Allele Dmel\Pten^Scer\UAS.cGb

General Information
Symbol	Dmel\Pten^Scer\UAS.cGb	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Gao	FlyBase ID	FBal0117671
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\Pten

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Gao et al., 2000)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode	In transgenic Drosophila (intraspecific) (Gao et al., 2000, Saucedo et al., 2003, Wu et al., 2005)
Carried in construct	P{UAS-Pten.Gb} (Mirth et al., 2005, Stocker et al., 2003, Saucedo et al., 2003, Takeo et al., 2005, Britton et al., 2002, Wu et al., 2005, Szuplewski et al., 2003, Hwangbo et al., 2004, Gao et al., 2000, Scott et al., 2004, Rusten et al., 2004, Tapon et al., 2001, Mikeladze-Dvali et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type \| conditional RU486, with Scer\GAL4^Switch1.106 (Hwangbo et al., 2004) viable, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004) lethal \| partially \| pupal stage, with Scer\GAL4^P0206 (Mirth et al., 2005) lethal \| first instar larval stage, with Scer\GAL4^phm.PO (Mirth et al., 2005) lethal, with Scer\GAL4^arm.PS (Gao et al., 2000) visible, with Scer\GAL4^ey.PH (Tapon et al., 2001) small body, with Scer\GAL4^arm.PS (Gao et al., 2000) reduced cell number, with Scer\GAL4^Bx-MS1096 (Gao et al., 2000) reduced cell number, with Scer\GAL4^en-e16E (Gao et al., 2000) reduced cell number, with Scer\GAL4^ap-md544 (Gao et al., 2000) reduced cell size \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) reduced cell size, with Scer\GAL4^Bx-MS1096 (Gao et al., 2000) reduced cell size, with Scer\GAL4^en-e16E (Gao et al., 2000) reduced cell size, with Scer\GAL4^ap-md544 (Gao et al., 2000) large body \| second instar larval stage, with Scer\GAL4^P0206 (Mirth et al., 2005) large body \| pharate adult stage, with Scer\GAL4^P0206 (Mirth et al., 2005) aging defective, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004) long lived \| conditional RU486, with Scer\GAL4^{bun-Switch1.32} (Hwangbo et al., 2004) developmental rate defective \| second instar larval stage, with Scer\GAL4^P0206 (Mirth et al., 2005) developmental rate defective \| third instar larval stage, with Scer\GAL4^P0206 (Mirth et al., 2005)
Phenotype Manifest In
	prothoracic gland, with Scer\GAL4^P0206 (Mirth et al., 2005) larval corpus allatum, with Scer\GAL4^P0206 (Mirth et al., 2005) puparium, with Scer\GAL4^P0206 (Mirth et al., 2005) wing, with Scer\GAL4^P0206 (Mirth et al., 2005) eye, with Scer\GAL4^GMR.PF (Saucedo et al., 2003) ommatidium, with Scer\GAL4^GMR.PF (Stocker et al., 2003) eye, with Scer\GAL4^ey.PH (Tapon et al., 2001) wing, with Scer\GAL4^Bx-MS1096 (Gao et al., 2000) larval salivary gland \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) larval fat body \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) larval salivary gland, with Scer\GAL4^29BD (Takeo et al., 2005) wing \| posterior compartment, with Scer\GAL4^en-e16E (Gao et al., 2000) wing \| dorsal compartment, with Scer\GAL4^ap-md544 (Gao et al., 2000) adult heart, with Scer\GAL4^Scer\UAS.cHa, Scer\GAL4^tin.cBa (Wessells et al., 2004)
Detailed Description
	Statement Reference Expression of Pten^Scer\UAS.cGb driven by Scer\GAL4^Bx-MS1096 produces wings of a dramatically reduced size. 18% of the reduction of wing size is due to a decrease in cell number, the rest is due to a decrease in cell size. Expression of Pten^Scer\UAS.cGb driven by Scer\GAL4^en-e16E or Scer\GAL4^ap-md544 causes a reduction in size in the posterior and dorsal compartments of the wing respectively. Expression of Pten^Scer\UAS.cGb driven by Scer\GAL4^arm.PS causes lethality in almost all flies. Those flies that do survive show a reduction in body size. (Gao et al., 2000) Expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^ey.PH reduces eye size. (Tapon et al., 2001) Expression of Pten^Scer\UAS.cGb under the control of Scer\GAL4^Act5C.PP inhibits larval growth. Clones of cells expressing Pten^Scer\UAS.cGb under the control of Scer\GAL4^Act5C.PP in the salivary glands and fat body are greatly reduced in size and have much smaller nuclei with far less DNA than adjacent control cells. Expression of Pten^Scer\UAS.cGb under the control of Scer\GAL4^Adh.PF, Scer\GAL4^en-e16E or Scer\GAL4^Act5C.PP has no effect on viability under starvation conditions. (Britton et al., 2002) Pten^Scer\UAS.cGb; Scer\GAL4^GMR.PF animals have small eyes. (Saucedo et al., 2003) When Pten^Scer\UAS.cGb is driven by Scer\GAL4^{bun-Switch1.32} (in adults fed on mifepristone) the median lifespan can increased by as much as 20%. (Hwangbo et al., 2004) Ommatidial size in Scer\GAL4^GMR.PF/+; Pten^Scer\UAS.cGb/+ flies is reduced to 0.69 of wild-type. (Stocker et al., 2003) When expression is driven in clones in the early L3 larval fat body increased autophagy occurs. When expression is driven by Scer\GAL4^Lsp2.PH, relatively late in the larval fat body, no effect on autophagic activity of fat droplet aggregation is detectable. (Rusten et al., 2004) Flies expressing Pten^Scer\UAS.cGb under the control of both Scer\GAL4^tin.cBa and Scer\GAL4^Scer\UAS.cHa, show a consistently high heart rate; in contrast to wild-type flies, this heart rate does not decrease with age. These flies also show a low stress-induced heart failure rate that does not increase with age, again in contrast to wild-type flies. These effects are true for both male and female flies. (Wessells et al., 2004) Expression of Pten^Scer\UAS.cGb, under the control of Scer\GAL4^29BD, significantly reduces the size of the salivary gland in third instar larvae. (Takeo et al., 2005) When fed ad libitum, third instar larvae expressing Pten^Scer\UAS.cGb, under the control of Scer\GAL4^NPFR1.6.6 display hyperactive feeding on solid food, similar to control third instar larvae deprived of food for 40 minutes. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls. (Wu et al., 2005) Expression of Pten^Scer\UAS.cGb in the prothoracic gland and corpora allata, driven by Scer\GAL4^P0206, suppresses the growth of both of these ring gland components. Scer\GAL4^P0206>Pten^Scer\UAS.cGb flies have larger bodies than controls; this phenotype is first seen at the L2 stage, as evidenced by the length of the mouth hooks. As pharate adults, these flies are 42% heavier than controls. This phenotype continues into adulthood as can be seen by the unusually large adult wing areas. Scer\GAL4^P0206>Pten^Scer\UAS.cGb larvae are developmentally delayed and molt to L2 later than controls. These larvae either molt to L3 later than controls or pupariate prematurely in the L2 stage. None of the L2 puparia eclose, although a few (<1%) develop to the pharate adult stage. Rearing Scer\GAL4^P0206>Pten^Scer\UAS.cGb larvae on yeast paste enhances the amount of precocious L2 puparia, while rearing larvae on food containing 50% of the normal nutrients suppresses this phenotype, resulting in all larvae forming large L3 puparia. Expression of Pten^Scer\UAS.cGb in the prothoracic gland, driven by Scer\GAL4^phm.PO, causes a prolonged L1 stage followed by death. Expression of Pten^Scer\UAS.cGb in the corpora allata, driven by Scer\GAL4^Aug21, has no effect on pharate adult weight or on adult wing area. (Mirth et al., 2005) Scer\GAL4[GMR.long]>Pten[Scer\UAS.cGb] mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 has reduced cell number phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Bx-MS1096 (Gao et al., 2000) Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 has reduced cell size phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
Phenotype Manifest In
Enhanced by
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
Suppressed by
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
NOT suppressed by
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
Enhancer of
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 (Gao et al., 2000)
Suppressor of
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^Lsp2.PH is a suppressor of lysosome phenotype of EcR^{B1-ΔC655.W650A.Scer\UAS}, Scer\GAL4^Lsp2.PH (Rusten et al., 2004) Pten^Scer\UAS.cGb, Scer\GAL4^Lsp2.PH is a suppressor of autophagic vacuole phenotype of EcR^{B1-ΔC655.W650A.Scer\UAS}, Scer\GAL4^Lsp2.PH (Rusten et al., 2004)
NOT Suppressor of
Statement Reference Pten^Scer\UAS.cGb, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Rheb^EP50.084, Scer\GAL4^GMR.PF (Stocker et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The addition of BacA\p35^Scer\UAS.cHa does not suppress the wing phenotype seen in Pten^Scer\UAS.cGb,Scer\GAL4^Bx-MS1096 flies. The addition of Pi3K92E^{Scer\UAS.T:Hsap\MYC} suppresses the wing phenotype seen in Pten^Scer\UAS.cGb,Scer\GAL4^Bx-MS1096 flies. Flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} and Pten^Scer\UAS.cGb under the control of Scer\GAL4^Bx-MS1096 have wings even smaller than flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} or Pten^Scer\UAS.cGb alone. (Gao et al., 2000) Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Coexpression of Pten^Scer\UAS.cGb enhances the phenotype. (Tapon et al., 2001) The small eye phenotype of Pten^Scer\UAS.cGb; Scer\GAL4^GMR.PF animals is suppressed by Rheb^Scer\UAS.cSa. The resulting eyes are slightly rough. (Saucedo et al., 2003) The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is unaffected by Pten^Scer\UAS.cGb. (Stocker et al., 2003) Flies expressing Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] and Pten[Scer\UAS.cGb] under the control of Scer\GAL4[Bx-MS1096] have wings even smaller than flies expressing Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] or Pten[Scer\UAS.cGb] alone. (Gao et al., 2000)
Xenogenetic Interactions
Statement Reference The addition of BacA\p35[Scer\UAS.cHa] does not suppress the wing phenotype seen in Pten[Scer\UAS.cGb],Scer\GAL4[Bx-MS1096] flies. The addition of Pi3K92E[Scer\UAS.T:Hsap\MYC] suppresses the wing phenotype seen in Pten[Scer\UAS.cGb],Scer\GAL4[Bx-MS1096] flies. (Gao et al., 2000)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Pten^Scer\UAS.cGb
Name Synonym	Saccharomyces cerevisiae UAS construct a of Gao (Gao et al., 2000)
Secondary FlyBase IDs

References ( 14 )
Research paper	Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] Mirth et al., 2005, Curr. Biol. 15(20): 1796--1807 The role of the prothoracic gland in determining critical weight for metamorphosis in Drosophila melanogaster. [FBrf0190035] Takeo et al., 2005, Dev. Biol. 284(1): 204--218 Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. [FBrf0187400] Wu et al., 2005, Proc. Natl. Acad. Sci. USA 102(37): 13289--13294 Regulation of hunger-driven behaviors by neural ribosomal S6 kinase in Drosophila. [FBrf0188283] Hwangbo et al., 2004, Nature 429(6991): 562--566 Drosophila dFOXO controls lifespan and regulates insulin signalling in brain and fat body. [FBrf0179031] Rusten et al., 2004, Dev. Cell 7(2): 179--192 Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway. [FBrf0180118] Wessells et al., 2004, Nature Genetics 36(12): 1275--1281 Insulin regulation of heart function in aging fruit flies. [FBrf0184189] Saucedo et al., 2003, Nature Cell Biol. 5(6): 566--571 Rheb promotes cell growth as a component of the insulin/TOR signalling network. [FBrf0160913] Szuplewski et al., 2003, Development 130(16): 3651--3662 The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth. [FBrf0160992] Britton et al., 2002, Dev. Cell 2(2): 239--249 Drosophila's Insulin/PI3-Kinase pathway coordinates cellular metabolism with nutritional conditions. [FBrf0144797] Tapon et al., 2001, Cell 105(3): 345--355 The Drosophila tuberous sclerosis complex gene homologs restrict cell growth and cell proliferation. [FBrf0135684] Gao et al., 2000, Dev. Biol. 221(2): 404--418 Drosophila PTEN regulates cell growth and proliferation through PI3K-dependent and -independent pathways. [FBrf0127089]
Supplementary material	Scott et al., 2004, Dev. Cell 7: Supplementary material. [FBrf0183440]
Letter	Stocker et al., 2003, Nature Cell Biol. 5(6): 559--565 Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. [FBrf0160980]

Allele Dmel\PtenScer\UAS.cGb

Allele Dmel\Pten^Scer\UAS.cGb