Open Close
General Information
Symbol
Dmel\Thor2
Species
D. melanogaster
Name
FlyBase ID
FBal0117673
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
Approximate extent of the imprecise excision deletion of Thor. (The ambiguity in the endpoints was reported and the mapped boundaries represent the minimal extent of the deletion.)
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of a P{lacW} insertion, resulting in a deletion within Thor that removes the entire coding sequence. Some sequence from the original inserted P{lacW} element remains.
Imprecise excision of the P{lacW}Thork13517 insertion, deleting sequence between the original insertion site and the first BamHI site.
Insertion components
P{?'lacW}Thor2
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
30 day old Thor2/Thor2 mutants show significant loss of dopaminergic PPL1 neurons compared to controls.
After 30 days of light/dark cycling, photoreceptors are preserved normally in Thor2 flies.
Thor2 mutant flies, when deprived of all sources of energy die faster than control flies. At 37 hours of starvation, 92% of control flies are alive, whereas only 38% of Thor2 mutant flies survive. This difference is statistically significant. Thor2 mutant flies may have slightly higher fat levels than in wild-type in normal conditions. Triglyceride stores drop more rapidly in Thor2 mutant flies than in wild-type controls. By 24 hours of starvation, the difference in fat content is statistically significant. At 36 hours of starvation, the fat content of Thor2 mutants reaches almost zero, whereas control flies still have a substantial amount of fat left at this stage. The time at which the flies have no fat left coincides with when they start dying. After 30 hours of starvation, control flies have >25% of their original fat content, whereas Thor2 flies have only half as much, indicating an increased burn rate compared to wild-type. Thor2 mutants do not accumulate as much excess fat as wild-type in response to the Tor-inhibitor rapamycin. In conditions of nutrient deprivation, upon addition of rapamycin, the half-life of Thor2 mutants is extended by 43 hours, compared to 55 hours in wild-type. Thor2 mutant flies are comparable in body weight and developmental rate to wild-type flies.
Homozygous flies have no obvious morphological defects.
4 days after infection with S. epidermis only 47% of mutant flies survive, compared to over 80% in wild-type. 4 days after infection with M. roseus, 16% of mutant flies survive as compared to 46% in wild-type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Feeding larvae high-protein yeast paste does not suppress the pupal lethality seen in Thor2 trpml1 double mutants. The addition of Rapamycin also fails to suppress the lethality.
Thor2/Thor2; park25/park25 double mutants are almost completely lethal and Thor2/Thor2; Pink1B9/Pink1B9 double mutants are partially lethal (unlike either viable single mutant or viable single mutants with the other allele heterozygotic), with rare escapers dying soon after eclosion; expression of ThorScer\UAS.cMa driven by Scer\GAL4da.PU suppresses lethality in double mutants.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Overexpression of ThorScer\UAS.cTb, under the control of Scer\GAL4αTub84B.PL, restores the wild-type fat burning rate in Thor2 mutants, with flies retaining >25% of their original fat after 30 hours of starvation, as in wild-type.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)