FlyBase Allele Report: Dmel\Thor[2]

General Information

Symbol

Dmel\Thor²

Species

D. melanogaster

Name

FlyBase ID

FBal0117673

Feature type

allele

Associated gene

Dmel\Thor

Associated Insertion(s)

P{?'lacW}Thor²

Carried in Construct

Genomic Maps

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Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bernal et al., 2004)

Mutagen

P-element activity

(Bernal et al., 2004, Bernal and Kimbrell, 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

2L:3,478,445..3,479,963 [+]

Comment:

Approximate extent of the imprecise excision deletion of Thor. (The ambiguity in the endpoints was reported and the mapped boundaries represent the minimal extent of the deletion.)

(Bernal et al., 2004)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{lacW}Thor^k13517

(Bernal and Kimbrell, 2000)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of a P{lacW} insertion, resulting in a deletion within Thor that removes the entire coding sequence. Some sequence from the original inserted P{lacW} element remains.

(Bernal et al., 2004)

Imprecise excision of the P{lacW}Thor^k13517 insertion, deleting sequence between the original insertion site and the first BamHI site.

(Bernal and Kimbrell, 2000)

Insertion components

P{?'lacW}Thor²

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

fertile

(Tain et al., 2009, Bernal and Kimbrell, 2000)

immune response defective

(Bernal and Kimbrell, 2000)

neuroanatomy defective | adult stage

(Tain et al., 2009)

viable

(Tain et al., 2009, Bernal et al., 2004, Bernal and Kimbrell, 2000)

Phenotype Manifest In

dopaminergic PPL1 neuron

(Tain et al., 2009)

Detailed Description

Statement

Reference

30 day old Thor²/Thor² mutants show significant loss of dopaminergic PPL1 neurons compared to controls.

(Tain et al., 2009)

After 30 days of light/dark cycling, photoreceptors are preserved normally in Thor² flies.

(Wang et al., 2009)

Thor² mutant flies, when deprived of all sources of energy die faster than control flies. At 37 hours of starvation, 92% of control flies are alive, whereas only 38% of Thor² mutant flies survive. This difference is statistically significant. Thor² mutant flies may have slightly higher fat levels than in wild-type in normal conditions. Triglyceride stores drop more rapidly in Thor² mutant flies than in wild-type controls. By 24 hours of starvation, the difference in fat content is statistically significant. At 36 hours of starvation, the fat content of Thor² mutants reaches almost zero, whereas control flies still have a substantial amount of fat left at this stage. The time at which the flies have no fat left coincides with when they start dying. After 30 hours of starvation, control flies have >25% of their original fat content, whereas Thor² flies have only half as much, indicating an increased burn rate compared to wild-type. Thor² mutants do not accumulate as much excess fat as wild-type in response to the Tor-inhibitor rapamycin. In conditions of nutrient deprivation, upon addition of rapamycin, the half-life of Thor² mutants is extended by 43 hours, compared to 55 hours in wild-type. Thor² mutant flies are comparable in body weight and developmental rate to wild-type flies.

(Teleman et al., 2005)

Homozygous flies have no obvious morphological defects.

(Bernal et al., 2004)

4 days after infection with S. epidermis only 47% of mutant flies survive, compared to over 80% in wild-type. 4 days after infection with M. roseus, 16% of mutant flies survive as compared to 46% in wild-type.

(Bernal and Kimbrell, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Thor², park²⁵ has partially lethal - majority die phenotype, suppressible by Scer\GAL4^da.PU/Thor^UAS.cMa

(Tain et al., 2009)

Pink1^B9, Thor² has partially lethal - majority live phenotype, suppressible by Scer\GAL4^da.PU/Thor^UAS.cMa

(Tain et al., 2009)

Enhancer of

Statement

Reference

Thor[+]/Thor² is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Other

Statement

Reference

Thor², Trpml¹ has lethal | pupal stage phenotype

(Wong et al., 2012)

Thor², park²⁵ has partially lethal - majority die phenotype

(Tain et al., 2009)

Pink1^B9, Thor² has partially lethal - majority live phenotype

(Tain et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

Thor[+]/Thor² is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Feeding larvae high-protein yeast paste does not suppress the pupal lethality seen in Thor² trpml¹ double mutants. The addition of Rapamycin also fails to suppress the lethality.

(Wong et al., 2012)

Thor²/Thor²; park²⁵/park²⁵ double mutants are almost completely lethal and Thor²/Thor²; Pink1^B9/Pink1^B9 double mutants are partially lethal (unlike either viable single mutant or viable single mutants with the other allele heterozygotic), with rare escapers dying soon after eclosion; expression of Thor^Scer\UAS.cMa driven by Scer\GAL4^da.PU suppresses lethality in double mutants.

(Tain et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Thor² is rescued by Thor^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Teleman et al., 2005)

Comments

Overexpression of Thor^Scer\UAS.cTb, under the control of Scer\GAL4^αTub84B.PL, restores the wild-type fat burning rate in Thor² mutants, with flies retaining >25% of their original fat after 30 hours of starvation, as in wild-type.

(Teleman et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9559

y¹ w^*; Thor²

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

4E-BP

(Teleman et al., 2005)

4E-BP null

(Teleman et al., 2005)

4E-BP^null

(Kakanj et al., 2016)

Phas1²

Thor²

(Feuillette et al., 2020, Mensah et al., 2015, Pallares-Cartes et al., 2012, Wong et al., 2012, Tain et al., 2009, Teleman et al., 2005)

thor²

(Wei et al., 2019, Wang et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (12)

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