 functions independently of target of rapamycin to regulate cell division and survival in Drosophila. [FBrf0188576] Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] von Stein et al., 2005, Development 132(7): 1675--1686 Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling. [FBrf0183928] Reiling and Hafen, 2004, Genes Dev. 18(23): 2879--2892 The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-regulating S6K activity upstream of TSC in Drosophila. [FBrf0180258] Stocker et al., 2002, Science 295(5562): 2088--2091 Living with lethal PIP3 levels: viability of flies lacking PTEN restored by a PH domain mutation in Akt/PKB. [FBrf0151483] Gao and Pan, 2001, Genes Dev. 15(11): 1383--1392 TSC1 and TSC2 tumor suppressors antagonize insulin signaling in cell growth. [FBrf0136941] Rintelen et al., 2001, Proc. Natl. Acad. Sci. USA 98(26): 15020--15025 PDK1 regulates growth through Akt and S6K in Drosophila. [FBrf0141724] Gao et al., 2000, Dev. Biol. 221(2): 404--418 Drosophila PTEN regulates cell growth and proliferation through PI3K-dependent and -independent pathways. [FBrf0127089] Zhang et al., 2000, Genes Dev. 14(21): 2712--2724 Regulation of cellular growth by the Drosophila target of rapamycin dTOR. [FBrf0131434]
Personal communication to FlyBase	Neufeld, 2001.1.11, PTEN-MGH1. PTEN-MGH1. [FBrf0133211] Pan and Levis, 2001.9.10, Tsc1<up>12</up>, Tsc1<up>+t4.7</up>, and Pten<up>dj189</up>. Tsc1<up>12</up>, Tsc1<up>+t4.7</up>, and Pten<up>dj189</up>. [FBrf0137847]

Allele Dmel\Ptendj189

Allele Dmel\Pten^dj189