eye, with Scer\GAL4ey.PH
wing, with Scer\GAL4P0206
Expression of PtenUAS.cGb under the control of the split-GAL4 pair of Scer\GAL4DBD.AkhR and Hsap\RELAAD.nSyb in adult females results in acceleration of starvation-induced hyperactivity, compared to controls.
Neuronal expression of PtenScer\UAS.cGb under the control of Scer\GAL4elav-C155 decreases sensitivity to ethanol sedation.
Neuronal expression of PtenScer\UAS.cGb (under the control of Scer\GAL4elav-C155) throughout development (until eclosion of the adult fly, when Scer\GAL80ts.αTub84B is induced) results in reduced ethanol sensitivity. The converse experiment, with over-expression in neurons only after eclosion, does not alter ethanol sensitivity.
Expression of PtenScer\UAS.cGb under the control of Scer\GAL4Lsp2.PH induces autolysosome formation in the fat body.
photoreceptor cell death is not detected in 30 day old flies expressing PtenScer\UAS.cGb under the control of Scer\GAL4GMR.PU exposed to light.
Flies expressing PtenScer\UAS.cGb under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 show a reduction in lifespan compared to controls.
Expression of PtenScer\UAS.cGb in the prothoracic gland and corpora allata, driven by Scer\GAL4P0206, suppresses the growth of both of these ring gland components. Scer\GAL4P0206>PtenScer\UAS.cGb flies have larger bodies than controls; this phenotype is first seen at the L2 stage, as evidenced by the length of the mouth hooks. As pharate adults, these flies are 42% heavier than controls. This phenotype continues into adulthood as can be seen by the unusually large adult wing areas. Scer\GAL4P0206>PtenScer\UAS.cGb larvae are developmentally delayed and molt to L2 later than controls. These larvae either molt to L3 later than controls or pupariate prematurely in the L2 stage. None of the L2 puparia eclose, although a few (<1%) develop to the pharate adult stage. Rearing Scer\GAL4P0206>PtenScer\UAS.cGb larvae on yeast paste enhances the amount of precocious L2 puparia, while rearing larvae on food containing 50% of the normal nutrients suppresses this phenotype, resulting in all larvae forming large L3 puparia. Expression of PtenScer\UAS.cGb in the prothoracic gland, driven by Scer\GAL4phm.PO, causes a prolonged L1 stage followed by death. Expression of PtenScer\UAS.cGb in the corpora allata, driven by Scer\GAL4Aug21, has no effect on pharate adult weight or on adult wing area.
Expression of PtenScer\UAS.cGb, under the control of Scer\GAL429BD, significantly reduces the size of the salivary gland in third instar larvae.
When fed ad libitum, third instar larvae expressing PtenScer\UAS.cGb, under the control of Scer\GAL4NPFR1.6.6 display hyperactive feeding on solid food, similar to control third instar larvae deprived of food for 40 minutes. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.
When PtenScer\UAS.cGb is driven by Scer\GAL4bun-Switch1.32 (in adults fed on mifepristone) the median lifespan can increased by as much as 20%.
When expression is driven in clones in the early L3 larval fat body increased autophagy occurs. When expression is driven by Scer\GAL4Lsp2.PH, relatively late in the larval fat body, no effect on autophagic activity of fat droplet aggregation is detectable.
Flies expressing PtenScer\UAS.cGb under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa, show a consistently high heart rate; in contrast to wild-type flies, this heart rate does not decrease with age. These flies also show a low stress-induced heart failure rate that does not increase with age, again in contrast to wild-type flies. These effects are true for both male and female flies.
PtenScer\UAS.cGb; Scer\GAL4GMR.PF animals have small eyes.
Ommatidial size in Scer\GAL4GMR.PF/+; PtenScer\UAS.cGb/+ flies is reduced to 0.69 of wild-type.
Expression of PtenScer\UAS.cGb under the control of Scer\GAL4Act5C.PP inhibits larval growth. Clones of cells expressing PtenScer\UAS.cGb under the control of Scer\GAL4Act5C.PP in the salivary glands and fat body are greatly reduced in size and have much smaller nuclei with far less DNA than adjacent control cells. Expression of PtenScer\UAS.cGb under the control of Scer\GAL4Adh.PF, Scer\GAL4en-e16E or Scer\GAL4Act5C.PP has no effect on viability under starvation conditions.
Expression of S6kScer\UAS.cWa under the control of Scer\GAL4ey.PH reduces eye size.
Expression of PtenScer\UAS.cGb driven by Scer\GAL4Bx-MS1096 produces wings of a dramatically reduced size. 18% of the reduction of wing size is due to a decrease in cell number, the rest is due to a decrease in cell size. Expression of PtenScer\UAS.cGb driven by Scer\GAL4en-e16E or Scer\GAL4ap-md544 causes a reduction in size in the posterior and dorsal compartments of the wing respectively. Expression of PtenScer\UAS.cGb driven by Scer\GAL4arm.PS causes lethality in almost all flies. Those flies that do survive show a reduction in body size.
PtenUAS.cGb, Scer\GAL4Bx-MS1096 has decreased cell number phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4Bx-MS1096 has decreased cell size phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4ey.PH is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4ey.PH, Tsc1UAS.cTa, gigUAS.cTa
PtenUAS.cGb, Scer\GAL4ey.PH is an enhancer of visible | adult stage phenotype of Scer\GAL4ey.PH, Tsc1UAS.cTa, gigUAS.cTa
PtenUAS.cGb, Scer\GAL4Bx-MS1096 has wing phenotype, enhanceable by Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4Lsp2.PH has fat body phenotype, suppressible by Acn27/Acn1
PtenUAS.cGb, Scer\GAL4Bx-MS1096 has wing phenotype, suppressible by Pi3K92EUAS.Tag:MYC, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4Bx-MS1096 has wing phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4ey.PH is an enhancer of eye phenotype of Scer\GAL4ey.PH, Tsc1UAS.cTa, gigUAS.cTa
PtenUAS.cGb, Scer\GAL4Bx-MS1096 is an enhancer of wing phenotype of Pi3K92EUAS.Tag:MYC, Scer\GAL4Bx-MS1096
PtenUAS.cGb, Scer\GAL4Lsp2.PH is a suppressor of lysosome phenotype of EcRB1-ΔC655.W650A.UAS, Scer\GAL4Lsp2.PH
PtenUAS.cGb, Scer\GAL4Lsp2.PH is a suppressor of autophagosome phenotype of EcRB1-ΔC655.W650A.UAS, Scer\GAL4Lsp2.PH
PtenUAS.cGb, Scer\GAL4GMR.PF is a non-suppressor of ommatidium phenotype of RhebEP50.084, Scer\GAL4GMR.PF
Expression of PtenScer\UAS.cGb under the control of Scer\GAL4Lsp2.PH cannot induce autolysosome formation in the fat body in a Acn1/Acn27 background.
Co-expression of PtenScer\UAS.cGb partially restores starvation-induced autophagy in larvae expressing FK506-bp1Scer\UAS.cJa under the control of Scer\GAL4Cg.PA.
The small eye phenotype of PtenScer\UAS.cGb; Scer\GAL4GMR.PF animals is suppressed by RhebScer\UAS.cSa. The resulting eyes are slightly rough.
The large size and disorganisation of ommatidia in Scer\GAL4GMR.PF/+; RhebEP50.084/+ flies is unaffected by PtenScer\UAS.cGb.
Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of PtenScer\UAS.cGb enhances the phenotype.
Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and PtenScer\UAS.cGb under the control of Scer\GAL4Bx-MS1096 have wings even smaller than flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC or PtenScer\UAS.cGb alone.
The addition of BacA\p35Scer\UAS.cHa does not suppress the wing phenotype seen in PtenScer\UAS.cGb,Scer\GAL4Bx-MS1096 flies. The addition of Pi3K92EScer\UAS.T:Hsap\MYC suppresses the wing phenotype seen in PtenScer\UAS.cGb,Scer\GAL4Bx-MS1096 flies. Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and PtenScer\UAS.cGb under the control of Scer\GAL4Bx-MS1096 have wings even smaller than flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC or PtenScer\UAS.cGb alone.
The addition of BacA\p35Scer\UAS.cHa does not suppress the wing phenotype seen in PtenScer\UAS.cGb,Scer\GAL4Bx-MS1096 flies. The addition of Pi3K92EScer\UAS.T:Hsap\MYC suppresses the wing phenotype seen in PtenScer\UAS.cGb,Scer\GAL4Bx-MS1096 flies.
