Allele Dmel\vnc¹⁴

General Information
Symbol	Dmel\vnc¹⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0118028
Feature type	allele	Associated gene	Dmel\vnc

Allele class	hypomorphic allele - genetic evidence
Mutagen	X ray
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Rees, 1990.8.1)
Mutagen	X ray (Rees, 1990.8.1)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of a 5.2kb copia element in the predicted intron of vnc. (Wang et al., 2010)
Caused by insertion	copia{}vnc¹⁴ (Wang et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Rees, 1990.8.1) female sterile (with Df(3L)vnc^M2) (Wang et al., 2010) female sterile (with Df(3L)vnc^MK) (Wang et al., 2010) female sterile (with vnc^BDk) (Wang et al., 2010) viable (Rees, 1990.8.1)
Phenotype Manifest In
	chorion (Rees, 1990.8.1) dorsal appendage \| maternal effect (Wang et al., 2010) dorsal appendage \| maternal effect (with vnc^BDk) (Wang et al., 2010) egg \| maternal effect (Wang et al., 2010) egg \| maternal effect (with vnc^BDk) (Wang et al., 2010) egg chamber (Rees, 1990.8.1, Wang et al., 2010) egg chamber (with vnc^BDk) (Wang et al., 2010) female fusome (Wang et al., 2010) female fusome (with vnc^BDk) (Wang et al., 2010) female germline cell (Wang et al., 2010) female germline cell (with vnc^BDk) (Wang et al., 2010) nurse cell (Rees, 1990.8.1, Wang et al., 2010) nurse cell (with vnc^BDk) (Wang et al., 2010) ovary (Rees, 1990.8.1)
Detailed Description
	Statement Reference Homozygous and vnc[14]/vnc[BDk] females produce fewer eggs than wild type, the majority of which are shortened along the anterior/posterior axis and collapsed. Eggs laid by mutant females often have defects in the shape or position of the dorsal appendages (24.6% for homozygous females and 28.2% for vnc[14]/vnc[BDk] females), such as appendages that are closely apposed or fused. Many egg chambers that appear to be at stage 11 or older based on follicle cell morphology contain unusually small oocytes and persistent nurse cells, phenotypes typically resulting from a failure of nurse cells to dump their contents into the oocyte (44.4% for homozygous females and 38.5% for vnc[14]/vnc[BDk] females). 50% of homozygous egg chambers contain abnormal numbers of germline cells, showing both defects in cystocyte division (both too many and too few division) and in cyst encapsulation. 13.3% of vnc[14]/vnc[BDk] egg chambers contain abnormal numbers of germline cells showing both defects in cystocyte division and in cyst encapsulation. Fusome defects are seen in 46.7% of homozygous and 28.3% of vnc[14]/vnc[BDk] germaria. Chromatin reorganisation in the nurse cell nuclei is disrupted in mutant egg chambers, with many of the nurse cells beyond stage 5 retaining the condensed "five-blob" configuration of chromatin (70.3% of homozygous nurse cells, 72.8% of vnc[14]/vnc[BDk] nurse cells) in contrast to wild-type nurse cells at this stage (3.2%). (Wang et al., 2010) Hemizygous vnc¹⁴/Df(3L)AC1 females produce no eggs. Homozygous vnc¹⁴ females produce a few flaccid eggs with abnormal chorions (abnormal numbers of chorionic filaments and thin chorionic membranes). vnc²/vnc¹⁴ heterozygotes do produce eggs. Hemizygous vnc¹⁴/Df(3L)AC1 ovaries are small and comprised of tumorous egg chambers. Nuclei within these egg chambers appear undifferentiated. Homozygous vnc¹⁴ egg chambers range from too few through normal numbers to too many nurse cells. (Rees, 1990.8.1)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	vnc² (Rees, 1990.8.1)
Rescued by	vnc¹⁴ is rescued by vnc^+t3.1 (Wang et al., 2010) vnc^BDk/vnc¹⁴ is rescued by vnc^+t3.1 (Wang et al., 2010)
Comments
Stocks ( 1 )
Bloomington	5881 vnc¹⁴ kni^ri-1 e¹/TM3, Sb¹
Notes on Origin
Discoverer

Comments
	vnc[2] and vnc[14] contain a copia insertion in an identical location. (Wang et al., 2010) vnc¹⁴ slightly weaker phenotype than vnc². (Rees, 1990.8.1)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	vnc¹⁴ (Wang et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wang et al., 2010, Dev. Dyn. 239(11): 2813--2827 Drosophila variable nurse cells encodes arrest defective 1 (ARD1), the catalytic subunit of the major N-terminal acetyltransferase complex. [FBrf0212164]
Personal communication to FlyBase	Rees, 1990.8.1, vnc alleles. vnc alleles. [FBrf0131220]

Allele Dmel\vnc14

Allele Dmel\vnc¹⁴