FlyBase Allele Report: Dmel\hiw[ND8]

General Information

Symbol

Dmel\hiw^ND8

Species

D. melanogaster

Name

FlyBase ID

FBal0118196

Feature type

allele

Associated gene

Dmel\hiw

Associated Insertion(s)

Carried in Construct

Also Known As

ND8

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Wan et al., 2000)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: Y1930term.

(Wan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:15,054,603..15,054,603 [-]

Nucleotide change:

G15054603A

Amino acid change:

W1930term | hiw-PA; W1930term | hiw-PB

Reported amino acid change:

Y1930term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Wan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tuberous sclerosis

modeled by Tsc1^UAS.cTa, gig^UAS.cTa

(Murthy et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Uthaman et al., 2008, Wu et al., 2005)

abnormal neuroanatomy | larval stage

(Klinedinst et al., 2013)

abnormal neuroanatomy | third instar larval stage

(Russo and DiAntonio, 2019, Wong et al., 2015)

abnormal neurophysiology

(Uthaman et al., 2008, Collins et al., 2006)

abnormal size | third instar larval stage

(Russo and DiAntonio, 2019)

viable

(Wan et al., 2000)

Phenotype Manifest In

bouton

(Collins et al., 2006)

embryonic/larval neuromuscular junction, with Scer\GAL4^{elav.Switch.PO}, hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}

(Tian et al., 2011)

embryonic/larval neuromuscular junction, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔN.UAS.Tag:TAP}

(Tian et al., 2011)

embryonic/larval neuromuscular junction, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔPHR.UAS.Tag:TAP}

(Tian et al., 2011)

embryonic/larval neuromuscular junction, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔRCC1.UAS.Tag:TAP}

(Tian et al., 2011)

embryonic/larval neuromuscular junction (with hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

embryonic/larval neuromuscular junction (with hiw^{ΔN.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

embryonic/larval neuromuscular junction (with hiw^{ΔPHR.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

embryonic/larval neuromuscular junction (with hiw^{ΔRCC1.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

embryonic/larval neuromuscular junction | third instar larval stage

(Russo and DiAntonio, 2019, Wong et al., 2015)

giant fiber neuron

(Uthaman et al., 2008)

indirect flight muscle motor neuron DLMN4

(Wu et al., 2005)

mesothoracic tergotrochanter muscle motor neuron

(Uthaman et al., 2008)

motor neuron (with Df(1)RK4)

(Wan et al., 2000)

neuromuscular junction

(Collins et al., 2006, Wu et al., 2005)

neuromuscular junction | larval stage

(Klinedinst et al., 2013)

NMJ bouton, with Scer\GAL4^{elav.Switch.PO}, hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}

(Tian et al., 2011)

NMJ bouton, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔN.UAS.Tag:TAP}

(Tian et al., 2011)

NMJ bouton, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔPHR.UAS.Tag:TAP}

(Tian et al., 2011)

NMJ bouton, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔRCC1.UAS.Tag:TAP}

(Tian et al., 2011)

NMJ bouton (with hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

NMJ bouton (with hiw^{ΔN.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

NMJ bouton (with hiw^{ΔPHR.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

NMJ bouton (with hiw^{ΔRCC1.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

NMJ bouton | increased number | third instar larval stage

(Russo and DiAntonio, 2019)

NMJ bouton | larval stage

(Klinedinst et al., 2013)

NMJ bouton | third instar larval stage

(Wong et al., 2015)

synapse

(Wu et al., 2005)

synapse, with Scer\GAL4^{elav.Switch.PO}, hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}

(Tian et al., 2011)

synapse, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔN.UAS.Tag:TAP}

(Tian et al., 2011)

synapse, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔPHR.UAS.Tag:TAP}

(Tian et al., 2011)

synapse, with Scer\GAL4^{elav.Switch.PO}, hiw^{ΔRCC1.UAS.Tag:TAP}

(Tian et al., 2011)

synapse (with hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

synapse (with hiw^{ΔN.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

synapse (with hiw^{ΔPHR.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

synapse (with hiw^{ΔRCC1.UAS.Tag:TAP}), with Scer\GAL4^{elav.Switch.PO}

(Tian et al., 2011)

terminal bouton

(Wu et al., 2005)

Detailed Description

Statement

Reference

hiw^ND8 third instar larvae exhibit dramatic overgrowth of the neuromuscular junction, namely increased branching and increased number of presynaptic boutons, as compared to controls.

(Russo and DiAntonio, 2019)

Mutant larvae show an increase in both branch and bouton number at the neuromuscular junction compared to controls.

(Klinedinst et al., 2013)

Expression of hiw^{ΔPHR.Scer\UAS.T:Zzzz\TAP} presynaptically in hiw^ND8 heterozygotes, under the control of Scer\GAL4^{elav.Switch.PO} results in mild synaptic terminal overgrowth.

Expression of hiw^{ΔRCC1.Scer\UAS.T:Zzzz\TAP} presynaptically in hiw^ND8 heterozygotes, under the control of Scer\GAL4^{elav.Switch.PO} results in moderate synaptic terminal overgrowth.

Expression of hiw^{HindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} presynaptically in hiw^ND8 heterozygotes, under the control of Scer\GAL4^{elav.Switch.PO} results in moderate synaptic terminal overgrowth.

Expression of hiw^{ΔN.Scer\UAS.T:Zzzz\TAP} presynaptically in hiw^ND8 heterozygotes, under the control of Scer\GAL4^{elav.Switch.PO} results in notable synaptic terminal overgrowth.

(Tian et al., 2011)

hiw^ND8 mutants exhibit defects in the giant fiber-tergotrochanteral motorneuron synapse, such as ectopic axonal branches from the presynaptic terminal.

(Uthaman et al., 2008)

hiw^ND8 mutants exhibit dramatic synaptic overgrowth at the neuromuscular junction of muscle 4: a 4-fold increase in the number of boutons and synaptic branches, and a 2-fold increase in total synaptic area. The hiw^ND8 mutant phenotype also causes a 66% reduction in bouton size and a 70% reduction in the average intensity and a 50% reduction in total intensity of staining for synaptic vesicle proteins.

hiw^ND8 mutants show both reduced quantal size (response to a single vesicle) and reduced quantal content (number of vesicles released by the nerve).

(Collins et al., 2006)

Morphological analysis at the neuromuscular junction of hiw^ND8 mutants reveals a dramatic synaptic overgrowth phenotype apparent at every type I synapse.

Using the MN4-Ib motoneuron as a model, hiw^ND8 mutants exhibit a fivefold increase in the numbers of both synaptic bouton and branch points when compared with wild-type neuromuscular junctions. The synaptic span also increases from approximately 15% in wild-type to approximately 40%. Although there is an increase in the number of synaptic boutons, there is an approximate 70% decrease in the average bouton size compared to wild-type.

(Wu et al., 2005)

Eye size is similar to controls in mutant flies.

(Murthy et al., 2004)

The pattern of motor innervation of muscles 12 and 13 is also correct in hiw^unspecified mutants, although the terminal branches are much longer and more numerous.

(Wan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wnd^GL00282/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has abnormal size | third instar larval stage phenotype, suppressible by wnd^GL00282/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has abnormal size | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Trpml¹

(Wong et al., 2015)

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/RagA-B^T16N.UAS

(Wong et al., 2015)

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/raptor^JF01088

(Wong et al., 2015)

hiw^ND8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cln3^HMS01476/Scer\GAL4^VGlut1-OK371

(Wong et al., 2015)

hiw^ND8 has abnormal neuroanatomy | larval stage phenotype, suppressible by p38b^KG01337/Df(2L)b80e3

(Klinedinst et al., 2013)

hiw^ND8 has abnormal neuroanatomy | larval stage phenotype, suppressible by p38a¹

(Klinedinst et al., 2013)

hiw^ND8 has abnormal neurophysiology phenotype, suppressible by wnd¹/wnd²

(Collins et al., 2006)

NOT suppressed by

Statement

Reference

hiw^ND8 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^BG380/Jra^Jbz.UAS

(Collins et al., 2006)

NOT Enhancer of

Statement

Reference

hiw^ND8 is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Suppressor of

Statement

Reference

hiw^ND8 is a suppressor of abnormal neuroanatomy | larval stage phenotype of p38b^KG01337/Df(2L)b80e3

(Klinedinst et al., 2013)

NOT Suppressor of

Statement

Reference

hiw^ND8 is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

hiw^ND8 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by wnd^GL00282/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by wnd^GL00282/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Trpml¹

(Wong et al., 2015)

hiw^ND8 has NMJ bouton | third instar larval stage phenotype, suppressible by Trpml¹

(Wong et al., 2015)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/RagA-B^T16N.UAS

(Wong et al., 2015)

hiw^ND8 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/RagA-B^T16N.UAS

(Wong et al., 2015)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/raptor^JF01088

(Wong et al., 2015)

hiw^ND8 has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^VGlut1-OK371/raptor^JF01088

(Wong et al., 2015)

hiw^ND8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Cln3^HMS01476/Scer\GAL4^VGlut1-OK371

(Wong et al., 2015)

hiw^ND8 has NMJ bouton | third instar larval stage phenotype, suppressible by Cln3^HMS01476/Scer\GAL4^VGlut1-OK371

(Wong et al., 2015)

hiw^ND8 has NMJ bouton | larval stage phenotype, suppressible by p38b^KG01337/Df(2L)b80e3

(Klinedinst et al., 2013)

hiw^ND8 has neuromuscular junction | larval stage phenotype, suppressible by p38b^KG01337/Df(2L)b80e3

(Klinedinst et al., 2013)

hiw^ND8 has NMJ bouton | larval stage phenotype, suppressible by p38a¹

(Klinedinst et al., 2013)

hiw^ND8 has neuromuscular junction | larval stage phenotype, suppressible by p38a¹

(Klinedinst et al., 2013)

hiw^ND8 has bouton phenotype, suppressible by Scer\GAL4^BG380/bsk^K53R.UAS

(Collins et al., 2006)

hiw^ND8 has neuromuscular junction phenotype, suppressible by wnd[+]/wnd¹

(Collins et al., 2006)

hiw^ND8 has bouton phenotype, suppressible by wnd[+]/wnd¹

(Collins et al., 2006)

hiw^ND8 has neuromuscular junction phenotype, suppressible by wnd¹/wnd²

(Collins et al., 2006)

hiw^ND8 has bouton phenotype, suppressible by wnd¹/wnd²

(Collins et al., 2006)

hiw^ND8 has neuromuscular junction phenotype, suppressible by Scer\GAL4^BG380/bsk^K53R.UAS

(Collins et al., 2006)

NOT suppressed by

Statement

Reference

hiw^ND8 has neuromuscular junction phenotype, non-suppressible by p38a¹/Scer\GAL4^BG380/p38b^DN.UAS

(Collins et al., 2006)

hiw^ND8 has bouton phenotype, non-suppressible by p38a¹/Scer\GAL4^BG380/p38b^DN.UAS

(Collins et al., 2006)

hiw^ND8 has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^BG380/Jra^Jbz.UAS

(Collins et al., 2006)

hiw^ND8 has bouton phenotype, non-suppressible by Scer\GAL4^BG380/Jra^Jbz.UAS

(Collins et al., 2006)

NOT Enhancer of

Statement

Reference

hiw^ND8 is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Suppressor of

Statement

Reference

hiw^ND8 is a suppressor of NMJ bouton | larval stage phenotype of p38b^KG01337/Df(2L)b80e3

(Klinedinst et al., 2013)

NOT Suppressor of

Statement

Reference

hiw^ND8 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

hiw^ND8;Trpml¹ double mutant larvae do not show the increase in number of neuromuscular junction boutons characteristic for the hiw^ND8 single mutants.

Ectopic expression of RagA-B^{T16N.Scer\UAS} under the control of the Scer\GAL4^VGlut-OK371 driver in hiw^ND8 mutant larvae background rescues the increased number of neuromuscular junction boutons phenotype characteristic for hiw^ND8 mutants.

Ectopic expression of raptor^JF01088 under the control of the Scer\GAL4^VGlut-OK371 driver in hiw^ND8 mutant larvae background partially rescues the increased number of neuromuscular junction boutons phenotype characteristic for hiw^ND8 mutants.

Expression of cln3^HMS01476 RNAi under the control of the Scer\GAL4^VGlut-OK371 in the hiw^ND8 mutant background lowers the increased number of neuromuscular junction boutons characteristic for the hiw^ND8 mutant third instar larvae.

(Wong et al., 2015)

p38b^KG01337/Df(2L)b80e3 suppresses the increase in branch and bouton number at the neuromuscular junction which is seen in hiw^ND8 larvae. hiw^ND8 suppresses the increase in maximum bouton diameter which is seen at the neuromuscular junction in p38b^KG01337/Df(2L)b80e3 larvae.

Mpk2¹ suppresses the increase in branch and bouton number at the neuromuscular junction which is seen in hiw^ND8 larvae.

(Klinedinst et al., 2013)

Simultaneous expression of p38b^DN.Scer\UAS (under the control of Scer\GAL4^BG380) and Mpk2¹ does not suppress the hiw^ND8 synaptic phenotype.

Expression of bsk^{K53R.Scer\UAS} (under the control of Scer\GAL4^BG380) suppresses the hiw^ND8 synaptic phenotype, reducing the number of synaptic boutons and branches and increasing bouton size and the intensity of synaptic vesicle markers.

Removing one copy of wnd¹ suppresses the hiw^ND8 neuromuscular junction of muscle 4 phenotype by approximately 50%.

hiw^ND8; wnd¹/wnd² mutants are not significantly different from wild-type, indicating complete suppression of hiw^ND8.

wnd¹/wnd² mutants do not suppress the hiw^ND8 defect in quantal size: both the amplitude and the frequency of spontaneous miniature events in the hiw^ND8; wnd¹/wnd² double mutant are similar to wild-type. In contrast, the evoked potentials of the double mutant are only modestly increased, and this is due entirely to the increased quantal size. The quantal content, calculated as the excitatory junction potential (EJP) amplitude divided by the miniature EJP (mEJP) remains the same between hiw^ND8 and hiw^ND8; wnd¹/wnd² double mutants. Therefore wnd does not suppress the primary defect in hiw^ND8 synaptic function, the reduced number of vesicles released by the nerve.

Expression of kay^Fbz.Scer\UAS, under the control of Scer\GAL4^BG380, confers dramatic suppression of the hiw^ND8 synaptic phenotype, reducing bouton number and branching (42%) and increasing the intensity of staining for synaptic vesicle markers at the synapse.

Expression of Jra^Jbz.Scer\UAS, under the control of Scer\GAL4^BG380 does not suppress the hiw^ND8 synaptic phenotype.

(Collins et al., 2006)

The small eye phenotype caused by co-expression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cTa under the control of Scer\GAL4^ey.PH is enhanced by hiw^ND8.

(Murthy et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hiw^ND8 is rescued by Scer\GAL4^elav-C155/hiw^UAS.Tag:TAP

(Tian et al., 2011)

hiw^ND8 is rescued by hiw^UAS.fl/Scer\GAL4^elav.PLu

(Wu et al., 2005)

hiw^ND8 is rescued by Scer\GAL4^{elav.Switch.PO}/hiw^UAS.W.EGFP

(Wu et al., 2005)

Not rescued by

hiw^ND8 is not rescued by hiw^{ΔRING.UAS.Tag:TAP}/Scer\GAL4^elav-C155

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{ΔRCC1.UAS.Tag:TAP}

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{ΔPHR.UAS.Tag:TAP}

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{HindIII.UAS.Tag:polyHis,Tag:MYC}

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{ΔC.UAS.Tag:TAP}

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{ΔN.UAS.Tag:TAP}

(Tian et al., 2011)

hiw^ND8 is not rescued by Scer\GAL4^elav-C155/hiw^{CT1000.UAS.Tag:TAP}

(Tian et al., 2011)

hiw^ND8 is not rescued by hiw^UAS.fl/Scer\GAL4^elav.PLu

(Wu et al., 2005)

hiw^ND8 is not rescued by hiw^ΔRING.UAS/Scer\GAL4^elav.PLu

(Wu et al., 2005)

hiw^ND8 is not rescued by hiw^{ΔRING.UAS.Tag:TAP}/Scer\GAL4^elav.PLu

(Wu et al., 2005)

Comments

Presynaptic expression of hiw^Scer\UAS.fl (under the control of Scer\GAL4^elav.PLu) resuces the deficit in both spontaneous and evoked neurotransmitter release in hiw^ND8 hemizygotes. The quantal content, which is estimated by the mean mEJP amplitude, is also rescued.

Expresion of hiw^Scer\UAS.fl postsynaptically, under the control of Scer\GAL4^G7 fails to rescue the hiw^ND8 synaptic overgrowth phenotype.

Expression of hiw^{Scer\UAS.T:Avic\GFP-EGFP} throughout development, under the control of Scer\GAL4^{elav.Switch.PO}, results in a robust rescue of the hiw^ND8/Y morphological phenotype with an approximate 77% reduction in the number of boutons, along with robust rescue of quantal content.

In the absence of RU486, which activates transcription of Scer\GAL4^{elav.Switch.PO}, and therefore hiw^{Scer\UAS.T:Avic\GFP-EGFP}, hiw^ND8/Y synapses are still extremely overgrown; however, there is apparently some leaky expression because there is a 27% decrease in the number of synaptic boutons. Leaky expression leads to a nearly complete rescue of the quantal content, indicating that very low dose of hiw are effective in rescuing synaptic function.

When RU486 is added during larval development, there is an additional approximately 25% reduction in bouton number, compared with hiw^ND8/Y larvae in the absence of RU486. Compared with leaky expression, late expression of hiw^{Scer\UAS.T:Avic\GFP-EGFP} does not lead to a significant decrease in synaptic branching or synaptic expansion in hiw^ND8/Y mutants. There is a complete rescue of synaptic function, with a significant increase in quantal content compared to leaky hiw^{Scer\UAS.T:Avic\GFP-EGFP} expression in the absence of RU486.

(Wu et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51638

w^* hiw^ND8

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ND8

(Wu et al., 2005, Murthy et al., 2004)

hiw^ND8

(Hao et al., 2019, Russo and DiAntonio, 2019, Sulkowski et al., 2016, Wong et al., 2015, Klinedinst et al., 2013, Shin and Diantonio, 2011, Tian et al., 2011, Xiong et al., 2010, Zhao et al., 2009, Uthaman et al., 2008, Collins et al., 2006)

hiw^nd8

(Mozer and Sandstrom, 2012)

Name Synonyms

Secondary FlyBase IDs

References (16)

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