Allele Dmel\mei-P26^fs1

General Information
Symbol	Dmel\mei-P26^fs1	Species	D. melanogaster
Name		FlyBase ID	FBal0118093
Feature type	allele	Associated gene	Dmel\mei-P26

Allele class	hypomorphic allele - genetic evidence
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Glasscock et al., 2005)
Mutagen	Delta2-3 (Page et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	mei-P26¹ (Page et al., 2000)
Nature of the lesion	Statement Reference Tandem duplication of the P{lacW} element, such that two copies of the P{lacW} element are present in the first intron. (Page et al., 2000)
Caused by insertion	P{lacW}mei-P26¹ (Page et al., 2000) P{lacW}mei-P26^fs1 (Page et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| recessive (Page et al., 2000) neurophysiology defective (with mei-P26¹⁶) (Glasscock et al., 2005)
Phenotype Manifest In
	egg (Page et al., 2000) egg chamber (Page et al., 2000) egg chamber (with mei-P26¹) (Liu et al., 2009) egg chamber (with mei-P26^mfs1) (Liu et al., 2009) nucleolus & ovary (Neumuller et al., 2008) nurse cell (Page et al., 2000) nurse cell (with mei-P26¹) (Liu et al., 2009) ovary (Starr and Cline, 2002)
Detailed Description
	Statement Reference mei-P26[1]/mei-P26[fs1] ovaries are mostly wild-type in appearance, with less than 5% of egg chambers having fewer or more than 15 nurse cells or being incorrectly patterned. (Liu et al., 2009) Nucleoli in mutant ovaries are larger than those in wild-type ovaries. Cell diameters are increased compared to wild type and no longer decrease as cells are displaced from the stem cell niche in mei-P26[fs1]/mei-P26[mfs1] ovaries. (Neumuller et al., 2008) mei-P26¹⁶/mei-P26^fs1 flies have a high seizure threshold (the minimum voltage required to induce seizure activity in the dorsal longitudinal muscles after a high-frequency stimulus) compared to controls. mei-P26¹⁶/mei-P26^fs1 flies show defects in X chromosome nondisjunction. (Glasscock et al., 2005) The ovary phenotypes of homozygous females are not suppressed by Wolbachia infection; 100% of ovaries of homozygous infected females have no eggs. (Starr and Cline, 2002) mei-P26¹/mei-P26^fs1 females show 22.4% X chromosome nondisjunction and 18.1% 4th chromosome nondisjunction. Recombination frequency on chromosome 2 is decreased in mei-P26¹/mei-P26^fs1 females and the recombination distribution is polar. Homozygous ovaries often contain chamber cysts filled with large numbers of small, apparently undifferentiated cells. 84.4% of egg chambers show this "tumourous" phenotype. The remaining chambers more closely resemble wild type, in that nurse cells are present, although many contain abnormal numbers of nurse cells. Homozygous females lay a reduced number of eggs compared to wild type. (Page et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference mei-P26^fs1/mei-P26¹⁶ is a suppressor \| partially of bang sensitive phenotype of eas² (Glasscock et al., 2005) mei-P26^fs1/mei-P26¹⁶ is a suppressor \| partially of bang sensitive phenotype of sda^iso7.8 (Glasscock et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference mei-P26^fs1/mei-P26¹ has egg chamber phenotype, enhanceable by vas^PH165/vas¹ (Liu et al., 2009) mei-P26^fs1/mei-P26¹ has nurse cell phenotype, enhanceable by vas^PH165/vas¹ (Liu et al., 2009) mei-P26^fs1/mei-P26¹ has oocyte phenotype, enhanceable by vas^PH165/vas¹ (Liu et al., 2009)
Suppressed by
Statement Reference mei-P26^fs1 has ovary phenotype, suppressible \| partially by loqs[+]/loqs^KO (Neumuller et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference More than 50% of vas[1]/vas[PH165] ; mei-P26[1]/mei-P26[fs1] egg chambers are tumorous and fail to differentiate nurse cells and oocytes. The double mutant egg chambers contain mostly early-stage cystocytes as they contain either punctate spectrosomes or branched fusomes. (Liu et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	mei-P26¹ (Page et al., 2000) mei-P26¹⁶ (Glasscock et al., 2005) mei-P26^fs2 (Page et al., 2000) mei-P26^fs3 (Page et al., 2000) mei-P26^fs4 (Page et al., 2000) mei-P26^fs5 (Page et al., 2000) mei-P26^fs6 (Page et al., 2000)
Rescued by	mei-P26^fs1/mei-P26¹ is rescued by mei-P26^+t13 (Page et al., 2000) mei-P26^fs1 is rescued by mei-P26^+t13 (Page et al., 2000)
Comments	Reduced fertility in combination with mei-P26¹, fails to complement the mei-P26¹ nondisjunction phenotype. (Page et al., 2000)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mei-P26^fs1 (Neumuller et al., 2008, Liu et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Liu et al., 2009, Genes Dev. 23(23): 2742--2752 Vasa promotes Drosophila germline stem cell differentiation by activating mei-P26 translation by directly interacting with a (U)-rich motif in its 3' UTR. [FBrf0209427] Neumuller et al., 2008, Nature 454(7201): 241--245 Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage. [FBrf0206272] Glasscock et al., 2005, Genetics 170(4): 1677--1689 The mei-P26 Gene Encodes a RING Finger B-box Coiled-Coil-NHL Protein That Regulates Seizure Susceptibility in Drosophilia. [FBrf0187640] Starr and Cline, 2002, Nature 418(6893): 76--79 A host parasite interaction rescues Drosophila oogenesis defects. [FBrf0151438] Page et al., 2000, Genetics 155(4): 1757--1772 Genetic studies of mei-P26 reveal a link between the processes that control germ cell proliferation in both sexes and those that control meiotic exchange in Drosophila. [FBrf0130005]

Allele Dmel\mei-P26fs1

Allele Dmel\mei-P26^fs1