Allele Dmel\Cdk5^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Cdk5^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0117900
Feature type	allele	Associated gene	Dmel\Cdk5

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Connell-Crowley, 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of the Cdk5α cDNA tagged with T:Zzzz\FLAG. (Connell-Crowley, 2000)
Carried in construct	P{UAS-Cdk5-FLAG} (Shulman and Feany, 2003, Connell-Crowley, 2000, Connell-Crowley et al., 2000, Chatterjee et al., 2009)
Tagged with	T:Zzzz\FLAG (Connell-Crowley et al., 2000, Connell-Crowley, 2000, Shulman and Feany, 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Cdk5^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^GMR.PF has no effect on eye surface morphology. Expression of Cdk5^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^unspecified has no effect on neuron cell fate. (Connell-Crowley et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Cdk5α^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Connell-Crowley et al., 2000) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Cdk5^{Scer\UAS.T:Zzzz\FLAG}/Cdk5α^Scer\UAS.cMa is an enhancer of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
NOT Enhancer of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
NOT Suppressor of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Other
Statement Reference Cdk5alpha[+]/Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype (Shulman and Feany, 2003) Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype (Shulman and Feany, 2003) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype (Connell-Crowley et al., 2000)
Phenotype Manifest In
Enhancer of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Cdk5α^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Connell-Crowley et al., 2000) Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of presumptive embryonic/larval nervous system phenotype of Cdk5α^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Connell-Crowley et al., 2000) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Cdk5^{Scer\UAS.T:Zzzz\FLAG}/Cdk5α^Scer\UAS.cMa is an enhancer of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Cdk5^{Scer\UAS.T:Zzzz\FLAG}/Cdk5α^Scer\UAS.cMa is an enhancer of ommatidium phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
NOT Enhancer of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
NOT Suppressor of
Statement Reference Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Other
Statement Reference Cdk5alpha[+]/Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype (Shulman and Feany, 2003) Cdk5alpha[+]/Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype (Shulman and Feany, 2003) Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype (Shulman and Feany, 2003) Cdk5α^Scer\UAS.cMa, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype (Shulman and Feany, 2003) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155 has abdominal segmental nerve phenotype (Connell-Crowley et al., 2000) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155 has intersegmental nerve phenotype (Connell-Crowley et al., 2000) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype (Connell-Crowley et al., 2000) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has presumptive embryonic/larval nervous system phenotype (Connell-Crowley et al., 2000) Cdk5α^{Scer\UAS.T:Hsap\MYC}, Cdk5^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^unspecified has presumptive embryonic/larval nervous system phenotype (Connell-Crowley et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of both Cdk5^{Scer\UAS.T:Zzzz\FLAG} and Cdk5α^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF results in a mild rough eye phenotype. (Shulman and Feany, 2003) When Cdk5α^{Scer\UAS.T:Hsap\MYC} and Cdk5^{Scer\UAS.T:Zzzz\FLAG} are driven by Scer\GAL4^elav-C155, axon patterning defects are seen. SNa axons are seen lacking the dorsal branch, with ectopic muscle contacts. Overextended and misrouted TN neurons and stalled ISN neurons are also seen. When Cdk5α^{Scer\UAS.T:Hsap\MYC} and Cdk5^{Scer\UAS.T:Zzzz\FLAG} are driven by Scer\GAL4^GMR.PF, a severely disrupted eye morphology results. In embryos, the proportion seen with nervous system defects increases. (Connell-Crowley et al., 2000)
Xenogenetic Interactions
Statement Reference Co-expression of Cdk5^{Scer\UAS.T:Zzzz\FLAG} alone does not modify the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of both Cdk5^{Scer\UAS.T:Zzzz\FLAG} and Cdk5α^Scer\UAS.cMa together strongly enhances the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF. (Shulman and Feany, 2003)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Cdk5^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Chatterjee et al., 2009, Hum. Mol. Genet. 18(1): 164--177 Dissociation of tau toxicity and phosphorylation: role of GSK-3beta, MARK and Cdk5 in a Drosophila model. [FBrf0206950] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Connell-Crowley et al., 2000, Curr. Biol. 10(10): 599--602 The cyclin-dependent kinase cdk5 controls multiple aspects of axon patterning in vivo. [FBrf0128782]
Supplementary material	Connell-Crowley, 2000, Curr. Biol. 10(10): The cyclin-dependent kinase cdk5 controls multiple aspects of axon patterning in vivo. [FBrf0128834]

Allele Dmel\Cdk5Scer\UAS.T:Zzzz\FLAG

Allele Dmel\Cdk5^{Scer\UAS.T:Zzzz\FLAG}