FlyBase Allele Report: Dmel\futsch[K68]

General Information

Symbol

Dmel\futsch^K68

Species

D. melanogaster

Name

FlyBase ID

FBal0118232

Feature type

allele

Associated gene

Dmel\futsch

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Franco et al., 2004, Hummel et al., 2000)

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Troyer syndrome

modeled by spartin¹

(Nahm et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

futsch^K68 larval class IV da neurons show a significant increase in the number of dendrite tips, as compared to controls.

(Jenkins et al., 2017)

futsch^K68/futsch^K68 mutant larval neuromuscular junctions do not exhibit aberrant microtubule accumulation; but do show a small but significant decrease in the diameter of the microtubule core filament; mutants also show a significant reduction in synaptic bouton number; and there is a non-significant reduction in evoked postsynaptic potential (EPSP) or mEPSP amplitude and quantal content, as compared to controls. Type 1b axons innervating NMJs do not exhibit microtubule organization defects in futsch^K68/futsch^K68 mutant larvae.

(Stephan et al., 2015)

20 day old adult heterozygotes show increased vacuolisation in the brain compared to controls.

(Nahm et al., 2013)

Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.

(Senthilan et al., 2012)

There is a decrease in the number of synaptic boutons at the neuromuscular junction in mutant third instar larvae.

(Gogel et al., 2006)

futsch^K68 mutants display a 20% decrease in the number of boutons in larval neuromuscular junctions and a decrease in the number of "satellite" boutons, defined as boutons that are present in the axon of a branch or that bud off from neuronal connections between two boutons.

(Franco et al., 2004)

Mutant animals exhibit increased microtubule fragmentation in neuromuscular junction synaptic boutons.

(Ruiz-Canada et al., 2004)

Neuronal development in the embryo proceeds normally. In larvae a reduced number of synaptic boutons are seen.

(Hummel et al., 2000)

In futsch^K68 homozygotes, synaptic microtubules no longer form a filamentous cytoskeletal shaft that runs through the nerve terminal, and microtubule loop formation is absent. There is also a reduction in bouton number and an increase in bouton size (bouton sizes increase over two-fold). Bouton number is reduced from an average of 64.8 boutons at muscle 4 in wild-type to 37.9 in mutants. Also mutant nerve-terminals at muscle 4 rarely have branch-points. No change in muscle size is seen in these mutants.

(Roos et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Ank2^XLΔ/Ank2^XLΔ

(Stephan et al., 2015)

Suppressed by

Statement

Reference

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2^XL-5xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2^XL-20xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has abnormal neuroanatomy | dominant | adult stage phenotype, suppressible by spartin¹/Df(3R)110

(Nahm et al., 2013)

NOT suppressed by

Statement

Reference

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Ank2^XL-Δall, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

Suppressor of

Statement

Reference

futsch^K68/futsch^K68 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Ank2^XLΔ

(Stephan et al., 2015)

futsch^K68/futsch[+] is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

NOT Suppressor of

Statement

Reference

futsch^K68/futsch[+] is a non-suppressor of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

futsch^K68/futsch^K68 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Ank2^f00518

(Stephan et al., 2015)

futsch^K68/futsch^K68 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Df(2R)Exel9026, P{Flo1^wt.Cdk5^Δ.CG30466^wt.CG8204^wt}

(Kissler et al., 2009)

Other

Statement

Reference

Ank2^XLΔ, futsch^K68 has abnormal neurophysiology phenotype

(Stephan et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by Ank2^XLΔ/Ank2^XLΔ

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, enhanceable by Ank2^XLΔ/Ank2^XLΔ

(Stephan et al., 2015)

Suppressed by

Statement

Reference

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, suppressible | partially by Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2^XL-5xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, suppressible | partially by Ank2^XL-5xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2^XL-20xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, suppressible | partially by Ank2^XL-20xR, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has adult brain phenotype, suppressible by spartin¹/Df(3R)110

(Nahm et al., 2013)

NOT suppressed by

Statement

Reference

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Ank2^XL-Δall, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, non-suppressible by Ank2^XL-Δall, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has microtubule | larval stage phenotype, non-suppressible by Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518

(Stephan et al., 2015)

futsch^K68 has bouton phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/sgg^A81T.UAS

(Franco et al., 2004)

futsch^K68 has bouton phenotype, non-suppressible by Scer\GAL4^futsch-C380/aPKC^M.UAS

(Ruiz-Canada et al., 2004)

Suppressor of

Statement

Reference

futsch^K68/futsch^K68 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2^XLΔ

(Stephan et al., 2015)

futsch^K68/futsch^K68 is a suppressor | partially of microtubule | larval stage phenotype of Ank2^XLΔ

(Stephan et al., 2015)

futsch^K68/futsch[+] is a suppressor of adult brain | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

futsch^K68/futsch[+] is a suppressor of type I bouton | increased number phenotype of foxo²¹

(Nechipurenko and Broihier, 2012)

futsch^K68/futsch[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of foxo²¹

(Nechipurenko and Broihier, 2012)

futsch^K68/futsch[+] is a suppressor of synaptic vesicle phenotype of foxo²¹

(Nechipurenko and Broihier, 2012)

futsch^K68/futsch[+] is a suppressor of microtubule phenotype of foxo²¹

(Nechipurenko and Broihier, 2012)

futsch^K68 is a suppressor of bouton | increased number phenotype of Scer\GAL4^RapGAP1-OK6, sgg^A81T.UAS

(Franco et al., 2004)

NOT Suppressor of

Statement

Reference

futsch^K68/futsch[+] is a non-suppressor of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

futsch^K68/futsch[+] is a non-suppressor of dendrite | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

futsch^K68/futsch^K68 is a non-suppressor of neuromuscular junction | larval stage phenotype of Ank2^f00518

(Stephan et al., 2015)

futsch^K68/futsch^K68 is a non-suppressor of synapse | larval stage phenotype of Ank2^f00518

(Stephan et al., 2015)

futsch^K68/futsch^K68 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Df(2R)Exel9026, P{Flo1^wt.Cdk5^Δ.CG30466^wt.CG8204^wt}

(Kissler et al., 2009)

Other

Statement

Reference

Ank2^XLΔ, futsch^K68 has synapse | larval stage phenotype

(Stephan et al., 2015)

Ank2^XLΔ, futsch^K68 has NMJ bouton | larval stage phenotype

(Stephan et al., 2015)

Ank2^XLΔ, futsch^K68 has motor neuron | larval stage phenotype

(Stephan et al., 2015)

Ank2^XLΔ, futsch^K68 has axon | larval stage phenotype

(Stephan et al., 2015)

wrd¹⁰⁴, futsch^K68/futsch[+] has bouton & neuromuscular junction | larval stage phenotype

(Viquez et al., 2006)

PP2A-B'[+]/wrd¹⁰⁴, futsch^K68 has bouton & neuromuscular junction | larval stage phenotype

(Viquez et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1^HMS00405 under the control of Scer\GAL4^ppk.PG is not significantly modified by combination with a single copy of futsch^K68.

(Herzmann et al., 2017)

futsch^K68/futsch^K68 suppresses the aberrant microtubule aggregations in Ank2^XLΔ/Ank2^XLΔ mutant larval neuromuscular junctions and the axons innervating NMJs, but Ank2^XLΔ/Ank2^XLΔ enhances the decrease in the diameter of the microtubule core filament seen in futsch^K68/futsch^K68 mutants; futsch^K68/futsch^K68, Ank2^XLΔ/Ank2^XLΔ double mutants show perturbation of the area of individual synaptic boutons, either dramatically increased or reduced to small varicosities; double mutants exhibit a dramatic reduction in evoked postsynaptic potential (EPSP) amplitude, quantal content, and propagation time from stimulation artefact to the start of EPSP, but no changes in mEPSP amplitude; axon diameter of individual muscle 4 motoneurons is significantly reduced; and double mutants exhibit a significant increase in the fraction of stationary mitochondria and a decrease in anterograde transport velocities in axons, as compared to controls.

Expression of Ank2^XL-ΔR, Ank2^XL-5xR or Ank2^XL-20xR, but not Ank2^XL-Δall or Ank2^XL-ΔC, in a Ank2^f00518/Ank2^f00518 mutant background partially rescues the decreased microtubule core filament area seen in futsch^K68/futsch^K68 mutant larval neuromuscular junctions.

futsch^K68/futsch^K68 fails to suppress the defective synaptic stability of Ank2^f00518/Ank2^f00518 mutant larval neuromuscular junctions.

(Stephan et al., 2015)

The brain vacuolisation seen in 20 day old spartin¹/Df(3R)110 and futsch^K68/+ adults is suppressed in futsch^K68/+ ; spartin¹/Df(3R)110 double mutant flies.

(Nahm et al., 2013)

A futsch^K68/+ background suppresses the increase in type 1b bouton area observed in foxo²¹ homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo²¹ mutants.

A futsch^K68/+ background suppresses the FM 1-43 loading defects found in foxo²¹ mutant synapses.

(Nechipurenko and Broihier, 2012)

The synaptic overgrowth phenotype at the neuromuscular junction seen in third instar larvae lacking Cdk5 function (Df(2R)Exel9026 homozygotes with all deleted gene functions except Cdk5 restored using P{Flo-1^wt.Cdk5^Δ.CG30466^wt.CG8204^wt}) is not altered if the larvae are also homozygous or futsch^K68.

(Kissler et al., 2009)

The neuromuscular junction of futsch^K68/+; PP2A-B'¹⁰⁴/+ larvae have significantly larger boutons than wild-type. In contrast, either heterozygote alone has normal sized boutons.

(Viquez et al., 2006)

Expression of sgg^{A81T.Scer\UAS} under the control of Scer\GAL4^OK6, in a futsch^K68 background shows the futsch^K68 phenotype in which larval neuromuscular synaptic bouton number is decreased and not the opposite sgg^{A81T.Scer\UAS} overexpression phenotype.

(Franco et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

futsch^K68 is partially rescued by futsch^N-Term.UAS/futsch^C-Term.UAS/Scer\GAL4^da.G32

(Gogel et al., 2006)

futsch^K68 is partially rescued by Scer\GAL4^da.G32/futsch^N+C-Term.UAS

(Gogel et al., 2006)

futsch^K68 is partially rescued by futsch^NPC-Term.UAS/Scer\GAL4^da.G32

(Gogel et al., 2006)

futsch^K68 is partially rescued by Scer\GAL4^elav-C155/futsch^Nterm.UAS

(Roos et al., 2000)

Not rescued by

futsch^K68 is not rescued by futsch^N-Term.UAS/Scer\GAL4^da.G32

(Gogel et al., 2006)

futsch^K68 is not rescued by futsch^C-Term.UAS/Scer\GAL4^da.G32

(Gogel et al., 2006)

Comments

Expression of futsch^{N-Term.Scer\UAS} or futsch^{C-Term.Scer\UAS} separately under the control of Scer\GAL4^da.G32 fails to rescue the reduction in bouton number at the neuromuscular junction seen in futsch^K68 third instar larvae.

Expression of futsch^{N-Term.Scer\UAS} and futsch^{C-Term.Scer\UAS} together under the control of Scer\GAL4^da.G32 partially the reduction in bouton number at the neuromuscular junction seen in futsch^K68 third instar larvae.

Expression of either futsch^{N+C-Term.Scer\UAS} or futsch^{NPC-Term.Scer\UAS} separately under the control of Scer\GAL4^da.G32 partially rescues the reduction in bouton number at the neuromuscular junction seen in futsch^K68 third instar larvae.

(Gogel et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

futsch^K68

(Vargas et al., 2020, Migh et al., 2018, Herzmann et al., 2017, Jenkins et al., 2017, Yalgin et al., 2015, Nahm et al., 2013, Nechipurenko and Broihier, 2012, Senthilan et al., 2012, Lee et al., 2010, Kissler et al., 2009, Gogel et al., 2006, Franco et al., 2004)

futsch^k68

(Sears and Broihier, 2016, Stephan et al., 2015, Ruiz-Canada et al., 2004)

futsch^kx68

(Zou et al., 2008)

futschK68

(Viquez et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (19)

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