synapse & microtubule
futschK68 larval class IV da neurons show a significant increase in the number of dendrite tips, as compared to controls.
futschK68/futschK68 mutant larval neuromuscular junctions do not exhibit aberrant microtubule accumulation; but do show a small but significant decrease in the diameter of the microtubule core filament; mutants also show a significant reduction in synaptic bouton number; and there is a non-significant reduction in evoked postsynaptic potential (EPSP) or mEPSP amplitude and quantal content, as compared to controls. Type 1b axons innervating NMJs do not exhibit microtubule organization defects in futschK68/futschK68 mutant larvae.
20 day old adult heterozygotes show increased vacuolisation in the brain compared to controls.
Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.
There is a decrease in the number of synaptic boutons at the neuromuscular junction in mutant third instar larvae.
futschK68 mutants display a 20% decrease in the number of boutons in larval neuromuscular junctions and a decrease in the number of "satellite" boutons, defined as boutons that are present in the axon of a branch or that bud off from neuronal connections between two boutons.
Mutant animals exhibit increased microtubule fragmentation in neuromuscular junction synaptic boutons.
Neuronal development in the embryo proceeds normally. In larvae a reduced number of synaptic boutons are seen.
In futschK68 homozygotes, synaptic microtubules no longer form a filamentous cytoskeletal shaft that runs through the nerve terminal, and microtubule loop formation is absent. There is also a reduction in bouton number and an increase in bouton size (bouton sizes increase over two-fold). Bouton number is reduced from an average of 64.8 boutons at muscle 4 in wild-type to 37.9 in mutants. Also mutant nerve-terminals at muscle 4 rarely have branch-points. No change in muscle size is seen in these mutants.
futschK68 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Ank2XLΔ/Ank2XLΔ
futschK68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2XL-ΔR, Ank2f00518/Ank2f00518
futschK68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2XL-5xR, Ank2f00518/Ank2f00518
futschK68 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Ank2XL-20xR, Ank2f00518/Ank2f00518
futschK68 has abnormal neuroanatomy | dominant | adult stage phenotype, suppressible by spartin1/Df(3R)110
futschK68 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Ank2XL-Δall, Ank2f00518/Ank2f00518
futschK68 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Ank2XL-ΔC, Ank2f00518/Ank2f00518
futschK68/futschK68 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Ank2XLΔ
futschK68/futsch[+] is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of spartin1/Df(3R)110
futschK68/futsch[+] is a non-suppressor of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
futschK68/futschK68 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Ank2f00518
futschK68/futschK68 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Df(2R)Exel9026, P{Flo1wt.Cdk5Δ.CG30466wt.CG8204wt}
Ank2XLΔ, futschK68 has abnormal neurophysiology phenotype
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by Ank2XLΔ/Ank2XLΔ
futschK68 has microtubule | larval stage phenotype, enhanceable by Ank2XLΔ/Ank2XLΔ
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2XL-ΔR, Ank2f00518/Ank2f00518
futschK68 has microtubule | larval stage phenotype, suppressible | partially by Ank2XL-ΔR, Ank2f00518/Ank2f00518
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2XL-5xR, Ank2f00518/Ank2f00518
futschK68 has microtubule | larval stage phenotype, suppressible | partially by Ank2XL-5xR, Ank2f00518/Ank2f00518
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2XL-20xR, Ank2f00518/Ank2f00518
futschK68 has microtubule | larval stage phenotype, suppressible | partially by Ank2XL-20xR, Ank2f00518/Ank2f00518
futschK68 has adult brain phenotype, suppressible by spartin1/Df(3R)110
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Ank2XL-Δall, Ank2f00518/Ank2f00518
futschK68 has microtubule | larval stage phenotype, non-suppressible by Ank2XL-Δall, Ank2f00518/Ank2f00518
futschK68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Ank2XL-ΔC, Ank2f00518/Ank2f00518
futschK68 has microtubule | larval stage phenotype, non-suppressible by Ank2XL-ΔC, Ank2f00518/Ank2f00518
futschK68 has bouton phenotype, non-suppressible by Scer\GAL4RapGAP1-OK6/sggA81T.UAS
futschK68 has bouton phenotype, non-suppressible by Scer\GAL4futsch-C380/aPKCM.UAS
futschK68/futschK68 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2XLΔ
futschK68/futschK68 is a suppressor | partially of microtubule | larval stage phenotype of Ank2XLΔ
futschK68/futsch[+] is a suppressor of adult brain | progressive phenotype of spartin1/Df(3R)110
futschK68/futsch[+] is a suppressor of type I bouton | increased number phenotype of foxo21
futschK68/futsch[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of foxo21
futschK68/futsch[+] is a suppressor of synaptic vesicle phenotype of foxo21
futschK68/futsch[+] is a suppressor of microtubule phenotype of foxo21
futschK68 is a suppressor of bouton | increased number phenotype of Scer\GAL4RapGAP1-OK6, sggA81T.UAS
futschK68/futsch[+] is a non-suppressor of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
futschK68/futsch[+] is a non-suppressor of dendrite | pupal stage phenotype of Scer\GAL4ppk.PG, par-1HMS00405
futschK68/futschK68 is a non-suppressor of neuromuscular junction | larval stage phenotype of Ank2f00518
futschK68/futschK68 is a non-suppressor of synapse | larval stage phenotype of Ank2f00518
futschK68/futschK68 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Df(2R)Exel9026, P{Flo1wt.Cdk5Δ.CG30466wt.CG8204wt}
Ank2XLΔ, futschK68 has synapse | larval stage phenotype
Ank2XLΔ, futschK68 has NMJ bouton | larval stage phenotype
Ank2XLΔ, futschK68 has motor neuron | larval stage phenotype
Ank2XLΔ, futschK68 has axon | larval stage phenotype
The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1HMS00405 under the control of Scer\GAL4ppk.PG is not significantly modified by combination with a single copy of futschK68.
futschK68/futschK68 suppresses the aberrant microtubule aggregations in Ank2XLΔ/Ank2XLΔ mutant larval neuromuscular junctions and the axons innervating NMJs, but Ank2XLΔ/Ank2XLΔ enhances the decrease in the diameter of the microtubule core filament seen in futschK68/futschK68 mutants; futschK68/futschK68, Ank2XLΔ/Ank2XLΔ double mutants show perturbation of the area of individual synaptic boutons, either dramatically increased or reduced to small varicosities; double mutants exhibit a dramatic reduction in evoked postsynaptic potential (EPSP) amplitude, quantal content, and propagation time from stimulation artefact to the start of EPSP, but no changes in mEPSP amplitude; axon diameter of individual muscle 4 motoneurons is significantly reduced; and double mutants exhibit a significant increase in the fraction of stationary mitochondria and a decrease in anterograde transport velocities in axons, as compared to controls.
Expression of Ank2XL-ΔR, Ank2XL-5xR or Ank2XL-20xR, but not Ank2XL-Δall or Ank2XL-ΔC, in a Ank2f00518/Ank2f00518 mutant background partially rescues the decreased microtubule core filament area seen in futschK68/futschK68 mutant larval neuromuscular junctions.
futschK68/futschK68 fails to suppress the defective synaptic stability of Ank2f00518/Ank2f00518 mutant larval neuromuscular junctions.
A futschK68/+ background suppresses the increase in type 1b bouton area observed in foxo21 homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo21 mutants.
A futschK68/+ background suppresses the FM 1-43 loading defects found in foxo21 mutant synapses.
The synaptic overgrowth phenotype at the neuromuscular junction seen in third instar larvae lacking Cdk5 function (Df(2R)Exel9026 homozygotes with all deleted gene functions except Cdk5 restored using P{Flo-1wt.Cdk5Δ.CG30466wt.CG8204wt}) is not altered if the larvae are also homozygous or futschK68.
The neuromuscular junction of futschK68/+; PP2A-B'104/+ larvae have significantly larger boutons than wild-type. In contrast, either heterozygote alone has normal sized boutons.
Expression of sggA81T.Scer\UAS under the control of Scer\GAL4OK6, in a futschK68 background shows the futschK68 phenotype in which larval neuromuscular synaptic bouton number is decreased and not the opposite sggA81T.Scer\UAS overexpression phenotype.
futschK68 is partially rescued by futschN-Term.UAS/futschC-Term.UAS/Scer\GAL4da.G32
futschK68 is partially rescued by Scer\GAL4da.G32/futschN+C-Term.UAS
futschK68 is partially rescued by futschNPC-Term.UAS/Scer\GAL4da.G32
futschK68 is partially rescued by Scer\GAL4elav-C155/futschNterm.UAS
futschK68 is not rescued by futschN-Term.UAS/Scer\GAL4da.G32
futschK68 is not rescued by futschC-Term.UAS/Scer\GAL4da.G32
Expression of futschN-Term.Scer\UAS or futschC-Term.Scer\UAS separately under the control of Scer\GAL4da.G32 fails to rescue the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.
Expression of futschN-Term.Scer\UAS and futschC-Term.Scer\UAS together under the control of Scer\GAL4da.G32 partially the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.
Expression of either futschN+C-Term.Scer\UAS or futschNPC-Term.Scer\UAS separately under the control of Scer\GAL4da.G32 partially rescues the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.